Evaluation of anticancer activities of Poria cocos ethanol extract in breast cancer: In vivo and in vitro, identification and mechanism

Aim. The present study aimed to isolate flavonoid fraction from the aerial parts ofCissus quadrangularisand to evaluate its antioxidant and anticancer potential usingin vitroassay system.Methods. Total phenolic and flavonoid contents were calculated for the drug. Flavonoid fraction was isolated using column chromatography and analysed using HPLC.In vitro, antioxidant activity of the ethanol extract and isolated flavonoid fraction was investigated by nitric oxide, DPPH and hydroxyl radical scavenging assays. Breast cancer (MCF 7) cell line was used as thein vitrocancer model for MTT assay.Result. The amount of total phenolic content and total flavonoid content in the ethanol extract showed 28.6 mg/g dry weight expressed as gallic acid equivalents, and 15.8 mg/g was expressed as quercetin equivalents, respectively. The tested extract showed good dose-dependent free radical scavenging property in all the models with the IC50values of 98 μg/mL, 125 μg/mL, and 96 μg/mL for ethanol extract and 10 μg/mL, 12 μg/mL, and 10 μg/mL for flavonoid fraction, respectively. The flavonoid fraction possess potent anticancer property against breast cancer cells (MCF7) with IC50value of 40 μg/mL.Conclusions. It can be concluded that the aerial part ofCissus quadrangularishas potential antioxidant and anticancer activities.

Download Full-text

Anti-EGFR anchored paclitaxel loaded PLGA nanoparticles for the treatment of triple negative breast cancer. In-vitro and in-vivo anticancer activities

PLoS ONE ◽

10.1371/journal.pone.0206109 ◽

2018 ◽

Vol 13 (11) ◽

pp. e0206109 ◽

Cited By ~ 13

Author(s):

Vijayan Venugopal ◽

Shalini Krishnan ◽

Vasanth Raj Palanimuthu ◽

Subin Sankarankutty ◽

Jayaraja Kumar Kalaimani ◽

...

Keyword(s):

Breast Cancer ◽

Triple Negative Breast Cancer ◽

Triple Negative ◽

Plga Nanoparticles ◽

Anticancer Activities

Download Full-text

Anticancer Activities of an Anthocyanin-Rich Extract From Black Rice Against Breast Cancer Cells In Vitro and In Vivo

Nutrition and Cancer ◽

10.1080/01635581.2010.494821 ◽

2010 ◽

Vol 62 (8) ◽

pp. 1128-1136 ◽

Cited By ~ 93

Author(s):

Chang Hui ◽

Yu Bin ◽

Yu Xiaoping ◽

Yi Long ◽

Chen Chunye ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Black Rice ◽

Anticancer Activities

Download Full-text

Aktivitas Sitotoksik Fraksi Alkaloid Kulit Batang dan Buah Attarasa (Litsea cubeba Lour.) terhadap Sel Kanker Payudara T47D

Talenta Conference Series Tropical Medicine (TM) ◽

10.32734/tm.v1i3.261 ◽

2018 ◽

Vol 1 (3) ◽

pp. 052-055

Author(s):

Aminah Dalimunthe ◽

Poppy Anjelisa Zaitun Hasibuan ◽

Jansen Silalahi ◽

Denny Satria

Keyword(s):

Breast Cancer ◽

Cytotoxic Activity ◽

In Vitro Study ◽

Ethanol Extract ◽

T47d Cell ◽

Chloroform Fraction ◽

Anticancer Activities ◽

Litsea Cubeba ◽

Alkaloid Fraction

Kanker payudara adalah salah satu jenis dari kanker yang banyak menyerang wanita selain kanker mulut rahim. Tingginya angka kejadian dan resistensi agen kemoterapi menyebabkan perlu dilakukan pencarian bahan alam dengan aktivitas antikanker. Penggunaan bahan alam diharapkan dapat mengatasi resistensi dan efek samping yang terjadi ketika digunakan kemoterapi konvensional. Tujuan penelitian ini untuk mengetahui aktivitas antikanker fraksi kloroform (alkaloid) kulit batang dan buah attarasa pada sel T47D. Ekstrak etanol diekstraksi dengan cara maserasi dan difraksinasi dengan n-heksana dan kloroform pada pH 3, 7, 9. Pengujian sitotoksik secara in vitro menggunakan metode MTT yang kemudian dianalisis menggunakan SPSS 21. Hasil uji sitotoksik (IC50) yang diperoleh setelah pemberian fraksi alkaloid kulit batang dan buah attarasa pH 7 dan 9 adalah sebesar 46,60 ± 0,19; 123,01 ± 14,63 dan 35,89 ± 1,04; dan 98,31 ± 2,51 µg/mL. Fraksi alkaloid kulit batang dan buah attarasa bersifat sitotoksik terhadap sel kanker payudara T47D. Breast cancer is one type of cancer that attacks many women in addition to cervical cancer. The high incidence and resistance of chemotherapy agents causes the need to search for natural products with anticancer activities. The use of natural product is expected to overcome resistance and side effects that occur when used conventional chemotherapy. This research aimed to evaluate the anti-cancer activity of chloroform fraction (alkaloid) from cortex and Fructus of Attarasa on T47D cell. The ethanol extract was prepared by maceration method and then fractionated using n-hexane and chloroform at pH 3, 7, and 9. In vitro study of cytotoxic activity was performed using MTT method, then analyzed using SPSS 21. The IC50 values of cytotoxic activity of alkaloid fraction from cortex and Fructus of Attarasa at pH 7 dan 9 were 46.60 ± 0.19; 123.01 ± 14.63 and 35.89 ± 1.04; dan 98.31 ± 2.51 µg/mL. Alkaloid fraction from cortex skin and Fructus of Attarasa has cytotoxic activity on Breast Cancer T47D Cell

