scholarly journals Aktivitas Sitotoksik Fraksi Alkaloid Kulit Batang dan Buah Attarasa (Litsea cubeba Lour.) terhadap Sel Kanker Payudara T47D

2018 ◽  
Vol 1 (3) ◽  
pp. 052-055
Author(s):  
Aminah Dalimunthe ◽  
Poppy Anjelisa Zaitun Hasibuan ◽  
Jansen Silalahi ◽  
Denny Satria
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
In Vitro Study ◽  
Ethanol Extract ◽  
T47d Cell ◽  
Chloroform Fraction ◽  
Anticancer Activities ◽  
Litsea Cubeba ◽  
Alkaloid Fraction

Kanker payudara adalah salah satu jenis dari kanker yang banyak menyerang wanita selain kanker mulut rahim. Tingginya angka kejadian dan resistensi agen kemoterapi menyebabkan perlu dilakukan pencarian bahan alam dengan aktivitas antikanker. Penggunaan bahan alam diharapkan dapat mengatasi resistensi dan efek samping yang terjadi ketika digunakan kemoterapi konvensional. Tujuan penelitian ini untuk mengetahui aktivitas antikanker fraksi kloroform (alkaloid) kulit batang dan buah attarasa pada sel T47D. Ekstrak etanol diekstraksi dengan cara maserasi dan difraksinasi dengan n-heksana dan kloroform pada pH 3, 7, 9. Pengujian sitotoksik secara in vitro menggunakan metode MTT yang kemudian dianalisis menggunakan SPSS 21. Hasil uji sitotoksik (IC50) yang diperoleh setelah pemberian fraksi alkaloid kulit batang dan buah attarasa pH 7 dan 9 adalah sebesar 46,60 ± 0,19; 123,01 ± 14,63 dan 35,89 ± 1,04; dan 98,31 ± 2,51 µg/mL. Fraksi alkaloid kulit batang dan buah attarasa bersifat sitotoksik terhadap sel kanker payudara T47D.   Breast cancer is one type of cancer that attacks many women in addition to cervical cancer. The high incidence and resistance of chemotherapy agents causes the need to search for natural products  with anticancer activities. The use of natural product is expected to overcome resistance and side effects that occur when used conventional chemotherapy.  This research aimed to evaluate the anti-cancer activity of chloroform fraction (alkaloid) from cortex and Fructus of Attarasa on T47D cell. The ethanol extract was prepared by maceration method and then fractionated using n-hexane and chloroform at pH 3, 7, and 9. In vitro study of cytotoxic activity was performed using MTT method, then analyzed using  SPSS 21. The IC50 values of cytotoxic activity of alkaloid fraction from cortex and Fructus of Attarasa at pH 7 dan 9 were 46.60 ± 0.19; 123.01 ± 14.63 and 35.89 ± 1.04; dan 98.31 ± 2.51 µg/mL. Alkaloid fraction from cortex skin and Fructus of Attarasa has cytotoxic activity on Breast Cancer T47D Cell

scholarly journals In VitroAntioxidant and Anticancer Activity of Flavonoid Fraction from the Aerial Parts ofCissus quadrangularisLinn. against Human Breast Carcinoma Cell Lines

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
A. Vijayalakshmi ◽  
P. R. Kumar ◽  
S. Sakthi Priyadarsini ◽  
C. Meenaxshi
Keyword(s):  
Breast Cancer ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Dry Weight ◽  
Total Phenolic ◽  
Anticancer Activities ◽  
Aerial Parts ◽  
Cissus Quadrangularis ◽  
Flavonoid Fraction

Aim. The present study aimed to isolate flavonoid fraction from the aerial parts ofCissus quadrangularisand to evaluate its antioxidant and anticancer potential usingin vitroassay system.Methods. Total phenolic and flavonoid contents were calculated for the drug. Flavonoid fraction was isolated using column chromatography and analysed using HPLC.In vitro, antioxidant activity of the ethanol extract and isolated flavonoid fraction was investigated by nitric oxide, DPPH and hydroxyl radical scavenging assays. Breast cancer (MCF 7) cell line was used as thein vitrocancer model for MTT assay.Result. The amount of total phenolic content and total flavonoid content in the ethanol extract showed 28.6 mg/g dry weight expressed as gallic acid equivalents, and 15.8 mg/g was expressed as quercetin equivalents, respectively. The tested extract showed good dose-dependent free radical scavenging property in all the models with the IC50values of 98 μg/mL, 125 μg/mL, and 96 μg/mL for ethanol extract and 10 μg/mL, 12 μg/mL, and 10 μg/mL for flavonoid fraction, respectively. The flavonoid fraction possess potent anticancer property against breast cancer cells (MCF7) with IC50value of 40 μg/mL.Conclusions. It can be concluded that the aerial part ofCissus quadrangularishas potential antioxidant and anticancer activities.

