Amino acid determination by HPLC combined with multivariate approach for geographical classification of Malaysian Edible Bird’s Nest

ABSTRACT A protein isolated from ovine pituitary glands has been purified, and its homogeneity assessed by NH2- and COOH-terminal amino acid determination, ultracentrifugation studies, and polyacrylamide gel electrophoresis after carboxymethylation. Its chemical and immunochemical properties are closely similar to those of beef and pork neurophysins, less similar to those of human neurophysins. It contains no tryptophan (like other neurophysins) or histidine (like all except bovine neurophysin-I and human neurophysins). It has alanine at the NH2-terminus and valine at the COOH-terminus. Its amino acid composition is similar to, but not identical with those of porcine and bovine neurophysins.

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Determination of free amino acids in plants by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS)

Analytical Methods ◽

10.1039/c5ay01280e ◽

2015 ◽

Vol 7 (18) ◽

pp. 7574-7581 ◽

Cited By ~ 9

Author(s):

Magdalena M. Dziągwa-Becker ◽

Jose M. Marin Ramos ◽

Jakub K. Topolski ◽

Wiesław A. Oleszek

Keyword(s):

Mass Spectrometry ◽

Amino Acids ◽

Liquid Chromatography ◽

Amino Acid ◽

Tandem Mass Spectrometry ◽

Free Amino ◽

Tandem Mass ◽

Amino Acid Determination ◽

Acid Determination

Free amino acid determination in plants by LC-MS/MS.

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A new method for deproteinization of small samples of blood plasma for amino acid determination

Analytical Biochemistry ◽

10.1016/0003-2697(77)90153-1 ◽

1977 ◽

Vol 82 (1) ◽

pp. 236-239 ◽

Cited By ~ 49

Author(s):

L. Arola ◽

E. Herrera ◽

M. Alemany

Keyword(s):

Amino Acid ◽

Blood Plasma ◽

New Method ◽

Small Samples ◽

Amino Acid Determination ◽

Acid Determination

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Amino Acid Determination—Methods and Techniques

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/51.6.1345 ◽

1968 ◽

Vol 51 (6) ◽

pp. 1345-1345

Author(s):

J S Adkins

Keyword(s):

Amino Acid ◽

Methods And Techniques ◽

Amino Acid Determination ◽

Determination Methods ◽

Acid Determination

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ESSENTIAL AMINO ACIDS IN MICROORGANISMS

Canadian Journal of Microbiology ◽

10.1139/m57-080 ◽

1957 ◽

Vol 3 (5) ◽

pp. 721-728 ◽

Cited By ~ 13

Author(s):

F. Reusser ◽

J. F. T. Spencer ◽

H. R. Sallans

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Quantitative Method ◽

Essential Amino Acids ◽

Cellular Protein ◽

One Dimensional ◽

Low Concentrations ◽

Amino Acid Determination ◽

High Concentrations ◽

Acid Determination

The cells of 19 species of bacteria, actinomyces, and yeasts were analyzed for protein and essential amino acids. A rapid quantitative method for amino acid determination using one-dimensional paper chromatography was developed. The cellular protein from all species contained relatively high concentrations of lysine, somewhat lower concentrations of tryptophan and threonine, and very low concentrations of methionine. All of the 10 essential amino acids were found in each species tested, although individual differences in the relative and absolute amounts were observed.

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