Enhanced sulfate reduction with acidogenic sulfate-reducing bacteria

The vertical distribution of sulfate-reducing bacteria (SRB) in microaerophilic wastewater biofilms grown on fully submerged rotating disk reactors (RDR) was determined by the conventional culture-dependent MPN method and in situ hybridization of fluorescently-labelled 16S rRNA-targeted oligonucleotide probes for SRB in parallel. Chemical concentration profiles within the biofilm were also measured using microelectrodes for O2, S2-, NO3- and pH. In situ hybridization revealed that the SRB probe-stained cells were distributed throughout the biofilm even in the oxic surface zone in all states from single scattered cells to clustered cells. The higher fluorescence intensity and abundance of SRB probe-stained cells were found in the middle part of the biofilm. This result corresponded well with O2 and H2S concentration profiles measured by microelectrodes, showing sulfate reduction was restricted to a narrow anaerobic zone located about 500 μm below the biofilm surface. Results of the MPN and potential sulfate reducing activity (culture-dependent approaches) indicated a similar distribution of cultivable SRB in the biofilm. The majority of the general SRB probe-stained cells were hybridized with SRB 660 probe, suggesting that one important member of the SRB in the wastewater biofilm could be the genus Desulfobulbus. An addition of nitrate forced the sulfate reduction zone deeper in the biofilm and reduced the specific sulfate reduction rate as well. The sulfate reduction zone was consequently separated from O2 and NO3- respiration zones. Anaerobic H2S oxidation with NO3- was also induced by addition of nitrate to the medium.

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Interactions between filamentous sulfur bacteria, sulfate reducing bacteria and poly-P accumulating bacteria in anaerobic-oxic activated sludge from a municipal plant

Water Science & Technology ◽

10.2166/wst.1998.0725 ◽

1998 ◽

Vol 37 (4-5) ◽

pp. 599-603 ◽

Cited By ~ 4

Author(s):

Ryoko Yamamoto-Ikemoto ◽

Saburo Matsui ◽

Tomoaki Komori ◽

Edja. Kofi. Bosque-Hamilton

Keyword(s):

Activated Sludge ◽

Sulfate Reduction ◽

Reduction Rate ◽

Sulfate Reducing Bacteria ◽

Sulfate Reduction Rate ◽

Filamentous Bulking ◽

Sulfate Reducing ◽

Sulfur Bacteria ◽

Sulfate Reduction Rates ◽

Reducing Bacteria

The interactions between filamentous sulfur bacteria (FSB), sulfate reducing bacteria (SRB) and poly-P accumulating bacteria (PAB) in the activated sludge of a municipal plant operated under anaerobic-oxic conditions were examined in batch experiments using return sludge (RAS) and settled sewage. Phosphate release and sulfate reduction occurred simultaneously under anaerobic conditions. SRB were more sensitive to temperature changes than PAB. SRB played an important role in the decomposition of propionate to acetate. When the sulfate reduction rates were high, there was a tendency for the maximum release of phosphate also to be high. This was explained by the fact that PAB utilized the acetate produced by SRB. Sulfur oxidizing bacteria were sensitive to temperature change. When the sulfate reduction rate was high, the sulfide oxidizing rate was also high and filamentous bulking occurred. The results showed that sulfate reduction was a cause of filamentous bulking due to Type 021N that could utilize reduced sulfur.

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Biological sulfate reduction of a sulfate-rich industrial waste liquor using sulfate reducing bacteria

Mining Metallurgy & Exploration ◽

10.1007/bf03402463 ◽

2013 ◽

Vol 30 (4) ◽

pp. 205-211

Author(s):

K. A. Natarajan ◽

S. Usha Padukone

Keyword(s):

Sulfate Reduction ◽

Industrial Waste ◽

Sulfate Reducing Bacteria ◽

Waste Liquor ◽

Sulfate Reducing ◽

Biological Sulfate Reduction ◽

Reducing Bacteria

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Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People

Journal of Clinical Medicine ◽

10.3390/jcm9061920 ◽

2020 ◽

Vol 9 (6) ◽

pp. 1920 ◽

Cited By ~ 2

Author(s):

Ivan Kushkevych ◽

Jorge Castro Sangrador ◽

Dani Dordević ◽

Monika Rozehnalová ◽

Martin Černý ◽

...

