scholarly journals Susceptibility testing for aztreonam plus ceftazidime/avibactam combination: A general guidance for clinical microbiology laboratories in India

2021 ◽  
Author(s):  
Yamuna Devi Bakthavatchalam ◽  
Kamini Walia ◽  
Balaji Veeraraghavan
Keyword(s):  
Clinical Microbiology ◽  
Susceptibility Testing ◽  
General Guidance

scholarly journals CIM City: the Game Continues for a Better Carbapenemase Test

2019 ◽  
Vol 57 (7) ◽  
Author(s):  
Romney M. Humphries
Keyword(s):  
Antimicrobial Susceptibility ◽  
Clinical Microbiology ◽  
Susceptibility Testing ◽  
Clinical Care ◽  
Antimicrobial Susceptibility Testing ◽  
European Committee ◽  
Link Type ◽  
Clinical Laboratories ◽  
The Right

ABSTRACT The Clinical and Laboratory Standards Institute and European Committee on Antimicrobial Susceptibility Testing agree that carbapenemase testing is not necessary for clinical care, provided that the laboratory is up to date with current breakpoints. Nonetheless, publication on the development and modification of carbapenemase tests continues, as is the case in this issue of the Journal of Clinical Microbiology (R. W. Beresford and M. Maley, J Clin Microbiol 57:e01852-18, 2019, https://doi.org/10.1128/JCM.01852-18). This commentary explores modifications to the carbapenem inactivation method—but is this the right focus for clinical laboratories?

Mixed mould species in laboratory cultures of respiratory specimens: how should they be reported, and what are the indications for susceptibility testing?: Table 1

2011 ◽  
Vol 64 (6) ◽  
pp. 543-545 ◽  
Author(s):  
J Purcell ◽  
J McKenna ◽  
P Critten ◽  
D W Denning ◽  
I A Hassan
Keyword(s):  
Clinical Microbiology ◽  
Susceptibility Testing ◽  
Positive Culture ◽  
Invasive Disease ◽  
Clinical Samples ◽  
Transplant Recipients ◽  
Patient Case ◽  
Tertiary Centre ◽  
Risk Patients ◽  
Respiratory Specimens

AimsTo investigate how clinical microbiology laboratories should report and interpret mixed mould isolates including Aspergillus species from clinical samples and the criteria for susceptibility testing of the isolates.MethodsRetrospectively collected data from our laboratory information system of moulds isolated between January 2005 and December 2007. Patient case notes were also reviewed.ResultsA total of 502 isolates (from 273 patients) were found. 20 patients with clinical diagnosis of a probable fungal infection had mixed Aspergillus species.ConclusionsIn most instances, the isolation of Aspergillus species from non-sterile sites does not represent clinical disease, but only colonisation/contamination. However, for high-risk patients including transplant recipients, a positive culture is associated with invasive disease. Our tertiary centre routinely reports single fungal isolates and mixed cultures with appropriate comments, and those considered significant will also have susceptibility testing carried out. The correlation of culture results with clinical features can differentiate between invasive disease and contamination.

scholarly journals What happened to microbiological diagnosis in 2020 beyond COVID-19?

2021 ◽  
pp. 2-7
Author(s):  
Emilia Cercenado ◽  
Keyword(s):  
Streptococcus Pyogenes ◽  
Antimicrobial Susceptibility ◽  
Diagnostic Tests ◽  
Clinical Microbiology ◽  
Susceptibility Testing ◽  
Microbiological Diagnosis ◽  
Candida Auris ◽  
Research Activity ◽  
Global Pandemic ◽  
Diagnostic Technologies

The global pandemic of COVID-19 has had negative repercussions on the activities and research in clinical microbiology laboratories other than those related to SARS-CoV-2. Nonetheless, the research activity has also continued in other fields. In this brief review, some of the recent publications related to new diagnostic tests, methods for rapid antimicrobial susceptibility testing and for the detection of resistance genes, new diagnostic technologies, and some aspects related to old and emergent pathogens (Candida auris, Elizabethkingia spp. Streptococcus pyogenes) are summarized.

scholarly journals Building a Better Test for Piperacillin-Tazobactam Susceptibility Testing: Would that It Were So Simple (It's Complicated)

2019 ◽  
Vol 58 (2) ◽  
Author(s):  
Andrew Henderson ◽  
Romney Humphries
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Clinical Microbiology ◽  
Susceptibility Testing ◽  
Resistance Mechanisms ◽  
Performance Data ◽  
Analytical Performance ◽  
Content Type ◽  
Link Type ◽  
The Face

ABSTRACT In this issue of the Journal of Clinical Microbiology, S. García-Fernandez, Y. Bala, T. Armstrong, M. Garcia-Castillo, et al. (J Clin Microbiol 58:e01042-19, 2020, https://doi.org/10.1128/JCM.01042-19) describe the performance of a reformulated Etest for piperacillin-tazobactam. The analytical performance data are excellent, but in the face of recent emerging data on the inefficacy of piperacillin-tazobactam for certain organisms that test susceptible, the value of piperacillin-tazobactam MICs is controversial. Evaluation of MICs in the context of the modal MIC for Enterobacterales and Pseudomonas aeruginosa, the variability of MIC tests, and, possibly, resistance mechanisms is important to the optimum use of this antimicrobial.

scholarly journals The Search for a Practical Method for Colistin Susceptibility Testing: Have We Found It by Going Back to the Future?

