Essential oils of Salvia bracteata and Salvia rubifolia from Lebanon: Chemical composition, antimicrobial activity and inhibitory effect on human melanoma cells

Eleven known triterpenes (α-amyrin, β-amyrin, lupeol, and their respective acetates, 3- O-acetyl derivatives of betulinic, oleanolic, and ursolic acids, cycloartenol, and tirucall-7,24-dienol), two new flavonols presenting an uncommon interglycosidic O-(1→3) linkage (kaempferol 3- O-α-L-arabinofuranosyl-(1→3)-α-L-rhamnoside and quercetin 3- O-α-L-arabinofuranosyl-(1→3)-α-L-rhamnoside), β-sitosterol, stigmasterol, quercetin, and gallic acid were isolated from the Amazonian medicinal mistletoe, Cladocolea micrantha Kuijt (Loranthaceae). Their structures were established by spectral methods and eventual chromatographic comparisons. The quercetin derivative was not cytotoxic to MV3 human melanoma cells, but was able, when administered at 1 μg/mL, to promote a twofold inhibition of the migration of the cells through the transwell system when compared with paclitaxel at 5 μM.

Download Full-text

Comparative phytochemical profile and antiproliferative activity on human melanoma cells of essential oils of three lebanese Salvia species

Industrial Crops and Products ◽

10.1016/j.indcrop.2015.12.080 ◽

2016 ◽

Vol 83 ◽

pp. 492-499 ◽

Cited By ~ 20

Author(s):

Alessandra Russo ◽

Carmen Formisano ◽

Daniela Rigano ◽

Venera Cardile ◽

Nelly Apostolides Arnold ◽

...

Keyword(s):

Essential Oils ◽

Antiproliferative Activity ◽

Melanoma Cells ◽

Human Melanoma ◽

Human Melanoma Cells ◽

Phytochemical Profile

Download Full-text

The Influence of Extracts from Common Houseleek (Sempervivum tectorum) on the Metabolic Activity of Human Melanoma Cells WM-266-4

Processes ◽

10.3390/pr9091549 ◽

2021 ◽

Vol 9 (9) ◽

pp. 1549

Author(s):

Maša Knez Marevci ◽

Sašo Bjelić ◽

Barbara Dariš ◽

Željko Knez ◽

Maja Leitgeb

Keyword(s):

Cell Line ◽

Metabolic Activity ◽

Soxhlet Extraction ◽

Extraction Methods ◽

Inhibitory Effect ◽

Melanoma Cells ◽

Human Melanoma ◽

Specific Cell ◽

Incurable Cancer ◽

Human Melanoma Cells

Human melanoma cells are known as one of the most aggressive cancer cells, and consequently, melanoma is one of the most incurable cancer diseases. There is intense activity in research and development of potential medicines for malignant diseases, including alternative forms of remedies. Therefore, the purpose of our work was testing extracts from the common houseleek (Sempervivum tectorum) grown in Slovenia to establish its impact on human melanoma cells. Namely, we wanted to verify if the extracts inhibit growth of malignant cells and their metabolic activity. Soxhlet, cold solvent, ultrasound, and supercritical extraction methods were applied to obtain S. tectorum extracts. Polyphenols and proanthocyanins content in acquired extracts was determined as well as their antioxidative potential. For a relevant comparison, Chinese (CHI) dried and Slovenian (SLO) lyophilized S. tectorum was used. Results showed that the highest contents of polyphenols and proanthocyanins were yielded from lyophilized material, which also had the highest antioxidative potential. The focus of our work was on analysis of possible inhibition effects of the extracts on human melanoma cells since no past studies were found regarding the possible effects of S. tectorum on metabolic activity of WM-266-4. We established that in a 24-h incubation period, the extracts inhibited metabolic activity of the cells at their concentrations of 20, 10, 4, 2, 1, 0.2, and 0.02 mg/mL. Extract obtained from SLO S. tectorum (ultrasound extraction with acetone as a solvent), which showed promising results of inhibitory effect on the mentioned cells, was further described since the local plant was the focus of our study. CHI S. tectorum extract (Soxhlet extraction with ehtanol:water mixture = 1:1 as a solvent) showed the highest inhibitory effect on human melanoma cells WM-266-4, although both obtained extracts are suitable for their growth inhibition of this specific cell line. Our results suggest inhibitory ability of S. tectorum extracts on the metabolic activity of WM-266-4 metastatic cell line, suggesting their potential use as an anticancer agent.

