scholarly journals In Vivo Evaluation of Wound Healing and Anti-Inflammatory Activity of 80% Methanol Crude Flower Extract of Hagenia abyssinica (Bruce) J.F. Gmel in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-12 ◽  
Author(s):  
Teshome Fentik Belachew ◽  
Seyfe Asrade ◽  
Mestayt Geta ◽  
Engidaw Fentahun
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Methanol Extract ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Flower Extract ◽  
Hagenia Abyssinica ◽  
Anti Inflammatory ◽  
Wound Healing Activity

Background. Hagenia abyssinica (Bruce) J.F. Gmel (Rosaceae) is distributed in the highlands of Ethiopia. The flowers of Hagenia abyssinica (Bruce) J.F. Gmel are used traditionally to treat wound. However, there was no scientific report on wound healing activity of Hagenia abyssinica (Bruce) J.F. Gmel. Thus, this study was initiated to investigate the wound healing and anti-inflammatory activities of 80% methanol crude extract of flowers of Hagenia abyssinica in mice. Objective. The objective of this study was to evaluate the wound healing and anti-inflammatory activity of 80% methanol crude flower extract of Hagenia abyssinica in mice. Methods. Air-dried flowers of Hagenia abyssinica were grounded and macerated three times successively by 80% methanol. The dried extract was fractionated with chloroform, ethyl acetate, and water. Phytochemical screening tests were performed according to established procedures. The crude extract and solvent fractions were formulated as ointments. Wound healing activity of the crude extract was evaluated using excision and incision wound models, and the wound healing activities of solvent fractions were evaluated by using the excision wound model. The anti-inflammatory activity of the 80% methanol extract of Hagenia abyssinica was evaluated using carrageenan-induced hind paw edema model in mice. Result. The 2000 mg/kg test dose of the 10% (w/w) crude extract ointment was safe in rats. Both the 5% (w/w) and 10% (w/w) crude extract ointment-treated groups showed significant wound contraction starting from the day 4th. Both 5% (w/w) and 10% (w/w) crude extract ointments showed significant (P<0.001) increment of tensile strength compared to the negative control. The 10% (w/w) aqueous and ethyl acetate fraction ointment revealed high (P<0.001) percentage of wound contraction. The 100 mg/kg, 200 mg/kg, and 400 mg/kg oral administration of the crude extract had significant inhibition of the paw edema in mice of carrageenan-induced inflammation. Conclusion. The results of this study evidenced that both 5% w/w and 10% w/w 80% methanol extract ointment of the flowers of Hagenia abyssinica have wound healing and anti-inflammatory effects.

scholarly journals Anti-Inflammatory Activity of Chrozophora rottleri Extracts on Carrageenan-Induced Rat Paw Edema

2016 ◽  
Vol 3 ◽  
pp. 20-26
Author(s):  
Mallikarjuna Rao Talluri ◽  
Battu Ganga Rao ◽  
Y. Venkateswaea Rao
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Bioactive Molecules ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Standard Drug ◽  
Isolation And Characterization ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw

The present study was intended to evaluate Anti-inflammatory activity ofC. rottleriextracts (Hydroalcoholic, Methanol, Ethyl acetate and Hexane). The Anti-inflammatory activity ofC.rottleriextracts at doses of 125mg/kg, 250mg/kg and 500mg/kg using carrageenan induced rat paw edema model compared with standard drug (Indomethacin). The selected plant extracts significantly inhibited paw edema along with the standard drug Indomethacin. Of all extracts, methanol extract produced significant effect on reduction of increased paw thickness, hydro alcoholic and ethyl acetate extracts produced moderate percentage inhibition and hexane extract produced low level of percentage inhibition in reducing paw edema on carrageenan induced rats. In all extracts, methanol extract at a dose of 500mg/kg showed more percentage inhibition i.e . 53.47±2.19. From the results obtained during the study it is concluded thatC. rottlerihaving the bioactive molecule responsible for Anti-inflammatory activity by individually or by combination of different bio-active compounds present in it. Further is necessary for isolation and characterization of bioactive molecules which are responsible for the selected plant biological activities.

scholarly journals Anti-Inflammatory Activity and Composition ofSenecio salignusKunth

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Cuauhtemoc Pérez González ◽  
Roberto Serrano Vega ◽  
Marco González-Chávez ◽  
Miguel Angel Zavala Sánchez ◽  
Salud Pérez Gutiérrez
Keyword(s):  
Medicinal Plant ◽  
Methanol Extract ◽  
Chloroform Extract ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Active Fraction ◽  
Ear Edema ◽  
Methanol Extracts ◽  
Anti Inflammatory ◽  
Inflammatory Effect

We investigated the anti-inflammatory activity ofSenecio salignus. This medicinal plant is often used in Mexico for the treatment of fever and rheumatism. Chloroform and methanol extracts of the plant were tested on 12-O-tetradecanoylphorbol-13-acetate- (TPA-) induced edema in mice ears. The methanol extract of the plant inhibited edema by36±4.4% compared with the control, while the chloroform extract exhibited an even greater level of inhibition (64.1%). The chloroform extract was then fractionated, and the composition of the active fraction was determined by GC-MS. The anti-inflammatory activity of this fraction was then tested on TPA-induced ear edema in mice, and we found that the active fraction could inhibit edema by 46.9%. The anti-inflammatory effect of the fraction was also tested on carrageenan-induced paw edema in rats at doses of 100 mg/kg; a58.9±2.8% reduction of the edema was observed 4 h after administration of carrageenan, and the effect was maintained for 5 h.

scholarly journals PHYTOCHEMICAL PROFILING, FREE-RADICAL SCAVENGING AND ANTI-INFLAMMATORY ACTIVITIES OF MELIOSMA SIMPLICIFOLIA (L.)