scholarly journals Cytotoxic Activity of BornUSU I towards T47D Breast Cancer Cells

2019 ◽  
Vol 7 (22) ◽  
pp. 3816-3818
Author(s):  
Nadiya Anandita Nasution ◽  
Urip Harahap ◽  
Ginda Haro ◽  
Hari Purnomo ◽  
Denny Satria
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Incubation Period ◽  
Breast Cancer Cells ◽  
In Vitro Study ◽  
Positive Control ◽  
Breast Cancer Treatment ◽  
Mtt Method ◽  
Ic50 Value

AIM: The aim of this study was to determine cytotoxic activity of BornUSU I or Boronhafagama I (1,5-bis(4-hydroxyphenyl)-3-oxa-1,5-diaza-2,4-diboropentane-2,4-diol) as a boron derivate compounds which are boron neutron captured theraphy (BNCT) candidates. METHODS: The T47D cells were treated by BornUSU I, and Tamoxifen as a positive control. The in vitro study was using MTT method with the incubation period for 24h and 48h. All data were determined using viability of cells equation for showing each IC50 value. RESULTS: The IC50 value of BornUSU I and Tamoxifen were 72.61 ± 0.82 µM and 10.62 ± 0.06 µM for 24 h incubation period, and for the 48 h incubation period were 44.63 ± 0.23 µM and 7.79 ± 0.05 µM. The 48 h incubation period results showed the lowest IC50 value. CONCLUSION: The results reveal that BornUSU I provide effective as anticancer, especially for breast cancer treatment.

scholarly journals Bioguided Fractionation of Local Plants against Matrix Metalloproteinase9 and Its Cytotoxicity against Breast Cancer Cell Models: In Silico and In Vitro Study

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4691
Author(s):  
Maywan Hariono ◽  
Rollando Rollando ◽  
Jasson Karamoy ◽  
Pandu Hariyono ◽  
M. Atmono ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Crude Extract ◽  
In Silico ◽  
Metastatic Cancer ◽  
In Vitro Study ◽  
T47d Cell ◽  
Ageratum Conyzoides ◽  
Local Plants ◽  
Cancer Agent

Matrix metalloproteinase9 (MMP9) is known to be highly expressed during metastatic cancer where most known potential inhibitors failed in the clinical trials. This study aims to select local plants in our state, as anti-breast cancer agent with hemopexin-like domain of MMP9 (PEX9) as the selective protein target. In silico screening for PEX9 inhibitors was performed from our in house-natural compound database to identify the plants. The selected plants were extracted using methanol and then a step-by-step in vitro screening against MMP9 was performed from its crude extract, partitions until fractions using FRET-based assay. The partitions were obtained by performing liquid–liquid extraction on the methanol extract using n-hexane, ethylacetate, n-butanol, and water representing nonpolar to polar solvents. The fractions were made from the selected partition, which demonstrated the best inhibition percentage toward MMP9, using column chromatography. Of the 200 compounds screened, 20 compounds that scored the binding affinity −11.2 to −8.1 kcal/mol toward PEX9 were selected as top hits. The binding of these hits were thoroughly investigated and linked to the plants which they were reported to be isolated from. Six of the eight crude extracts demonstrated inhibition toward MMP9 with the IC50 24 to 823 µg/mL. The partitions (1 mg/mL) of Ageratum conyzoides aerial parts and Ixora coccinea leaves showed inhibition 94% and 96%, whereas their fractions showed IC50 43 and 116 µg/mL, respectively toward MMP9. Using MTT assay, the crude extract of Ageratum exhibited IC50 22 and 229 µg/mL against 4T1 and T47D cell proliferations, respectively with a high safety index concluding its potential anti-breast cancer from herbal.

scholarly journals Cytotoxic, Antiproliferation, and Necrosis Effects of the n-Hexane Fraction Extract of Gendola Leaf (Basella rubra Linn.)

