Pattern of Drug Resistance Among Patients of Pulmonary Tuberculosis

Mycobacterium tuberculosis is a highly studied pathogen due to public health importance. Despite this, problems like early drug resistance, diagnostics and treatment success prediction are still not fully resolved. Here, we analyze the incidence of point mutations widely used for drug resistance detection in laboratory practice and conduct comparative analysis of whole-genome sequence (WGS) for clinical M. tuberculosis strains collected from patients with pulmonary tuberculosis (PTB) and extra-pulmonary tuberculosis (XPTB) localization. A total of 72 pulmonary and 73 extrapulmonary microbiologically characterized M. tuberculosis isolates were collected from patients from 2007 to 2014 in Russia. Genomic DNA was used for WGS and obtained data allowed identifying major mutations known to be associated with drug resistance to first-line and second-line antituberculous drugs. In some cases previously described mutations were not identified. Using genome-based phylogenetic analysis we identified M. tuberculosis substrains associated with distinctions in the occurrence in PTB vs. XPTB cases. Phylogenetic analyses did reveal M. tuberculosis genetic substrains associated with TB localization. XPTB was associated with Beijing sublineages Central Asia (Beijing CAO), Central Asia Clade A (Beijing A) and 4.8 groups, while PTB localization was associated with group LAM (4.3). Further, the XPTB strain in some cases showed elevated drug resistance patterns relative to PTB isolates. HIV was significantly associated with the development of XPTB in the Beijing B0/W148 group and among unclustered Beijing isolates.

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Frequency and patterns of first- and second-line drug resistance-conferring mutations in Mycobacterium tuberculosis isolated from pulmonary tuberculosis patients in a cross-sectional study in Tigray Region, Ethiopia

Journal of Global Antimicrobial Resistance ◽

10.1016/j.jgar.2020.11.017 ◽

2021 ◽

Vol 24 ◽

pp. 6-13

Author(s):

Letemichael Negash Welekidan ◽

Eystein Skjerve ◽

Tsehaye Asmelash Dejene ◽

Mengistu Welday Gebremichael ◽

Ola Brynildsrud ◽

...

Keyword(s):

Drug Resistance ◽

Mycobacterium Tuberculosis ◽

Pulmonary Tuberculosis ◽

Cross Sectional Study ◽

Second Line ◽

Sectional Study ◽

Cross Sectional ◽

Tuberculosis Patients ◽

Tigray Region ◽

Line Drug

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Tag Array gene chip rapid diagnosis anti-tuberculosis drug resistance in pulmonary tuberculosis -a feasibility study

Tuberculosis ◽

10.1016/j.tube.2018.03.010 ◽

2018 ◽

Vol 110 ◽

pp. 96-103 ◽

Cited By ~ 1

Author(s):

Wenjie Wu ◽

Peng Cheng ◽

Jingtong Lyu ◽

Zehua Zhang ◽

jianzhong Xu

Keyword(s):

Drug Resistance ◽

Pulmonary Tuberculosis ◽

Feasibility Study ◽

Rapid Diagnosis ◽

Gene Chip

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Correlates of Treatment Outcomes and Drug Resistance among Pulmonary Tuberculosis Patients Attending Tertiary Care Hospitals of Kolkata, India

PLoS ONE ◽

10.1371/journal.pone.0109563 ◽

2014 ◽

Vol 9 (10) ◽

pp. e109563 ◽

Cited By ~ 5

Author(s):

Avranil Goswami ◽

Urmita Chakraborty ◽

Tanmay Mahapatra ◽

Sanchita Mahapatra ◽

Tapajyoti Mukherjee ◽

...

Keyword(s):

Drug Resistance ◽

Pulmonary Tuberculosis ◽

Treatment Outcomes ◽

Tertiary Care ◽

Tuberculosis Patients ◽

Tertiary Care Hospitals

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Risk factors for drug-resistant tuberculosis, the association between comorbidity status and drug-resistant patterns: a retrospective study of previously treated pulmonary tuberculosis in Shandong, China, during 2004–2019

BMJ Open ◽

10.1136/bmjopen-2020-044349 ◽

2021 ◽

Vol 11 (6) ◽

pp. e044349

Author(s):

Ning-ning Tao ◽

Yi-fan Li ◽

Wan-mei Song ◽

Jin-yue Liu ◽

Qian-yun Zhang ◽

...

