Protective Effects of Total Alkaloids from Menispermum dauricum against Airway Inflammation in Asthmatic Mice

Planta Medica ◽  
2020 ◽  
Vol 86 (10) ◽  
pp. 665-673
Author(s):  
Le Lv ◽  
Bing Yin ◽  
Ye You ◽  
Zhongyang Sun ◽  
Jiao He ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Immunoglobulin E ◽  
Lavage Fluid ◽  
T Helper ◽  
T Helper 2 ◽  
Total Alkaloids ◽  
Cell Counts ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

Abstract Menispermum dauricum is widely used to treat respiratory inflammation, including laryngopharyngitis, tonsillitis, tracheitis, and bronchitis. Total alkaloids isolated from M. dauricum have shown a variety of beneficial bioactivities. However, available data on the effects of M. dauricum total alkaloids against allergic asthma has not been reported. In present study, the protective effect of M. dauricum total alkaloids was evaluated by using an ovalbumin-induced in vivo model of asthma. The asthma model was prepared by sensitizing and challenging mice with ovalbumin, and M. dauricum total alkaloids (100, 200, and 400 mg/kg) were administrated to asthmatic mice by gavage. Histopathological analysis of pulmonary changes was detected by hematoxylin and eosin, and periodic acid-schiff staining. Inflammatory cell counts were determined in bronchoalveolar lavage fluid. Total immunoglobulin E and ovalbumin-specific immunoglobulin E levels in serum, and T-helper 2 cytokines and chemokine levels in bronchoalveolar lavage fluid were detected by an ELISA. Histological results demonstrated that M. dauricum total alkaloids significantly attenuated pulmonary inflammation in asthmatic mice. M. dauricum total alkaloid treatment exhibited marked effects on asthmatic mice in reducing inflammatory cell counts, decreasing interleukin-4, interleukin-5, and interleukin-13 concentrations, and downregulating TNF-α and eotaxin levels in bronchoalveolar lavage fluid. In addition, M. dauricum total alkaloids could also inhibit the elevated serum levels of total immunoglobulin E and ovalbumin-specific immunoglobulin E. These findings confirmed that M. dauricum total alkaloids could suppress airway inflammation in ovalbumin-induced asthma through regulating the T-helper 2 response and chemokine level. M. dauricum total alkaloids may be a potential ethnopharmacological agent for asthmatic patients.

Paeonol attenuates airway inflammation and hyperresponsiveness in a murine model of ovalbumin-induced asthma

2010 ◽  
Vol 88 (10) ◽  
pp. 1010-1016 ◽  
Author(s):  
Qiang Du ◽  
Gan-Zhu Feng ◽  
Li Shen ◽  
Jin Cui ◽  
Jian-Kang Cai
Keyword(s):  
Bronchoalveolar Lavage ◽  
Airway Inflammation ◽  
Bronchoalveolar Lavage Fluid ◽  
Immunoglobulin E ◽  
Therapeutic Potential ◽  
Lavage Fluid ◽  
Interleukin 4 ◽  
Moutan Cortex ◽  
Ifn Γ ◽  
Anti Inflammatory

Paeonol, the main active component isolated from Moutan Cortex, possesses extensive pharmacological activities such as anti-inflammatory, anti-allergic, and immunoregulatory effects. In the present study, we examined the effects of paeonol on airway inflammation and hyperresponsiveness in a mouse model of allergic asthma. BALB/c mice sensitized and challenged with ovalbumin were administered paeonol intragastrically at a dose of 100 mg/kg daily. Paeonol significantly suppressed ovalbumin-induced airway hyperresponsiveness to acetylcholine chloride. Paeonol administration significantly inhibited the total inflammatory cell and eosinophil count in bronchoalveolar lavage fluid. Treatment with paeonol significantly enhanced IFN-γ levels and decreased interleukin-4 and interleukin-13 levels in bronchoalveolar lavage fluid and total immunoglobulin E levels in serum. Histological examination of lung tissue demonstrated that paeonol significantly attenuated allergen-induced lung eosinophilic inflammation and mucus-producing goblet cells in the airway. These data suggest that paeonol exhibits anti-inflammatory activity in allergic mice and may possess new therapeutic potential for the treatment of allergic bronchial asthma.

scholarly journals Bronchoalveolar lavage fluid cell counts in cryptogenic fibrosing alveolitis and their relation to therapy.

Thorax ◽  
1980 ◽  
Vol 35 (5) ◽  
pp. 328-339 ◽  
Author(s):  
P L Haslam ◽  
C W Turton ◽  
A Lukoszek ◽  
A J Salsbury ◽  
A Dewar ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Fibrosing Alveolitis ◽  
Cell Counts ◽  
Cryptogenic Fibrosing Alveolitis ◽  
Fluid Cell

scholarly journals The Fermented Soy Product ImmuBalanceTM Suppresses Airway Inflammation in a Murine Model of Asthma

Nutrients ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 3380
Author(s):  
Hideaki Kadotani ◽  
Kazuhisa Asai ◽  
Atsushi Miyamoto ◽  
Kohei Iwasaki ◽  
Takahiro Kawai ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Airway Inflammation ◽  
Murine Model ◽  
Bronchoalveolar Lavage Fluid ◽  
Inflammatory Cell ◽  
Allergic Airway Inflammation ◽  
Lavage Fluid ◽  
Cell Infiltration ◽  
Mucus Production ◽  
Cell Counts