Keyword(s):

Sulfate Reduction ◽

Biomass Accumulation ◽

Sulfate Reducing Bacteria ◽

Dissimilatory Sulfate Reduction ◽

Healthy Individuals ◽

Fecal Samples ◽

Sulfate Reducing ◽

Cell Extracts ◽

Production Of Hydrogen ◽

Reducing Bacteria

Background: Inflammatory bowel diseases (IBDs) are multifactorial illnesses of the intestine, to which microorganisms are contributing. Among the contributing microorganisms, sulfate-reducing bacteria (SRB) are suggested to be involved in the process of bowel inflammation due to the production of hydrogen sulfide (H2S) by dissimilatory sulfate reduction. The aims of our research were to physiologically examine SRB in fecal samples of patients with IBD and a control group, their identification, the study of the process of dissimilatory sulfate reduction (sulfate consumption and H2S production) and biomass accumulation. Determination of biogenic elements of the SRB and evaluation of obtained parameters by using statistical methods were also included in the research. The material for the research consisted of 14 fecal samples, which was obtained from patients and control subjects. Methods: Microscopic techniques, microbiological, biochemical, biophysical methods and statistical analysis were included. Results: Colonies of SRB were isolated from all the fecal samples, and subsequently, 35 strains were obtained. Vibrio-shaped cells stained Gram-negative were dominant in all purified studied strains. All strains had a high percentage of similarity by the 16S rRNA gene with deposited sequences in GenBank of Desulfovibrio vulgaris. Cluster analysis of sulfate reduction parameters allowed the grouping of SRB strains. Significant (p < 0.05) differences were not observed between healthy individuals and patients with IBD with regard to sulfate reduction parameters (sulfate consumption, H2S and biomass accumulation). Moreover, we found that manganese and iron contents in the cell extracts are higher among healthy individuals in comparison to unhealthy individuals that have an intestinal bowel disease, especially ulcerative colitis. Conclusions: The observations obtained from studying SRB emphasize differences in the intestinal microbial processes of healthy and unhealthy people.

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CHARACTERIZATION OF SULFATE REDUCING BACTERIA FROM BIOFILM SULFATE REDUCTION AND CU PRECIPITATION BIOREACTOR (BATCH CULTURE).

Journal of Academic Research and Sciences (JARES) ◽

10.30957/jares.v2i2.400 ◽

2018 ◽

Vol 2 (2) ◽

pp. 1

Author(s):

Tyas Nyonita Punjungsari

Keyword(s):

Community Structure ◽

Microbial Community ◽

Sulfate Reduction ◽

Batch Culture ◽

Microbial Community Structure ◽

Sulfate Reducing Bacteria ◽

Zeolite Surface ◽

Sulfate Reducing ◽

Reducing Bacteria

The biofilm is a microbial community structure formed on the zeolite surface in a sulfate reduction bioreactor and Cu deposition using a SRB consortium . The biofilm soluble microbial solvent is expected to have the capability in sulfate reduction and Cu deposition. Characterization of isolates is required for the optimization of pure culture . The aim of this study is to isolate and characterize the biofilm sulfate reducing bacteria in the sulfate reduction bioreactor and the precipitation of Cu in Batch Culture by a consortium of Sulfate Reducing Bacteria. The method used in this study cultivation was done by using postgate B medium, isolation was done by diluting biofilm on NaCl salt, bacteria grown on NB and postgate B media, characterization done by morphology and biochemistry. There were 3 isolates of B1 (Metylobacterium ), B3 ( Desulfucoccus ), and B2 ( Desulfobacter ). B3 ( Desulfococcus) has the best ability to reduce sulfate and Cu precipitation.Keywords : Sulfur Reducing Bacteria (SRB), Biofilm, Sulfate, Cu. Received: 26 August, 2017; Accepter: 10 September, 2017

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Symbiosis and competition among sulfate reduction, filamentous sulfur, denitrification, and poly-p accumulation bacteria in the anaerobic-oxic activated sludge of a municipal plant

Water Science & Technology ◽

10.2166/wst.1996.0543 ◽

1996 ◽

Vol 34 (5-6) ◽

pp. 119-128 ◽

Cited By ~ 10

Author(s):

Ryoko Yamamoto-Ikemoto ◽

Saburo Matsui ◽

Tomoaki Komori ◽

E. J. Bosque-Hamilton

Keyword(s):

Organic Acids ◽

Activated Sludge ◽

Sulfate Reduction ◽

Sulfide Oxidation ◽

Sulfate Reducing Bacteria ◽

Sulfur Cycle ◽

Sulfate Reducing ◽

P Accumulation ◽

Relationship Of ◽

Reducing Bacteria

Symbiosis and competition were examined among sulfate reducing bacteria (SRB), filamentous sulfur bacteria (FSB), denitrification bacteria (DNB) and poly-P accumulation bacteria (PAB) in the activated sludge of a municipal plant operated under anaerobic-oxic conditions. Batch experiments were carried out using settled sewage from the same plant as the substrate under several conditions. Under oxic conditions, both sulfate reduction and sulfide oxidation occurred simultaneously, making a symbiotic relationship of SRB and FSB for establishment of a sulfur cycle sustaining the energy requirements. Under anoxic conditions, denitrification was dominant because DNB outcompeted PAB and SRB for organic acids. Under anaerobic conditions, phosphate release and sulfate reduction occurred simultaneously. SRB produced for moles of acetate from four moles of propionate and/or unknown substances by reduction of three moles of sulfate. PAB competed with sulfate-reducing bacteria for organic acids such as propionate. However, PAB utilized acetate produced by SRB.

