scholarly journals KOMPATIBILITAS CENDAWAN METARHIZIUM ANISOPLIAE (METSCHN.) SOROKIN DENGAN NEMATODA ENTOMOPATOGEN STEINERNEMA SP.

2017 ◽  
Vol 17 (2) ◽  
pp. 111
Author(s):  
Imron Rosyidi ◽  
Hari Purnomo ◽  
Nanang Tri Haryadi ◽  
Mohammad Hoesain
Keyword(s):  
Biological Control ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Entomopathogenic Nematode ◽  
Research Result ◽  
Symbiotic Bacteria ◽  
Randomized Design ◽  
Completely Randomized Design

Compatibility Metarhizium anisopliae (Metschn.) Sorokin with entomopathogenic nematode Steinernema sp. Metarhizium anisopliae (Metschn.) Sorokin and entomopathogenic nematode Steinernema sp. can be used as biological control of insect pests. The objective of this research was to identify compatibility between fungus M. anisopliae with entomopathogenic nematode Steinernema sp. This research was conducted using a completely randomized design (CRD) consisting of 5 treatments and repeated 5 times. The treatment used was M. anisopliae isolates Jombang 1 with Steinernema sp. (M1), M. anisopliae Jombang 2 with Steinernema sp. (M2), M. anisopliae isolates Kediri with Steinernema sp. (M3), M. anisopliae isolates Bondowoso with Steinernema sp. (M4), and M. anisopliae isolates Banyuwangi with Steinernema sp. (M5). Based on this research result, compatibility between the fungus M. anisopliae with entomopathogenic nematode Steinernema sp. was antagonistic. This occurs due to the symbiotic bacteria Xenorhabdus sp. that produced compounds antimycotic and capable to inhibit the growth of fungus M. anisopliae.

scholarly journals Activity of strains of Metarhizium anisopliae (Metsch) Sorokin against white grub (Chiloloba acuta, Scarabaeidae, Coleoptera) under laboratory condition in Nepal

2018 ◽  
Vol 17 ◽  
pp. 82-91 ◽  
Author(s):  
Dipak Khanal
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Microbial Control ◽  
White Grub ◽  
White Grubs ◽  
Larval Stages ◽  
Commercial Strain ◽  
Randomized Design ◽  
Completely Randomized Design

Soil insect pests are the major productivity constrains of different crops among which white grubs (Coleoptera: Scarabaeidae), both adult and larval stages, are extremely destructive in nature. Laboratory studies were conducted to evaluate the virulence of an indigenous and a commercial strain of the entomopathogenic fungi, Metarhizium anisopliae (Metsch.) Sorokin, against white grubs species Chiloloba acuta by applying the dipping method at Entomology Division, NARC, Nepal. Third instars larvae of C. acuta were dipped in suspensions of indigenous and commercial strains (Pacer) of M. anisopliae at different concentrations ranging from 3.33×104 to 1.04×108 spores/ml for 3-5 seconds which resulted in 97.8% and 89% mortalities with the highest dose of 1.04×108 spores/ml 40 days after inoculation (DAI), respectively. The LC50 values for indigenous and commercial strain were 3.5×105 and 1.88×106 spores/ml, respectively, with the potency of 1:0.28 at 40 DAI. Bioassays were conducted in completely randomized design. From results it is concluded that the tested strains of entomopathogenic fungi have potential as microbial control agents in managing white grubs in laboratory and it is suggested to be tested under farmers field condition.

scholarly journals Characterization of symbiotic and associated bacteria from entomopathogenic nematode Heterorhabditis sp. (nematode: Heterorhabditidae) isolated from India

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Rashid Pervez ◽  
Showkat Ahmad Lone ◽  
Sasmita Pattnaik
Keyword(s):  
Insect Pests ◽  
Entomopathogenic Nematode ◽  
Alcaligenes Faecalis ◽  
Symbiotic Bacteria ◽  
Rrna Gene ◽  
Main Body ◽  
Associated Bacteria ◽  
Molecular Approaches

