Targeted quantification of B vitamins using ultra-performance liquid chromatography-selected reaction monitoring mass spectrometry in faba bean seeds

2021 ◽  
Vol 95 ◽  
pp. 103687
Author(s):  
Jeremy Marshall ◽  
Haixia Zhang ◽  
Hamid Khazaei ◽  
Kevin Mikituk ◽  
Albert Vandenberg
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Faba Bean ◽  
Ultra Performance Liquid Chromatography ◽  
B Vitamins ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring

scholarly journals Validated B Vitamin Quantification from Lentils by Selected Reaction Monitoring Mass Spectrometry

2020 ◽  
Author(s):  
Haixia Zhang ◽  
Devini De Silva ◽  
Dilanganie Dissanayaka ◽  
Thomas D. Warkentin ◽  
Albert Vandenberg
Keyword(s):  
Mass Spectrometry ◽  
Pantothenic Acid ◽  
Dry Weight ◽  
Ultra Performance Liquid Chromatography ◽  
Rapid Analysis ◽  
B Vitamins ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring ◽  
Rat Serum ◽  
B Vitamin

AbstractA validated method for B vitamin separation and quantification from lentil seeds using ultra-performance liquid chromatography-selected reaction monitoring mass spectrometry (UPLC-SRM MS) was reported. The use of three enzymes (acid phosphatase, β-glucosidase, and rat serum) with a 4 h incubation was sufficient to convert bound B vitamins into their free forms. Twenty B vitamers were selected and a 5-min UPLC-SRM MS method was optimized for rapid analysis. This method was applied to quantify B vitamin concentration during lentil seed germination over a 5-day period. Total B vitamins increased up to 1.5-fold on day 5 (from 39.2 µg/g to 60.6 µg/g of dry weight) comparing with dry seeds. Vitamin B5 (pantothenic acid) was the most abundant B vitamin in both dry seeds (34.2%) and in germinated seeds (17.7%-24.5% of total B vitamins); B8 (biotin) and B12 (cyanocobalamin) were not detected in lentil samples.

Assessment of semi-permanent hair dyes in wash water from beauty salons by liquid chromatography-tandem mass spectrometry-selected reaction monitoring (LC-MS/MS-SRM)

2020 ◽  
Vol 12 (44) ◽  
pp. 5415-5423
Author(s):  
Jefferson Honorio Franco ◽  
Bianca F. da Silva ◽  
Maria V. B. Zanoni
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Wash Water ◽  
Selected Reaction Monitoring ◽  
Tandem Mass ◽  
Reaction Monitoring ◽  
Hair Dyes ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Herein, we present an approach for the quantification of semi-permanent hair dyes in wash water samples released during washing of dyed hair employing a liquid chromatography-tandem mass spectrometry-selected reaction monitoring (LC-MS/MS-SRM).

Sign in / Sign up

Export Citation Format

Share Document