scholarly journals EKSPLORASI DAN UJI VIRULENSI BAKTERI Bacillus sp. ENDOFIT JAGUNG TERHADAP PENYAKIT BUSUK PELEPAH JAGUNG

2020 ◽  
Vol 22 (2) ◽  
pp. 70-78
Author(s):  
Arum Saputri ◽  
Loekas Soesanto ◽  
Abu Umayah ◽  
Agus Sarjito

[EXPLORATION AND VIRULENT TEST OF MAIZE ENDOPHYTE Bacillus sp. AGAINST MAIZE SHEATH BLIGHT]. Capability of endophytic bacterial, effectivity, and its effect on R. solani. and on maize seedlings growth were investigated from April 2018 to January 2019. Exploration of endophytes bacteria in maize was taken from Banyumas Regency (Sumbang, Kembaran, Baturraden) and Purbalingga Regency (Padamara, Bojongsari, Pratin). Taking plant samples using Purposive Random Sampling and Diagonal Sampling methods. Completely randomized design was used in in vitro test with 16 treatments repeated twice. Completely randomized block design was used in in planta experiment with 5 treatments repeated 5 times. The treatment consisted of control, fungiside (mankozeb), and 2 isolates of endophytes bacteria performing the best in vitro result. Variables observed included characteristics of endophytic bacteria and pathogenic fungi, inhibition diameter, incubation period, disease intensity, incidence of disease, AUDPC, plant height, leaf number, root length, plant fresh weight, canopy fresh weight, and root fresh weight. Resultshowed that the exploration obtained 15 endophytic Bacillus sp. isolates. The PD A.4 and BK A.1 isolates werw able to inhibit the growth of pathogenic fungi in-vitro by 56.93 and 51.5%, respectively. The soaking treatment using BK A1 was able to reduce disease intensity by 59.377%, and AUDPC value 34.19%. Endophytic bacteria influence plant height, plant fresh weight, canopy fresh weight, and fresh weight of roots respectively as 89.17 cm, 126.06 g, 106.67 g and 19.4 g.

2021 ◽  
Vol 21 (2) ◽  
pp. 82-90
Author(s):  
Loekas Soesanto ◽  
Lintang Yunita Sari ◽  
Endang Mugiastuti ◽  
Abdul Manan

Cross application of entomopathogenic fungi raw secondary metabolites for controlling fusarium wilt of chili seedlings. Theresearch aimed to determine the effect of entomopathogenic fungi raw secondary metabolites on fusarium wilt on chili plants and on growth of chili. In vitro test used a Completely Randomized Design with 5 treatments and 5 replicate and in planta using a Randomized Block Design with 5 treatments and 5 replicatie including control, secondary metabolites of Beauveria bassiana B10, B. bassiana B16, Metarhizium anisopliae M16, dan Lecanicillium lecanii L16. Variables observed included inhibition ability, incubation period, desease intensity, plant height, root length, and phenolic compounds (tannins, saponin, and hydroquinone) content qualitatively. The results showed that secondary metabolites of B. bassiana B10, B. bassiana B16, M. anisopliae M16, and L. lecanii L16 were able to inhibit growth of Fusarium oxysporum f.sp. capsici by 50.62; 50,64; 48,62; 56.62%, respectively, extend incubation periods of 71.05; 73,38; 64.89; and 68.57%, respectively, suppress disease intensity by 99.99; 99.99; 99.99; and 99.99%, respectively, can increase plant height by 15.22; 18.8; 21.14; 21.69%, respectively, increasing the root length by 22.61; 25,71; 26,34; 33.50%, respectively, and can increase the content of tannins, saponins and hydroquinone compounds qualitatively compared to controls. The secondary metabolites of enthomopathogenic fungi could be used as organic control for soilborne pathogenic fungi.


