4-Hydroxynonenal, a lipid peroxidation product of dietary polyunsaturated fatty acids, has anticarcinogenic properties in colon carcinoma cell lines through the inhibition of telomerase activity

In colorectal cancer, CD133+ cells from fresh biopsies proved to be more tumorigenic than their CD133– counterparts. Nevertheless, the function of CD133 protein in tumorigenic cells seems only marginal. Moreover, CD133 expression alone is insufficient to isolate true cancer stem cells, since only 1 out of 262 CD133+ cells actually displays stem-cell capacity. Thus, new markers for colorectal cancer stem cells are needed. Here, we show the extensive characterization of CD133+ cells in 5 different colon carcinoma continuous cell lines (HT29, HCT116, Caco2, GEO and LS174T), each representing a different maturation level of colorectal cancer cells. Markers associated with stemness, tumorigenesis and metastatic potential were selected. We identified 6 molecules consistently present on CD133+ cells: CD9, CD29, CD49b, CD59, CD151, and CD326. By contrast, CD24, CD26, CD54, CD66c, CD81, CD90, CD99, CD112, CD164, CD166, and CD200 showed a discontinuous behavior, which led us to identify cell type-specific surface antigen mosaics. Finally, some antigens, e.g. CD227, indicated the possibility of classifying the CD133+ cells into 2 subsets likely exhibiting specific features. This study reports, for the first time, an extended characterization of the CD133+ cells in colon carcinoma cell lines and provides a “dictionary” of antigens to be used in colorectal cancer research.

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Effect of polyunsaturated fatty acids and antioxidants on lipid peroxidation in tissue cultures.

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)37347-8 ◽

1981 ◽

Vol 22 (5) ◽

pp. 763-769

Author(s):

V C Gavino ◽

J S Miller ◽

S O Ikharebha ◽

G E Milo ◽

D G Cornwell

Keyword(s):

Lipid Peroxidation ◽

Fatty Acids ◽

Polyunsaturated Fatty Acids ◽

Tissue Cultures

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Various dietary polyunsaturated fatty acids modulate acrylamide-induced preneoplatic urothelial proliferation and apoptosis in mice

Experimental and Toxicologic Pathology ◽

10.1016/j.etp.2008.12.007 ◽

2010 ◽

Vol 62 (1) ◽

pp. 9-16 ◽

Cited By ~ 5

Author(s):

Xichun Zhang ◽

Congcong Zhao ◽

Baoshi Jie

Keyword(s):

Fatty Acids ◽

Polyunsaturated Fatty Acids ◽

Proliferation And Apoptosis ◽

Dietary Polyunsaturated Fatty Acids

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INDUCTION OF APOPTOSIS IN HL-60 CELLS BY DIETARY POLYUNSATURATED FATTY ACIDS.

Shock ◽

10.1097/00024382-200106001-00219 ◽

2001 ◽

Vol 15 (Supplement) ◽

pp. 73-74

Author(s):

R. Gillis ◽

P. Tithof ◽

N. Neilsen ◽

M. Barnhill ◽

R. Andrews ◽

...

Keyword(s):

Fatty Acids ◽

Polyunsaturated Fatty Acids ◽

Dietary Polyunsaturated Fatty Acids ◽

Induction Of Apoptosis

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Quantification of Volatile Aldehydes Deriving from In Vitro Lipid Peroxidation in the Breath of Ventilated Patients

Molecules ◽

10.3390/molecules26113089 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3089

Author(s):

Lukas M. Müller-Wirtz ◽

Daniel Kiefer ◽

Sven Ruffing ◽

Timo Brausch ◽

Tobias Hüppe ◽

...

Keyword(s):

Lipid Peroxidation ◽

Fatty Acids ◽

Polyunsaturated Fatty Acids ◽

Breathing Circuit ◽

Aliphatic Aldehydes ◽

Mechanically Ventilated ◽

Non Invasive ◽

Plastic Parts ◽

Ventilated Patients

Exhaled aliphatic aldehydes were proposed as non-invasive biomarkers to detect increased lipid peroxidation in various diseases. As a prelude to clinical application of the multicapillary column–ion mobility spectrometry for the evaluation of aldehyde exhalation, we, therefore: (1) identified the most abundant volatile aliphatic aldehydes originating from in vitro oxidation of various polyunsaturated fatty acids; (2) evaluated emittance of aldehydes from plastic parts of the breathing circuit; (3) conducted a pilot study for in vivo quantification of exhaled aldehydes in mechanically ventilated patients. Pentanal, hexanal, heptanal, and nonanal were quantifiable in the headspace of oxidizing polyunsaturated fatty acids, with pentanal and hexanal predominating. Plastic parts of the breathing circuit emitted hexanal, octanal, nonanal, and decanal, whereby nonanal and decanal were ubiquitous and pentanal or heptanal not being detected. Only pentanal was quantifiable in breath of mechanically ventilated surgical patients with a mean exhaled concentration of 13 ± 5 ppb. An explorative analysis suggested that pentanal exhalation is associated with mechanical power—a measure for the invasiveness of mechanical ventilation. In conclusion, exhaled pentanal is a promising non-invasive biomarker for lipid peroxidation inducing pathologies, and should be evaluated in future clinical studies, particularly for detection of lung injury.

