The extracellular constitutive production of chitin deacetylase in Metarhizium anisopliae: possible edge to entomopathogenic fungi in the biological control of insect pests

2004 ◽  
Vol 85 (2) ◽  
pp. 80-88 ◽  
Author(s):  
Pallavi Nahar ◽  
Vandana Ghormade ◽  
Mukund V Deshpande
Keyword(s):  
Biological Control ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Chitin Deacetylase ◽  
Constitutive Production

scholarly journals Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of South Sumatra, Indonesia

2018 ◽  
Vol 19 (6) ◽  
pp. 2365-2373 ◽  
Author(s):  
AYU SAFITRI ◽  
SITI HERLINDA ◽  
ARUM SETIAWAN
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Vegetation Type ◽  
Tenebrio Molitor ◽  
Soil Samples ◽  
Fungal Isolates

Safitri A, Herlinda S, Setiawan A. 2018. Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of South Sumatra, Indonesia. Biodiversitas 19: 2365-2373. Ecosystems of lowlands and highlands in South Sumatra have specific characteristics of soils and vegetations that can affect the availability of entomopathogenic fungi. This study aimed to explore and identify species and to determine inoculum potentials of the entomopathogenic fungi from soils of freshwater swamps, tidal lowlands, peatlands, and highlands. Baiting of entomopathogenic fungi on soil samples used the larvae of Tenebrio molitor. The entomopathogenic fungi species found in this research were Beauveria bassiana and Metarhizium anisopliae. The number of the fungal isolates were 30 isolates consisting of nine isolates of B. bassiana and 21 isolates of M. anisopliae.The highest number of isolates was found in the highland ecosystem (11 isolates) and the lowest was found in peatland ecosystem (4 isolates). The highest percentage average of inoculum potentials of the fungi was found in the high land ecosystem (4.04%) and the lowest one was found in freshwater swamps ecosystem (2.11%). Based on the vegetation type, the soil planted with mustard in Talang Patai-Pagaralam (highland ecosystem) had the highest inoculum potentials (9.33%). These fungi will make an important contribution to the biological control for insect pests in lowland to highland ecosystems in Indonesia.

scholarly journals Evaluation of cellulose substrates treated with Metarhizium anisopliae (Metschnikoff) Sorokin as a biological control agent against the termite Microcerotermes diversus Silvestri (Isoptera: Termitidae)

2011 ◽  
Vol 43 (2) ◽  
pp. 269
Author(s):  
Behzad Habibpour ◽  
Amir Cheraghi ◽  
Mohammad Saeed Mossadegh
Keyword(s):  
Biological Control ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Biological Control Agent ◽  
Control Agent ◽  
Cellulose Substrates ◽  
Virulent Strains ◽  
Sustainable Protection ◽  
Chemical Pesticides

This article is the first report on the promising effect of an entomopathogenic fungus, <em>Metarhizium anisopliae</em> (Metschnikoff) Sorokin to control populations of <em>Microcerotermes diversus </em>Silvestri. Biological control is an alternative to the long-term usage of chemical pesticides.<em> M. anisopliae</em>, the causal agent of green muscardine disease of insects, is an important fungus in biological control of insect pests. Bait systems can eliminate entire colonies of subterranean termites. Baiting reduces adverse environmental impacts caused by organochlorine and organophosphate pesticides in the control of termites and creates sustainable protection of buildings against their invasion. Treated-sawdust bait was applied by two methods: a) combination of treated sawdust and untreated filter paper, and b) combination of treated sawdust and untreated sawdust. When combinations of treated sawdust and untreated sawdust were used, LC50 and LC90 were 8.4&times;106 and 3.9&times;107 (spore/ml), respectively. With the use of improved bait formula and more virulent strains, we hope to achieve better control of termite colonies and enable pathogens to become a useful element in the Integrated Pest Management system.

Different strategies to kill the host presented byMetarhizium anisopliaeandBeauveria bassiana

2018 ◽  
Vol 64 (3) ◽  
pp. 191-200 ◽  
Author(s):  
Cynthia Barbosa Rustiguel ◽  
María Fernández-Bravo ◽  
Luis Henrique Souza Guimarães ◽  
Enrique Quesada-Moraga
Keyword(s):  
Secondary Metabolites ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Galleria Mellonella ◽  
Insect Pests ◽  
Fungal Growth ◽  
Growth Strategy

Studies conducted over the last decades have shown the potential of entomopathogenic fungi for the biocontrol of some insect pests. Entomopathogenic fungi infect their host through the cuticle, so they do not need to be ingested to be effective. These fungi also secrete secondary metabolites and proteins that are toxic to insect pests. In this context, we analyzed the pathogenicity of Metarhizium anisopliae (Metschn.) strains IBCB 384 and IBCB 425 and Beauveria bassiana (Bals.-Criv.) Vuill. strains E 1764 and E 3158 against Galleria mellonella (Linn.) larvae, during pre-invasion and post-invasion phases. The results showed M. anisopliae, especially strain IBCB 384, was most virulent in the pre-invasion phase against G. mellonella, whereas B. bassiana, especially strain E 1764, was most virulent in the post-invasion phase. During in vivo development and in the production of toxic serum, B. bassiana E 3158 was the most virulent. Different fungal growth (or toxin) strategies were observed for studied strains. Metarhizium anisopliae IBCB 425 prioritizes the growth strategy, whereas strain IBCB 384 and B. bassiana strains E 1764 and E 3158 have a toxic strategy. All strains have pathogenicity against G. mellonella, indicating their possible use for biocontrol.

scholarly journals KOMPATIBILITAS CENDAWAN METARHIZIUM ANISOPLIAE (METSCHN.) SOROKIN DENGAN NEMATODA ENTOMOPATOGEN STEINERNEMA SP.