2020 ◽  
pp. 62-68
Author(s):  
S. PAVITHR ◽  
T. SEKAR
Keyword(s):  
Free Radical ◽  
Methanol Extract ◽  
Inflammatory Activity ◽  
Scavenging Activity ◽  
Paw Edema ◽  
Stem Extract ◽  
Ft Ir ◽  
Ir Analysis ◽  
Anti Inflammatory ◽  
Dpph Scavenging

Objective: In the current research, to determine the stem extract of Meliosma simplicifolia (L.) for total phenol, tannin, total flavonoid, antioxidant activity, anti-inflammatory and identify the phytoconstituents utilizing GC-MS and FT-IR. Methods: The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, superoxide radical (O2•) scavenging activity. The anti-inflammatory activity was evaluated using carrageenan and formalin-induced paw edema models using Wistar albino rats. The GC-MS and FT-IR analysis of the methanolic stem extract of M. simplicifolia was revealed the presence of phytochemicals. Results: Quantitative studies of estimated phenol, flavonoid and tannin, as for the methanol extract of stem showed the highest content of phenolic compounds (39.83±3.62GAE mg/100). Antioxidant activities were concluded the estimation M. simplicifolia stem for as followed the studies. In stem the methanol extract showed the highest DPPH scavenging activity (124.3µg/ml). The anti-inflammatory activity has shown in high doses of methanolic extract 250 mg/kg of significant value (p<0.05) inhibition of paw edema, on 6th hour, respectively. The FT-IR analysis has confirmed their characteristic peak values and functional groups. Conclusion: M. simplicifolia has an effective of anti-inflammatory activity and constitutes a potential source for the development of new treatments.

The Anti-inflammatory Activity of Scutellaria rivularis Extracts and Its Active Components, Baicalin, Baicalein and Wogonin

1996 ◽  
Vol 24 (01) ◽  
pp. 31-36 ◽  
Author(s):  
Chun-Ching Lin ◽  
Den-En Shieh
Keyword(s):  
Ethyl Acetate ◽  
Chloroform Extract ◽  
Inflammatory Activity ◽  
Inhibition Activity ◽  
Paw Edema ◽  
Active Components ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw

Five extracts (n-hexane, chloroform, ethyl acetate, n-butanol and water) of Scutellaria rivularis Benth. were evaluated for their anti-inflammatory activity against carrageenan-induced paw edema in rats and compared with indomethacin. The result indicated that chloroform extract proved to be the most effective in all of the extracts. Consequently, three major components (baicalin, baicalein and wogonin) of the chloroform extract were further tested for their anti-inflammatory activity using the same model. It was found that baicalin exhibits the greatest inhibition activity against carrageenan-induced rat paw edema.

scholarly journals Evaluation of anti-inflammatory activity of Jasminum sessiliflorum extracts

2019 ◽  
Vol 10 (3) ◽  
pp. 2515-2518
Author(s):  
Ruby Philip ◽  
Kathiresan Krishnasamy ◽  
Elessy Abraham
Keyword(s):  
Medicinal Plants ◽  
Adverse Effects ◽  
Ethyl Acetate ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Reference Drug ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Dose Dependent ◽  
Ethyl Acetate Extracts

Medicinal plants are important resources for the development of novel anti-inflammatory agents. Synthetic anti-inflammatory drugs have several adverse effects. Medicinal plants have numerous that are safer well as better substitutes for the prevention and management of various diseases and disorders. The and ethyl acetate extracts of Jasminum were evaluated for their potential as anti-inflammatory agents in by inducing paw edema in rats using . The anti-inflammatory activity of the reference drug was compared with the extracts of Jasminum . The extracts on screening indicated the presence of such as , , , alkaloids, and . The study established significant anti-inflammatory nature of the Jasminum extracts in a manner which is dose-dependent. The results indicate that both the extracts of Jasminum possess anti-inflammatory activity of significance and can be used in the development for novel anti-inflammatory moieties.

