Evaluation of the wound healing activity of methanol extract of Pedilanthus tithymaloides (L.) Poit leaf and its isolated active constituents in topical formulation

2012 ◽  
Vol 142 (3) ◽  
pp. 714-722 ◽  
Author(s):  
Soma Ghosh ◽  
Amalesh Samanta ◽  
Nirup Bikash Mandal ◽  
Sukdeb Bannerjee ◽  
Debprasad Chattopadhyay
Keyword(s):  
Wound Healing ◽  
Methanol Extract ◽  
Topical Formulation ◽  
Active Constituents ◽  
Wound Healing Activity

scholarly journals Studies on Wound Healing Activity of Heliotropium indicum Linn. Leaves on Rats

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
G. K. Dash ◽  
P. N. Murthy
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Dead Space ◽  
Methanol Extract ◽  
Infection Model ◽  
Aqueous Extracts ◽  
Reference Standard ◽  
Wound Model ◽  
Heliotropium Indicum ◽  
Wound Healing Activity

The petroleum ether, chloroform, methanol, and aqueous extracts of Heliotropium indicum Linn. (Family: Boraginaceae) were separately evaluated for their wound healing activity in rats using excision (normal and infected), incision, and dead space wound models. The effects of test samples on the rate of wound healing were assessed by the rate of wound closure, period of epithelialisation, wound breaking strength, weights of the granulation tissue, determination of hydroxyproline, super oxide dismutase (SOD), catalase, and histopathology of the granulation tissues. Nitrofurazone (0.2% w/w) in simple ointment I. P. was used as reference standard for the activity comparison. The results revealed significant promotion of wound healing with both methanol and aqueous extracts with more promising activity with the methanol extract compared to other extracts under study. In the wound infection model (with S. aureus and P. aeruginosa), the methanol extract showed significant healing activity similar to the reference standard nitrofurazone. Significant increase in the granulation tissue weight, increased hydroxyproline content, and increased activity of SOD and catalase level with the animals treated with methanol extract in dead space wound model further augmented the wound healing potential of H. indicum. The present work substantiates its validity of the folklore use.

Evaluation of the wound healing activity of Shorea robusta, an Indian ethnomedicine, and its isolated constituent(s) in topical formulation

2013 ◽  
Vol 149 (1) ◽  
pp. 335-343 ◽  
Author(s):  
Hemanta Mukherjee ◽  
Durbadal Ojha ◽  
Yogesh P. Bharitkar ◽  
Soma Ghosh ◽  
Supriya Mondal ◽  
...  
Keyword(s):  
Wound Healing ◽  
Topical Formulation ◽  
Shorea Robusta ◽  
Wound Healing Activity

Preliminary Phytochemical Analysis and Wound Healing Activity from the Leaves of Cocculus hirsutus Diels

2009 ◽  
Vol 2 (3) ◽  
pp. 675-678 ◽  
Author(s):  
Panda Bikash Ranjan ◽  
Mohanta S.R ◽  
Sii S. ◽  
Das G. K.
Keyword(s):  
Wound Healing ◽  
Petroleum Ether ◽  
Methanol Extract ◽  
Traditional Healers ◽  
Phytochemical Analysis ◽  
Plant Metabolites ◽  
Secondary Plant Metabolites ◽  
Scientific Support ◽  
Cocculus Hirsutus ◽  
Wound Healing Activity

In qualitative phytochemical analysis of petroleum ether, chloroform and methanol extracts of the leaves of Cocculus hirsutus Diels, the important secondary plant metabolites detected are Carbohydrates, Steroids, Glycosides, Tannins, Saponins and Flavonoids. The methanol extract and fresh leaves juice are subjected to wound healing activity in incision and excision wound models taking Soframycin sulphate as standard. The results revealed that the methanol extract ointment 10%w/w has highest wound healing potency among the test groups and is comparable with the standard and thus the results offer a scientific support to the treatment of traditional healers of this region.

scholarly journals Phytochemical Profile, Safety Assessment and Wound Healing Activity of Artemisia absinthium L.

