scholarly journals A comparison of the performance of natural hybrids Saccharomyces cerevisiae  ×  Saccharomyces kudriavzevii at low temperatures reveals the crucial role of their S. kudriavzevii genomic contribution

2018 ◽  
Vol 274 ◽  
pp. 12-19 ◽  
Author(s):  
Guadalupe Ortiz-Tovar ◽  
Roberto Pérez-Torrado ◽  
Ana Cris Adam ◽  
Eladio Barrio ◽  
Amparo Querol
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Low Temperatures ◽  
Crucial Role ◽  
Natural Hybrids ◽  
Saccharomyces Kudriavzevii

scholarly journals Molecular Characterization of New Natural Hybrids of Saccharomyces cerevisiae and S. kudriavzevii in Brewing

2008 ◽  
Vol 74 (8) ◽  
pp. 2314-2320 ◽  
Author(s):  
Sara S. González ◽  
Eladio Barrio ◽  
Amparo Querol
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fragment Length Polymorphism ◽  
Polymorphism Analysis ◽  
Natural Hybrids ◽  
New Type ◽  
Saccharomyces Kudriavzevii ◽  
Different Origins ◽  
First Time ◽  
Restriction Fragment Length

ABSTRACT We analyzed 24 beer strains from different origins by using PCR-restriction fragment length polymorphism analysis of different gene regions, and six new Saccharomyces cerevisiae × Saccharomyces kudriavzevii hybrid strains were found. This is the first time that the presence in brewing of this new type of hybrid has been demonstrated. From the comparative molecular analysis of these natural hybrids with respect to those described in wines, it can be concluded that these originated from at least two hybridization events and that some brewing hybrids share a common origin with wine hybrids. Finally, a reduction of the S. kudriavzevii fraction of the hybrid genomes was observed, but this reduction was found to vary among hybrids regardless of the source of isolation. The fact that 25% of the strains analyzed were discovered to be S. cerevisiae × S. kudriavzevii hybrids suggests that an important fraction of brewing strains classified as S. cerevisiae may correspond to hybrids, contributing to the complexity of Saccharomyces diversity in brewing environments. The present study raises new questions about the prevalence of these new hybrids in brewing as well as their contribution to the properties of the final product.

scholarly journals Transcriptomic analysis of Saccharomyces cerevisiae x Saccharomyces kudriavzevii hybrids during low temperature winemaking

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 679 ◽  
Author(s):  
Jordi Tronchoni ◽  
Estéfani García-Ríos ◽  
Jose Manuel Guillamón ◽  
Amparo Querol ◽  
Roberto Pérez-Torrado
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Low Temperature ◽  
Differential Expression ◽  
Low Temperatures ◽  
Cold Adaptation ◽  
Interspecific Hybrids ◽  
Parental Species ◽  
Key Genes ◽  
Saccharomyces Kudriavzevii ◽  
Isolated Species

Background: Although Saccharomyces cerevisiae is the most frequently isolated species in wine fermentation, and the most studied species, other species and interspecific hybrids have greatly attracted the interest of researchers in this field in the last few years, given their potential to solve new winemaking industry challenges. S. cerevisiae x S. kudriavzevii hybrids exhibit good fermentative capabilities at low temperatures, and produce wines with smaller alcohol quantities and larger glycerol quantities, which can be very useful to solve challenges in the winemaking industry such as the necessity to enhance the aroma profile. Methods: In this study, we performed a transcriptomic study of S. cerevisiae x S. kudriavzevii hybrids in low temperature winemaking conditions. Results: The results revealed that the hybrids have acquired both fermentative abilities and cold adaptation abilities, attributed to S. cerevisiae and S. kudriavzevii parental species, respectively, showcasing their industrially relevant characteristics. For several key genes, we also studied the contribution to gene expression of each of the alleles of S. cerevisiae and S. kudriavzevii in the S. cerevisiae x S. kudriavzevii hybrids. From the results, it is not clear how important the differential expression of the specific parental alleles is to the phenotype of the hybrids. Conclusions: This study shows that the fermentative abilities of S. cerevisiae x S. kudriavzevii hybrids at low temperatures do not seem to result from differential expression of specific parental alleles of the key genes involved in this phentoype.

scholarly journals A Regulatory Role of the Rnq1 Nonprion Domain for Prion Propagation and Polyglutamine Aggregates

2008 ◽  
Vol 28 (10) ◽  
pp. 3313-3323 ◽  
Author(s):  
Hiroshi Kurahashi ◽  
Masao Ishiwata ◽  
Shoichiro Shibata ◽  
Yoshikazu Nakamura
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sodium Dodecyl Sulfate ◽  
Crucial Role ◽  
De Novo ◽  
Self Regulation ◽  
Sodium Dodecyl ◽  
Protein Conformations ◽  
Chaperone Protein ◽  
Prion Propagation