The fermented soy product ImmuBalance contains many active ingredients and its beneficial effects on some allergic diseases have been reported. We hypothesized that ImmuBalance could have potential effects on airway inflammation in a murine model of asthma. Mice sensitized and challenged with ovalbumin developed airway inflammation. Bronchoalveolar lavage fluid was assessed for inflammatory cell counts and levels of cytokines. Lung tissues were examined for cell infiltration and mucus hypersecretion. Oral administration of ImmuBalance significantly inhibited ovalbumin-induced eosinophilic inflammation and decreased Th2 cytokine levels in bronchoalveolar lavage fluid (p < 0.05). In addition, lung histological analysis showed that ImmuBalance inhibited inflammatory cell infiltration and airway mucus production. Our findings suggest that supplementation with ImmuBalance may provide a novel strategy for the prevention or treatment of allergic airway inflammation.

scholarly journals Frank elevation of bronchoalveolar lavage fluid IgE level in hyperimmunoglobulin E syndrome

2011 ◽  
Vol 1 (1) ◽  
pp. 4
Author(s):  
Alan Altraja
Keyword(s):  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Immunoglobulin E ◽  
Elevated Serum ◽  
Lavage Fluid ◽  
Infectious Episode ◽  
Hyperimmunoglobulin E Syndrome ◽  
Immunodeficiency Disorder ◽  
Respiratory Membrane ◽  
Cellular Components

Hyperimmunoglobulin E syndrome (HIES) is a rare hereditary immunodeficiency disorder characterized mainly by an elevated serum immunoglobulin E (IgE) level. However, the IgE level in bronchoalveolar lavage fluid (BALF) of HIES patients has not been addressed before. The case of a 24-year-old non-smoking Caucasian male HIES patient with classical skin infections in childhood and recurrent staphylococcal pneumonias is reported. Out of an infectious episode, the total IgE level was measured in BALF by chemiluminescence method, along with appropriate analysis of immunoglobulins A (IgA), G (IgG) and M (IgM), albumin and cellular components. In parallel, the respective markers were measured in serum. An elevated level of BALF IgE of 15.10 ng/mL (6.24 IU/mL) along with the marked heightening of IgE in serum at 130,583 ng/mL (53,960 IU/mL) was observed. The relative coefficients of excretion (RCE), based on BALF and serum albumin concentrations (100.6 mg/L and 39 g/L, respectively), were 0.0448, 1.49, 1.94 and &lt;3.40 for IgE, IgA, IgG and IgM, respectively. The results show a frank increase in BALF IgE level in HIES compared to that reported for healthy individuals and the low RCE value for IgE suggest a diffusion of IgE across the respiratory membrane. This case study demonstrates a markedly elevated BALF IgE level in HIES that could be used to support the diagnosis, but also raises important BALFrelated aspects in HIES that provoke further studies on BALF from HIES patients.

scholarly journals Interstitial Score and Concentrations of IL-4Rα, PAR-2, and MMP-7 in Bronchoalveolar Lavage Fluid Could Be Useful Markers for Distinguishing Idiopathic Interstitial Pneumonias

Diagnostics ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 693
Author(s):  
Magdalena Bruzova ◽  
Martina Pavlova ◽  
Radoslav Matej ◽  
Martina Sterclova ◽  
Martina Vasakova
Keyword(s):  
Differential Diagnosis ◽  
Bronchoalveolar Lavage ◽  
Interstitial Pneumonia ◽  
Lung Diseases ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Idiopathic Interstitial Pneumonia ◽  
Unknown Etiology ◽  
Cell Counts ◽  
Tnf Α

Idiopathic interstitial pneumonia (IIP) entails a variable group of lung diseases of unknown etiology. Idiopathic pulmonary fibrosis, nonspecific interstitial pneumonia, interstitial lung diseases related to connective tissue disease (CTD-ILD), and hypersensitivity pneumonitis (HP) can manifest with similar clinical, radiological, and histopathological features. In a differential diagnosis, biomarkers can play a significant role. We assume that levels of specific cyto- or chemokines or their receptors can signal pathogenetic processes in the lungs. Eighty patients with different types of idiopathic interstitial pneumonia were enrolled in this study. Cell counts and concentrations of tumor necrosis factor (TNF)-α, interleukin-4 receptor α, proteinase-activated receptor (PAR)-2, matrix metalloproteinase (MMP)-7, and B cell-activating factor were measured in bronchoalveolar lavage fluid using commercial ELISA kits. High resolution computer tomography results were evaluated using alveolar and interstitial (IS) score scales. Levels of TNF-α were significantly higher in HP compared to fibrosing IIP (p < 0.0001) and CTD-ILD (p = 0.0381). Concentrations of IL-4Rα, PAR-2, and MMP-7 were positively correlated with IS (p = 0.0009; p = 0.0256; p = 0.0015, respectively). Since TNF-α plays a major role in inflammation, our results suggest that HP is predominantly an inflammatory disease. From the positive correlation with IS we believe that IL-4Rα, PAR-2, and MMP-7 could serve as fibroproliferative biomarkers in differential diagnosis of IIP.

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