GW29-e1627 Inverse Relations between Atherogenesis and Circulating Selenoprotein P Concentrations in Populations with Various Selenium Status

Context: Selenium status may have direct effects on bone and indirect effects through changes in thyroid hormone sensitivity. Objective: We hypothesized that variation in selenium status in healthy euthyroid postmenopausal women is associated with differences in bone turnover, bone mineral density (BMD) and fracture susceptibility. Design: The Osteoporosis and Ultrasound Study (OPUS) is a 6-yr prospective study of fracture-related factors. Setting: The study was comprised of a population-based cohort from five European cities. Participants: A total of 2374 postmenopausal women participated. Subjects with thyroid disease and nonthyroidal illness and those receiving drugs affecting thyroid status or bone metabolism were excluded, leaving a study population of 1144. Interventions: There were no interventions. Main Outcome Measures: We measured selenium (micrograms per liter); selenoprotein P (milligrams per liter); free T4 (picomoles per liter); free T3 (picomoles per liter); TSH (milliunits per liter); bone turnover markers; BMD; and vertebral, hip, and nonvertebral fractures. Results: Higher selenium levels were associated with higher hip BMD at study entry (β = 0.072, P = 0.004) and lower levels of bone formation (osteocalcin: β = −0.101, P < 0.001; procollagen type 1 N-terminal propeptide: β = −0.074, P = 0.013) and resorption markers (C-telopeptide of type 1 collagen: β = −0.058, P = 0.050; N-telopeptide of type 1 collagen: β = −0.095, P = 0.002). Higher selenoprotein P was associated with higher hip (β = 0.113, P < 0.001) and lumbar spine BMD (β = 0.088, P = 0.003) at study entry, higher hip BMD after the 6-yr follow-up (β = 0.106, P = 0.001) and lower osteocalcin (β = −0.077, P = 0.009), C-telopeptide of type 1 collagen (β = −0.075, P = 0.012), and N-telopeptide of type 1 collagen (β = −0.110, P < 0.001). Conclusion: Selenium status is inversely related to bone turnover and positively correlated with BMD in healthy euthyroid postmenopausal women independent of thyroid status.

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Selenoprotein P in serum as a biochemical marker of selenium status

The Analyst ◽

10.1039/an9952000833 ◽

1995 ◽

Vol 120 (3) ◽

pp. 833-836 ◽

Cited By ~ 59

Author(s):

M. Persson-Moschos ◽

W. Huang ◽

T. S. Srikumar ◽

B. Åkesson ◽

S. Lindeberg

Keyword(s):

Biochemical Marker ◽

Selenoprotein P ◽

Selenium Status

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Selenoprotein P and glutathione peroxidase (EC1·11·1·9) in plasma as indices of selenium status in relation to the intake of fish

British Journal Of Nutrition ◽

10.1079/bjn19950047 ◽

1995 ◽

Vol 73 (3) ◽

pp. 455-461 ◽

Cited By ~ 30

Author(s):

W. Huang ◽

B. Åkesson ◽

B. G. Svensson ◽

A. Schütz ◽

R. F. Burk ◽

...

Keyword(s):

Environmental Medicine ◽

Glutathione Peroxidase ◽

Biological Markers ◽

Fish Consumption ◽

Total Concentration ◽

Selenoprotein P ◽

Selenium Status ◽

Apparent Effect ◽

Occupational And Environmental Medicine ◽

Se Status

Department of Occupational and Environmental Medicine, University of Lund, Lund, Sweden In Sweden fish is considered to be an important source of dietary Se. Therefore Se status was assessed in forty-one middle-aged men with widely varying fish consumption. Glutathione peroxidase (EC1·11·1·9) and selenoprotein P in plasma were measured by radioimmunoassay. Plasma Se among the men increased slightly with increasing consumption of fish, but no such increases in the concentrations of glutathione peroxidase and selenoprotein P in plasma were observed. Moreover, no correlation was found between plasma Se and glutathione peroxidase or selenoprotein P. Instead, glutathione peroxidase was significantly correlated with selenoprotein P (r0·73,P<0·001), indicating that both glutathione peroxidase and selenoprotein P were functional indicators of Se status in this group. The proportion of plasma Se located in glutathione peroxidase decreased with increasing plasma Se. The results suggest that the Se consumed from fish had no apparent effect on the amount of Se incorporated into the functional selenoproteins of plasma. It is concluded that in some cases selenoproteins may be better biological markers of Se status than the total concentration of Se.

