Cell lines, Md108 and Md66, from the hemocytes of Malacosoma disstria (Lepidoptera) display aspects of plasma-free innate non-self activities

2011 ◽  
Vol 108 (3) ◽  
pp. 180-193 ◽  
Author(s):  
Jason F. Lapointe ◽  
Gary B. Dunphy ◽  
Paschalis Giannoulis ◽  
Craig A. Mandato ◽  
James B. Nardi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Malacosoma Disstria

Isolation and characterization of plaque-purified strains of Malacosoma disstria Nucleopolyhedrovirus

2006 ◽  
Vol 52 (3) ◽  
pp. 266-271 ◽  
Author(s):  
Martin A Erlandson ◽  
Doug Baldwin ◽  
Melissa Haveroen ◽  
B Andrew Keddie
Keyword(s):  
Restriction Endonuclease ◽  
Cell Line ◽  
Cell Lines ◽  
Field Isolate ◽  
Malacosoma Disstria ◽  
Forest Tent Caterpillar ◽  
Isolation And Characterization ◽  
Budded Virus ◽  
Restriction Endonuclease Fragment ◽  
Tent Caterpillar

Seven plaque-purified genotypic variants or strains, derived from a previously described field isolate of the Malacosoma disstria Nucleopolyhedrovirus (MadiNPV) from Alberta populations of forest tent caterpillar, were characterized based on distinctive restriction endonuclease fragment patterns. Two strains, MadiNPV-pp3 and MadiNPV-pp11, were selected for further characterization, as they represented strains producing high and low budded virus (BV) titres, respectively, in the M. disstria cell line UA-Md203. Analysis of restriction endonuclease fragment profiles indicated the genomes differed significantly in size, 133.8 ± 2.4 kb for MadiNPV-pp3 and 118.1 ± 3.5 kb for MadiNPV-pp11. These strains were characterized based on their BV production in three different cell lines derived from M. disstria haemocytes. Compared with MadiNPV-pp11, MadiNPV-pp3 produced two- to three-fold more BVs in UA-Md203 and 210 other cell lines; however, BV production was only marginally higher for MadiNPV-pp3 in the UA-Md221 cell line. Similarly, the yield of polyhedral inclusion bodies was significantly higher for MadiNPV-pp3 in UA-Md203 and 210 cell lines than for MadiNPV-pp11 but not in the UA-Md221 cell line. This data, although derived from a limited number of cell lines, suggested MadiNPV-pp3 may have a broader tissue tropism than MadiNPV-pp11.Key words: forest tent caterpillar, Malacosoma disstria, Nucleopolyhedrovirus.

Persistent infection of Malacosoma disstria (Lepidoptera: Lasiocampidae) cell cultures with Nosema (Glugea) disstriae (Microsporida: Nosematidae)

1976 ◽  
Vol 54 (3) ◽  
pp. 336-342 ◽  
Author(s):  
S. S. Sohi ◽  
G. G. Wilson
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Persistent Infection ◽  
Malacosoma Disstria

Cell lines were developed from hemocytes and ovarian tissues of Malacosoma disstria larvae that were naturally infected with Nosema disstriae. The infection was carried over into the cell cultures. N. disstriae disappeared from the ovarian cultures after several passages in vitro involving a period of more than 2 years, but hemocyte cultures are still infected after 6 years (199 passages). The spores produced in cell cultures were infectious for M. disstria larvae.

CHROMOSOMAL CHARACTERISATION OF FIVE LEPIDOPTERAN CELL LINES OF MALACOSOMA DISSTRIA (LASIOCAMPIDAE) AND CHRISTONEURA FUMIFERANA (TORTRICIDAE)

1976 ◽  
Vol 18 (3) ◽  
pp. 471-477 ◽  
Author(s):  
T. J. Ennis ◽  
S. S. Sohi
Keyword(s):  
Chromosome Number ◽  
Cell Lines ◽  
Choristoneura Fumiferana ◽  
Malacosoma Disstria ◽  
Large Chromosome ◽  
Cell Nuclei ◽  
Sex Chromatin ◽  
Established Cell ◽  
High Level ◽  
Lepidopteran Cell Lines

Chromosome number and morphology have been examined in four established cell lines (Md63, Md66, Md108, and Md109) of the forest tent caterpillar, Malacosoma disstria Hübner, and one (Cf124) of the spruce budworm, Choristoneura fumiferana (Clemens). Chromosome number distributions of Md63 (mode = 112), Md108 (mode = 103), Md109 (mode = 103), and Cf124 (mode = 110) overlap sufficiently to prevent identification of individual lines by number alone. However, Md66 is exceptional in possessing a modal number of 157. One large chromosome occurs in cells of all lines. The presence of this chromosome, the lack of any distinct polyploid series among chromosome numbers encountered, and the general inverse relationship between number and size of chromosomes, suggest that the high level of heteroploidy characteristic of these and other lepidopteran cell lines reflects not only a possible polyploid origin but also extensive chromosomal rearrangement and fragmentation. Tolerance for such change is attributed to the holokinetic organisation of lepidopteran chromosomes. A distinct heteropycnotic body is present in about 10% of Cf124 cell nuclei, and can be used as a marker for this line. This body may represent the sex chromatin normally encountered in somatic cells of female C. fumiferana.