Isolation of a stimulatory system in an Aloe extract

1991 ◽  
Vol 81 (9) ◽  
pp. 473-478 ◽  
Author(s):  
RH Davis ◽  
WL Parker ◽  
RT Samson ◽  
DP Murdoch
Keyword(s):  
Wound Healing ◽  
Lower Extremity ◽  
Aloe Vera ◽  
Ethanol Extract ◽  
Inflammatory Activity ◽  
Supernatant Fraction ◽  
Croton Oil ◽  
Precipitate Fraction ◽  
Anti Inflammatory ◽  
Wound Healing Activity

The authors' previous work on a 50% ethanol extract of Aloe vera was done to evaluate anti-inflammatory activity using the croton oil-induced ear swelling assay. The anti-inflammatory activity was found in the supernatant fraction. The supernatant fraction decreased inflammation, when applied topically, by 29.2%, and the precipitate decreased inflammation by 12.1%. However, in the present work, the precipitate fraction decreased the wound diameter by an average of 47.1% (stimulatory system). Little or no wound healing activity was found in the supernatant. Aloe vera appears to act as a modulatory system toward wounds and inflammation and is a potentially valuable tool for managing lower extremity conditions.

scholarly journals Anti-Inflammatory Activity of Salvadora persica L. against Carrageenan Induced Paw Oedema in Rat Relevant to Inflammatory Cytokines

2011 ◽  
Vol 3 (4) ◽  
pp. 22-28 ◽  
Author(s):  
Abeer Y. IBRAHIM ◽  
Souad E. El-GENGAIHI ◽  
Hemaia M. MOTAWEA ◽  
Amany A. SLEEM
Keyword(s):  
Ethyl Acetate ◽  
Inflammatory Cytokines ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
Inflammatory Activity ◽  
Alcoholic Extract ◽  
Salvadora Persica ◽  
Group I ◽  
Paw Oedema ◽  
Anti Inflammatory

In this study, the anti-inflammatory effect of aqueous alcoholic crude extract and ethyl acetate extract of miswak sticks (Salvadora persica L.) was investigated in carrageenan induced rat paw oedema in respect to immunological parameters. Adult male sapargue dawely rats were classified into four groups, group I received the vehicle (0.25% gum acacia solution), group II received crude aqueous alcoholic extract orally at 100 mg/kg, group III received ethyl acetate extract (100 mg/kg) orally and group IV received indomethacin (20 mg/kg) orally, and served as standard reference. The oedema was quantified by measuring the hind paw thickness immediately before subplantar injection, and at 1, 2, 3 and 4 h. Blood samples were withdrawn after the 4th hour of carrageenan induction, centrifuged and sera were used for analysis of pro-inflammatory cytokines. Administration of aqueous alcoholic extract and ethyl acetate extract (100 mg/ml) significantly reduced the oedema thickness in a time dependent manner, the inhibition percentage of inflammation was 17% for crude extract and 27% for ethyl acetate extract. Also the two extracts reduced secretion of inflammatory mediators, interleukin-1β (IL-1β), IL-6, tumor nercrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) in serum. The ethyl acetate extract shows potent activity to be nearly the same of indomethacin activity on all determined parameters at the last hour of following up. These results may be due to the presence of flavonoids in ethyl acetate extract. Three major flavonoids were isolated from ethyl acetate extract and identified as apigenin rhamnoglucoside, luteolin glucoside and rutin. The experimental study revealed that Salvadora persica extracts display remarkable anti-inflammatory activity.

scholarly journals PHYTOCHEMICAL SCREENING AND ANTI-INFLAMMATORY ACTIVITY OF FRACTIONS FROM SAMBUNG RAMBAT (MICANIA CORDATA) LEAF

2018 ◽  
Vol 11 (8) ◽  
pp. 199
Author(s):  
Zola Efa Harnis ◽  
Panal Sitorus ◽  
Rosidah Rosidah
Keyword(s):  
Ethyl Acetate ◽  
Screening Method ◽  
Ethyl Acetate Fraction ◽  
Inflammatory Activity ◽  
Phytochemical Screening ◽  
Paw Edema ◽  
Water Solvent ◽  
Phytochemical Compounds ◽  
Anti Inflammatory

Objective: The present study is to evaluate the anti-inflammatory activity of various fractions from Sambung Rambat leaf (Micania cordata) using ethyl acetate, hexane, and water solvent as the solvent.Methods: Investigation of phytochemical compound was using standard phytochemical screening method, while the anti-inflammatory was using the carrageenan-induced paw edema method in animal (rats) model.Results: The phytochemical screening showed that the fractions of M. cordata had a lot of phytochemical compounds such as flavonoids, glycosides, steroids, triterpenoids, tannins, and saponins. Anti-inflammatory assessment showed that the strongest activity of anti-inflammatory produced by ethyl acetate fraction with 200 mg/kg BW dose.Conclusion: This investigation could be concluded that ethyl acetate fraction of M. cordata might be a potential for the treatment of inflammatory.

scholarly journals Anti-inflammatory activity of a methanol extract from Ardisia tinctoria on mouse macrophages and paw edema

2014 ◽  
Vol 9 (4) ◽  
pp. 1388-1394 ◽  
Author(s):  
HUI-SEONG KIM ◽  
JI-WON PARK ◽  
OK-KYOUNG KWON ◽  
JEA-HONG KIM ◽  
SEI-RYANG OH ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Mouse Macrophages ◽  
Anti Inflammatory
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