Download Full-text

Extracellular cholesterol oxidase production by Streptomyces aegyptia, in vitro anticancer activities against rhabdomyosarcoma, breast cancer cell-lines and in vivo apoptosis

Scientific Reports ◽

10.1038/s41598-018-20786-3 ◽

2018 ◽

Vol 8 (1) ◽

Cited By ~ 10

Author(s):

Noura El-Ahmady El-Naggar ◽

Hoda M. Soliman ◽

Nancy M. El-Shweihy

Keyword(s):

Breast Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Cholesterol Oxidase ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Anticancer Activities

Download Full-text

Combination of a novel growth hormone releasing hormone Antagonist JMR 132 and Tamoxifen enhances the inhibitory effects on growth of ZR 75 and T47D estrogen dependent human breast cancer cells in vitro and in vivo

Geburtshilfe und Frauenheilkunde ◽

10.1055/s-0029-1225201 ◽

2009 ◽

Vol 69 (05) ◽

Author(s):

S Buchholz ◽

AV Schally ◽

A Krishan ◽

A Papadia ◽

O Ortmann ◽

...

Keyword(s):

Breast Cancer ◽

Growth Hormone ◽

Human Breast Cancer ◽

Breast Cancer Cells ◽

Growth Hormone Releasing Hormone ◽

Human Breast Cancer Cells ◽

Human Breast ◽

Inhibitory Effects

Download Full-text

Melatonin modifies tumor hypoxia and metabolism by inhibiting HIF-1α and energy metabolic pathway in the in vitro and in vivo models of breast cancer

Melatonin Research ◽

10.32794/mr11250042 ◽

2019 ◽

Vol 2 (4) ◽

pp. 83-98 ◽

Cited By ~ 2

Author(s):

André De Lima Mota ◽

Bruna Vitorasso Jardim-Perassi ◽

Tialfi Bergamin De Castro ◽

Jucimara Colombo ◽

Nathália Martins Sonehara ◽

...

Keyword(s):

Breast Cancer ◽

Glucose Metabolism ◽

Tumor Growth ◽

Tumor Cells ◽

Carbonic Anhydrases ◽

In Vivo Models ◽

Ca Ix ◽

Gene And Protein Expression

Breast cancer is the most common cancer among women and has a high mortality rate. Adverse conditions in the tumor microenvironment, such as hypoxia and acidosis, may exert selective pressure on the tumor, selecting subpopulations of tumor cells with advantages for survival in this environment. In this context, therapeutic agents that can modify these conditions, and consequently the intratumoral heterogeneity need to be explored. Melatonin, in addition to its physiological effects, exhibits important anti-tumor actions which may associate with modification of hypoxia and Warburg effect. In this study, we have evaluated the action of melatonin on tumor growth and tumor metabolism by different markers of hypoxia and glucose metabolism (HIF-1α, glucose transporters GLUT1 and GLUT3 and carbonic anhydrases CA-IX and CA-XII) in triple negative breast cancer model. In an in vitro study, gene and protein expressions of these markers were evaluated by quantitative real-time PCR and immunocytochemistry, respectively. The effects of melatonin were also tested in a MDA-MB-231 xenograft animal model. Results showed that melatonin treatment reduced the viability of MDA-MB-231 cells and tumor growth in Balb/c nude mice (p <0.05). The treatment significantly decreased HIF-1α gene and protein expression concomitantly with the expression of GLUT1, GLUT3, CA-IX and CA-XII (p <0.05). These results strongly suggest that melatonin down-regulates HIF-1α expression and regulates glucose metabolism in breast tumor cells, therefore, controlling hypoxia and tumor progression.

Download Full-text