Characterization of AT1 and AT2 receptor expression profiles in human skin during fetal life

2007 ◽  
Vol 46 (3) ◽  
pp. 221-225 ◽  
Author(s):  
Hong-Wei Liu ◽  
Biao Cheng ◽  
Xiao-Bing Fu ◽  
Jian-Fu Li ◽  
Tong-Zhu Sun
Keyword(s):  
Human Skin ◽  
Expression Profiles ◽  
Receptor Expression ◽  
At2 Receptor ◽  
Fetal Life

Characterization of liver X receptor expression and function in human skin and the pilosebaceous unit

2007 ◽  
Vol 16 (10) ◽  
pp. 844-852 ◽  
Author(s):  
Louise E. Russell ◽  
Wesley J. Harrison ◽  
Adiam W. Bahta ◽  
Christos C. Zouboulis ◽  
Jacky M. Burrin ◽  
...  
Keyword(s):  
Human Skin ◽  
Liver X Receptor ◽  
Receptor Expression ◽  
Pilosebaceous Unit ◽  
And Function

scholarly journals Characterization of Human Adrenal Steroidogenesis During Fetal Development

2018 ◽  
Vol 104 (5) ◽  
pp. 1802-1812 ◽  
Author(s):  
Cecilie Melau ◽  
John Erik Nielsen ◽  
Hanne Frederiksen ◽  
Karen Kilcoyne ◽  
Signe Perlman ◽  
...  
Keyword(s):  
Expression Profiles ◽  
First Trimester ◽  
Distinct Pattern ◽  
Endocrine Function ◽  
Fetal Life ◽  
Second Trimester ◽  
Male And Female ◽  
Adrenal Steroid ◽  
Adrenal Steroidogenesis

Abstract Context The endocrine function of human fetal adrenals (HFAs) is activated already during first trimester, but adrenal steroidogenesis during fetal life is not well characterized. Objective This study aimed to investigate HFA steroidogenesis by analyzing adrenal glands from first and second trimesters. Design and Setting Male and female HFA from gestational weeks (GWs) 8 to 19 were examined, including a total of 101 samples from 83 fetuses. Main Outcome Measure(s) Expression level of steroidogenic genes and protein expression/localization were determined by quantitative PCR and immunohistochemistry, respectively, and intra-adrenal steroid levels were quantified by LC-MS/MS. Results Transcriptional levels of StAR, CYP11A1, CYP17A1, CYP21A2, CYP11B1/2, and SULT2A1 were significantly higher in second trimester compared to first trimester (P < 0.05), whereas expression levels of 3β-HSD2 and ARK1C3 were unaltered between GWs 8 and 19. All investigated steroidogenic proteins were expressed in a distinct pattern throughout the investigated period, with most enzymes expressed primarily in the fetal zone, except 3β-HSD1/2, which was expressed mainly in the definitive zone. Abundant steroidogenic enzyme expression was reflected in overall high intra-adrenal tissue concentrations of mineralocorticoids, glucocorticoids, and androgens; cortisol was the most abundant (1071 to 2723 ng/g tissue), and testosterone levels were the lowest (2 to 14 ng/g tissue). Conclusions The expression profiles of HFA steroidogenic enzymes are distinct from first to second trimester, with no major differences between male and female samples. Intra-adrenal steroid hormone concentrations confirm that cortisol is produced throughout first and second trimesters, suggesting continued regulation of the hypothalamus-pituitary-adrenal axis during this entire period.

scholarly journals Influence of AT1 Receptor Activity on AT1 and AT2 Receptor Expression During Fetal Life. † 284

1997 ◽  
Vol 41 ◽  
pp. 50-50
Author(s):  
Jean A. Petershack ◽  
Margaret E. Samyn ◽  
Jeffrey L. Segar ◽  
Jean E. Robillard
Keyword(s):  
At1 Receptor ◽  
Receptor Expression ◽  
Receptor Activity ◽  
At2 Receptor ◽  
Fetal Life

scholarly journals Functional characterization of a full length pregnane X receptor, expression in vivo, and identification of PXR alleles, in Zebrafish (Danio rerio)

2013 ◽  
Vol 142-143 ◽  
pp. 447-457 ◽  
Author(s):  
Afonso C.D. Bainy ◽  
Akira Kubota ◽  
Jared V. Goldstone ◽  
Roger Lille-Langøy ◽  
Sibel I. Karchner ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Functional Characterization ◽  
Pregnane X Receptor ◽  
Full Length ◽  
Receptor Expression

Characterization of Nucleotide Binding ­Site-Encoding Genes in Sweetpotato, Ipomoea batatas(L.) Lam., and Their Response to Biotic and Abiotic Stresses

2021 ◽  
pp. 1-15
Author(s):  
Zengzhi Si ◽  
Yake Qiao ◽  
Kai Zhang ◽  
Zhixin Ji ◽  
Jinling Han
Keyword(s):  
Binding Site ◽  
Abiotic Stresses ◽  
Ipomoea Batatas ◽  
Expression Profiles ◽  
Nucleotide Binding ◽  
Regulatory Elements ◽  
Nucleotide Binding Site ◽  
Biotic And Abiotic Stresses ◽  
Encoding Genes