Abstract Background Entomopathogenic nematodes (EPNs) harboring symbiotic bacteria are one of the safest alternatives to the chemical insecticides for the control of various insect pests. Infective juveniles of EPNs locate a target insect, enter through the openings, and reach the hemocoel, where they release the symbiotic bacteria and the target gets killed by the virulence factors of the bacteria. Photorhabdus with Heterorhabditis spp. are well documented; little is known about the associated bacteria. Main body In this study, we explored the presence of symbiotic and associated bacteria from Heterorhabditis sp. (IISR-EPN 09) and characterized by phenotypic, biochemical, and molecular approaches. Six bacterial isolates, belonging to four different genera, were recovered and identified as follows: Photorhabdus luminescens, one each strain of Providencia vermicola, Pseudomonas entomophila, Alcaligenes aquatilis, and two strains of Alcaligenes faecalis based on the phenotypic, biochemical criteria and the sequencing of 16S rRNA gene. Conclusion P. luminescens is symbiotically associated with Heterorhabditis sp. (IISR-EPN 09), whereas P. vermicola, P. entomophila, A. aquatilis, and A. faecalis are the associated bacteria. Further studies are needed to determine the exact role of the bacterial associates with the Heterorhabditis sp.

scholarly journals PENGARUH EKSTRAK DAUN KUNYIT (Curcuma longa Linn.) SEBAGAI INSEKTISIDA ELEKTRIK TERHADAP MORTALITAS NYAMUK Culex sp. L.

Pro-Life ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 44
Author(s):  
Aseptianova Aseptianova
Keyword(s):  
Disease Prevention ◽  
Toxicity Test ◽  
Curcuma Longa ◽  
Research Result ◽  
Probit Analysis ◽  
Turmeric Extract ◽  
Natural Insecticide ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Culex Sp

Mosquito Culex sp. L. is a vector of elephantiasis disease, prevention effort of elephantiasis vector is usually done by using chemical and natural insecticide. In the leaves of turmeric can be used as a natural insecticide in the prevention of elephantiasis vector disease. The objectives of this research are (1) to know the effect of turmeric extract ((Curcuma longa Linn.) As electrical insecticide to mortality of Culex sp. L. (2) To know the concentration of turmeric extract (Curcuma longa Linn.) Which is effective to be used as an electric insecticide against mortality of Culex sp. L. expressed by the toxicity test of LC50 and LT50. The method used in this research is an experimental method with Completely Randomized Design (RAL) which consists of 5 treatments and 4 replications. Based on research result of turmeric extract (Curcuma longa Linn.) Have a very significant effect on mortality of Culex sp. L. with sig value 0,000 <sig value. 0.05. The probit analysis of LC50 of turmeric leaves extract on mortality of Culex sp. L. is 44, 840 ml, meaning that at that concentration has occurred 50% mortality of total mosquito. The probit analysis of LT50 turmeric leaves extract on mortality of Culex sp. L. for 1.825 hours.   Keywords: turmeric leaves extract, mortality Culex sp. L. mosquito

scholarly journals Selectivity of pesticides registered for soybean crop on Telenomus podisi and Trissolcus basalis

2018 ◽  
Vol 48 (1) ◽  
pp. 52-58 ◽  
Author(s):  
Ronaldo Zantedeschi ◽  
Anderson Dionei Grützmacher ◽  
Juliano de Bastos Pazini ◽  
Flávio Amaral Bueno ◽  
Larissa Longaray Machado
Keyword(s):  
Biological Control ◽  
Stink Bug ◽  
Trissolcus Basalis ◽  
Glufosinate Ammonium ◽  
Soybean Crop ◽  
Randomized Design ◽  
Lambda Cyhalothrin ◽  
Completely Randomized Design ◽  
Telenomus Podisi ◽  
Laboratory Bioassays