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Maternal dietary polyunsaturated fatty acids in the early development of the immune system

European Journal of Pharmacology ◽

10.1016/j.ejphar.2011.09.288 ◽

2011 ◽

Vol 668 ◽

pp. e39-e40

Author(s):

A. Hogenkamp⁎ ◽

N. van Vlies ◽

E. van Esch ◽

A. Fear ◽

P. Calder ◽

...

Keyword(s):

Fatty Acids ◽

Immune System ◽

Polyunsaturated Fatty Acids ◽

Early Development ◽

Dietary Polyunsaturated Fatty Acids

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Corrigendum to “Design and synthesis of functionalized glycomers as non-peptidic ligands for SH2 binding and as inhibitors of A-431 human epidermoid and HT-29 colon carcinoma cell lines” [Bioorg. Med. Chem. Lett. 10 (2000) 439]†

Bioorganic & Medicinal Chemistry Letters ◽

10.1016/s0960-894x(00)00554-0 ◽

2000 ◽

Vol 10 (23) ◽

pp. 2691

Author(s):

Stephen Hanessian ◽

Oscar M Saavedra ◽

Fang Xie ◽

Nadia Amboldi ◽

Carlo Battistini

Keyword(s):

Colon Carcinoma ◽

Cell Lines ◽

Carcinoma Cell ◽

Design And Synthesis ◽

Carcinoma Cell Lines ◽

Ht 29

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Carotid intima-media thickness and oxidative stress markers for assessment of atherosclerosis in children with β thalassemia major

Thalassemia Reports ◽

10.4081/thal.2016.4939 ◽

2016 ◽

Vol 6 (1) ◽

Author(s):

Geetanjali Jindal ◽

Prashant Chavan ◽

Ravinder Kaur ◽

Shivani Jaswal ◽

Kamal Kumar Singhal ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Oxidized Ldl ◽

Thalassemia Major ◽

Lipid Peroxidation Product ◽

Oxidative Stress Markers ◽

Lipoprotein A ◽

Stress Markers ◽

Total Antioxidant ◽

Peroxidation Product

The present study evaluates carotid intimamedia thickness (CIMT) in children with β thalassemia major to assess atherosclerosis and its relation to the underlying proposed causative mechanisms via lipid peroxidation product malondialdehyde (MDA), oxidized lowdensity lipoproteins (LDL), total antioxidant level, and lipid profile. A cross sectional study was conducted on 62 children (31 cases and 31 controls). CIMT by high resolution ultrasound and biochemical parameters i.e., total cholesterol, triglycerides, high-density lipoproteins, LDL, Oxidized LDL, lipoprotein (a), lipid peroxidation product MDA and total antioxidant were measured in enrolled subjects and compared. In our study, CIMT was significantly increased in β thalassemia major patients’ as compared to healthy controls. Mean CIMT in cases was 0.69±0.11 mm and in controls 0.51±0.07 mm. Mean oxidized LDL (EU/mL) in cases 39.3±34.4 (range 14.4 to 160) was significantly raised (P=0.02, t test) as compared to controls 23.9±13.4 (range 12 to 70). In our study we found MDA levels (nmol/mL) to be increased in β thalassemia patients as compared to controls. Mean MDA was 10.0±3.27 (4.41 to 17.48) in cases while in controls was 6.87±4.55 (1.5 to 17.9). Our study results show CIMT as an early marker of atherogenesis in β thalassemia major. Oxidative stress markers are also increased in β thalassemia major patients and lipoprotein (a) shows a positive correlation with CIMT. The present study points towards various atherogenetic mechanisms in β thalassemia major. 本研究评价β重型地中海贫血患儿颈动脉内膜中层厚度（CIMT），以评估动脉粥样硬化，以及与潜在通过血脂过氧化反应产物丙二醛（MDA）、氧化低密度脂蛋白（LDL）、总抗氧化水平和血脂谱所提出致病机制之间的关系。在62名儿童（31例病例和31例对照）中进行了一项横断面研究。在入组受试者中通过高分辨率超声和生化指标（即总胆固醇、甘油三酯、高密度脂蛋白、LDL、氧化LDL，脂蛋白（a）、血脂过氧化产物MDA和总抗氧化剂）测量CIMT并进行比较。在我们的研究中，CIMT在β重型地中海贫血患者中比健康对照组显著增加。病例组中的平均CIMT为0.69±0.11 mm，对照组0.51±0.07 mm。病例组中平均氧化LDL（EU/mL）为39.3±34.4（从14.4到160的范围）与对照组的23.9±13.4（12至70的范围）相比显著升高（P = 0.02，t检验）。在我们的研究中，我们发现β地中海贫血患者中的MDA水平（nmol/mL）比对照组更高。病例组中的平均MDA为10.0±3.27（4.41至17.48），而对照组为6.87±4.55（1.5到17.9）。我们的研究结果表明，CIMT是β重型地中海贫血动脉粥样硬化的早期标记物。氧化应激标记物在β重型地中海贫血患者中也有增加，脂蛋白（a）显示出与CIMT呈正相关。本研究针对β重型地中海贫血中的各种动脉粥样硬化机制。

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