Sweetpotato, <i>Ipomoea batatas</i> (L.) Lam., is an important and widely grown crop, yet its production is affected severely by biotic and abiotic stresses. The nucleotide binding site (NBS)-encoding genes have been shown to improve stress tolerance in several plant species. However, the characterization of NBS-encoding genes in sweetpotato is not well-documented to date. In this study, a comprehensive analysis of NBS-encoding genes has been conducted on this species by using bioinformatics and molecular biology methods. A total of 315 NBS-encoding genes were identified, and 260 of them contained all essential conserved domains while 55 genes were truncated. Based on domain architectures, the 260 NBS-encoding genes were grouped into 6 distinct categories. Phylogenetic analysis grouped these genes into 3 classes: TIR, CC (I), and CC (II). Chromosome location analysis revealed that the distribution of NBS-encoding genes in chromosomes was uneven, with a number ranging from 1 to 34. Multiple stress-related regulatory elements were detected in the promoters, and the NBS-encoding genes’ expression profiles under biotic and abiotic stresses were obtained. According to the bioinformatics analysis, 9 genes were selected for RT-qPCR analysis. The results revealed that <i>IbNBS75</i>, <i>IbNBS219</i>, and <i>IbNBS256</i> respond to stem nematode infection; <i>Ib­NBS240</i>, <i>IbNBS90</i>, and <i>IbNBS80</i> respond to cold stress, while <i>IbNBS208</i>, <i>IbNBS71</i>, and <i>IbNBS159</i> respond to 30% PEG treatment. We hope these results will provide new insights into the evolution of NBS-encoding genes in the sweetpotato genome and contribute to the molecular breeding of sweetpotato in the future.

scholarly journals Characterization of Two Ethephon-Induced IDA-Like Genes from Mango, and Elucidation of Their Involvement in Regulating Organ Abscission

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 439
Author(s):  
Avinash Chandra Rai ◽  
Eyal Halon ◽  
Hanita Zemach ◽  
Tali Zviran ◽  
Isaac Sisai ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Mangifera Indica ◽  
Ectopic Expression ◽  
Fruit Trees ◽  
Floral Organ ◽  
Cytosolic Ph ◽  
Early Increase ◽  
Encoding Genes ◽  
Fruitlet Abscission

In mango (Mangifera indica L.), fruitlet abscission limits productivity. The INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) peptide acts as a key component controlling abscission events in Arabidopsis. IDA-like peptides may assume similar roles in fruit trees. In this study, we isolated two mango IDA-like encoding-genes, MiIDA1 and MiIDA2. We used mango fruitlet-bearing explants and fruitlet-bearing trees, in which fruitlets abscission was induced using ethephon. We monitored the expression profiles of the two MiIDA-like genes in control and treated fruitlet abscission zones (AZs). In both systems, qRT-PCR showed that, within 24 h, both MiIDA-like genes were induced by ethephon, and that changes in their expression profiles were associated with upregulation of different ethylene signaling-related and cell-wall modifying genes. Furthermore, ectopic expression of both genes in Arabidopsis promoted floral-organ abscission, and was accompanied by an early increase in the cytosolic pH of floral AZ cells—a phenomenon known to be linked with abscission, and by activation of cell separation in vestigial AZs. Finally, overexpression of both genes in an Atida mutant restored its abscission ability. Our results suggest roles for MiIDA1 and MiIDA2 in affecting mango fruitlet abscission. Based on our results, we propose new possible modes of action for IDA-like proteins in regulating organ abscission.

scholarly journals Creation of Ionically Crosslinked Tri-Layered Chitosan Membranes to Simulate Different Human Skin Properties

Materials ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1807
Author(s):  
Rocío Guerle-Cavero ◽  
Blanca Lleal-Fontàs ◽  
Albert Balfagón-Costa
Keyword(s):  
Elastic Modulus ◽  
Human Skin ◽  
Sodium Tripolyphosphate ◽  
Snow Crab ◽  
Suitable Material ◽  
Cosmetic Industry ◽  
Novel Method ◽  
Current Article ◽  
Chitosan Membranes

In 2023, new legislation will ban the use of animals in the cosmetic industry worldwide. This fact, together with ethical considerations concerning the use of animals or humans in scientific research, highlights the need to propose new alternatives for replacing their use. The aim of this study is to create a tri-layered chitosan membrane ionically crosslinked with sodium tripolyphosphate (TPP) in order to simulate the number of layers in human skin. The current article highlights the creation of a membrane where pores were induced by a novel method. Swelling index, pore creation, and mechanical property measurements revealed that the swelling index of chitosan membranes decreased and, their pore formation and elasticity increased with an increase in the Deacetylation Grade (DDA). Additionally, the results demonstrate that chitosan’s origin can influence the elastic modulus value and reproducibility, with higher values being obtained with seashell than snow crab or shrimp shells. Furthermore, the data show that the addition of each layer, until reaching three layers, increases the elastic modulus. Moreover, if layers are crosslinked, the elastic modulus increases to a much greater extent. The characterization of three kinds of chitosan membranes was performed to find the most suitable material for studying different human skin properties.

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