Effect of nitrogen application rate on content of glutenin macropolymer and high molecular weight glutenin subunits in grains of two winter wheat cultivars

SUMMARYTwo winter wheat (Triticum aestivum L.) cultivars, Jimai20 and Shannong12, differing in phosphorus (P) utilization efficiency, were selected to study the effect of P application rate on changes in glutenin macropolymer (GMP) size distribution and the content of high-molecular-weight glutenin subunits (HMW-GS) in wheat grain. Four P levels (0, 40, 100 and 160 kg/ha) were applied under N1 (112·5 kg/ha) and N2 (225 kg/ha) conditions in the field, in 2008/09 and 2009/10. The results showed that increased P levels favoured HMW-GS synthesis under N1 conditions, but had a less pronounced effect under N2. When compared with the control, the volume proportions of <10 μm GMP particles in grains of both cultivars were significantly decreased, and those of >100 μm were increased in response to P application. The volume proportions of 10–100 μm GMP particles in the cultivars Jimai20 and Shannong12 were respectively lower and higher in response to P application than with no P fertilizer. At maturity, for both cultivars, total HMW-GS content was negatively correlated with GMP particle volume of <10 μm, but positively correlated with that of >100 μm. These observations suggest that both P and N affect protein synthesis in wheat grains and there exists a relationship between HMW-GS content and the synthesis of large GMP particles (>100 μm). The N×P interaction was the most important factor to regulate the HMW-GS and GMP contents.

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The accumulation of high molecular weight glutenin subunits (HMW-GS) and their relation to dough rheological quality in Chinese winter wheat

Australian Journal of Agricultural Research ◽

10.1071/ar05080 ◽

2006 ◽

Vol 57 (1) ◽

pp. 41 ◽

Cited By ~ 2

Author(s):

Zhiying Deng ◽

Jichun Tian ◽

Ruibo Hu

Keyword(s):

Molecular Weight ◽

Winter Wheat ◽

High Molecular Weight ◽

Grain Development ◽

Glutenin Subunits ◽

Sds Page ◽

Winter Wheat Cultivars ◽

The Individual ◽

Accumulation Trend ◽

Days After Anthesis

Six winter wheat cultivars were categorised into high gluten, medium gluten, and low gluten according to their protein content and gluten index. The object of this study was to determine the accumulation of high molecular weight glutenin subunits (HMW-GS) and their relationship to dough rheological quality. They were grown in 3 replicates on experimental plots at the Shandong Agricultural University research farm in 2001. HMW-GS compositions during grain development were investigated by SDS-PAGE procedures, followed by imaging densitometry to determine quantitative variations. Initial formation time of HMW-GS was different among cultivars. HMW-GS in cultivars with high gluten had formed completely by 10 days after anthesis, but were still only partially formed at this time in cultivars having weak gluten. Accumulation quantities of HMW-GS followed with grain development. Individual HMW-GS accumulated rapidly between 25 days after anthesis and maturity. The kinetic accumulation trend for the individual HMW-GS was similar in the same type of cultivars, but quantities were different. Strong cultivars had more kinetic accumulation quantities of HMW-GS than weak cultivars. HMW-GS kinetic accumulation quantities during grain development were significantly positively correlated with dough rheological characteristics and SDS-sedimentation volume.

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