ABSTRACT Prions are infectious, self-propagating protein conformations. Rnq1 is required for the yeast Saccharomyces cerevisiae prion [PIN +], which is necessary for the de novo induction of a second prion, [PSI +]. Here we isolated a [PSI +]-eliminating mutant, Rnq1Δ100, that deletes the nonprion domain of Rnq1. Rnq1Δ100 inhibits not only [PSI +] prion propagation but also [URE3] prion and huntingtin's polyglutamine aggregate propagation in a [PIN +] background but not in a [pin −] background. Rnq1Δ100, however, does not eliminate [PIN +]. These findings are interpreted as showing a possible involvement of the Rnq1 prion in the maintenance of heterologous prions and polyQ aggregates. Rnq1 and Rnq1Δ100 form a sodium dodecyl sulfate-stable and Sis1 (an Hsp40 chaperone protein)-containing coaggregate in [PIN +] cells. Importantly, Rnq1Δ100 is highly QN-rich and prone to self-aggregate or coaggregate with Rnq1 when coexpressed in [pin −] cells. However, the [pin −] Rnq1-Rnq1Δ100 coaggregate does not represent a prion-like aggregate. These findings suggest that [PIN +] Rnq1-Rnq1Δ100 aggregates interact with other transmissible and nontransmissible amyloids to destabilize them and that the nonprion domain of Rnq1 plays a crucial role in self-regulation of the highly reactive QN-rich prion domain of Rnq1.

scholarly journals Saccharomyces cerevisiae, a Powerful Model for Studying rRNA Modifications and Their Effects on Translation Fidelity

2021 ◽  
Vol 22 (14) ◽  
pp. 7419
Author(s):  
Agnès Baudin-Baillieu ◽  
Olivier Namy
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acids ◽  
Ribosomal Rna ◽  
Crucial Role ◽  
Peptide Bond ◽  
Heterogeneous Population ◽  
Chemical Modifications ◽  
Yeast Saccharomyces Cerevisiae ◽  
Translation Fidelity

Ribosomal RNA is a major component of the ribosome. This RNA plays a crucial role in ribosome functioning by ensuring the formation of the peptide bond between amino acids and the accurate decoding of the genetic code. The rRNA carries many chemical modifications that participate in its maturation, the formation of the ribosome and its functioning. In this review, we present the different modifications and how they are deposited on the rRNA. We also describe the most recent results showing that the modified positions are not 100% modified, which creates a heterogeneous population of ribosomes. This gave rise to the concept of specialized ribosomes that we discuss. The knowledge accumulated in the yeast Saccharomyces cerevisiae is very helpful to better understand the role of rRNA modifications in humans, especially in ribosomopathies.

scholarly journals Transcriptomic analysis of Saccharomyces cerevisiae x Saccharomyces kudriavzevii hybrids during low temperature winemaking

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 679 ◽  
Author(s):  
Jordi Tronchoni ◽  
Estéfani García-Ríos ◽  
Jose Manuel Guillamón ◽  
Amparo Querol ◽  
Roberto Pérez-Torrado
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Low Temperature ◽  
Differential Expression ◽  
Low Temperatures ◽  
Cold Adaptation ◽  
Interspecific Hybrids ◽  
Parental Species ◽  
Key Genes ◽  
Saccharomyces Kudriavzevii ◽  
Isolated Species

Background: Although Saccharomyces cerevisiae is the most frequently isolated species in wine fermentation, and the most studied species, other species and interspecific hybrids have greatly attracted the interest of researchers in this field in the last few years, given their potential to solve new winemaking industry challenges. S. cerevisiae x S. kudriavzevii hybrids exhibit good fermentative capabilities at low temperatures, and produce wines with smaller alcohol quantities and larger glycerol quantities, which can be very useful to solve challenges in the winemaking industry such as the necessity to enhance the aroma profile. Methods: In this study, we performed a transcriptomic study of S. cerevisiae x S. kudriavzevii hybrids in low temperature winemaking conditions. Results: The results revealed that the hybrids have acquired both fermentative abilities and cold adaptation abilities, attributed to S. cerevisiae and S. kudriavzevii parental species, respectively, showcasing their industrially relevant characteristics. For several key genes, we also studied the contribution to gene expression of each of the alleles of S. cerevisiae and S. kudriavzevii in the S. cerevisiae x S. kudriavzevii hybrids. From the results, it is not clear how important the differential expression of the specific parental alleles is to the phenotype of the hybrids. Conclusions: This study shows that the fermentative abilities of S. cerevisiae x S. kudriavzevii hybrids at low temperatures do not seem to result from differential expression of specific parental alleles of the key genes involved in this phenotype.

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