Keyword(s):

Risk Factors ◽

Drug Resistance ◽

Retrospective Study ◽

Pulmonary Tuberculosis ◽

Logistic Models ◽

Smoking History ◽

Categorical Variables ◽

Drug Resistant ◽

Drug Resistant Tuberculosis ◽

Resistant Tuberculosis

ObjectiveThis study was designed to identify the risk factors for drug-resistant tuberculosis (DR-TB) and the association between comorbidity and drug resistance among retreated pulmonary tuberculosis (PTB).DesignA retrospective study was conducted among all the 36 monitoring sites in Shandong, China, over a 16-year period. Baseline characteristics were collected from the TB Surveillance System. Categorical variables were compared by Fisher’s exact or Pearson’s χ2 test. The risk factors for drug resistance were identified using univariable analysis and multivariable logistic models. The influence of comorbidity on different types of drug resistance was evaluated by performing multivariable logistic models with the covariates adjusted by age, sex, body mass index, drinking/smoking history and cavity.ResultsA total of 10 975 patients with PTB were recorded during 2004–2019, and of these 1924 retreated PTB were finally included. Among retreated PTB, 26.2% were DR-TB and 12.5% had comorbidity. Smoking (adjusted OR (aOR): 1.69, 95% CI 1.19 to 2.39), cavity (aOR: 1.55, 95% CI 1.22 to 1.97) and comorbidity (aOR: 1.44, 95% CI 1.02 to 2.02) were risk factors for DR-TB. Of 504 DR-TB, 9.5% had diabetes mellitus, followed by hypertension (2.0%) and chronic obstructive pulmonary disease (1.8%). Patients with retreated PTB with comorbidity were more likely to be older, have more bad habits (smoking, alcohol abuse) and have clinical symptoms (expectoration, haemoptysis, weight loss). Comorbidity was significantly associated with DR-TB (aOR: 1.44, 95% CI 1.02 to 2.02), overall rifampin resistance (aOR: 2.17, 95% CI 1.41 to 3.36), overall streptomycin resistance (aOR: 1.51, 95% CI 1.00 to 2.27) and multidrug resistance (aOR: 1.96, 95% CI 1.17 to 3.27) compared with pan-susceptible patients (p<0.05).ConclusionSmoking, cavity and comorbidity lead to an increased risk of drug resistance among retreated PTB. Strategies to improve the host’s health, including smoking cessation, screening and treatment of comorbidity, might contribute to the control of tuberculosis, especially DR-TB, in China.

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Drug resistance pattern of M. tuberculosis in those who developed the disease after exposure to MDR/XDR pulmonary tuberculosis

Tuberculosis and lung diseases ◽

10.21292/2075-1230-2019-97-12-57-57 ◽

2020 ◽

Vol 97 (12) ◽

pp. 57-57

Author(s):

K. A. Glebov ◽

V. A. Guseva ◽

I. A. Burmistrova ◽

O. V. Lovacheva ◽

A. G. Samoylova ◽

...

Keyword(s):

Drug Resistance ◽

Pulmonary Tuberculosis ◽

Resistance Pattern ◽

Drug Resistance Pattern

.

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Biological microchips for detection of multiple drug resistant M. tuberculosis strains isolated in Kyrgyz Republic

Russian Pulmonology ◽

10.18093/0869-0189-2008-0-3-64-66 ◽

2008 ◽

pp. 64-66

Author(s):

J. T. Isakova ◽

Z. K. Goncharova ◽

A. A. Aldashev

Keyword(s):

Drug Resistance ◽

Pulmonary Tuberculosis ◽

Gene Mutations ◽

Rpob Gene ◽

Multiple Drug ◽

Drug Resistant ◽

Kyrgyz Republic ◽

Newly Diagnosed ◽

Single Drug ◽

Multiple Drug Resistant

The aim of the study was to estimate spread of primary and secondary multiple drug resistant Mycobacterium tuberculosis (MBT) and to characterize rpoB, katG, inhA, and ahpC gene mutations of rifampicin (RIF) and isoniazid (INH) resistant MBT strains isolated from tuberculosis patients in Kyrgyz. We obtained 493 specimens from patients with pulmonary tuberculosis which were diagnosed based on clinical, X-ray, and bacteriological examination. Among them, newly diagnosed pulmonary tuberculosis was in 445 patients (90.2 %), and 48 of the patients (9.8 %) have already been treated for tuberculosis. Mutations of rpoB, KatG, inhA, and ahpC genes associated with RIF and INH resistance were detected by biological chip test. Sensitive MBT strains were detected in 47 % and resistant strains were in 53 % of the newly diagnosed patients. Single-drug resistance to RIF only was detected in 3 % of cases; resistance to INH was found in 20 %, resistance to both the drugs was detected in 30 % of the patients. In pre-treated patients single-drug resistance to RIF was defined in 4 % of cases, resistance to INH was in 8 %, resistance to both the drugs was estimated in 75 % of the patients. Therefore, we suppose that there is a high prevalence of multi-drug resistant MBT in Kyrgyz Republic: 30 % among newly diagnosed patients and 75 % among pre-treated patients. The main cause of RIF-resistance of MBT is Ser531→Leu mutation of rpoB gene, and the main cause of INHresistance is Ser315→Thr mutation of katG gene.

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