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Selenoprotein P Concentration in Plasma is an Index of Selenium Status in Selenium-Deficient and Selenium-Supplemented Chinese Subjects

Journal of Nutrition ◽

10.1093/jn/126.1.138 ◽

1996 ◽

Vol 126 (1) ◽

pp. 138-145 ◽

Cited By ~ 142

Author(s):

Kristina E. Hill ◽

Yiming Xia ◽

Björn Åkesson ◽

Martha E. Boeglin ◽

Raymond F. Burk

Keyword(s):

Selenoprotein P ◽

Selenium Status ◽

P Concentration ◽

Chinese Subjects

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Plasma selenoprotein P levels of healthy males in different selenium status after oral supplementation with different forms of selenium

European Journal of Clinical Nutrition ◽

10.1038/sj.ejcn.1600565 ◽

1998 ◽

Vol 52 (5) ◽

pp. 363-367 ◽

Cited By ~ 48

Author(s):

M Persson-Moschos ◽

G Alfthan ◽

B Åkesson

Keyword(s):

Selenoprotein P ◽

Selenium Status ◽

Oral Supplementation ◽

Healthy Males

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Functional Biomarkers for the Selenium Status in a Human Nutritional Intervention Study

Nutrients ◽

10.3390/nu12030676 ◽

2020 ◽

Vol 12 (3) ◽

pp. 676 ◽

Cited By ~ 1

Author(s):

Sandra M. Müller ◽

Christine Dawczynski ◽

Johanna Wiest ◽

Stefan Lorkowski ◽

Anna P. Kipp ◽

...

Keyword(s):

Risk Factors ◽

Cardiovascular Diseases ◽

Intervention Study ◽

Selenium Concentration ◽

Selenoprotein P ◽

Serum Selenium ◽

Selenium Status ◽

Serum Samples ◽

Pronounced Increase ◽

Gpx Activity

Soils in Germany are commonly low in selenium; consequently, a sufficient dietary supply is not always ensured. The extent of such provision adequacy is estimated by the optimal effect range of biomarkers, which often reflects the physiological requirement. Preceding epidemiological studies indicate that low selenium serum concentrations could be related to cardiovascular diseases. Inter alia, risk factors for cardiovascular diseases are physical inactivity, overweight, as well as disadvantageous eating habits. In order to assess whether these risk factors can be modulated, a cardio-protective diet comprising fixed menu plans combined with physical exercise was applied in the German MoKaRi (modulation of cardiovascular risk factors) intervention study. We analyzed serum samples of the MoKaRi cohort (51 participants) for total selenium, GPx activity, and selenoprotein P at different timepoints of the study (0, 10, 20, 40 weeks) to explore the suitability of these selenium-associated markers as indicators of selenium status. Overall, the time-dependent fluctuations in serum selenium concentration suggest a successful change in nutritional and lifestyle behavior. Compared to baseline, a pronounced increase in GPx activity and selenoprotein P was observed, while serum selenium decreased in participants with initially adequate serum selenium content. SELENOP concentration showed a moderate positive monotonic correlation (r = 0.467, p < 0.0001) to total Se concentration, while only a weak linear relationship was observed for GPx activity versus total Se concentration (r = 0.186, p = 0.021). Evidently, other factors apart from the available Se pool must have an impact on the GPx activity, leading to the conclusion that, without having identified these factors, GPx activity should not be used as a status marker for Se.

