Application of matrix solid-phase dispersion–HPLC method to determine patulin in apple and apple juice concentrate

In this study, a green ionic-liquid based vortex-synchronized matrix solid-phase dispersion (VS-MSPD) combined with high performance liquid chromatography (HPLC) method was developed as a quantitative determination method for four anthraquinones in Cassiae Semen. Two conventional adsorbents, C18 and silica gel were investigated. The strategy included two steps: Extraction and determination. Wasted crab shells were used as an alternative adsorbent and ionic liquid was used as an alternative solvent in the first step. Factors affecting extraction efficiency were optimized: A sample/adsorbent ratio of 2:1, a grinding time of 3 min, a vortex time of 3 min, and ionic liquid ([Domim]HSO4, 250 mM) was used as eluent in the VS-MSPD procedure. As a result, the established method provided satisfactory linearity (R > 0.999), good accuracy and high reproducibility (RSD < 4.60%), and it exhibited the advantages of smaller sample amounts, shorter extraction time, less volume of elution solvent, and was much more environmental-friendly when compared with other conventional methods.

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Validated RP-HPLC Method for Simultaneous Determination of Tocopherols and Tocotrienols in Whole Grain Barley Using Matrix Solid-Phase Dispersion

Food Analytical Methods ◽

10.1007/s12161-014-9904-9 ◽

2014 ◽

Vol 8 (2) ◽

pp. 392-400 ◽

Cited By ~ 13

Author(s):

Emmanouil D. Tsochatzis ◽

Roxani Tzimou-Tsitouridou

Keyword(s):

Simultaneous Determination ◽

Solid Phase ◽

Hplc Method ◽

Whole Grain ◽

Matrix Solid Phase Dispersion ◽

Phase Dispersion ◽

Rp Hplc

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Determination of Multiclass Pesticide Residues in Apple Juice by Gas Chromatography-Mass Selective Detection after Extraction by Matrix Solid-Phase Dispersion

Journal of AOAC International ◽

10.1093/jaoac/87.4.972 ◽

2004 ◽

Vol 87 (4) ◽

pp. 972-985 ◽

Cited By ~ 4

Author(s):

Xiaozhong Hu ◽

Yu Jianxin ◽

Yan Zhigang ◽

Ni Lansun ◽

Lin Yanfei ◽

...

Keyword(s):

Gas Chromatography ◽

Apple Juice ◽

Solid Phase ◽

Single Injection ◽

Matrix Solid Phase Dispersion ◽

Phase Dispersion ◽

Determination Procedure ◽

Coefficients Of Variation ◽

Glass Column

Abstract A multiresidue method is described for the analysis of 106 multiclass pesticides (organochlorine, organophosphate, carbamate, pyrethroid, and triazine classes) in apple juice in a single injection. The determination procedure was based on matrix solid-phase dispersion of juice on diatomaceous earth in a glass column and subsequent extraction with a mixture of hexane–dichloromethane (1 + 1) at a flow rate of 5 mL/min. The analytes were determined by capillary gas chromatography with mass spectrometric detection and confirmed by their retention times and ion ratios. The coefficients of variation for analysis of samples fortified over the range of 0.01–0.2 mg/kg were 1.62 to 18.4%, and the recoveries for all analytes were between 70 and 110%.

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