Green molybdenum nanoparticles-mediated bio-stimulation of Bacillus sp. strain ZH16 improved the wheat growth by managing in planta nutrients supply, ionic homeostasis and arsenic accumulation

The Na+/K+ ATPase is a key regulator of the hepatocytes ionic homeostasis, which when altered may lead to many liver disorders. We demonstrated recently, a significant stimulation of the Na+/K+ ATPase in HepG2 cells treated with the S1P analogue FTY 720P, that was mediated through PGE2. The mechanism by which the prostaglandin exerts its effect was not investigated, and is the focus of this work. The type of receptors involved was determined using pharmacological inhibitors, while western blot analysis, fluorescence imaging of GFP-tagged Na+/K+ ATPase, and time-lapse imaging on live cells were used to detect changes in membrane abundance of the Na+/K+ ATPase. The activity of the ATPase was assayed by measuring the amount of inorganic phosphate liberated in the presence and absence of ouabain. The enhanced activity of the ATPase was not observed when EP4 receptors were blocked but still appeared in presence inhibitors of EP1, EP2 and EP3 receptors. The involvement of EP4 was confirmed by the stimulation observed with EP4 agonist. The stimulatory effect of PGE2 did not appear in presence of Rp-cAMP, an inhibitor of PKA, and was imitated by db-cAMP, a PKA activator. Chelating intracellular calcium with BAPTA-AM abrogated the effect of db-cAMP as well as that of PGE2, but PGE2 treatment in a calcium-free PBS medium did not, suggesting an involvement of intracellular calcium, that was confirmed by the results obtained with 2-APB treatment. Live cell imaging showed movement of GFP–Na+/K+ ATPase-positive vesicles to the membrane and increased abundance of the ATPase at the membrane after PGE2 treatment. It was concluded that PGE2 acts via EP4, PKA, and intracellular calcium.

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EKSPLORASI DAN UJI VIRULENSI BAKTERI Bacillus sp. ENDOFIT JAGUNG TERHADAP PENYAKIT BUSUK PELEPAH JAGUNG

Jurnal Ilmu-Ilmu Pertanian Indonesia ◽

10.31186/jipi.22.2.70-78 ◽

2020 ◽

Vol 22 (2) ◽

pp. 70-78

Author(s):

Arum Saputri ◽

Loekas Soesanto ◽

Abu Umayah ◽

Agus Sarjito

Keyword(s):

Plant Height ◽

Endophytic Bacteria ◽

Block Design ◽

Pathogenic Fungi ◽

In Vitro Test ◽

Bacillus Sp ◽

In Planta ◽

Fresh Weight ◽

Randomized Block Design

[EXPLORATION AND VIRULENT TEST OF MAIZE ENDOPHYTE Bacillus sp. AGAINST MAIZE SHEATH BLIGHT]. Capability of endophytic bacterial, effectivity, and its effect on R. solani. and on maize seedlings growth were investigated from April 2018 to January 2019. Exploration of endophytes bacteria in maize was taken from Banyumas Regency (Sumbang, Kembaran, Baturraden) and Purbalingga Regency (Padamara, Bojongsari, Pratin). Taking plant samples using Purposive Random Sampling and Diagonal Sampling methods. Completely randomized design was used in in vitro test with 16 treatments repeated twice. Completely randomized block design was used in in planta experiment with 5 treatments repeated 5 times. The treatment consisted of control, fungiside (mankozeb), and 2 isolates of endophytes bacteria performing the best in vitro result. Variables observed included characteristics of endophytic bacteria and pathogenic fungi, inhibition diameter, incubation period, disease intensity, incidence of disease, AUDPC, plant height, leaf number, root length, plant fresh weight, canopy fresh weight, and root fresh weight. Resultshowed that the exploration obtained 15 endophytic Bacillus sp. isolates. The PD A.4 and BK A.1 isolates werw able to inhibit the growth of pathogenic fungi in-vitro by 56.93 and 51.5%, respectively. The soaking treatment using BK A1 was able to reduce disease intensity by 59.377%, and AUDPC value 34.19%. Endophytic bacteria influence plant height, plant fresh weight, canopy fresh weight, and fresh weight of roots respectively as 89.17 cm, 126.06 g, 106.67 g and 19.4 g.

