Identification of Parkinson's disease biomarkers through RNA sequencing of peripheral blood leukocytes

2021 ◽  
Vol 429 ◽  
pp. 119469
Author(s):  
Valentina Tommasini ◽  
Mauro Catalan ◽  
Maurizio Romano ◽  
Giulia Mazzon ◽  
Tatiana Cattaruzza ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Rna Sequencing ◽  
Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Disease Biomarkers

Reduced Content of α-Synuclein in Peripheral Blood Leukocytes of Patients with LRRK2-Associated Parkinson’s Disease

2011 ◽  
Vol 150 (6) ◽  
pp. 679-681 ◽  
Author(s):  
S. N. Pchelina ◽  
A. K. Emelyanov ◽  
A. F. Yakimovskii ◽  
D. W. Miller ◽  
I. G. Shabalina ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes

scholarly journals RNA Sequencing Reveals LINC00167 as a Potential Diagnosis Biomarker for Primary Osteoarthritis: A Multi-Stage Study

2021 ◽  
Vol 11 ◽  
Author(s):  
Liying Jiang ◽  
Yiqin Zhou ◽  
Junjie Shen ◽  
Yi Chen ◽  
Ziyuan Ma ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Rna Sequencing ◽  
Peripheral Blood ◽  
Integrated Approach ◽  
Pcr Analysis ◽  
Healthy Controls ◽  
Peripheral Blood Leukocytes ◽  
Characteristic Analysis ◽  
Blood Leukocytes ◽  
Critical Pathways

ObjectivesGiven the roles played by lncRNA in human diseases and the high incidence of OA, this study investigated the pivotal pathways involved in the disease and identified potential biomarkers for OA diagnosis.MethodsWe first performed an exploration of RNA-sequencing in peripheral blood leukocytes from six subjects (3 OA and 3 healthy controls). Promising candidate lncRNAs were evaluated in first stage validation using a GEO dataset (GSE114007) of 38 subjects (20 OA and 18 healthy controls), followed by a second stage validation using quantitative PCR analysis with 101 subjects (67 OA and 34 controls). The third stage investigated the potential value of validated lncRNA in the early diagnosis of OA in peripheral blood leukocytes from a total of 120 participants (60 cases and 60 controls).ResultsThe dataset identified a total of 1,380 up-regulated and 719 down-regulated mRNAs and 5,743 up-regulated and 7,384 down-regulated lncRNAs. The up-regulated DEGs were mainly enriched in the extracellular matrix, while the down-regulated DEGs were mainly enriched in the IL-17 and wnt signaling pathways. 18 overlapping candidate lncRNAs survived after first-stage validation. 3 hub lncRNAs were selected for the second validation stage and qualified in an external sample, and lncRNA LINC00167 was further confirmed with a similar result (down-expressed in both stages). Receiver operating characteristic analysis showed that LINC00167 can distinguish OA cases from healthy controls with a high area under the curve of 0.879 (95%CI: 0.819, 0.938; P < 0.001), with a sensitivity of 80.7% and specificity of 83.5%.ConclusionThe expression profile of OA was identified and critical pathways were elucidated by an integrated approach to RNA-seq from easily accessible blood. LINC00167 may serve as a potential early diagnosis marker for OA in clinical practice. The detailed mechanism of action of this lncRNA requires further elucidation in future studies.

FUNCTIONING OF NO-SYNTHASE IN THE PERIPHERAL BLOOD LEUKOCYTES IN PATIENTS WITH ACNE VULGARIS

2018 ◽  
Vol 14 (66) ◽  
pp. 075
Author(s):  
H. S. Lavryk ◽  
O. P. Korniychuk ◽  
Z. Ya. Fedorovych ◽  
Z. D. Vorobets
Keyword(s):  
Peripheral Blood ◽  
Acne Vulgaris ◽  
No Synthase ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes
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