Leukocyte-platelet-rich plasma diminishes bone matrix deposition in rat calvaria treated with autograft due to simultaneous increase in immunohistochemical expression of Indian Hedgehog, transforming growth factor-β, and parathyroid-1 receptor

2015 ◽  
Vol 43 (8) ◽  
pp. 1470-1477 ◽  
Author(s):  
Daniel Xavier Cerci ◽  
Giovanna Schirmer Portela ◽  
Emanuelle Juliana Cunha ◽  
João Ricardo de Almeida Grossi ◽  
João Cesar Zielak ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Platelet Rich Plasma ◽  
Bone Matrix ◽  
Transforming Growth Factor Β ◽  
Simultaneous Increase ◽  
Indian Hedgehog ◽  
Immunohistochemical Expression ◽  
Matrix Deposition ◽  
Rat Calvaria

scholarly journals Basic Studies on the Clinical Applications of Platelet-Rich Plasma

2003 ◽  
Vol 12 (5) ◽  
pp. 509-518 ◽  
Author(s):  
Masaki Yazawa ◽  
Hisao Ogata ◽  
Tatsuo Nakajima ◽  
Taisuke Mori ◽  
Naohide Watanabe ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Fibrin Glue ◽  
Transforming Growth Factor ◽  
Platelet Rich Plasma ◽  
Transforming Growth Factor Β ◽  
Clinical Applications ◽  
Platelet Concentrate ◽  
Platelet Concentrates ◽  
Basic Studies

Platelets, which contain many growth factors such as platelet-derived growth factor (PDGF) and transforming growth factor-β (TGF-β), are being used in clinical applications as platelet-rich plasma (PRP). Only a few studies, however, have been conducted on the growth factors present in PRP and on the clinical applications using the drug delivery system (DDS). For the purpose of clinical application, we first modified the PRP preparation method and assessed the amounts of growth factors contained in the human platelet concentrates. Furthermore, we assessed fibrin glue as a DDS of platelet concentrates. Platelet precipitations were made by twice centrifuging human whole blood. The precipitated platelet was resuspended to yield the platelet concentrates. The growth factor concentrations were measured. Fibrin glue sheets containing this platelet concentrate were implanted in rabbit pinna and samples were obtained for immunostaining (anti-PDGF antibody) to assess the use of PRP over time using the fibrin glue as the DDS. The mean concentration of growth factors present in the platelet concentrates was three times or greater than that of conventional PRP. Furthermore, the results indicated that when the platelet concentrate was used with fibrin glue as a carrier, the contents were released over a period of about 1 week. This raises the possibility that this system may be useful in clinical applications.

Effect of transforming growth factor-β on rat calvaria

Bone ◽  
1992 ◽  
Vol 13 (5) ◽  
pp. A11-A11
Author(s):  
R. Fujimoto ◽  
H.E. Takahashi ◽  
T. Tanizawa ◽  
N. Yamamoto ◽  
K. Tokunaga
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Rat Calvaria

scholarly journals Platelet Rich Plasma (PRP) Produces an Atherofibrotic Histophenotype During Craniofacial Bone Repair Due to Changes of Immunohistochemical Expression of Erk1/2, p38α/β, Adiponectin and Elevated Presence of Cells Exhibiting B-scavenger Receptor (CD36+)

2016 ◽  
Vol 27 (3) ◽  
pp. 243-254 ◽  
Author(s):  
Caroline Cristine Schroeder ◽  
Juliana Souza Vieira, Rafaela Scariot ◽  
João Cesar Zielak, Geraldo Monteiro Ribeiro ◽  
Tatiana Miranda Deliberador ◽  
Andrea M. Marcaccini, Allan Fernando Giovanini
Keyword(s):  
Bone Repair ◽  
Cellular Proliferation ◽  
Platelet Rich Plasma ◽  
Bone Matrix ◽  
Scavenger Receptor ◽  
Foam Cell Formation ◽  
Immunohistochemical Expression ◽  
Craniofacial Bone ◽  
Matrix Deposition ◽  
Post Surgery

Abstract The platelet-extracellular matrix interaction in platelet rich plasma (PRP) through thrombospondin receptor-CD36 induces the secretion of growth factors responsible for cellular proliferation and differentiation during the repair process. Since CD36 also acts as a class B-scavenger-receptor for development of foam-like cells and mitogen-activated kinases, such as Erk1/2 and p38α/β, are important proteins activated by platelet growth factor, the aim of this study was to evaluate the immunohistochemical presence of CD36, Erk1/2, p38α/β during the bone repair treated and non-treated with PRP and to compare these results with the histomorphometry of repair. Simultaneously, the immunopresence of adiponectin was analyzed, which may contribute to osteogenesis at the same time it inhibits fibrosis and impairs adipogenesis and foam cell formation in the medullary area. An artificial bone defect measuring 5×1 mm was produced in the calvaria of 56 Wistar rats. The defects were randomly treated with autograft, autograft+PRP, PRP alone and sham. The animals were euthanized at 2 and 6 weeks post-surgery. Data were analyzed by ANOVA followed by non-parametric test Student Newman-Keuls (p<0.05) for histomorphometric and immunohistochemical interpretation. The results revealed that in specimens that received PRP the immunopositivity for Erk1/2, p38α/β and CD36 proteins increased significantly while the immunohistochemical expression of adiponectin decreased simultaneously. There was also an accentuated reduction of bone matrix deposition and increase of the medullary area represented by fibrosis and/or presence of foam-like cells, which exhibited immunophenotype CD36+adiponectin. The findings of this study suggest that PRP acted as an inhibitor of osteogenesis during the craniofacial bone repair and induced a pathological condition that mimics an atherofibrotic condition.

Effects of platelet rich plasma on the gastric serosal surface neomucosa formation: an experimental rodent model

2018 ◽  
Vol 44 (1) ◽  
pp. 32-40
Author(s):  
Binboğa Sinan ◽  
Kasapoğlu Pınar ◽  
Binboğa Elif ◽  
Cikot Murat ◽  
Baytekin Fırat ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Transforming Growth Factor Β ◽  
Treated Group ◽  
En Bloc Resection ◽  
Bloc Resection ◽  
En Bloc ◽  
Platelet Concentration

Abstract Background Autologous platelet rich plasma (PRP) is the platelet concentration obtained from thrombocytes in the plasma. During the healing process, the platelets are activated and then release the granules which stimulate the inflammatory cascade and healing process. Platelet derived growth factor, vascular endothelial growth factor (VEGF), transforming growth factor β (TGFβ), epidermal growth factor (EGF) and fibroblast growth factor (FGF) are valuable markers used for cell regeneration. The aim of this study was to investigate the potential effects of PRP treatment on the neomucosa formation, a potential technique for increasing the intestinal surface area in patients with short bowel syndrome (SBS). Materials and methods Thirty-two male Wistar-Hannover rats were divided into: sham, control, PRP-treated and last group for PRP preparation (n=8). Plasma levels of VEGF, TGFβ, EGF and FGF were quantified by ELISA. En-bloc resection of anastomotic part was performed and stained with hematoxylin-eosin. Results VEGF, FGF, TGFβ and EGF levels were found significantly increased in PRP-treated group compared to others (p<0.001). Neomucosa formation was observed in experimental groups but the area increased significantly in PRP group, compared to other groups (p<0.001). Conclusion PRP therapy in gastrointestinal anastomoses is truly beneficial and surgically applicable treatment in SBS patients.

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