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1744
Author(s):  
Amel Boudjelal ◽  
Antonella Smeriglio ◽  
Giovanna Ginestra ◽  
Marcella Denaro ◽  
Domenico Trombetta
Keyword(s):  
Wound Healing ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
In Vivo Studies ◽  
Oral Toxicity ◽  
Artemisia Absinthium ◽  
Traditional Use ◽  
Reference Drug ◽  
Wound Healing Activity

The aim of study was to validate, by in vitro and in vivo studies, the traditional use for wound-healing activity of Artemisia absinthium L. Reversed-phase liquid chromatography coupled with diode array detection and electrospray ion trap mass spectrometry (RP-LC-DAD-ESI-MS) analysis allowed to identify eleven polyphenols with chlorogenic acid as the most abundant compound (3.75 g/100 g of dry extract). After that, antibacterial activity as well as acute dermal and oral toxicity were assessed in animal models. In order to investigate the wound-healing activity of A. absinthium methanol extract, two ointments were formulated (MEO 5% and 10%). The ointment with the highest concentration of plant extract (10%) showed a statistically significant effect on the rats wound contraction, similar to that exerted by the reference drug Cicatryl-Bio. Moreover, A. absinthium methanol extract showed the best antibacterial activity against the Gram-negative Escherichia coli ATCC 10536 (MIC 1.25–2.5 mg/mL) and the Gram-positive Staphylococcus aureus ATCC 6538 (0.31–0.625 mg/mL). The absence of oral and topical toxicity of the treated animals allowed to establish the safety of the ointments. Overall, data collected in the present study support and validate the use of A. absinthium as a wound healing agent in the Algerian traditional medicine.

scholarly journals In Vivo Evaluation of Wound Healing and Anti-Inflammatory Activity of 80% Methanol Crude Flower Extract of Hagenia abyssinica (Bruce) J.F. Gmel in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-12 ◽  
Author(s):  
Teshome Fentik Belachew ◽  
Seyfe Asrade ◽  
Mestayt Geta ◽  
Engidaw Fentahun
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Methanol Extract ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Flower Extract ◽  
Hagenia Abyssinica ◽  
Anti Inflammatory ◽  
Wound Healing Activity

Background. Hagenia abyssinica (Bruce) J.F. Gmel (Rosaceae) is distributed in the highlands of Ethiopia. The flowers of Hagenia abyssinica (Bruce) J.F. Gmel are used traditionally to treat wound. However, there was no scientific report on wound healing activity of Hagenia abyssinica (Bruce) J.F. Gmel. Thus, this study was initiated to investigate the wound healing and anti-inflammatory activities of 80% methanol crude extract of flowers of Hagenia abyssinica in mice. Objective. The objective of this study was to evaluate the wound healing and anti-inflammatory activity of 80% methanol crude flower extract of Hagenia abyssinica in mice. Methods. Air-dried flowers of Hagenia abyssinica were grounded and macerated three times successively by 80% methanol. The dried extract was fractionated with chloroform, ethyl acetate, and water. Phytochemical screening tests were performed according to established procedures. The crude extract and solvent fractions were formulated as ointments. Wound healing activity of the crude extract was evaluated using excision and incision wound models, and the wound healing activities of solvent fractions were evaluated by using the excision wound model. The anti-inflammatory activity of the 80% methanol extract of Hagenia abyssinica was evaluated using carrageenan-induced hind paw edema model in mice. Result. The 2000 mg/kg test dose of the 10% (w/w) crude extract ointment was safe in rats. Both the 5% (w/w) and 10% (w/w) crude extract ointment-treated groups showed significant wound contraction starting from the day 4th. Both 5% (w/w) and 10% (w/w) crude extract ointments showed significant (P<0.001) increment of tensile strength compared to the negative control. The 10% (w/w) aqueous and ethyl acetate fraction ointment revealed high (P<0.001) percentage of wound contraction. The 100 mg/kg, 200 mg/kg, and 400 mg/kg oral administration of the crude extract had significant inhibition of the paw edema in mice of carrageenan-induced inflammation. Conclusion. The results of this study evidenced that both 5% w/w and 10% w/w 80% methanol extract ointment of the flowers of Hagenia abyssinica have wound healing and anti-inflammatory effects.

