Rapid identification and quantification of bioactive metabolites in processed Camellia sinensis samples by UHPLC-ESI-MS/MS and evaluation of their antioxidant activity

Non-conventional extraction of bioactive metabolites could provide sustainable alternative techniques to preserve the potency of antioxidants and antiviral compounds extracted from macro-algae. In this paper, we first reviewed the antioxidant and antiviral potential of the active metabolites that exist in the three known macro-algae classes; Phaeophyceae, Rhodophyceae, and Chlorophyceae, and a comparison between their activities is discussed. Secondly, a review of conventional and non-conventional extraction methods is undertaken. The review then focused on identifying the optimal extraction method of sulphated polysaccharide from macro-algae that exhibits both antiviral and antioxidant activity. The review finds that species belonging to the Phaeophyceae and Rhodophceae classes are primarily potent against herpes simplex virus, followed by human immunodeficiency virus and influenza virus. At the same time, species belonging to Chlorophyceae class are recorded by most of the scholars to have antiviral activity against herpes simplex virus 1. Additionally, all three macro-algae classes exhibit antioxidant activity, the potency of which is a factor of the molecular structure of the bioactive metabolite as well as the extraction method applied.

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COMPARATIVE ANALYSIS OF MORPHOLOGY AND PHYTOCHEMICAL CONSTITUENTS IN DIFFERENT POPULATIONS AND MORPHOTYPES OF DATURA INNOXIA MILL. AND DATURA METEL L. FROM PUNJAB PLAINS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i1.27720 ◽

2019 ◽

Vol 12 (1) ◽

pp. 193 ◽

Cited By ~ 2

Author(s):

Mahinder Partap ◽

Raghbir Chand Gupta ◽

Saroj Kumar Pradhan

Keyword(s):

Antioxidant Activity ◽

Comparative Analysis ◽

Chlorogenic Acid ◽

Caffeic Acid ◽

Health Benefit ◽

Bioactive Metabolites ◽

Datura Innoxia ◽

Datura Metel ◽

Different Populations ◽

Phytochemical Constituents

Objective: Comparative analysis of morphology and phytochemical constituents in different populations and morphotypes of Datura innoxia Mill. and Datura metel L. from Punjab plains.Methods: Morphological analyses of different populations have been done. Methanol extracts of seeds and roots of different populations have been prepared and total phenols, flavonoid contents were measured through spectrophotometry. Antioxidant activity was studied by 2, 2-diphenyl-1- picrylhydrazyl radical scavenging activity and total antioxidant capacity. Two major compounds, caffeic acid and chlorogenic acid were quantified by high-performance thin-layer chromatography (HPTLC) analyses.Results: Two morphotypes of D. metel were reported in the present study. Out of five different populations of D. metel and D. innoxia, the wild populations have more bioactive compound as compared to the cultivated ones from the detailed phytochemical investigation. Pharmacologically important two marker compounds chlorogenic acid and caffeic acid has been identified and quantified by HPTLC technique.Conclusion: Variation in terms of morphology and secondary metabolites exists among the different populations of Datura spp. Among the two plant parts studied, seeds have the maximum amount of bioactive metabolites and antioxidant activity. This study revealed that chlorogenic acid and caffeic acid are the potential polyphenolic compounds in Datura spp. It has been found that the antioxidant activity of plant is due to its polyphenol contents, which provides insight to various researchers to work on it as it imparts health benefit.

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Identification and quantification of constituents of Gardenia jasminoides Ellis (Zhizi) by HPLC-DAD–ESI–MS

Food Chemistry ◽

10.1016/j.foodchem.2012.02.157 ◽

2012 ◽

Vol 134 (2) ◽

pp. 1199-1204 ◽

Cited By ~ 39

Author(s):

M.C. Bergonzi ◽

C. Righeschi ◽

B. Isacchi ◽

A.R. Bilia

Keyword(s):

Esi Ms ◽

Gardenia Jasminoides ◽

Gardenia Jasminoides Ellis ◽

Identification And Quantification

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Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

Brazilian Journal of Biology ◽

10.1590/1519-6984.182959 ◽

2019 ◽

Vol 79 (3) ◽

pp. 452-459 ◽

Cited By ~ 15

Author(s):

F. R. G. Silva ◽

T. M. S. Matias ◽

L. I. O. Souza ◽

T. J. Matos-Rocha ◽

S. A. Fonseca ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Phenolic Compounds ◽

Gallic Acid ◽

Bioactive Metabolites ◽

Diffusion Method ◽

Total Phenolic ◽

Phytochemical Screening ◽

Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

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