Effects of a probiotic fermented milk beverage containing Lactobacillus casei strain Shirota on defecation frequency, intestinal microbiota, and the intestinal environment of healthy individuals with soft stools

2010 ◽  
Vol 110 (5) ◽  
pp. 547-552 ◽  
Author(s):  
Kazumasa Matsumoto ◽  
Toshihiko Takada ◽  
Kensuke Shimizu ◽  
Kaoru Moriyama ◽  
Koji Kawakami ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Lactobacillus Casei ◽  
Fermented Milk ◽  
Healthy Individuals ◽  
Intestinal Environment ◽  
Defecation Frequency

Intestinal Microbiota Profiles of Healthy Pre-School and School-Age Children and Effects of Probiotic Supplementation

2015 ◽  
Vol 67 (4) ◽  
pp. 257-266 ◽  
Author(s):  
Chongxin Wang ◽  
Satoru Nagata ◽  
Takashi Asahara ◽  
Norikatsu Yuki ◽  
Kazunori Matsuda ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Fermented Milk ◽  
Fecal Microbiota ◽  
School Age Children ◽  
School Age ◽  
Probiotic Supplementation ◽  
Intestinal Environment ◽  
Daily Ingestion ◽  
Probiotic Product ◽  
Fecal Ph

Objectives: This study aims to establish the baseline profile of intestinal microbiota in pre-school and school-age Japanese children and to investigate the effects of a probiotic on the microbiota. Methods: We analyzed the intestinal microbiota and investigated the effects (before, during and after the ingestion period) on intestinal microbiota and the environment of 6 months of daily ingestion of a probiotic (Lactobacillus casei strain Shirota (LcS)-fermented milk). Results: We performed an open trial in 23 children (14 boys, 9 girls; age 7.7 ± 2.4 years (mean ± SD); BMI 19.6 ± 4.6). The composition of intestinal microbiota of healthy pre-school and school-age children resembled that of adults. During probiotic supplementation, the population levels of Bifidobacterium and total Lactobacillus increased significantly, while those of Enterobacteriaceae, Staphylococcus and Clostridium perfringens decreased significantly. A significant increase in fecal concentrations of organic acids and also a decrease in fecal pH were observed during the ingestion period. However, the patterns of fecal microbiota and intestinal environment were found to revert to the baseline levels (i.e. before ingestion) within 6 months following the cessation of probiotic intake. Conclusion: Regular intake of an LcS-containing probiotic product may modify the gut microbiota composition and intestinal environment in pre-school and school-age children while maintaining the homeostasis of the microbiota.

scholarly journals Effects of Undaria pinnatifida (wakame) on the human intestinal environment

2018 ◽  
Vol 8 (10) ◽  
pp. 478 ◽  
Author(s):  
Keiko Yoshinaga ◽  
Reiko Maruya ◽  
Takashi Koikeda ◽  
Takahisa Nakano
Keyword(s):  
Intestinal Microbiota ◽  
Undaria Pinnatifida ◽  
Biological Effects ◽  
Bifidobacterium Longum ◽  
Open Label ◽  
Edible Seaweed ◽  
Intestinal Environment ◽  
History Of ◽  
Defecation Frequency ◽  
Next Generation Sequencing Ngs

Background: Undaria pinnatifida (wakame) is an edible seaweed. Wakame is a common food in the Japanese diet which exhibits various biological effects. Wakame is rich in dietary fiber. Despite the long history of its intake, changes in the intestinal environment following the ingestion of wakame are unclear.Methods: We examined the effect of a 2-week intake of wakame on defecation frequency and the intestinal microbiota of 22 healthy individuals suffering from low defecation frequency. The clinical trial was designed as an open-label study.Results: Defecation frequency, defined in terms of times per week, days per week, and volume per week significantly increased during the wakame intake period. Furthermore, based on terminal restriction fragment length polymorphism (T-RFLP), the fraction of bifidobacteria as a percentage of all fecal bacteria increased significantly during the wakame intake period. At the phylum, next-generation sequencing (NGS) revealed that the relative abundance of Actinobacteria after wakame intake significantly increased while the abundance of Bacteroidetes decreased. Moreover, species-level analyses revealed that the abundance of Bifidobacterium longum increased significantly after wakame intake. B. longum colony counts on wakame-containing medium were significantly higher than those on medium without wakame.Conclusion: These observations suggest that wakame intake improves intestinal environment and increases the fecal population of bifidobacteria, indicating that it may have prebiotic properties.Keywords: Undaria pinnatifida; wakame; bowel movement; intestinal microbiota; fiber; Bifidobacterium

Effects of heat-killed Lactobacillus kunkeei YB38 on human intestinal environment and bowel movement: a pilot study