ABSTRACT The use of pesticides in the soybean crop may impair the biological control, if the products are not selective to Telenomus podisi and Trissolcus basalis, which are important stink bug egg parasitoids. This study aimed to assess the selectivity of 15 pesticides registered for soybean crop on adults of T. podisi and T. basalis. Three laboratory bioassays were performed, totaling 18 treatments, in a completely randomized design, with four replications. The insecticides (g or mL of a.i.) imidacloprid + beta-cyfluthrin (0.25 + 0.03), deltamethrin (0.06), thiamethoxam + lambda-cyhalothrin (0.27 + 0.35), acephate (1.87) and fenitrothion (1.25) are harmful to both parasitoids species. Flubendiamide (1.20), diflubenzuron (2.00), Bacillus thuringiensis (0.08) and lufenuron (0.12) are innocuous to both parasitoids. The fungicides azoxystrobin + flutriafol (0.31 + 0.31), trifloxystrobin + prothioconazole (0.37 + 0.44), pyraclostrobin + metconazole (0.33 + 0.12) and trifloxystrobin + cyproconazole (0.94 + 0.40) are innocuous to T. podisi, but slightly harmful to T. basalis. The herbicides glufosinate ammonium salt (0.50) and glyphosate isopropylamine salt (1.20) are innocuous to both parasitoids.

scholarly journals Evaluation of cellulose substrates treated with Metarhizium anisopliae (Metschnikoff) Sorokin as a biological control agent against the termite Microcerotermes diversus Silvestri (Isoptera: Termitidae)

2011 ◽  
Vol 43 (2) ◽  
pp. 269
Author(s):  
Behzad Habibpour ◽  
Amir Cheraghi ◽  
Mohammad Saeed Mossadegh
Keyword(s):  
Biological Control ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Biological Control Agent ◽  
Control Agent ◽  
Cellulose Substrates ◽  
Virulent Strains ◽  
Sustainable Protection ◽  
Chemical Pesticides

This article is the first report on the promising effect of an entomopathogenic fungus, <em>Metarhizium anisopliae</em> (Metschnikoff) Sorokin to control populations of <em>Microcerotermes diversus </em>Silvestri. Biological control is an alternative to the long-term usage of chemical pesticides.<em> M. anisopliae</em>, the causal agent of green muscardine disease of insects, is an important fungus in biological control of insect pests. Bait systems can eliminate entire colonies of subterranean termites. Baiting reduces adverse environmental impacts caused by organochlorine and organophosphate pesticides in the control of termites and creates sustainable protection of buildings against their invasion. Treated-sawdust bait was applied by two methods: a) combination of treated sawdust and untreated filter paper, and b) combination of treated sawdust and untreated sawdust. When combinations of treated sawdust and untreated sawdust were used, LC50 and LC90 were 8.4&times;106 and 3.9&times;107 (spore/ml), respectively. With the use of improved bait formula and more virulent strains, we hope to achieve better control of termite colonies and enable pathogens to become a useful element in the Integrated Pest Management system.

scholarly journals Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of South Sumatra, Indonesia

2018 ◽  
Vol 19 (6) ◽  
pp. 2365-2373 ◽  
Author(s):  
AYU SAFITRI ◽  
SITI HERLINDA ◽  
ARUM SETIAWAN
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Vegetation Type ◽  
Tenebrio Molitor ◽  
Soil Samples ◽  
Fungal Isolates