2019 ◽  
Vol 23 (1) ◽  
pp. 8-13
Author(s):  
Sri Hartini Harianja ◽  
Salni Salni ◽  
Subandrate Subandrate
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Incubation Time ◽  
Breast Cancer Cells ◽  
Ethanol Extract ◽  
T47d Cell ◽  
Hexane Fraction ◽  
Laboratory Research ◽  
Concentration Series

Breast cancer is the second most common cancer in women. Several studies have been conducted on natural materials to see the ability of its activity as a new anticancer compound. The purpose of this study was to determine the cytotoxic activity of Gendola leaf (Basella rubra Linn.) extracts and fractions, in inhibiting proliferation and inducing apoptosis of T47D breast cancer cells. The design of this study was experimental laboratory research using the in-vitro method. Cytotoxic and anti-proliferation tests on T47D cancer were using the MTT method, and the apoptosis test was using the flow cytometry method. Cytotoxic of extracts and fractions of n-hexane Gendola leaves were tested with a concentration series of 1000; 500; 250; 125; 62.5 μg/mL and doxorubicin was studied a series concentration of 0.5; 0.25; 0.125; 0.0625; 0.03125 μg/mL for 24 hours. Antiproliferation test used n-hexane fraction with a concentration series of 1IC50, ½IC50, ¼IC50, and ⅛IC50 with an incubation time of 24 and 48 hours. Apoptosis test utilized n-hexane fraction with a concentration series of 1IC50, ½IC50 with an incubation time of 24 hours. The results showed that the ethanol extract had IC50 424 μg/mL, n-hexane fraction 292 μg/mL. The n-hexane fraction had an anti-proliferation effect at a concentration of 1IC50 within 32 hours but was unable to induce T47D cell apoptosis. These results indicate that the n-hexane fraction of Gendola leaf has a cytotoxic effect, which can inhibit proliferation, that is unable to induce apoptosis but induce necrosis of T47D breast cancer cells.

scholarly journals The Cytotoxic Activity of Annona muricata Linn Leaves Ethanolic Extract (AMEE) on T47D Breast Cancer Cell Line

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Soilia Fertilita ◽  
Willy Sandhika ◽  
Desak Gede Agung Suprabawati
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Ethanol Extract ◽  
T47d Cell ◽  
Annona Muricata ◽  
T47d Breast Cancer Cell ◽  
Viability Percentage

Breast cancer is the most common cancer in women throughout the world, with new cases and deaths which continue to increase. Soursop leaves (Annona muricata L) have been used extensively in traditional medicine, including cancer. Acetogenin, alkaloids, and phenols contained in soursop leaves are known to have anti-cancer effects. Among them, acetogenin has the most dominant role and reported to have a cytotoxic effect on various cancer cell lines. This study aims to determine the cytotoxic activity of soursop leaf ethanol extract on T47D breast cancer cell line. Measurement of cytotoxic activity was carried out by the MTT method, and the viability percentage of T47D cells was calculated based on the absorbance values in the treatment, cell control, and media control groups of each replicate. The correlation between extract concentration and viability percentage of the T47D cell line was outlined in the regression equation to obtain the IC50 value. IC50 values of 109.91 ± 3.04 with R values 0.975 and R2 0.9508 obtained. R values close to 1 indicated a strong correlation between extract concentration and the percentage of living T47D cells. Meanwhile, the amount of R2 suggested that the level of AMEE had a 95.08% influence on the rate of cell viability, and the other 4.92% influenced by factors other than the AMEE dose. These results indicated that the ethanol extract of soursop leaves has a cytotoxic effect and has the potential to inhibit T47D cell proliferation in vitro.

scholarly journals Efek Sitotoksik Ekstrak dan Fraksi Umbi Paku Atai Merah (Angiopteris ferox Copel) Terhadap Sel Kanker Payudara T47D

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Andi Nur Aisyah ◽  
Syamsu Nur ◽  
Endang Lukitaningsih ◽  
Rumiyati Rumiyati ◽  
Asril Burhan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Supportive Therapy ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Screening Tests

The use of natural products has been widely used as a resource of new bioactive chemical compounds. One of them is the Paku Atai Merah (Angiopteris ferox Copel) tuber which has long been used empirically by the Dayak tribe of East Kalimantan as an anti-cancer. The purpose of this study was to determine the anticancer cytotoxic activity of the extract and fractions of Paku Atai Merah tuber against T47D breast cancer cells in vitro. Extract of Paku Atai Merah tubers was obtained by maceration method using ethanol solvent until obtained the ethanolic extract then fractionated using various solvents to obtain n-hexane, ethyl acetate, and aqueous-ethanol fractions. The cytotoxic effect was carried out based on the MTT assay. Phytochemical screening tests showed positive results for the presence of flavonoid, phenolic, tannin, saponin and steroid compounds. The results of the cytotoxic activity study showed that the ethyl acetate fraction had moderate cytotoxic activity in T47D cancer cells with an IC50 value  of 84.8 µg/ml. Ethanol extract (513.06 µg/ml) and n-Hexane frsction (881.97 µg/ ml) were also included in the weak category. This study indicates that ethyl acetate fraction can be developed as a supportive therapy for breast cancer treatment.  