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Activity and Diversity of Sulfate-Reducing Bacteria in a Petroleum Hydrocarbon-Contaminated Aquifer

Applied and Environmental Microbiology ◽

10.1128/aem.68.4.1516-1523.2002 ◽

2002 ◽

Vol 68 (4) ◽

pp. 1516-1523 ◽

Cited By ~ 123

Author(s):

Jutta Kleikemper ◽

Martin H. Schroth ◽

William V. Sigler ◽

Martina Schmucki ◽

Stefano M. Bernasconi ◽

...

Keyword(s):

Carbon Source ◽

Sulfate Reduction ◽

Petroleum Hydrocarbon ◽

Carbon Sources ◽

Sulfate Reducing Bacteria ◽

Rate Coefficients ◽

Contaminated Aquifer ◽

Sulfate Reducing ◽

Pull Tests ◽

Reducing Bacteria

ABSTRACT Microbial sulfate reduction is an important metabolic activity in petroleum hydrocarbon (PHC)-contaminated aquifers. We quantified carbon source-enhanced microbial SO4 2− reduction in a PHC-contaminated aquifer by using single-well push-pull tests and related the consumption of sulfate and added carbon sources to the presence of certain genera of sulfate-reducing bacteria (SRB). We also used molecular methods to assess suspended SRB diversity. In four consecutive tests, we injected anoxic test solutions (1,000 liters) containing bromide as a conservative tracer, sulfate, and either propionate, butyrate, lactate, or acetate as reactants into an existing monitoring well. After an initial incubation period, 1,000 liters of test solution-groundwater mixture was extracted from the same well. Average total test duration was 71 h. We measured concentrations of bromide, sulfate, and carbon sources in native groundwater as well as in injection and extraction phase samples and characterized the SRB population by using fluorescence in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE). Enhanced sulfate reduction concomitant with carbon source degradation was observed in all tests. Computed first-order rate coefficients ranged from 0.19 to 0.32 day−1 for sulfate reduction and from 0.13 to 0.60 day−1 for carbon source degradation. Sulfur isotope fractionation in unconsumed sulfate indicated that sulfate reduction was microbially mediated. Enhancement of sulfate reduction due to carbon source additions in all tests and variability of rate coefficients suggested the presence of specific SRB genera and a high diversity of SRB. We confirmed this by using FISH and DGGE. A large fraction of suspended bacteria hybridized with SRB-targeting probes SRB385 plus SRB385-Db (11 to 24% of total cells). FISH results showed that the activity of these bacteria was enhanced by addition of sulfate and carbon sources during push-pull tests. However, DGGE profiles indicated that the bacterial community structure of the dominant species did not change during the tests. Thus, the combination of push-pull tests with molecular methods provided valuable insights into microbial processes, activities, and diversity in the sulfate-reducing zone of a PHC-contaminated aquifer.

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Competition for H2 between sulfate reducers, methanogens and homoacetogens in a gas-lift reactor

Water Science & Technology ◽

10.2166/wst.2002.0294 ◽

2002 ◽

Vol 45 (10) ◽

pp. 75-80 ◽

Cited By ~ 57

Author(s):

J. Weijma ◽

F. Gubbels ◽

L.W. Hulshoff Pol ◽

A.J.M. Stams ◽

P. Lens ◽

...

Keyword(s):

Kinetic Parameters ◽

Sulfate Reduction ◽

Growth Kinetic ◽

Settling Velocity ◽

Sulfate Reducing Bacteria ◽

Anaerobic Sludge ◽

Sulfate Reducers ◽

Sulfate Reducing ◽

Reducing Bacteria ◽

Gas Lift

Reported values for growth kinetic parameters show an order in competitivity of heterotrophic sulfate reducing bacteria>methanogens>homoacetogens for the substrate hydrogen. This order suggests that methanogens can succesfully compete with consortia of heterotrophic SRB and homoacetogens when H2/CO2 is present as sole substrate. However, we found in experiments using gas-lift reactors inoculated with anaerobic sludge and fed with H2/CO2 and sulfate, that heterotrophic sulfate reduction rapidly and completely outcompeted methanogenesis, whereas a low amount of acetate was formed. Thus, in disagreement with the above competitivity order, hydrogen is more readily consumed by homoacetogenesis than by methanogenesis, indicating that the competition is not kinetically determined. The superior settling velocity of sulfidogenic-acetogenic sludge compared to that of methanogenic sludge suggests that the former sludge is better retained, which can explain the predominance of sulfate reduction/homoacetogenesis over methanogenesis.

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