IN VIVO EVALUATION OF WOUND HEALING AND ANTI-INFLAMMATORY ACTIVITY OF METHANOLIC EXTRACT OF ROOTS OF CENTAUREA AFRICANA (L.) IN TOPICAL FORMULATION

2016 ◽  
Vol 10 (1) ◽  
pp. 341 ◽  
Author(s):  
Mohamed Zaouani ◽  
Arezki Bitam ◽  
Ahcen Baz ◽  
Yasmine Benali ◽  
Meriem Hind Ben-mahdi
Keyword(s):  
Wound Healing ◽  
Methanolic Extract ◽  
Wound Contraction ◽  
Inflammatory Activity ◽  
Hydroxyproline Content ◽  
Topical Formulation ◽  
In Vivo Evaluation ◽  
Anti Inflammatory ◽  
Wound Healing Activity

ABSTRACTObjective: The present study was to evaluate the anti-inflammatory and wound healing activities of methanolic extract of Centaurea africana rootsin albino Wistar rats.Methods: Following extraction of the C. africana roots with 80% methanol, the extract was formulated as an ointment (5% and 10% w/w). The ointmentwas then evaluated for wound healing activity using excision and incision wound models. Parameters, including wound contraction, epithelizationtime, histopathologically, and hydroxyproline content, were determined using the excision model, whereas tensile strength was measured from theincision model. In parallel, edema of the ear, locally induced by croton oil, was studied for the assessment of anti-inflammatory activity.Results: Wound treated with 5% and 10% methanolic extract ointment exhibited a significant wound healing activity in both models as evidencedby increased wound contraction, shorter epithelization time, higher tissue breaking strength, and increased hydroxyproline content. The methanolicextract also produced dose-related significant reduction (p<0.001) of inflammation.Conclusion: Results of the present study revealed that C. africana displays remarkable wound healing and anti-inflammatory activities.Keywords: Anti-inflammatory activity, Centaurea africana roots, Methanolic extract, Incision and excision wound model.

scholarly journals Wound Healing Potential of Crocin and Safranal, Main Saffron (Crocus sativus L.), the Active Constituents in Excision Wound Model in Rats

2021 ◽  
Vol 10 ◽  
Author(s):  
Nafise Deldar ◽  
Malihezaman Monsefi ◽  
Mohsen Salmanpour ◽  
Mohadeseh Ostovar ◽  
Mojtaba Heydari
Keyword(s):  
Wound Healing ◽  
Crocus Sativus ◽  
Control Group ◽  
Topical Formulation ◽  
Adult Rats ◽  
Wound Area ◽  
Active Constituents ◽  
Healing Effect ◽  
Wound Model ◽  
Wound Healing Effect

Background: Saffron is traditionally suggested for wound healing in Persian medicine. It is investigated for wound healing effect in multiple studies with promising results. It is not examined that which ingredient of saffron contributes more to this effect. This study was aimed to evaluate and compare the wound healing potential of saffron and its active constituents, crocin, and safranal, in rats. Materials and Methods: Forty female adult rats with induced excision wounds were randomly divided into four groups to receive topical formulation of saffron, crocin, safranal, and placebo for seven days. The Wound area and histopathologic stage of wound healing were evaluated as outcome measures. Results: The wound area was significantly lower in treatment groups (saffron, crocin, and safranal) compared to the control group on day 7 of the intervention. Compared to the control group, the wound in all treated groups showed a decreased inflammatory response and more progression to the proliferation phase. The saffron group showed more advanced healing phase with complete epithelialization of the wound on day 7 of study when partial and no epithelialization was observed in safranal and crocin groups. Conclusion: The study showed the wound healing properties of both safranal and crocin with the slight superiority of safranal. However, saffron seems to be more potent in wound healing effect compared to safranal and crocin as its main active constituents. [GMJ.2021;10:e1900]

Evaluation of in vivo wound healing activity of methanol extract of Achyranthes aspera L.

2012 ◽  
Vol 143 (2) ◽  
pp. 469-474 ◽  
Author(s):  
Abraham Fikru ◽  
Eyasu Makonnen ◽  
Tadesse Eguale ◽  
Asfaw Debella ◽  
Getinet Abie Mekonnen
Keyword(s):  
Wound Healing ◽  
Methanol Extract ◽  
Achyranthes Aspera ◽  
Wound Healing Activity
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