2018 ◽  
Vol 57 (2) ◽  
Author(s):  
Michael J. Satlin
Keyword(s):  
Escherichia Coli ◽  
Bacterial Infections ◽  
Clinical Microbiology ◽  
Susceptibility Testing ◽  
Practical Method ◽  
Broth Microdilution ◽  
Excellent Performance ◽  
Gram Negative ◽  
Content Type ◽  
Carbapenem Resistant

ABSTRACTPolymyxins are relied upon for the treatment of carbapenem-resistant Gram-negative bacterial infections, but polymyxin resistance is increasing. Only broth microdilution is recommended for polymyxin susceptibility testing, but this method is impractical for most clinical microbiology laboratories. An article in this issue of theJournal of Clinical Microbiology(P. J. Simner, Y. Bergman, M. Trejo, A. A. Roberts, R. Marayan, T. Tekle, S. Campeau, A. Kazmi, D. Bell, S. Lewis, P. D. Tamma, R. Humphries, and J. A. Hindler, J Clin Microbiol 57:e01163-18, 2019,https://doi.org/10.1128/JCM.01163-18) found that colistin broth disk elution, a method that requires only colistin disks and broth, had excellent performance compared to broth microdilution for all strains exceptmcr-positiveEscherichia colistrains.

scholarly journals Evaluation of the antimicrobial susceptibility testing process in clinical microbiology laboratories at Niamey, Niger

2021 ◽  
Vol 22 (4) ◽  
pp. 448-456
Author(s):  
H. Idrissa ◽  
O. Abdoulaye ◽  
A. Yacouba ◽  
D. Alhousseini Maiga ◽  
H. Moumouni Sambo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Risk Assessment ◽  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Clinical Microbiology ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Critical Control Points ◽  
Criticality Index ◽  
Reference Strains

Background: Risk assessment is the means of identifying and evaluating potential errors or problems that may occur in testing process. The aim of this study was to perform risk assessment of antimicrobial susceptibility testing (AST) process in clinical microbiology laboratories of Niamey, Niger Republic.Methodology: We conducted a descriptive cross-sectional study from October 1 to December 31, 2019, to evaluate AST performance in seven clinical microbiology laboratories at Niamey, the capital city of Niger republic. The evaluation focused on the determination of the criticality index (CI) of each critical point (frequency of occurrence of anomalies, severity of the process anomaly, and detectability of the anomaly during the process) in the AST process and the performance of the AST through an observation sheet using two reference strains; Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213.Results: The criticality index (CI) was greater than 6 for most of the critical points related to material, medium, equipment, method and labour for the AST process in all the laboratories. A range of 18-100% errors on the inhibition zone diameters of the reference strains were observed. Major and/or minor categorization (Sensitive S, Intermediate I and Resistance R) discrepancies were found at all the laboratories for either one or both reference strains. The antibiotics most affected by the S/I/R discrepancies were trimethoprim (100%), vancomycin (100%), amoxicillin (80%) and amoxicillin + clavulanic acid (70%).Conclusion: This study showed a deficiency in the control of critical control points that impacts the performance of the AST reported by the laboratories in Niger. Corrective actions are needed to improve the performance of AST in clinical microbiology laboratories in Niger.   French title: Evaluation du processus de réalisation de l’antibiogramme dans les laboratoires d’analyses de biologie médicale de la ville de Niamey, Niger Contexte: L'évaluation des risques est le moyen d'identifier et d'évaluer les erreurs ou les problèmes potentiels qui peuvent survenir dans le processus de test. L'objectif de cette étude était de réaliser une évaluation des risques du processus d'antibiogramme (ABG) dans les laboratoires de microbiologie clinique de Niamey, en République du Niger.Méthodologie: Nous avons mené une étude transversale descriptive du 1er octobre au 31 décembre 2019 pour évaluer la performance des ABG dans sept laboratoires de microbiologie clinique à Niamey, capitale de la république du Niger. L'évaluation a porté sur la détermination de l'indice de criticité (IC) de chaque point critique (fréquence d'apparition des anomalies, gravité de l'anomalie du processus et détectabilité de l'anomalie au cours du processus) dans le processus et la performance des AGB à travers une fiche d'observation en utilisant deux souches de référence; Escherichia coli ATCC 25922 et Staphylococcus aureus ATCC 29213.Résultats: L'indice de criticité était supérieur à 6 pour la plupart des points critiques liés au matériel, au milieu, à l'équipement, à la méthode et à la main-d'oeuvre pour le processus AST dans tous les laboratoires. Une fourchette d'erreurs de 18 à 100% sur les diamètres des zones d'inhibition des souches de référence a été observée. Des écarts de catégorisation majeurs et/ou mineurs (Sensible: S, Intermédiaire: I et Résistance: R) ont été constatés dans tous les laboratoires pour l'une ou les deux souches de référence. Les  antibiotiques les plus touchés par les écarts S/I/R étaient la triméthoprime (100%), la vancomycine (100%), l'amoxicilline (80%) et l'amoxicilline + acide clavulanique (70%).Conclusion: Cette étude a montré une déficience dans le contrôle des points de contrôle critiques qui a un impact sur la performance de l'antibiogramme rapportée par les laboratoires au Niger. Des actions correctives sont nécessaires pour améliorer la performance des ABG dans les laboratoires de microbiologie clinique au Niger.

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