2021 ◽  
Vol 21 (2) ◽  
pp. 82-90
Author(s):  
Loekas Soesanto ◽  
Lintang Yunita Sari ◽  
Endang Mugiastuti ◽  
Abdul Manan

The research aimed to determine the effect of entomopathogenic fungi raw secondary metabolites on fusarium wilt on chili plants and on growth of chili. In vitro test used a Completely Randomized Design with 5 treatments and 5 replicate and in planta using a Randomized Block Design with 5 treatments and 5 replicatie including control, secondary metabolites of Beauveria bassiana B10, B. bassiana B16, Metarhizium anisopliae M16, dan Lecanicillium lecanii L16. Variables observed included inhibition ability, incubation period, desease intensity, plant height, root length, and phenolic compounds (tannins, saponin, and hydroquinone) content qualitatively. The results showed that secondary metabolites of B. bassiana B10, B. bassiana B16, M. anisopliae M16, and L. lecanii L16 were able to inhibit growth of Fusarium oxysporum f.sp. capsici by 50.62; 50,64; 48,62; 56.62%, respectively, extend incubation periods of 71.05; 73,38; 64.89; and 68.57%, respectively, suppress disease intensity by 99.99; 99.99; 99.99; and 99.99%, respectively, can increase plant height by 15.22; 18.8; 21.14; 21.69%, respectively, increasing the root length by 22.61; 25,71; 26,34; 33.50%, respectively, and can increase the content of tannins, saponins and hydroquinone compounds qualitatively compared to controls. The secondary metabolites of enthomopathogenic fungi could be used as organic control for soilborne pathogenic fungi.


2020 ◽  
Vol 16 (1) ◽  
pp. 44
Author(s):  
Hanisa Desy Ariani ◽  
Noor Aidawati ◽  
Dewi Arika Adriani

One of the causes of the declining productivity of rice is sheath blight disease caused by the mold Rhizoctonia solani Kuhn. Control of sheath blight disease that is often done by the farmers is by using chemical pesticides (fungicides), which caused environmental problems. One way to reduce the use of pesticides is to biological control by using antagonist bacteria. This study aimed at in vitro test of rhizobacteria in preventing the development of sheath blight disease in rice plants. This research was conducted in the Phytopathology laboratory of Plant Protection Department of Faculty Agriculture, University of Lambung Mangkurat Banjarbaru from March to May 2018. The experiment used a randomized block design with three groups consisting of eight types of rhizobacteria isolates: (r1) Pseudomonas aeruginosa (Barito Kuala), (r2) Bacillus megaterium (Hulu Sungai Tengah), (r3) Azotobacter sp. (Barito Kuala), (r4) Pseudomonas sp. (Hulu Sungai Selatan), (r5) Flavobacterium sp. (Tanah Laut), (r6) Bacillus bodius (Barito Kuala), (r7) Pseudomonas aeruginosa (Hulu Sungai Selatan), (r8) Necercia sp. (Tanah Laut). The results showed that all rhizobacteria have the ability to inhibit the development of R. solani with different percentages of inhibitions. Pseudomonas aeruginosa (Barito Kuala) was the most effective rhizobacteria in inhibiting the development of R. solani.


Jurnal Agro ◽  
10.15575/810 ◽  
2016 ◽  
Vol 3 (1) ◽  
pp. 36-42
Author(s):  
Ida Nur Istina

Bawang merah merupakan salah satu komoditas hortikultura strategis yang penyebarannya hampir di seluruh wilayah Indonesia. Permasalahan pengembangan komoditas ini adalah masih rendahnya produktivitas sebagai akibat adaptasi dan kecukupan asupan hara tanaman. Penelitian yang bertujuan untuk mendapatkan jenis pupuk NPK yang efektif dan efisien telah dilakukan di desa Langensari Kecamatan Lembang Kabupaten Bandung Barat dari Maret sampai Mei 2014 menggunakan Rancangan Acak kelompok (RAK) dengan 6 perlakuan dan 3 kali ulangan. Pupuk NPK yang digunakan adalah A= NPK 18+9+10+Te, B=NPK 15+15+sulfat 10, C=NPK 12+11+18z+(S) z +3 Mg+3,8S+Te, D= NPK 15+9+20(S)+2 MgO+3,8 S+Te, E= NPK 25+7+7 dan F=kontrol. Parameter yang diamati meliputi: tinggi tanaman, jumlah umbi, jumlah daun, panjang umbi (cm), diameter umbi (cm), bobot basah (g) dan bobot kering brangkasan (g). Hasil penelitian menunjukkan bahwa NPK 12+11+18z+(S)z+3 Mg+3,8S+Te menghasilkan bobot umbi terbaik. The shallot is one of the strategic and valuable horticultural commodities which is spreaded almost all over Indonesia area. Commodity development constrain by the low productivity as a result of adaptation and inadequate intake of plant nutrients. The research purposed  to get the kind of NPK fertilizers that was efective and efficient on shallot production had been done in the Langensari village Langensari Lembang district, West Bandung regency from March till May 2014, using Randomized Block Design (RBD) with 6 treatments and 3 repplications. NPK fertilizer used were: A = NPK 18+9+10+Te, B = NPK 15+15+sulfate 10, C = NPK 12+11+18z+(S) z + 3 Mg+3,8S+Te, D = NPK 15+9+20(S)+2MgO+3,8 S+Te, E = NPK 25+7+7 and F = control. The observed parameters were plant height (cm), number of tubers, leaf number, tuber length, tuber diameter, fresh weight and dry weight tuber. The results showed that NPK 12+11+18z+ (S)z+3 Mg+3,8S+Te gave the best growth and production.