Safitri A, Herlinda S, Setiawan A. 2018. Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of South Sumatra, Indonesia. Biodiversitas 19: 2365-2373. Ecosystems of lowlands and highlands in South Sumatra have specific characteristics of soils and vegetations that can affect the availability of entomopathogenic fungi. This study aimed to explore and identify species and to determine inoculum potentials of the entomopathogenic fungi from soils of freshwater swamps, tidal lowlands, peatlands, and highlands. Baiting of entomopathogenic fungi on soil samples used the larvae of Tenebrio molitor. The entomopathogenic fungi species found in this research were Beauveria bassiana and Metarhizium anisopliae. The number of the fungal isolates were 30 isolates consisting of nine isolates of B. bassiana and 21 isolates of M. anisopliae.The highest number of isolates was found in the highland ecosystem (11 isolates) and the lowest was found in peatland ecosystem (4 isolates). The highest percentage average of inoculum potentials of the fungi was found in the high land ecosystem (4.04%) and the lowest one was found in freshwater swamps ecosystem (2.11%). Based on the vegetation type, the soil planted with mustard in Talang Patai-Pagaralam (highland ecosystem) had the highest inoculum potentials (9.33%). These fungi will make an important contribution to the biological control for insect pests in lowland to highland ecosystems in Indonesia.

scholarly journals Exploration and Effectiveness of Trichoderma sp. from Jember and Trenggalek, East Java, Indonesia Cacao Plantation as A Biological Control of Phytophthora palmivora

2021 ◽  
Vol 226 ◽  
pp. 00022
Author(s):  
Henik Sukorini ◽  
Feby Wirasdenty Aigahayunindy ◽  
Erfan Dani Septia ◽  
Netnapis Khewkhom
Keyword(s):  
Biological Control ◽  
Fruit Rot ◽  
Phytophthora Palmivora ◽  
Randomized Design ◽  
Trichoderma Sp ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Rot Disease ◽  
Cacao Plantation ◽  
All Treatment

Fruit rot disease is very damaging to cacao pods, which is caused by Phytoptora palmivora. The attack rate of P. palmivora varies. In Java, losses due to this disease reduce yields by 90 %. P. palmivora is a soil–borne pathogen. It is currently included in the Kingdom Chromista. Control with fungicides is not successful at this time, the alternatives is biologis control with Trichoderma sp. This research used a Completely Randomized Design (CRD) which was arranged in factorial with two factors. The first factor was Trichoderma sp. the second factor was P. palmivora. All treatment combinations were repeated three times. Trichoderma sp. antagonist test to P. palmivora was analyzed using Analysis of Variance (ANOVA) and then further tested using a 5 % BNJ. Trichoderma sp. origin from Jember and Trenggalek districts, East Java, Indonesia were able to act as antagonists against P. palmivora with the highest inhibitory of 78 %. In comparison, the lowest inhibitory was 70 % of isolates from Jember district, East java, Indoe. Characteristics of Trichoderma sp. The origin of Trenggalek Regency and Jember Regency, East Java, Indonesia in inhibiting the growth of P. palmivora has the same species, namely Trichoderma harzianum.

scholarly journals Effect of Laserpuncture Shoot on Reproduction Point of Male Mojosari Duck (Anas Platyrhynchos) on The Numbers of Spermatogonium Cells and Seminiferous Tubules Diameter

2017 ◽  
Vol 3 (6) ◽  
pp. 718
Author(s):  
Melyandari Ayu Qomar ◽  
Rimayanti Rimayanti ◽  
Tri Nurhajati
Keyword(s):  
Seminiferous Tubule ◽  
Anas Platyrhynchos ◽  
Research Result ◽  
Seminiferous Tubules ◽  
Group Iii ◽  
Randomized Design ◽  
Group I ◽  
Histological Slide ◽  
Completely Randomized Design ◽  
Tubule Diameter

The Objective of this research was to know the effect of laserpuncture on reproduction point of male Mojosari duck on the number of spermatogonium cells and seminiferous tubule diameter of the testes. This research using completely randomized design with eighteen male Mojosari ducks which divided into three group randomly. Group I (P0) without any treatment of laserpuncture, Group II (P1) with 0.2 J dose of laserpuncture, and Group III (P2) with 0.4 J dose of laserpuncture. Laserpuncture shooting was done on reproduction point with three days interval in one month. On the thirty-first day, the duck was dissected so that the testes can be taken then made by the histological slide of testes. The obtained data processed by Analysis of Variant (ANOVA) followed with Duncan. Research result showed that laserpuncture shoot on the reproduction point of five-month-old male Mojosari duck decreases the number of spermatogonium cells and seminiferous tubule diameter of the testes. Keyword: laserpuncture, Mojosari duck, spermatogonium, seminiferous tubule

scholarly journals Isolation and Characterization of Photorhabdus Spp. (Enterobacteriaceae) Isolated From Heterorhabditis Bacteriophora in Kashere, Nigeria