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The choice of biomarkers determines the selenium status in young German vegans and vegetarians

British Journal Of Nutrition ◽

10.1017/s0007114510002618 ◽

2010 ◽

Vol 104 (11) ◽

pp. 1601-1604 ◽

Cited By ~ 34

Author(s):

Johanna Hoeflich ◽

Birgit Hollenbach ◽

Thomas Behrends ◽

Antonia Hoeg ◽

Hagen Stosnach ◽

...

Keyword(s):

Farm Animals ◽

Total Serum ◽

Selenoprotein P ◽

Micronutrient Deficiencies ◽

Selenium Status ◽

Milk Products ◽

Essential Trace Elements ◽

Saturation Kinetics ◽

Kinetics Of ◽

Se Status

Daily nutrition varies considerably among individuals. The number of vegetarians is increasing continuously due to ethical, environmental, religious or other reasons. There is growing concern over their nutritional status with respect to micronutrient deficiencies. Among the essential trace elements, Se is of prime importance as it is part of the active site in selenoproteins. European soil and plants are relatively poor sources of Se, while farm animals are generally supplemented with Se in order to improve their health and avoid deficiency syndromes. We therefore wondered whether German vegetarians display a measurable Se deficiency. To this end, we compared young vegetarians (n 54) and omnivores (n 53). We assessed their Se status by measuring extracellular glutathione peroxidase 3 (GPX3) activity, and concentrations of total serum Se and circulating Se-transport protein selenoprotein P (SEPP). GPX3 activities were not different between the groups, whereas both total Se and SEPP concentrations were reduced to 79·5 and 71·2 % in vegetarians compared with omnivores. When splitting the group of vegetarians into vegans (n 26) and vegetarians consuming egg and milk products (n 28), analyses of the Se-dependent biomarkers did not reveal significant differences. We conclude that low serum Se is mirrored by circulating SEPP concentrations, but not by GPX3 activities in marginally supplied individuals. The specific dietary Se sources, divergent metabolic routes of selenomethionine v. selenocysteine and the different saturation kinetics of GPX3 and SEPP probably underlie our contradictory findings. Whether German vegetarians and vegans need to be considered as a Se-deficient group depends on the biomarker chosen.

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Selenoprotein P as Biomarker of Selenium Status in Clinical Trials with Therapeutic Dosages of Selenite

Nutrients ◽

10.3390/nu12041067 ◽

2020 ◽

Vol 12 (4) ◽

pp. 1067 ◽

Cited By ~ 5

Author(s):

Ola Brodin ◽

Julian Hackler ◽

Sougat Misra ◽

Sebastian Wendt ◽

Qian Sun ◽

...

Keyword(s):

Clinical Trials ◽

Western Blot ◽

Clinical Applications ◽

Protein Isoforms ◽

Selenoprotein P ◽

Selenium Status ◽

Blood Samples ◽

Therapeutic Applications ◽

Upper Limit ◽

Se Status

Selenoprotein P (SELENOP) is an established biomarker of selenium (Se) status. Serum SELENOP becomes saturated with increasing Se intake, reaching maximal concentrations of 5–7 mg SELENOP/L at intakes of ca. 100–150 µg Se/d. A biomarker for higher Se intake is missing. We hypothesized that SELENOP may also reflect Se status in clinical applications of therapeutic dosages of selenite. To this end, blood samples from two supplementation studies employing intravenous application of selenite at dosages >1 mg/d were analyzed. Total Se was quantified by spectroscopy, and SELENOP by a validated ELISA. The high dosage selenite infusions increased SELENOP in parallel to elevated Se concentrations relatively fast to final values partly exceeding 10 mg SELENOP/L. Age or sex were not related to the SELENOP increase. Western blot analyses of SELENOP verified the results obtained by ELISA, and indicated an unchanged pattern of immunoreactive protein isoforms. We conclude that the saturation of SELENOP concentrations observed in prior studies with moderate Se dosages (<400 µg/d) may reflect an intermediate plateau of expression, rather than an absolute upper limit. Circulating SELENOP seems to be a suitable biomarker for therapeutic applications of selenite exceeding the recommended upper intake levels. Whether SELENOP is also capable of reflecting other supplemental selenocompounds in high dosage therapeutic applications remains to be investigated.

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