Agrika ◽  
2020 ◽  
Vol 14 (2) ◽  
pp. 201
Author(s):  
Palupi Puspitorini ◽  
Eko Wahyu Budiman

ABSTRAKPenelitian ini mempunyai tujuan untuk  mengetahui  durasi perendaman bibit   dengan auxin alami dan dosis pupuk NPK yang tepat  pada pertumbuhan dan hasil tanaman bawang merah (Allium ascalonicum L). Dalam budidaya bawang merah sering dijumpai pertumbuhan benih yang lambat disebabkan karena faktor dalam bibit (endogenous factor) yang merugikan karena akan berdampak pada serangan penyakit busuk umbi. Maka mempercepat munculnya tunas dengan perlakuan pemberian auxin dan pemberian pupuk NPK ini diharapkan akan diperoleh bibit yang segera tumbuh supaya dapat berproduksi dalam keadaan sehat. Pada penelitian ini akan dikaji bagaimana mempercepat pertumbuhan bibit bawang merah dengan perendaman cepat dalam auxin alami yang diinteraksikan dengan pemberian pupuk NPK. Penelitian disusun dalam  RAK  faktorial dengan 2 faktor sebanyak 3 x ulangan. Faktor 1 adalah perlakuan lama perendaman bibit bawang merah (D), yang terdiri dari D0 = tanpa perendaman , D1= perendaman 0,5 jam, D2 = perendaman 1 jam, D3= perendaman 1,5 jam, D4 = perendaman 2 jam. Sedangkan faktor kedua  adalah pemberian pupuk NPK, P1= 100 kg/ha, P2= 200 kg/ha, P3= 300 kg/ha.  Variabel yang diamati adalah tinggi tanaman, jumlah batang/rumpun, bobot segar bawang merah/rumpun, jumlah umbi bawang merah/rumpun. Data dianalisis dengan menggunakan Anova dan uji DMRT 5%.  Hasil Penelitian didapatkan  bahwa ada pengaruh interaksi nyata antara perlakuan D (lama perendaman) dan P (dosis pupuk NPK) pada variabel tinggi tanaman, jumlah batang/rumpun, bobot segar bawang merah/rumpun dan jumlah umbi/rumpun.  Perlakuan terbaik adalah D4P3 yaitu perendaman bibit bawang merah selama 2 jam dengan pemupukan NPK anorganik 300 kg/ha.  ABSTRACTThe aims of the study was to  determine  the duration of bulb dipping with  natural auxin on the growth and yield of shallot (Allium ascalonicum L). In shallot cultivation, it is found that the slow growth of bulb is caused by the factors in the bulbs (endogenous factors) because it will impact on bulb rot disease. Then accelerating the emergence of shoots with the treatment of auxin and NPK fertilizer, it is hoped that the bulbs will grow well so that they will be harvested in a healthy condition. In this research, we will study how to accelerate the growth of shallot bulbs with rapid immersion in natural auxin which is interacted with NPK fertilizer. The research was  arranged  in a factorial randomized block design with 2 factors and repeated 3 times, where the first factor was the treatment was the length of dipping the shallot seeds. (D). consisting of D0 = without dipping, D1 = dipping 0.5 hours, D2 = dipping 1 hour, D3 = dipping 1.5 hours, D4 = dipping 2 hours. While the second factor is the provision of  . NPK . fertilizer, P1 = 100 kg / ha, P2 = 200 kg / ha, P3 = 300 kg.ha. The variables observed were plant   height, number of clumps / plant, fresh weight of shallots / plant, number of shallot bulbs / plant. Observation data  were  analyzed by ANOVA and 5% DMRT test. The results showed that there was a significant   interaction effect between D and P treatment on the variable plant height, number of clumps/plant, fresh weight of shallots / plants and number of tubers / plants. The best treatment is D4P3, which is soaking shallot seeds for 2 hours with 300 kg / ha of inorganic NPK fertilization. 