2017 ◽  
Vol 17 (2) ◽  
pp. 111
Author(s):  
Imron Rosyidi ◽  
Hari Purnomo ◽  
Nanang Tri Haryadi ◽  
Mohammad Hoesain
Keyword(s):  
Biological Control ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Entomopathogenic Nematode ◽  
Research Result ◽  
Symbiotic Bacteria ◽  
Randomized Design ◽  
Completely Randomized Design

Compatibility Metarhizium anisopliae (Metschn.) Sorokin with entomopathogenic nematode Steinernema sp. Metarhizium anisopliae (Metschn.) Sorokin and entomopathogenic nematode Steinernema sp. can be used as biological control of insect pests. The objective of this research was to identify compatibility between fungus M. anisopliae with entomopathogenic nematode Steinernema sp. This research was conducted using a completely randomized design (CRD) consisting of 5 treatments and repeated 5 times. The treatment used was M. anisopliae isolates Jombang 1 with Steinernema sp. (M1), M. anisopliae Jombang 2 with Steinernema sp. (M2), M. anisopliae isolates Kediri with Steinernema sp. (M3), M. anisopliae isolates Bondowoso with Steinernema sp. (M4), and M. anisopliae isolates Banyuwangi with Steinernema sp. (M5). Based on this research result, compatibility between the fungus M. anisopliae with entomopathogenic nematode Steinernema sp. was antagonistic. This occurs due to the symbiotic bacteria Xenorhabdus sp. that produced compounds antimycotic and capable to inhibit the growth of fungus M. anisopliae.

scholarly journals Effects of the Entomopathogenic Fungus Metarhizium anisopliae on the Mortality and Immune Response of Locusta migratoria

Insects ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 36 ◽  
Author(s):  
Wuji Jiang ◽  
Yifan Peng ◽  
Jiayi Ye ◽  
Yiyi Wen ◽  
Gexin Liu ◽  
...  
Keyword(s):  
Immune Response ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Insect Pests ◽  
Locusta Migratoria ◽  
Management Strategies ◽  
Sod Activity ◽  
Immune Related Genes ◽  
Locusta Migratoria Manilensis

Entomopathogenic fungi are the key regulators of insect populations and some of them are important biological agents used in integrated pest management strategies. Compared with their ability to become resistant to insecticides, insect pests do not easily become resistant to the infection by entomopathogenic fungi. In this study, we evaluated the mortality and immune response of the serious crop pest Locusta migratoria manilensis after exposure to a new entomopathogenic fungus strain, Metarhizium anisopliae CQMa421. M. anisopliae CQMa421 could effectively infect and kill the L. migratoria adults and nymphs. The locust LT50 under 1 × 108 conidia/mL concentration of M. anisopliae was much lower than that under conidial concentration 1 × 105 conidia/mL (i.e., 6.0 vs. 11.2 and 5.0 vs. 13.8 for adults and nymphs, respectively). The LC50 (log10) of M. anisopliae against locust adults and nymphs after 10 days was 5.2 and 5.6, respectively. Although the number of hemocytes in L. migratoria after exposure to M. anisopliae did not differ with that in the controls, the enzymatic activity of superoxide dismutase (SOD) and prophenoloxidase (ProPO) did differ between the two treatments. The activities of both SOD and ProPO under the M. anisopliae treatment were lower than that in the controls, except for the ProPO activity at 72 h and the SOD activity at 96 h. Further, the expression of the L. migratoria immune-related genes defensin, spaetzle, and attacin differed after exposure to M. anisopliae for 24 h to 96 h. Taken together, this study indicated that infection with M. anisopliae CQMa421 could cause the death of L. migratoria by interacting with the immune responses of the host, demonstrating that this fungal strain of M. anisopliae can be an efficient biocontrol agent against L. migratoria.

Entomopathogenic Fungi: A Potential Source for Biological Control of Insect Pests

2020 ◽  
pp. 225-250
Author(s):  
Anjney Sharma ◽  
Ankit Srivastava ◽  
Awadhesh K. Shukla ◽  
Kirti Srivastava ◽  
Alok Kumar Srivastava ◽  
...  
Keyword(s):  
Biological Control ◽  
Entomopathogenic Fungi ◽  
Insect Pests ◽  
Potential Source

scholarly journals Application of Bait Treated with the Entomopathogenic FungusMetarhizium anisopliae(Metsch.) Sorokin for the Control ofMicrocerotermes diversusSilv.

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Amir Cheraghi ◽  
Behzad Habibpour ◽  
Mohammad Saied Mossadegh
Keyword(s):  
Biological Control ◽  
Pest Management ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Fungal Pathogen ◽  
Management Strategy ◽  
Khuzestan Province ◽  
Significant Difference ◽  
Strategy Performance ◽  
Bait System

Microcerotermes diversusSilvestri (Isoptera, Termitidae) is considered to be the most destructive termite in Khuzestan province (Iran), and its control by conventional methods is often difficult. Biological control using entomopathogenic fungi could be an alternative management strategy. Performance of a bait matrix treated with the entomopathogenic fungusMetarhizium anisopliae(Metsch.) Sorokin, Strain Saravan (DEMI 001), againstM. diversuswas evaluated in this paper. The highest rate of mortality occurred at concentrations of 3.7 × 107and 3.5 × 108(conidia per mL). There was no significant difference between treatments, in the rate of feeding on the bait. The fungal pathogen was not repellent to the target termite over the conidial concentrations used. The current results suggest potential of such bait system in controlling termite. However the effectiveness ofM. anisopliaeas a component of integrated pest management forM. diversusstill needs to be proven under field conditions.

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