Linearity Range Enhancement in Direct Detection of Low Concentration Uric Acid

Optik ◽  
2021 ◽  
pp. 168243
Author(s):  
Afiqah Yaacob ◽  
Nor Hafizah Ngajikin ◽  
Nurfatihah Che Abd Rashid ◽  
Maslina Yaacob ◽  
Siti Hajar Aminah Ali ◽  
...  
Author(s):  
Deng Guo Wu ◽  
David Cahen ◽  
Peter Graf ◽  
Ron Naaman ◽  
Abraham Nitzan ◽  
...  

2014 ◽  
Vol 2014 ◽  
pp. 1-19 ◽  
Author(s):  
Alina Pyka

This entry describes applications of known indicators and dyes as new visualizing reagents and various visualizing systems as well as photocatalytic reactions and bioautography method for the detection of bioactive compounds including drugs and compounds isolated from herbal extracts. Broadening index, detection index, characteristics of densitometric band, modified contrast index, limit of detection, densitometric visualizing index, and linearity range of detected compounds were used for the evaluation of visualizing effects of applied visualizing reagents. It was shown that visualizing effect depends on the chemical structure of the visualizing reagent, the structure of the substance detected, and the chromatographic adsorbent applied. The usefulness of densitometry to direct detection of some drugs was also shown. Quoted papers indicate the detection progress of selected drugs investigated by thin-layer chromatography (TLC).


MRS Advances ◽  
2020 ◽  
Vol 5 (42) ◽  
pp. 2181-2189
Author(s):  
Lucca Silva Nascimento ◽  
Erenildo Ferreira Macedo ◽  
Jéssica Aparecida Magalhães ◽  
Luísa Rodrigues Molina Dona' ◽  
Dayane Batista Tada

ABSTRACTSensors based on the surface plasmon resonance (SPR) technique are useful devices to detect and monitor interactions between biomolecules in real-time. SPR is a label-free method that monitors the variation of reflectivity of a biochip composed of a metal-coated glass prism and can be applied in several areas, such as biotechnology, food safety and clinical diagnosis. In the last years, several researchers have proven the efficiency of metallic nanoparticles (NPs) in the enhancement of SPR signal. This feature allowed the detection of biomolecules at very low concentration. Aiming to further enhance SPR signal towards the detection of proteins at low concentration and by a simple procedure, the present work compared the performance of gold and platinum bimetallic NPs (AuPtNPs) with that of monometallic gold NPs (AuNPs) in the enhancement of SPR signal. In order to evaluate the NPs, protein peanut agglutin (PNA) was used as target analyte and anti-PNA antibody was used as sensing molecule. Firstly NPs were functionalized with anti-PNA antibody and incubated with a solution containing PNA. Then, the NPs bound to PNA were injected into the SPR equipment containing a biochip previously modified with anti-PNA antibody. The results demonstrated that the AuPtNPs provided a 91-fold increase compared to the direct detection of free PNA in solution. In comparison with AuNPs, the signal generated by AuPtNPs was about 4 times higher. This encouraging result indicated that the application of bimetallic NPs may be a better strategy to further enhance sensitivity of SPR biosensors and could drive the development of new strategies that are not only simple, but also able to detect proteins at low concentrations, which is of great importance, especially in clinical diagnostics.


1997 ◽  
Vol 161 ◽  
pp. 299-311 ◽  
Author(s):  
Jean Marie Mariotti ◽  
Alain Léger ◽  
Bertrand Mennesson ◽  
Marc Ollivier

AbstractIndirect methods of detection of exo-planets (by radial velocity, astrometry, occultations,...) have revealed recently the first cases of exo-planets, and will in the near future expand our knowledge of these systems. They will provide statistical informations on the dynamical parameters: semi-major axis, eccentricities, inclinations,... But the physical nature of these planets will remain mostly unknown. Only for the larger ones (exo-Jupiters), an estimate of the mass will be accessible. To characterize in more details Earth-like exo-planets, direct detection (i.e., direct observation of photons from the planet) is required. This is a much more challenging observational program. The exo-planets are extremely faint with respect to their star: the contrast ratio is about 10−10at visible wavelengths. Also the angular size of the apparent orbit is small, typically 0.1 second of arc. While the first point calls for observations in the infrared (where the contrast goes up to 10−7) and with a coronograph, the latter implies using an interferometer. Several space projects combining these techniques have been recently proposed. They aim at surveying a few hundreds of nearby single solar-like stars in search for Earth-like planets, and at performing a low resolution spectroscopic analysis of their infrared emission in order to reveal the presence in the atmosphere of the planet of CO H2O and O3. The latter is a good tracer of the presence of oxygen which could be, like on our Earth, released by biological activity. Although extremely ambitious, these projects could be realized using space technology either already available or in development for others missions. They could be built and launched during the first decades on the next century.


2001 ◽  
Vol 120 (5) ◽  
pp. A492-A493 ◽  
Author(s):  
E HAINDL ◽  
H BENESCH ◽  
A FINCK ◽  
V MUEHISTEIN ◽  
A LEODOLTER ◽  
...  

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