Download Full-text

MIR-520f Regulated Itch Expression and Promoted Cell Proliferation in Human Melanoma Cells

Dose-Response ◽

10.1177/1559325820918450 ◽

2020 ◽

Vol 18 (2) ◽

pp. 155932582091845 ◽

Cited By ~ 4

Author(s):

Ming-xia Sun ◽

Qun An ◽

La-mei Chen ◽

Ling Guo

Keyword(s):

Cell Proliferation ◽

Melanoma Cell ◽

Circular Rna ◽

Inhibitory Effect ◽

Melanoma Cells ◽

Human Melanoma ◽

Human Melanoma Cell ◽

Human Melanoma Cells ◽

Potential Biomarker ◽

Growth Assay

Accumulating evidence suggests that abnormal expression and dysfunction of microRNA is involved in development of cancers. However, the function of miR-520f especially in human melanoma remains elusive. In the current study, the underlying function of miR-520f in human melanoma was investigated. Our study demonstrated that the miR-520f level in human melanoma cell lines and clinical tissues was increased. Overexpression of miR-520f promoted cell proliferation by using the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation, anchorage-independent growth assay, and 5-bromo-2-deoxyuridine assays. Furthermore, we revealed that miR-520f could interact with circular RNA Itchy E3 ubiquitin protein ligase (ITCH) 3′-untranslated region and suppress ITCH expression in human melanoma cells. The inhibitory effect of miR-520f-in could be partially restored by knockdown of ITCH in human melanoma cells. In summary, this study provides novel insights into miR-520f act as a crucial role in the regulation of human melanoma cell growth via regulating ITCH, which might be a potential biomarker and therapeutic target of human melanoma.

Download Full-text

Inhibitory effect of salmosin, a Korean snake venomderived disintegrin, on the integrin αv-mediated proliferation of SK-Mel-2 human melanoma cells

Journal of Pharmacy and Pharmacology ◽

10.1211/0022357022160 ◽

2003 ◽

Vol 55 (11) ◽

pp. 1577-1582 ◽

Cited By ~ 17

Author(s):

Kwang-Hoe Chung ◽

Sung-Hoon Kim ◽

Kyu-yeon Han ◽

Young-Doug Sohn ◽

Soo-Ik Chang ◽

...

Keyword(s):

Inhibitory Effect ◽

Melanoma Cells ◽

Human Melanoma ◽

Integrin Αv ◽

Human Melanoma Cells

Download Full-text

Antibacterial Activity and Chemical Composition of Essential Oils from Some Galium (Rubiaceae) Species Against Pathogenic Bacteria

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v5i11.1330-1333.1414 ◽

2017 ◽

Vol 5 (11) ◽

pp. 1330

Author(s):

Filiz Yağız ◽

Rifat Battaloğlu ◽

Sedef İlk ◽

Ahmet Savran

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Pseudomonas Syringae ◽

Pathogenic Bacteria ◽

Inhibitory Effect ◽

E Coli ◽

Gram Positive Bacteria ◽

Potent Inhibitory Effect

In this work, chemical composition and antimicrobial activity of the essential oils of Galium incanum, Galium dieckii ve Galium aladaghense were firstly reported. The essential oils were obtained from the all parts of the plant by hydrodistillation and analyzed by using GC-MS. Antimicrobial activity of synthezied essential oils was carried out against 5 pathogen bacteria Escherichia coli (E. coli) ATCC 25922, Staphylococcus aureus (S. aureus) ATCC 25923, Pseudomonas syringae pv. tomato (P. syringae) DC300, Salmonella enterica serotype Typhmurium (S. typhmurium) SL 1344 and Streptococcus mutans (S. mutans) ATCC 25175. According to the results, it was determined that isolated essential oils comprised of 61 compounds. Compounds of essential oils included that structure monoterpene (8.2%), monoterpenoid (14.75%) and sesquiterpene (14.75%). Unclassified compounds have been identified as other compounds. From the antimicrobial activity was observed that the isolated essential oil from Galium incanum, Galium dieckii ve Galium aladaghense exhibited a potent inhibitory effect against all gram negative and gram positive bacteria with diameter of inhibition zones ranging from 4.3 to 12.3 mm. Essential oil of Galium aladaghense indicated that high antimicrobial activity on all bacteria than Galium incanum and Galium dieckii.

Download Full-text