2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Dida Alimin ◽  
Taufan Purwokusumaning Daru ◽  
Penny Pujowati

ABSTRAKPenelitian ini bertujuan untuk mengetahui 1) dosis pupuk kompos yang dapatmenghasilkan rumput meksiko tertinggi pada tanah top soil dan overburden, 2) produksi rumput yang ditanam pada tanah top soil dan overburden. Penelitian dilaksanakan mulai September 2016 sampai dengan Januari 2017. Penelitian dilakukan di Jalan Mugirejo RT. 14 Kelurahan Mugirejo, Kecamatan Sei Pinang Samarinda. Penelitian menggunakan Rancangan Acak Kelompok, dengan dua media tanam, dimana masing-masing media tanam diberi dosis pupuk kompos yang berbeda dan diulangan sebanyak 5 kali. Parameter yang diamati adalah tinggi tanaman, jumlah anakan, berat segar, dan berat kering tanaman. Hasil penelitian menunjukkan perlakuan pemupukan kompos dengan dosis 300 g polybag-1 menghasilkan hijaun rumput meksiko lebih tinggi dibandingkan dengan hasil hijauan rumput meksiko pada perlakuan dosis yang lainnya pada media tanam top soil dan overburden. Produksi rumput meksiko yang ditanam pada media tanam top soil menghasilkan rata-rata hijauan sebesar 1.390 g polybag-1 dan pada media tanam tanah overburden menghasilkan hijauan 1.320 g polybag-1. Perlakuan pemberian dosis pupuk kompos yang berbeda menunnjukkan adanya perbedaan yang nyata pada parameter tinggi tanaman, berat segar, dan berat kering.Kata kunci : Rumput meksiko, top soil, tanah overburden, pupuk kompos ABSTRACTThe purpose of this research is to know 1) the dosage of compost fertilizer that can produce the highest grass of mexico on ta top soil and overburden media, 2) grass production planted on top soil and overburden soil. The study was conducted from September 2016 to January 2017. The study was conducted at Jalan Mugirejo RT. 14 Mugirejo Sub-district, Sei Pinang District of Samarinda. The experiment using Randomized Block Design. These treatments use two media planting with each planting medium given the dose of composted fertilizer. All Treatments were replicated by 5 times. The results showed that compost fertilizer treatment with dosage 300 g polybag-1 gave highest fresh weight compared with other treatments (0, 100, and 200 g polybag-1). The average yield of fresh weight of mexican grass planted on top soil was 1.390 gpolybag-1 and planted on overburden soil was 1.320 g polybag-1. Treatment of different doses of compost fertilizer showed a significant effect on the parameters of plant height, fresh weight, and dry weight.Keywords: Mexican grass, top soil, over burden soil, compost fertilizer


1970 ◽  
Vol 5 (2) ◽  
pp. 177-182
Author(s):  
Jepriwira K. Ginting ◽  
Jonis Ginting ◽  
Nini Rahmawati

The research aims to determine the growth and production response of two varieties of lettuce (Lactuca sativa L.) to the provision of various sources of nitrogen. This research was conducted in Lambar village, Tigapanah sub-district, Karo regency, Sumatera Utara Province with altitude of ± 1200 meters above sea level, which started on July to September 2017. This research used a factorial randomized block design with 2 factors namely varieties of lettuce (crop and lettuce) and sources of nitrogen (urea fertilizer, chicken manure and Liquid organic fertilizer of rabbit urine). The parameters observed were plant height, greenish leaves, crop circle, fresh weight of plant per sample and fresh weight of plant per plot. The results showed that both varieties were significantly different in the parameters of plant height, greenish leaves, the number of leaves, and the fresh weight of the plant per sample. Application of nitrogen source showed differences in plant height at 10 weeks after move planting and fresh weight of plant per sample.