2021 ◽  
Vol 25 (8) ◽  
pp. 1345-1348
Author(s):  
H.U. Aliyu ◽  
F. Tahir ◽  
E.B. Agbo ◽  
S.L. Kela
Keyword(s):  
Biological Control ◽  
Bacterial Isolate ◽  
Insect Pests ◽  
Entomopathogenic Nematode ◽  
Heterorhabditis Bacteriophora ◽  
Biochemical Tests ◽  
Susceptible Host ◽  
Isolation And Characterization ◽  
Biological Control Potential

Photorhabdus bacteria are symbiotically associated with the infective juveniles of entomopathogenic nematodes of the genus Heterorhabditis. These bacteria after infecting a susceptible host, produces a host of lethal toxins that killthe insect hosts within 24 to 72 hours. Consequently they have emerged and are trending as excellent biological control agents against insect pests of agricultural crops. Therefore, this study aim to isolate, identify and test the pathogenicity of Photorhabdus bacteria isolated from Heterorhabditis bacteriophora, entomopathogenic nematode from Kashere, Gombe State, Nigeria. To this end phenotypic and biochemical tests were conducted. The tests conducted showed that the isolate exhibited characteristics similar to those of Photorhabdus bacteria that have been identified. Last instar larvae of G. mellonella exposed to different concentrations of the bacterial isolate showed some level of susceptibility of the larvae to the bacterial isolate confirming its biological control potential.

scholarly journals PENGARUH LEVEL BOKASHI KOTORAN AYAM TERHADAP PERTUMBUHAN VEGETATIF SORGUM BROWN MIDRIB (BMR) PADA KONDISI TERNAUNG

ZOOTEC ◽  
2017 ◽  
Vol 38 (1) ◽  
pp. 50
Author(s):  
Claudia F. Rawung ◽  
S.D Anis ◽  
Rustandi . ◽  
W.B. Kaunang
Keyword(s):  
Plant Height ◽  
Vegetative Growth ◽  
Research Result ◽  
Chicken Manure ◽  
Leaf Number ◽  
Brown Midrib ◽  
Leaf Analysis ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Better Than

THE INFLUENCE CHICKEN MANURE BOKASHI ON VEGETATIVE GROWTH OF BROWN MIDRIB SORGHUM (BMR) UNDER SHADE. The aim of this experiment was to study the effects of application of chicken manure bokashi on the vegetative growth of Brown Midrib (BMR) sorghum.  Completely Randomized Design (CRD) has been used according to Steel and Torrie (1995). The treatments were level of chicken manure  bokashi as follows B1= 0 gram; B2= 200 gram; B3= 400 gram and B4=  800 gram/polybag, with 7 replications to form 28 numbers of observations. The variables measured were plant height, leaf number and  length of leaf. Analysis of Variance showed that treatments effected significanly different (P<0.05) on plant height, leaf number and length of leaf. Result of Tuckey Simultenaus Test showed that between treatment B3 and B4 were not significant different (P>0.05) on all variable measured, but both treatments gave a good performance of all variable measured significantly (P<0,05) better than treatments  B2 and B1. Based on this research result it can be concluded that utilization of chicken manure bokashi up to 400 gram/polybag could provide nutrients requirement to support vegetative growth of sorghum BMR in term of height of plant, leaf number and length of leaf. Keywords: Bokashi, chicken manure, growth, BMR sorghum, shaded.

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