Serologic characterization and identification of four lepidopteran cell lines

1976 ◽  
Vol 54 (9) ◽  
pp. 1559-1564 ◽  
Author(s):  
J. Krywienczyk ◽  
S. S. Sohi
Keyword(s):  
Cell Lines ◽  
Tissue Specificity ◽  
Choristoneura Fumiferana ◽  
Double Diffusion ◽  
Malacosoma Disstria ◽  
Tissue Cultures ◽  
Species Identity ◽  
Strong Reaction ◽  
Lepidopteran Cell Lines

The identity of four cell lines (Cf124, Md63, Md108, and Md109) was investigated using the technique of double diffusion. The absorbed antiserum of Cf124 cells formed strong precipitin lines with Choristoneura fumiferana antigen, and the absorbed antisera of Md63, Md108, and Md109 cells gave a strong reaction with the Malacosoma disstria antigen, thus substantiating their species identity. Although the M. disstria cell lines did not show any tissue specificity, they differed from each other in at least one antigen. The antigens from whole larvae of C. fumiferana and M. disstria were more suitable than their hemolymph for serological reactions with the antisera of tissue cultures by double diffusion.

Establishment and Characterization of Three Malacosoma disstria Cell Lines

1995 ◽  
Vol 66 (2) ◽  
pp. 136-142 ◽  
Author(s):  
B.Andrew Keddie ◽  
Martin A. Erlandson ◽  
Gerald J. Hilchie
Keyword(s):  
Cell Lines ◽  
Malacosoma Disstria

Periodic Structure in the Matrix Protein of an Insect Virus

1982 ◽  
Vol 40 ◽  
pp. 302-303
Author(s):  
H.M. Mazzone ◽  
W.F. Engler ◽  
R. Zerillo ◽  
G.F. Bahr
Keyword(s):  
Inclusion Body ◽  
Periodic Structure ◽  
Matrix Protein ◽  
Malacosoma Disstria ◽  
Insect Virus ◽  
Virus Particles ◽  
The Matrix ◽  
Nucleopolyhedrosis Virus

The nucleopolyhedrosis virus (NPV) of the forest tent cater - pillar (Malacosoma disstria Hubner) has been analyzed in our laboratories. As a representative of the Baculovirus class, the NPV has virus particles enclosed with in a proteinaceous structure, the inclusion body.

(EB) Virus: Latency and Expression in Transplanted and Cultured Human Cells

1971 ◽  
Vol 29 ◽  
pp. 368-369
Author(s):  
B. G. Uzman ◽  
M. M. Kasac ◽  
H. Saito ◽  
A. Krishan
Keyword(s):  
Cell Lines ◽  
Primary Cultures ◽  
Virus Particles ◽  
Barr Virus ◽  
Virus Latency ◽  
Virus Surveillance ◽  
Cultured Human Cells ◽  
Epstein Barr ◽  
Serial Transplantation ◽  
Tubular Arrays

In conjunction with the cultivation and transplantation of cells from human tumors by the Programs of Microbiology and Immunogenetics, virus surveillance by electron microscopy has been routinely employed. Of particular interest in this regard have been 3 cell lines cultured from lymph nodes or spleen of 2 patients with Hodgkin's disease and 1 patient with Letterer-Siwe's disease. Each of these cell lines when transplanted in Syrian hamster neonates conditioned with anti-lymphocyte serum grew as serially transplantable tumors; from such transplants of the 3 cell lines cell cultures were retrieved.Herpes type virus particles (Figs. 1, 2, 3) were found in the primary cultures of all three lines, in frozen thawed aliquots of same, and in cultures retrieved from their tumors growing by serial transplantation in hamsters. No virus was detected in sections of 25 of the serially transplanted tumors. However, in 10 such tumors there were repeated instances of tubular arrays in the cisternae of the endoplasmic reticulum (Fig. 4). On serologic examination the herpes virus was shown to be the Epstein-Barr virus.

