Daphnetin inhibits corneal inflammation and neovascularization on a mouse model of corneal alkali burn

2022 ◽  
Vol 103 ◽  
pp. 108434
Author(s):  
Tianye Yang ◽  
Xiaoli Wang ◽  
Liang Guo ◽  
Fang Zheng ◽  
Chun Meng ◽  
...  
Keyword(s):  
Mouse Model ◽  
Alkali Burn ◽  
Corneal Inflammation

Daphnetin Inhibits Corneal Inflammation and Neovascularization on Mouse Model of Corneal Alkali Burn

2021 ◽  
Author(s):  
Tianye Yang ◽  
Xiaoli Wang ◽  
Liang Guo ◽  
Fang Zheng ◽  
Chun Meng ◽  
...  
Keyword(s):  
Mouse Model ◽  
Alkali Burn ◽  
Corneal Inflammation

Anti-Apoptotic Properties of Mesenchymal Stem Cells in a Mouse Model of Corneal Inflammation

2021 ◽  
Author(s):  
Jan Kossl ◽  
Pavla Bohacova ◽  
Barbora Hermankova ◽  
Eliska Javorkova ◽  
Alena Zajicova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mouse Model ◽  
Corneal Inflammation

scholarly journals EFFECTS OF COWPEA (VIGNA UNGUICULATA) EXTRACT IN VEGF EXPRESSION OF CORNEAL INFLAMMATION RAT MODEL (RATTUS NOVERGICUS STRAIN WISTAR)

2017 ◽  
Vol 10 (1) ◽  
pp. 65
Author(s):  
Yuliono Trika Nur Hasan ◽  
Nanda Wahyu Anandita ◽  
Husnul Khotimah
Keyword(s):  
Experimental Study ◽  
Effective Dose ◽  
Vigna Unguiculata ◽  
Methanolic Extract ◽  
Positive Control ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Alkali Burn ◽  
Corneal Inflammation ◽  
Administration Time

Purpose : to evaluate the effects of Methanolic extract of Cowpea (Vigna unguiculata) administration on vascular endothelial growthfactor (VEGF) expression in rats with corneal-alkali induced inflammation.Methods : This was an interventional experimental study. Sixty three rats were randomly selected, 3 rats as control, 60 rats were treatedwith 1 M NaOH (as inflammation model) and administered with 25 ìM,50 ìM and 100 ìM of genistein in Cowpea (Vignaungucuilata) extract four times daily. Alkali burn by 1 M NaOH infiltration using filter paper applicated on the center cornea of righteye for 60 seconds. Aquabidest as positive control and Cowpea (Vigna ungucuilata) extract treatment perfused immediately after alkali burn.VEGF observed at 6, 24, 48, 96 and 168 hours by immunohistochemical method.Results : Each dose of Cowpea (Vigna unguiculata) extract administration significantly decreased the VEGF expression ( p = 0,00). Differences of VEGF expression based on administration time were not significant (p=0,033). Interaction between time of administrationand dose of administration had influenced the VEGF expression (R Square(r2) =24,7 %). The Linear regression between VEGF expression and dose resulted in estimated effective dose (ED) with Y = 16.486–0.079 X( Y = V E G F e x p r e s s i o n , X =d o s e o f a d m i n i s t r a t i o n ) . Considering VEGF expression in normal cornea was 12, wefound the effective dose of methanolic Vigna unguiculata extract was 61.94 ìM.Conclusion :Methanolic extract of Cowpea(Vigna unguiculata) decreased the VEGF expression on alkali burn corneal inflammationin rats but no differences in VEGF expression based on time administration. Dose of 50 ìM genistein in Methanolic extract ofCowpea (Vigna unguiculata) was close to effective dose 61.94 ìM.Keywords : VEGF expression, alkali burn, Cowpea (Vigna unguiculata) extract, cornea.

Danshensu Decreases UVB-Induced Corneal Inflammation in an Experimental Mouse Model via Oral Administration

2017 ◽  
Vol 43 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Mei-Ching Teng ◽  
Pei Chang Wu ◽  
Si-Ping Lin ◽  
Chien-Yun Wu ◽  
Ping-Hsun Wang ◽  
...  
Keyword(s):  
Mouse Model ◽  
Oral Administration ◽  
Corneal Inflammation ◽  
Experimental Mouse Model ◽  
Experimental Mouse

scholarly journals PEP-1-FK506BP inhibits alkali burn-induced corneal inflammation on the rat model of corneal alkali injury

BMB Reports ◽  
2015 ◽  
Vol 48 (11) ◽  
pp. 618-623 ◽  
Author(s):  
Dae Won Kim ◽  
Sung Ho Lee ◽  
Min Jea Shin ◽  
Kibom Kim ◽  
Sae Kwang Ku ◽  
...  
Keyword(s):  
Rat Model ◽  
Alkali Burn ◽  
Corneal Inflammation ◽  
Alkali Injury

scholarly journals Effects of TNFα receptor TNF-Rp55- or TNF-Rp75- deficiency on corneal neovascularization and lymphangiogenesis in the mouse

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0245143
Author(s):  
Anna-Karina B. Maier ◽  
Nadine Reichhart ◽  
Johannes Gonnermann ◽  
Norbert Kociok ◽  
Aline I. Riechardt ◽  
...  
Keyword(s):  
Mouse Model ◽  
Mrna Expression ◽  
Lymphatic Vessels ◽  
Corneal Neovascularization ◽  
Wild Type ◽  
Delayed Effect ◽  
Corneal Inflammation ◽  
Tnf Α ◽  
Mrna And Protein Expression

Tumor necrosis factor (TNF)α is an inflammatory cytokine likely to be involved in the process of corneal inflammation and neovascularization. In the present study we evaluate the role of the two receptors, TNF-receptor (TNF-R)p55 and TNF-Rp75, in the mouse model of suture-induced corneal neovascularization and lymphangiogenesis. Corneal neovascularization and lymphangiogenesis were induced by three 11–0 intrastromal corneal sutures in wild-type (WT) C57BL/6J mice and TNF-Rp55-deficient (TNF-Rp55d) and TNF-Rp75-deficient (TNF-Rp75d) mice. The mRNA expression of VEGF-A, VEGF-C, Lyve-1 and TNFα and its receptors was quantified by qPCR. The area covered with blood- or lymphatic vessels, respectively, was analyzed by immunohistochemistry of corneal flatmounts. Expression and localization of TNFα and its receptors was assessed by immunohistochemistry of sagittal sections and Western Blot. Both receptors are expressed in the murine cornea and are not differentially regulated by the genetic alteration. Both TNF-Rp55d and TNF-Rp75d mice showed a decrease in vascularized area compared to wild-type mice 14 days after suture treatment. After 21 days there were no differences detectable between the groups. The number of VEGF-A-expressing macrophages did not differ when comparing WT to TNF-Rp55d and TNF-Rp75d. The mRNA expression of lymphangiogenic markers VEGF-C or LYVE-1 does not increase after suture in all 3 groups and lymphangiogenesis showed a delayed effect only for TNF-Rp75d. TNFα mRNA and protein expression increased after suture treatment but showed no difference between the three groups. In the suture-induced mouse model, TNFα and its ligands TNF-Rp55 and TNF-Rp75 do not play a significant role in the pathogenesis of neovascularisation and lymphangiogenesis.

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