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Karakterisasi Isolat Bakteri Endofit Nilam (Pogostemon cablin B.) sebagai Kandidat Biostimulan Pertumbuhan Tanaman

Buletin Plasma Nutfah ◽

10.21082/blpn.v27n1.2021.p21-28 ◽

2021 ◽

Vol 27 (1) ◽

pp. 21

Author(s):

Rafika Yuniawati ◽

Alina Akhdiya

Keyword(s):

Bacillus Sp ◽

In Planta ◽

Pogostemon Cablin

<p>Simbiosis bakteri endofit pada tanaman nilam telah diketahui berpotensi untuk menstimulasi pertumbuhan tanaman. Penelitian ini bertujuan untuk menyeleksi dan mengarakterisasi kemampuan bakteri endofit nilam (Pogostemon cablin B.) dalam menstimulasi pertumbuhan tanaman nilam. Bakteri endofit diisolasi dari beberapa sumber tanaman nilam, yaitu koleksi klon B6 (koleksi BB Biogen), varietas Sidikalang dan Patchoulina. Isolat bakteri endofit yang diisolasi dan diseleksi berdasarkan beberapa pengujian selanjutnya dikarakterisasi secara fisiologi, biokimia, dan genetik. Karakterisasi fisiologi dan biokimia meliputi uji aktivitas penghambatan bakteri fitopatogen, uji potensi fiksasi N dan pelarut fosfat, serta uji penghasil IAA-like compound, sedangkan karakterisasi genetik dilakukan berdasarkan analisis sekuensing 16srRNA. Hasil penelitian yang diperoleh menunjukkan empat belas isolat terdeteksi menghasilkan IAA like compounds. Empat dari 29 isolat tidak berpotensi patogenik, yaitu isolat B63.8,<br />B63.10, NSD 20, dan P 35. Isolat-isolat ini mampu memfiksasi nitrogen dan melarutkan fosfat. NSD 20 merupakan isolat yang paling potensial untuk dikembangkan sebagai kandidat biostimulan. Berdasarkan hasil karakterisasi genetik, isolat NSD 20 ini menunjukkan kemiripan tertinggi dengan Bacillus sp. Y14 (93,95%). Uji lanjut in planta di rumah kaca dan lapang diperlukan<br />untuk mengetahui kemampuan stimulasi pertumbuhan tanaman nilam oleh Bacillus sp. NSD 20.</p>

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Regulation of Positive-Strand Accumulation by Capsid Protein During Brome mosaic virus Infection In Planta

Phytopathology ◽

10.1094/phyto-07-19-0236-fi ◽

2020 ◽

Vol 110 (1) ◽

pp. 228-236 ◽

Cited By ~ 4

Author(s):

Mélissanne de Wispelaere ◽

Venkatesh Sivanandam ◽

A. L. N. Rao

Keyword(s):

Mosaic Virus ◽

Brome Mosaic Virus ◽

Open Reading Frames ◽

Detectable Effect ◽

In Planta ◽

Coding Region ◽

Protein Coding ◽

Severe Reduction ◽

Rna Stabilization ◽

Stimulation Of

A hallmark feature of (+)-strand RNA viruses of eukaryotic cells is that progeny (+)-strands are accumulated 100-fold over (−)-strands. Previous experimental evidence suggests that, in Brome mosaic virus (BMV), a plant-infecting member of the alphavirus-like superfamily, the addition of RNA3 and, specifically, translation of the wild-type (WT) coat protein (CP) gene contributes to increased accumulation of (+)-strands. It is unclear whether this stimulation of (+)-strand accumulation by CP is due to direct regulation of viral RNA replication or RNA stabilization via encapsidation. Analysis of BMV progeny RNA in Nicotiana benthamiana plants revealed that expression of RNA3 variants that did not express WT CP led to a severe defect in BMV (+)-strand accumulation. The (+)-strand accumulation could be rescued when CP was complemented in trans. To verify whether stimulation of (+)-strand accumulation is coupled with encapsidation, two independent mutations were engineered into CP open reading frames. An N-terminal deletion that prevented CP binding to the viral RNAs resulted in a severe reduction of BMV (+)-strand accumulation but stimulated (−)-strand accumulation over the WT. On the other hand, a C-terminal mutation affecting CP dimerization caused a significant decrease in (+)-strand accumulation but had no detectable effect on (−)-strand accumulation. Nucleotide sequences in the movement protein-coding region were also found to contribute to (+)-strand accumulation, in part by providing packaging signals for efficient RNA3 encapsidation. Overall, these results show that RNA encapsidation is a significant determinant of BMV RNA intracellular accumulation.