Comparison of Choriocarcinoma and Normal Placenta

1971 ◽  
Vol 29 ◽  
pp. 324-325
Author(s):  
John C. Garancis ◽  
Roland A. Pattillo ◽  
Robert O. Hussa ◽  
Jon V. Straumfjord
Keyword(s):  
Cell Lines ◽  
Small Cells ◽  
Tissue Cultures ◽  
Glycogen Depletion ◽  
Cell Surfaces ◽  
Intercellular Spaces ◽  
Concomitant Increase ◽  
Gestational Choriocarcinoma ◽  
Cytoplasmic Glycogen ◽  
Normal Placenta

Two different cell lines (Be-Wo and Jar) of human gestational choriocarcinoma have been maintained in continuous tissue culture for a period of four and two years respectively without losing the ability to elaborate human chorionic gonadotropin (HCG). Tissue cultures, as revealed by electron microscopy, consisted of small cells with single nuclei. In some instances cell surfaces were provided with microvilli but more often the intercellular spaces were narrow and bridged by desmosomes. However, syncytium was not formed. Endoplasmic reticulum (ER) was poorly developed in both cell lines, except in some Be-Wo cells it was prominent. Golgi complex, lysosomes and numerous free ribosomes, as well as excessive cytoplasmic glycogen, were present in all cells (Fig. 1). Glycogen depletion and concomitant increase of ER were observed in many cells following a single dose of 10 ugm/ml of adrenalin added to medium (Fig. 2).

Immunocytochemical localization of β1-4 galactosyltransferase in endometrial carcinoma cells

1990 ◽  
Vol 48 (3) ◽  
pp. 944-945
Author(s):  
Ichiro Yamamoto ◽  
Toshiaki Tachibana ◽  
Hiroko Maruyama ◽  
Noriyuki Komatsu ◽  
Hiroyuki Kuramoto ◽  
...  
Keyword(s):  
Endometrial Carcinoma ◽  
Cell Lines ◽  
Endometrial Adenocarcinoma ◽  
Protein A ◽  
Rabbit Antiserum ◽  
Carcinoma Cells ◽  
Affinity Column ◽  
Antibody Technique ◽  
Galactosyl Transferase ◽  
C Terminus

We have paid attention to the alteration of glycosyltransferase in carcinoma cells, because it might be related to the malignancy of the cells. In this connection, localization of β1-4 galactosyl transferase (β1-4 Gal T) in human endometrial carcinoma cells was examined immunocytochemically using two kinds of cell lines, each of which showed different degree of differentiation.An antibody was purified from the rabbit antiserum against the synthetic peptide, IFNRLVFRGMSC (W89) of human β1-4 Gal T coupled with KLH (keyhole limpet hemocyanine) by protein A column and peptide-affinity column chromatography. The anti-W89 serum reacts to the C-terminus of human β 1-4 Gal T and to both membrane-bound and soluble forms of the enzyme. Cell line of well differentiated endometrial adenocarcinoma (I) and that of poorly differentiated endometrial adenocarcinoma (50B) were cultivated respectively in MEM medium containing 15% FCS and 2 mM glutamine for 4 d at 37°C under 5% CO2. The cells were fixed in a mixture of 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1 M Soerensen’s phosphate buffer (pH 7.4) at 4°C for 30 min, washed with PBS, then freezed and thawed. The indirect method of the peroxidase- labeled antibody technique was used for immunocytochemistry of both LM and TEM on the cell lines. The cells were dehydrated in ethanol and embedded in TAAB 812. Ultrathin sections were observed under a TEM, JEM-100S.

Absence of temporal ordering of apoptotic features in heat-shock treated leukemia and lymphoma cell lines

1996 ◽  
Vol 54 ◽  
pp. 758-759
Author(s):  
D. W. Fairbain ◽  
M.D. Standing ◽  
K.L. O'Neill
Keyword(s):  
Heat Shock ◽  
Cell Lines ◽  
Chromatin Condensation ◽  
Membrane Integrity ◽  
Resistant Cell ◽  
Membrane Blebbing ◽  
Late Loss ◽  
Induced Apoptosis ◽  
Dying Cells ◽  
In Cells

Apoptosis is a genetically defined response to physiological stimuli that results in cellular suicide. Features common to apoptotic cells include chromatin condensation, oligonucleosomal DNA fragmentation, membrane blebbing, nuclear destruction, and late loss of ability to exclude vital dyes. These characteristics contrast markedly from pathological necrosis, in which membrane integrity loss is demonstrated early, and other features of apoptosis, which allow a non-inflammatory removal of dead and dying cells, are absent. Using heat shock-induced apoptosis as a model for examining stress response in cells, we undertook to categorize a variety of human leukemias and lymphomas with regard to their response to heat shock. We were also interested in determining whether a common temporal order was followed in cells dying by apoptosis. In addition, based on our previous results, we investigated whether increasing heat load resulted in increased apoptosis, with particular interest in relatively resistant cell lines, or whether the mode of death changed from apoptosis to necrosis.

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