2016 ◽  
Vol 7 (3) ◽  
pp. 337-344 ◽  
Author(s):  
T. Asama ◽  
Y. Kimura ◽  
T. Kono ◽  
T. Tatefuji ◽  
K. Hashimoto ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Intestinal Microbiota ◽  
Bowel Movement ◽  
Baseline Period ◽  
Intestinal Environment ◽  
Human Intestinal Microbiota ◽  
Defecation Frequency ◽  
Bacteroides Fragilis Group ◽  
Lactobacillus Kunkeei

It is well known that lactic acid bacteria supplementation is beneficial for intestinal conditions such as microbiota; however, the effects of killed-lactic acid bacteria on intestinal conditions are largely unclear. This study aimed to evaluate the effect of heat-killed Lactobacillus kunkeei YB38 (YB38) at a dose of approximately 10 mg/day on human intestinal environment and bowel movement. This single-blind study enrolled 29 female subjects with a low defecation frequency who consumed heat-killed YB38 at four increasing dosage levels: 0 (placebo), 2, 10, and 50 mg. Each dose was consumed daily for two weeks, with a two-week baseline period preceding the dosing-period and a two-week washout period ending the study. Observed levels of Bacteroides fragilis group significantly decreased with intake of heat-killed YB38 at ≥10 mg/day compared with levels during placebo intake (P<0.01). Faecal pH significantly decreased with 10 and 50 mg/day intake (P<0.01 and 0.05, respectively). Acetic acid levels tended to increase in faeces at the 50 mg/day dose (P<0.1). Bowel movement tended to increase in all heat-killed YB38 intake periods (P<0.1). In conclusion, heat-killed YB38 altered human intestinal microbiota at doses of ≥10 mg/day and tended to increase bowel movement at ≥2 mg/day. This is the first study to show the intestinal microbiota-altering effect of L. kunkeei and to report the bowel movement-improving effect of heat-killed lactic acid bacteria.

scholarly journals Effect of the administration of a fermented milk containing Lactobacillus casei DN-114001 on intestinal microbiota and gut associated immune cells of nursing mice and after weaning until immune maturity

2008 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
Alejandra de Moreno de LeBlanc ◽  
Cecilia A Dogi ◽  
Carolina Galdeano ◽  
Esteban Carmuega ◽  
Ricardo Weill ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Immune Cells ◽  
Lactobacillus Casei ◽  
Fermented Milk

scholarly journals Influence of Camembert consumption on the composition and metabolism of intestinal microbiota: a study in human microbiota-associated rats

2004 ◽  
Vol 92 (3) ◽  
pp. 429-438 ◽  
Author(s):  
Christophe Lay ◽  
Malène Sutren ◽  
Pascale Lepercq ◽  
Catherine Juste ◽  
Lionel Rigottier-Gois ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Dairy Products ◽  
Basal Diet ◽  
Fermented Milk ◽  
Metabolic Characteristics ◽  
Human Microbiota ◽  
Gradient Gel Electrophoresis ◽  
Metabolic Activities ◽  
Temperature Gradient Gel Electrophoresis ◽  
Micro Organisms

The objective of the present study was to evaluate the consequence of Camembert consumption on the composition and metabolism of human intestinal microbiota. Camembert cheese was compared with milk fermented by yoghurt starters andLactobacillus caseias a probiotic reference. The experimental model was the human microbiota-associated (HM) rat. HM rats were fed a basal diet (HMB group), a diet containing Camembert made from pasteurised milk (HMCp group) or a diet containing fermented milk (HMfm group). The level of micro-organisms from dairy products was measured in faeces using cultures on a specific medium and PCR–temporal temperature gradient gel electrophoresis. The metabolic characteristics of the caecal microbiota were also studied: SCFA, NH3, glycosidase and reductase activities, and bile acid degradations. The results showed that micro-organisms from cheese comprised 105–108bacteria/g faecal sample in the HMCp group.Lactobacillusspecies from fermented milk were detected in HMfm rats. Consumption of cheese and fermented milk led to similar changes in bacterial metabolism: a decrease in azoreductase activity and NH3concentration and an increase in mucolytic activities. However, specific changes were observed: in HMCp rats, the proportion of ursodeoxycholic resulting from chenodeoxycholic epimerisation was higher; in HMfm rats, α and β-galactosidases were higher than in other groups and both azoreductases and nitrate reductases were lower. The results show that, as for fermented milk, Camembert consumption did not greatly modify the microbiota profile or its major metabolic activities. Ingested micro-organisms were able to survive in part during intestinal transit. These dairy products exert a potentially beneficial influence on intestinal metabolism.

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