2019 ◽  
Vol 19 (3) ◽  
pp. 206
Author(s):  
Prasasti Dame Aritonang ◽  
Ardian Ardian ◽  
Kukuh Setiawan

This study aimed to evaluate the effect of paclobutrazol and KOH application through leaf togrowth and production of cassava.  This study was conducted from March 2017 to October 2018 in an Integrated Field Universityof Lampung. The treatmens was arranged factorially (4 x 4) in a complete randomized block design with 4 replications be avowed as group.  The main factor were paclobutrazol consist of P1 = 0 ppm (control, no treatment), P2 = 400 ppm, P3 = 500 ppm and P4 = 600 ppm.  The second factor were KOH consist of K1 = 0% (control, no treatment), K2 =0,5%, K3 = 1% and K4 = 1,5 given a week after paclobutrazol’s application.  This study used cassava cutting varieties of kasesart.  Observation variables were plant height, number of books, number of fresh leaves, fresh weight of leaves, dry weight of leaves, fresh weight of stem, dry weight of stem, fresh weight of tubers, dry weight of tubers.  Data of each treatments were calculated its mean value and tested homogeneity.  Homogeneous data were analyzed variance and followed by the smallest real difference testat level 5%.The study’s result showed that paclobutrazol application had inhibited effect the vegetative growth of plant height, number of nodes, fresh and dry weight of stem cassava plants.  Application of KOH had a significant effect only on number of fresh leave at 13 week after planted.  The interaction of paclobutrazol and KOH treatment had a significant effect on leaf dry weight.   


Author(s):  
Ramal Yusuf ◽  
Abdul Syakur ◽  
Budiatno Budiatno ◽  
Hidayati Mas'ud

Shallot plant is often used as a flavor enhancer and appetite enhancer food. Research on shallotcrop has been done. Application of seaweedsgive a different effect on increasing the productivity and growth of shallots. This study aims to determine the effect of applying different types of seaweed on growth and yield of shallot variety Lembah Palu. This research was conducted at the Green House of Faculty of Agriculture, University of Tadulako from March to May 2016. The study conducted using a randomized block design (RBD).  The treatments: B0 = control, B1 = NPK 0.2 g / pot, B2 = (Cauelerpa sp) 100 ml/pot, B3 = (Sargassum sp) 100 ml/pot, B4 = (Eucheuma cottonii) 100 ml /pot, B5 = Sea lettuce (Ulva sp) 100 ml/pot. there are six treatments, each treatment was replicated four times, so there are 24 experimental units. The results indicatedthat the application of various types of seaweed significant effect on plant height, fresh weight of the plants, the fresh weight of tuber and diameter of the bulb.


Author(s):  
M.I. Adebola, A.E. Esson

In this study, two accessions of Bambara groundnut were exposed to fast neutrons Americium-Beryllium (AmBe) source with flux of 1.5 ×104 ncm-2 s-1for different hours, and planted in a completely randomized block design (RCBD) with three replications to raise M1 generation. The data recorded were percentage germination, plant height, number of leaves, leaf length, leaf width, days to 50% flowering, number of pods per plant, fresh weight of plants, dry weight of plants, dry weight of pods, weight of seeds and100 seeds weight. There was variation in plant height, number of leaves, leaf length, leaf width, Days to 50% flowering, fresh weight of plants, dry weight of plants, dry weight of pods, weight of seeds and100 seeds weight for Black accession. In the Cream accession, days to 50% flowering, number of pods per plant, fresh weight of plants, dry weight of plants, dry weight of pods, weight of seeds and100 seeds weight were significantly different. Treatment at 16 hours performed best for plant height, leaf length, number of leaves, weight of seed per plant and 100 seed weight in Black accession while Cream performed best at 8 hours exposure time. Fast neutron was observed to improve the performance of Bambara groundnut, thus can be used in breeding programme for Bambara groundnut.


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