Total and double-stranded DNA-specific immunoglobulin E in bronchoalveolar lavage fluid of children with human adenovirus pneumonia

Abstract Menispermum dauricum is widely used to treat respiratory inflammation, including laryngopharyngitis, tonsillitis, tracheitis, and bronchitis. Total alkaloids isolated from M. dauricum have shown a variety of beneficial bioactivities. However, available data on the effects of M. dauricum total alkaloids against allergic asthma has not been reported. In present study, the protective effect of M. dauricum total alkaloids was evaluated by using an ovalbumin-induced in vivo model of asthma. The asthma model was prepared by sensitizing and challenging mice with ovalbumin, and M. dauricum total alkaloids (100, 200, and 400 mg/kg) were administrated to asthmatic mice by gavage. Histopathological analysis of pulmonary changes was detected by hematoxylin and eosin, and periodic acid-schiff staining. Inflammatory cell counts were determined in bronchoalveolar lavage fluid. Total immunoglobulin E and ovalbumin-specific immunoglobulin E levels in serum, and T-helper 2 cytokines and chemokine levels in bronchoalveolar lavage fluid were detected by an ELISA. Histological results demonstrated that M. dauricum total alkaloids significantly attenuated pulmonary inflammation in asthmatic mice. M. dauricum total alkaloid treatment exhibited marked effects on asthmatic mice in reducing inflammatory cell counts, decreasing interleukin-4, interleukin-5, and interleukin-13 concentrations, and downregulating TNF-α and eotaxin levels in bronchoalveolar lavage fluid. In addition, M. dauricum total alkaloids could also inhibit the elevated serum levels of total immunoglobulin E and ovalbumin-specific immunoglobulin E. These findings confirmed that M. dauricum total alkaloids could suppress airway inflammation in ovalbumin-induced asthma through regulating the T-helper 2 response and chemokine level. M. dauricum total alkaloids may be a potential ethnopharmacological agent for asthmatic patients.

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Paeonol attenuates airway inflammation and hyperresponsiveness in a murine model of ovalbumin-induced asthma

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y10-077 ◽

2010 ◽

Vol 88 (10) ◽

pp. 1010-1016 ◽

Cited By ~ 20

Author(s):

Qiang Du ◽

Gan-Zhu Feng ◽

Li Shen ◽

Jin Cui ◽

Jian-Kang Cai

Keyword(s):

Bronchoalveolar Lavage ◽

Airway Inflammation ◽

Bronchoalveolar Lavage Fluid ◽

Immunoglobulin E ◽

Therapeutic Potential ◽

Lavage Fluid ◽

Interleukin 4 ◽

Moutan Cortex ◽

Ifn Γ ◽

Anti Inflammatory

Paeonol, the main active component isolated from Moutan Cortex, possesses extensive pharmacological activities such as anti-inflammatory, anti-allergic, and immunoregulatory effects. In the present study, we examined the effects of paeonol on airway inflammation and hyperresponsiveness in a mouse model of allergic asthma. BALB/c mice sensitized and challenged with ovalbumin were administered paeonol intragastrically at a dose of 100 mg/kg daily. Paeonol significantly suppressed ovalbumin-induced airway hyperresponsiveness to acetylcholine chloride. Paeonol administration significantly inhibited the total inflammatory cell and eosinophil count in bronchoalveolar lavage fluid. Treatment with paeonol significantly enhanced IFN-γ levels and decreased interleukin-4 and interleukin-13 levels in bronchoalveolar lavage fluid and total immunoglobulin E levels in serum. Histological examination of lung tissue demonstrated that paeonol significantly attenuated allergen-induced lung eosinophilic inflammation and mucus-producing goblet cells in the airway. These data suggest that paeonol exhibits anti-inflammatory activity in allergic mice and may possess new therapeutic potential for the treatment of allergic bronchial asthma.

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Frank elevation of bronchoalveolar lavage fluid IgE level in hyperimmunoglobulin E syndrome

Chest Disease Reports ◽

10.4081/cdr.2011.e4 ◽

2011 ◽

Vol 1 (1) ◽

pp. 4

Author(s):

Alan Altraja

Keyword(s):

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Immunoglobulin E ◽

Elevated Serum ◽

Lavage Fluid ◽

Infectious Episode ◽

Hyperimmunoglobulin E Syndrome ◽

Immunodeficiency Disorder ◽

Respiratory Membrane ◽

Cellular Components

Hyperimmunoglobulin E syndrome (HIES) is a rare hereditary immunodeficiency disorder characterized mainly by an elevated serum immunoglobulin E (IgE) level. However, the IgE level in bronchoalveolar lavage fluid (BALF) of HIES patients has not been addressed before. The case of a 24-year-old non-smoking Caucasian male HIES patient with classical skin infections in childhood and recurrent staphylococcal pneumonias is reported. Out of an infectious episode, the total IgE level was measured in BALF by chemiluminescence method, along with appropriate analysis of immunoglobulins A (IgA), G (IgG) and M (IgM), albumin and cellular components. In parallel, the respective markers were measured in serum. An elevated level of BALF IgE of 15.10 ng/mL (6.24 IU/mL) along with the marked heightening of IgE in serum at 130,583 ng/mL (53,960 IU/mL) was observed. The relative coefficients of excretion (RCE), based on BALF and serum albumin concentrations (100.6 mg/L and 39 g/L, respectively), were 0.0448, 1.49, 1.94 and <3.40 for IgE, IgA, IgG and IgM, respectively. The results show a frank increase in BALF IgE level in HIES compared to that reported for healthy individuals and the low RCE value for IgE suggest a diffusion of IgE across the respiratory membrane. This case study demonstrates a markedly elevated BALF IgE level in HIES that could be used to support the diagnosis, but also raises important BALFrelated aspects in HIES that provoke further studies on BALF from HIES patients.

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Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in Pneumonia

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2020/6839103 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Xu Chen ◽

Shuizi Ding ◽

Cheng Lei ◽

Jieli Qin ◽

Ting Guo ◽

...

Keyword(s):

Next Generation Sequencing ◽

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Human Adenovirus ◽

Lavage Fluid ◽

Diagnostic Value ◽

Next Generation ◽

Barr Virus ◽

Bacteria And Fungi ◽

Generation Sequencing

Background. Metagenomic next-generation sequencing (mNGS) has made a revolution in the mode of pathogen identification. We decided to explore the diagnostic value of blood and bronchoalveolar lavage fluid (BALF) as mNGS samples in pneumonia. Methods. We retrospectively reviewed 467 mNGS results and assessed the diagnostic performance of paired blood and BALF mNGS in 39 patients with pneumonia. Results. For bacteria and fungi, 16 patients had culture-confirmed pathogen diagnosis, while 13 patients were culture-negative. BALF mNGS was more sensitive than blood mNGS (81.3% vs. 25.0%, p=0.003), and the specificity in BALF and blood mNGS was not statistically significant different (76.9% vs. 84.6%, p=0.317). For 10 patients without culture test, treatments were changed in 2 patients. For viruses, Epstein-Barr virus was positive in blood mNGS in 9 patients. Human adenovirus was detected in both BALF and blood mNGS in 3 patients. Conclusion. Our study suggests that BALF mNGS is more sensitive than blood mNGS in detecting bacteria and fungi, but blood also has advantages to identify the pathogens of pneumonia, especially for some viruses.

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