In vivo and in vitro inhibitory effects of a traditional Chinese formulation on LPS-stimulated leukocyte–endothelial cell adhesion and VCAM-1 gene expression

2012 ◽  
Vol 140 (1) ◽  
pp. 55-63 ◽  
Author(s):  
Yi-Hong Wu ◽  
Shih-Yi Chuang ◽  
Wei-Chin Hong ◽  
Ying-Ju Lai ◽  
Ying-Ling Chang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Adhesion ◽  
Endothelial Cell ◽  
Inhibitory Effects ◽  
Endothelial Cell Adhesion

scholarly journals Soluble Domain 1 of Platelet–Endothelial Cell Adhesion Molecule (PECAM) Is Sufficient to Block Transendothelial Migration In Vitro and In Vivo

1997 ◽  
Vol 185 (7) ◽  
pp. 1349-1358 ◽  
Author(s):  
Fang Liao ◽  
Jahanara Ali ◽  
Tricia Greene ◽  
William A. Muller
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Transendothelial Migration ◽  
Endothelial Cell Adhesion Molecule ◽  
Platelet Endothelial Cell Adhesion ◽  
Endothelial Cell Adhesion

The inflammatory response involves sequential adhesive interactions between cell adhesion molecules of leukocytes and the endothelium. Unlike the several adhesive steps that precede it, transendothelial migration (diapedesis), the step in which leukocytes migrate between apposed endothelial cells, appears to involve primarily one adhesion molecule, platelet–endothelial cell adhesion molecule (PECAM, CD31). Therefore, we have focused on PECAM as a target for antiinflammatory therapy. We demonstrate that soluble chimeras made of the entire extracellular portion of PECAM, or of only the first immunoglobulin domain of PECAM, fused to the Fc portion of IgG, block diapedesis in vitro and in vivo. Furthermore, the truncated form of the PECAM-IgG chimera does not bind stably to its cellular ligand. This raises the possibility of selective anti-PECAM therapies that would not have the untoward opsonic or cell-activating properties of antibodies directed against PECAM.

Cholesterol-Modified Polyurethane Valve Cusps Demonstrate Blood Outgrowth Endothelial Cell Adhesion Post-Seeding In Vitro and In Vivo

2006 ◽  
Vol 81 (1) ◽  
pp. 47-55 ◽  
Author(s):  
Stanley J. Stachelek ◽  
Ivan Alferiev ◽  
Jeanne M. Connolly ◽  
Michael Sacks ◽  
Robert P. Hebbel ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Endothelial Cell Adhesion

Osteoprotegerin increases leukocyte adhesion to endothelial cells both in vitro and in vivo

Blood ◽  
2007 ◽  
Vol 110 (2) ◽  
pp. 536-543 ◽  
Author(s):  
Giorgio Zauli ◽  
Federica Corallini ◽  
Fleur Bossi ◽  
Fabio Fischetti ◽  
Paolo Durigutto ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Adhesion ◽  
Endothelial Cell ◽  
Leukocyte Adhesion ◽  
Polymorphonuclear Neutrophils ◽  
Heparin Binding ◽  
Hl60 Cells ◽  
Endothelial Cell Adhesion

AbstractRecombinant osteoprotegerin (OPG) promoted the adhesion of both primary polymorphonuclear neutrophils (PMNs) and leukemic HL60 cells to endothelial cells. Leukocyte/endothelial cell adhesion was promoted by short (peak at 1 hour) preincubation of either endothelial cells or PMNs with OPG, and the peak of proadhesive activity was observed in the same range of OPG concentrations detected in the sera of patients affected by cardiovascular diseases. Although the cognate high-affinity ligands for OPG, membrane receptor activator of nuclear factor-κB ligand (RANKL) and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), were detected at significant levels on both PMNs and HL60 cells, they were not expressed on the surface of endothelial cells. However, preincubation of OPG with heparin abrogated its proadhesive activity, whereas pretreatment of endothelial cells with chondroitinase plus heparinases significantly decreased the proadhesive activity of OPG. Taken together, these findings suggest the involvement of both the ligand binding and the N-terminal heparin-binding domains of OPG in mediating its pro-adhesive activity. The relevance of these in vitro findings was underscored by in vivo experiments, in which the topical administration of recombinant OPG increased leukocyte rolling and adhesion to rat mesenteric postcapillary venules. Our data suggest that a pathological increase of OPG serum levels might play an important role in promoting leukocyte/endothelial cell adhesion.

Enhanced Vascular Endothelial Cell Function on Nanostructured Titanium Surface Features: The Role of Nano to Submicron Roughness

2008 ◽  
Vol 1136 ◽  
Author(s):  
Jing Lu ◽  
Dongwoo Khang ◽  
Thomas J. Webster
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Cell Function ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Endothelial Cell Function ◽  
Titanium Surfaces ◽  
Endothelial Cell Adhesion

ABSTRACTTo study the contribution of different surface feature properties in improving vascular endothelial cell adhesion, rationally designed nano/sub-micron patterns with various dimensions were created on titanium surfaces in this study. In vitro results indicated that endothelial cell adhesion was improved when the titanium pattern dimensions decreased into the nano-scale. Specifically, endothelial cells preferred to adhere on sub-micron and nano rough titanium substrates compared to flat titanium. Moreover, titanium with nano and sub-micron roughness and with the same chemistry as compared to flat titanium, had significantly greater surface energy. Thus, the present study indicated the strong potential of surface nanotopography and nano/sub-micron roughness for improving current vascular stent design.

The anti-ischemic drugs defibrotide and oligotide analogously inhibit leukocyte-endothelial cell adhesion in vitro

1996 ◽  
pp. 420-424
Author(s):  
F. Pellegatta ◽  
E. Ferrero ◽  
A. Marni ◽  
S. Chierchia ◽  
D. Forti ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Endothelial Cell ◽  
Endothelial Cell Adhesion
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