Endosomal recognition of Lactococcus lactis G121 and its RNA by dendritic cells is key to its allergy-protective effects

Aims. The aim of this study was to evaluate the protective effects of Er Miao San (EMS) and the regulative function of bone marrow-derived dendritic cells (BMDCs) on adjuvant arthritis (AA) in rats. Methods. The ethyl acetate part of EMS (3 g/kg, 1.5 g/kg, and 0.75 g/kg) was orally administered from day 15 after immunization to day 29. The polyarthritis index and paw swelling were measured, the ankle joint pathological changes were observed using hematoxylin-eosin (HE) staining, and the spleen and thymus index were determined. Moreover, T and B cell proliferation were determined using the CCK-8 assay. The expression of BMDC surface costimulatory molecules and inflammatory factors were determined using flow cytometry and ELISA kits, respectively. Results. Compared with the AA model rats, the ethyl acetate fraction of EMS obviously reduced paw swelling (from 1.0 to 0.7) and the polyarthritis index (from 12 to 9) P < 0.01 and improved the severity of histopathology P < 0.01 . The treatment using ethyl acetate fraction of EMS significantly reduced the spleen and thymus index P < 0.01 and inhibited T and B cell proliferation P < 0.01 . Moreover, EMS significantly modulated the expression of surface costimulatory molecules in BMDCs, including CD40, CD80, CD86, and major histocompatibility complex class II (MHC-II) P < 0.01 . The results also showed that the ethyl acetate part of EMS significant inhibited the levels of proinflammatory cytokines interleukin- (IL-) 23 tumor necrosis factor- (TNF-) α and inflammatory factor prostaglandin (PG) E2 in the supernatant of BMDCs. However, the level of anti-inflammatory cytokine IL-10 was significantly increased P < 0.01 . Conclusion. These results suggest that the ethyl acetate part of EMS has better protective effects on AA rats, probably by regulating the function of BMDCs and modulating the balance of cytokines.

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Immunomodulatory Protective Effects of Rb9 Cyclic-Peptide in a Metastatic Melanoma Setting and the Involvement of Dendritic Cells

Frontiers in Immunology ◽

10.3389/fimmu.2019.03122 ◽

2020 ◽

Vol 10 ◽

Author(s):

Fabrício C. Machado ◽

Natália Girola ◽

Vera S. C. Maia ◽

Patrícia C. Bergami-Santos ◽

Alice S. Morais ◽

...

Keyword(s):

Dendritic Cells ◽

Metastatic Melanoma ◽

Cyclic Peptide ◽

Protective Effects

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Protective effects of a novel probiotic strain, Lactococcus lactis ML2018, in colitis: in vivo and in vitro evidence

Food & Function ◽

10.1039/c8fo02301h ◽

2019 ◽

Vol 10 (2) ◽

pp. 1132-1145 ◽

Cited By ~ 12

Author(s):

Meiling Liu ◽

Xiuxia Zhang ◽

Yunpeng Hao ◽

Jinhua Ding ◽

Jing Shen ◽

...

Keyword(s):

Immune Responses ◽

Lactococcus Lactis ◽

Intestinal Microbiota ◽

Inflammatory Bowel Diseases ◽

Probiotic Strain ◽

Protective Effects ◽

Bowel Diseases ◽

Inflammatory Bowel

Multiple articles have confirmed that an imbalance of the intestinal microbiota is closely related to aberrant immune responses of the intestines and to the pathogenesis of inflammatory bowel diseases (IBDs).

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Lactococcus lactis subsp. lactis JCM 5805 stimulates plasmacytoid dendritic cells which regulate anti-viral immune system

Japanese Journal of Lactic Acid Bacteria ◽

10.4109/jslab.25.75 ◽

2014 ◽

Vol 25 (2) ◽

pp. 75-80

Author(s):

Daisuke Fujiwara

Keyword(s):

Dendritic Cells ◽

Immune System ◽

Lactococcus Lactis ◽

Plasmacytoid Dendritic Cells

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A hydroxyethyl derivative of chrysin exhibits anti-inflammatory activity in dendritic cells and protective effects against dextran sodium salt-induced colitis in mice

International Immunopharmacology ◽

10.1016/j.intimp.2019.105958 ◽

2019 ◽

Vol 77 ◽

pp. 105958 ◽

Cited By ~ 1

Author(s):

Ha-Yeon Song ◽

Woo Sik Kim ◽

Jin-Man Kim ◽

Dong-Ho Bak ◽

Jeong Moo Han ◽

...

Keyword(s):

Dendritic Cells ◽

Sodium Salt ◽

Inflammatory Activity ◽

Protective Effects ◽

Anti Inflammatory

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Protective effects of selenium nanoparticle-enriched Lactococcus lactis NZ9000 against enterotoxigenic Escherichia coli K88-induced intestinal barrier damage in mice

Applied and Environmental Microbiology ◽

10.1128/aem.01636-21 ◽

2021 ◽

Author(s):

Yue Chen ◽

Lei Qiao ◽

Xiaofan Song ◽

Li Ma ◽

Xina Dou ◽

...

Keyword(s):

Escherichia Coli ◽

Lactococcus Lactis ◽

Sodium Selenite ◽

Low Cost ◽

Intestinal Barrier ◽

Feed Additives ◽

Enterotoxigenic Escherichia Coli ◽

Protective Effects ◽

Barrier Dysfunction ◽

Wide Range

Composite microecological agents have received widespread attention due to their advantageous properties, including safety, multi-effects, and low cost. This study was conducted to evaluate the protective effects of selenium (Se) nanoparticle-enriched Lactococcus lactis NZ9000 ( L. lactis NZ9000-SeNPs) against enterotoxigenic Escherichia coli K88 (ETEC K88)-induced intestinal barrier damage in C57BL/6 mice. Oral administration of L. lactis NZ9000-SeNPs significantly increased the villi height and the number of goblet cells in the ileum, and reduced the levels of serum and ileal interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ), and increased the activities of thioredoxin reductase (TrxR) and glutathione peroxidase (GSH-Px) compared with the ETEC K88-infected group not treated with L. lactis NZ9000-SeNPs. In addition, L. lactis NZ9000-SeNPs significantly attenuated the reduction of the expression levels of occludin and claudin-1, dysbiosis of the gut microbiome, and the activation of toll-like receptor (TLR)/nuclear factor-kappa (NF-κB)-mediated signaling pathway induced by ETEC K88. These findings suggested that L. lactis NZ9000-SeNPs may be a promising and safe Se supplement for food or feed additives. Importance The beneficial effects of microecological agents have been widely proven. Se, which is nutritionally essential trace element for human and animals, is incorporated into selenoproteins that have a wide range of pleiotropic effects, ranging from antioxidant and anti-inflammatory effects. However, the sodium selenite, a common addition form of Se in feed and food, has disadvantages such as strong toxicity and low bioavailability. We investigated the protective effects of L. lactis NZ9000-SeNPs against ETEC K88-induced intestinal barrier injury in C57BL/6 mice. Our results show that L. lactis NZ9000-SeNPs effectively alleviate ETEC-K88-induced intestinal barrier dysfunction. This study highlights the importance of developing a promising and safe Se supplement for the substation of sodium selenite applied in food, feed and biomedicine.

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