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SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.213167-178 ◽

2013 ◽

Vol 13 (2) ◽

pp. 167-178

Author(s):

Zurai Resti ◽

Trimurti Habazar ◽

Deddi Prima Putra ◽

Nasrun Nasrun

Keyword(s):

Bacillus Cereus ◽

Endophytic Bacteria ◽

Disease Incidence ◽

Leaf Blight ◽

Bacterial Leaf Blight ◽

Bacillus Sp ◽

In Planta ◽

16S Rrna Sequence ◽

Randomized Design ◽

Blight Disease

Screening and identification of endophytic bacteria to control bacterial leaf blight disease on Shallot. The experiment was conducted in Laboratory and Green House, from January to June 2012. Laboratory experiment consisted of three steps: (1) isolation of endophytic bacteria from healthy onion roots, (2)In planta /screening of endophytic isolates capable of reducing bacterial leaf blight disease, and (3) molecular identification of potential endophytic isolates. Treatments of in planta test were arranged in Completely Randomized Design. Collected isolates were tested for their capability in controlling bacterial leaf blight disease on shallot. The variables observed were disease incidence, disease severity, and shallot yield. The results showed that out of 82 isolates successfully isolated, 56 isolates (68.29%) were Gram positive, and 26 isolate (31.71%) were Gram negative. All isolates were HR negative and pathogenicity negative. Six endophytic isolates showed better performance in inducing resistance and increasing onion yield. Based on 16S rRNA sequence the six isolates were Bacillus cereus strain P14 , Bacillus cereus strain Se07, Bacillus sp H1, Bacillus sp SJ1 and Serratia marcescens strain PPM4.

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CHARACTERIZATION OF ENDOPHYTIC BACILLUS ISOLATED FROM SHALLOT ROOT AS BIOCONTROL OF BACTERIAL LEAF BLIGHT DISEASE

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.11831-38 ◽

2018 ◽

Vol 18 (1) ◽

pp. 31

Author(s):

Zurai Resti ◽

Trimurti Habazar ◽

Deddi Prima Putra ◽

Nasrun .

Keyword(s):

Salicylic Acid ◽

Endophytic Bacteria ◽

Root Colonization ◽

Linear Regression Analysis ◽

Leaf Blight ◽

Bacterial Leaf Blight ◽

Bacillus Sp ◽

In Planta ◽

Blight Disease

Characterization of Endophytic Bacillus Isolated from Shallot Root as Biocontrol of Bacterial Leaf Blight Disease. Endophytic Bacillus isolated from the roots of healthy shallots has potential as biological control agent of bacterial leaf blight (BLB) disease. Based on the in planta screening, four endophytic bacteria that capable of controlling BLB diseases from the group of Bacillus were obtained, such as Bacillus cereus P14, Bacillus cereus Se07, Bacillus sp. HI, and Bacillus sp. SJI. The aims of this study were to investigate the characteristics of endophytic Bacillus that capable of controlling BLB disease and to determine the characteristic that most responsible in the disease control. This study method was descriptive. The characteristics observed were production of antibiotics by paper disc method, production of salicylic acid by capillary electrophoresis, and root colonization by in planta method. Linear regression analysis was used to determine the characteristic most responsible in the disease control. The results showed that four endophytic Bacillus were capable of producing salicylic acid and colonizing the root, and three of them were also capable of producing antibiotic. Salicylic acid production varied ranging from 13.96 to 14.72 ppm mL-1. Three endophytic Bacillus were capable of producing antibiotic with inhibition zone of 16.25 to 20.25 mm. Endophytic Bacillus was able to colonize the shallot roots with a population of bacteria ranged from 3.20 × 105-6, 20 × 105 CFU g-1 root. Based on the correlation coefficient of linear regression analysis, the root colonization of endophytic bacteria played the role in decreasing BLB disease.

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