scholarly journals Sitotoksisitas in vitro ekstrak etanolik buah parijoto (Medinilla speciosa, reinw.ex bl.) terhadap sel kanker payudara T47D

2014 ◽  
Vol 2 (2) ◽  
pp. 53-58 ◽  
Author(s):  
Iin Tusanti ◽  
Andrew Johan ◽  
RA Kisdjamiatun
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Colorimetric Assay ◽  
Ethanolic Extract ◽  
T47d Cell ◽  
Ic50 Value ◽  
T47d Cell Line

Background: Several studies focused on phytochemical as agents of cancer prevention and co-chemotherapy. One of Indonesian plant which has edible fruit but it hasn’t been completely explored is Medinilla speciosa (Reinw.ex Bl.). Objective : The aim of this study is to examine the cytotoxic activity (IC50 value) of Medinilla speciosa (Reinw.ex Bl.) fruit ethanolic extract. Methods : Medinilla speciosa (Reinw.ex Bl.) fruit ethanolic extract was used in this study. The cytotoxic activity was investigated in vitro on human breast cancer T47D cell-line. The cells viability were assessed using MTT colorimetric assay. Breast cancer T47D cell lines was treated with fruit ethanolic extract (10, 25, 50, 100, 250, 500 and 1000 µg/ml) for 24 hour of incubation. This study also identified phytochemical compound of the fruit with thin layer chromatography (TLC). Results: The result showed that ethanolic extract of Medinilla speciosa (Reinw.ex Bl.) has moderate cytotoxicity on breast cancer T47D cell line with IC50 value of 614.50 µg/ml and yield the decrease of cell viability at higher concentration. Medinilla speciosa fruit can not be used as anticancer agent but chemoprevention agent. Phytochemical test showed that the fruit extract contain flavonoid and saponin compound. Conclusion: Ethanolic extract of Medinilla speciosa fruit exhibited moderate cytotocicity on breast cancer T47D cell lines with IC50 value was 614,50 µg/ml thus it can be used as chemopreventioan agent.

Design, Synthesis, In vitro Cytotoxic Activity Evaluation, and Study of Apoptosis Inducing Effect of New Styrylimidazo[1,2-a]Pyridines as Potent Anti-Breast Cancer Agents

2019 ◽  
Vol 19 (2) ◽  
pp. 265-275 ◽  
Author(s):  
Faeze Khalili ◽  
Sara Akrami ◽  
Malihe Safavi ◽  
Maryam Mohammadi-Khanaposhtani ◽  
Mina Saeedi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
High Yield ◽  
Breast Cancer Cell Lines ◽  
Pyridine Derivatives ◽  
One Pot ◽  
Standard Drug ◽  
Three Component Reaction ◽  
Anti Cancer

Background: This paper reports synthesis, cytotoxic activity, and apoptosis inducing effect of a novel series of styrylimidazo[1,2-a]pyridine derivatives. Objective: In this study, anti-cancer activity of novel styrylimidazo[1,2-a]pyridines was evaluated. Methods: Styrylimidazo[1,2-a]pyridine derivatives 4a-o were synthesized through a one-pot three-component reaction of 2-aminopyridines, cinnamaldehydes, and isocyanides in high yield. All synthesized compounds 4a-o were evaluated against breast cancer cell lines including MDA-MB-231, MCF-7, and T-47D using MTT assay. Apoptosis was evaluated by acridine orange/ethidium bromide staining, cell cycle analysis, and TUNEL assay as the mechanism of cell death. Results: Most of the synthesized compounds exhibited more potent cytotoxicity than standard drug, etoposide. Induction of apoptosis by the most cytotoxic compounds 4f, 4g, 4j, 4n, and 4m was confirmed through mentioned methods. Conclusion: In conclusion, these results confirmed the potency of styrylimidazo[1,2-a]pyridines for further drug discovery developments in the field of anti-cancer agents.

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