An improved size exclusion-HPLC method for molecular size distribution analysis of immunoglobulin G using sodium perchlorate in the eluent

2017 ◽  
Vol 138 ◽  
pp. 330-343 ◽  
Author(s):  
Hsiaoling Wang ◽  
Mark S. Levi ◽  
Alfred V. Del Grosso ◽  
William M. McCormick ◽  
Lokesh Bhattacharyya
Keyword(s):  
Size Distribution ◽  
Immunoglobulin G ◽  
Sodium Perchlorate ◽  
Molecular Size ◽  
Hplc Method ◽  
Size Exclusion ◽  
Distribution Analysis ◽  
Size Exclusion Hplc ◽  
Molecular Size Distribution

Pretreatment of concentrated leachate by the combination of coagulation and catalytic ozonation with Ce/AC catalyst

2015 ◽  
Vol 73 (3) ◽  
pp. 511-519 ◽  
Author(s):  
Hangdao Qin ◽  
Honglin Chen
Keyword(s):  
Size Distribution ◽  
Membrane Separation ◽  
Oxygen Demand ◽  
Molecular Size ◽  
Apparent Molecular Weight ◽  
Catalytic Ozonation ◽  
Cod Removal ◽  
Distribution Analysis ◽  
Molecular Size Distribution ◽  
Ozonation Process

A raw concentrated leachate produced from membrane bioreactor-nanofiltration (MBR-NF) was taken from Chengdu Chang'an Waste Landfill Site, China. The major fraction of this concentrated leachate was large refractory humic substances. A coagulation–ozonation process was applied to treat this leachate, aiming at enhancing chemical oxygen demand (COD) removal efficiency and increasing its biodegradability. Meanwhile the molecular size distribution of the leachate, before and after coagulation and ozonation treatment, was analyzed by using ultrafiltration membrane separation. Coagulation pretreatment effectively removed varieties of large molecules in the raw concentrated leachate. The addition of Ce/AC greatly improved the oxidative ability of O3 in COD removal in the ozonation of coagulated leachate. The biochemical oxygen demand (BOD5)/COD ratio increased from 0.011 for the untreated concentrated leachate to 0.30 for the effluent of the coagulation–catalytic ozonation process, which indicated that a subsequent biological treatment could be readily conducted. The stability test demonstrated that the Ce/AC catalyst was effective and stable in the catalytic ozonation process. According to the results of molecular size distribution analysis, a direct correlation was observed between the increase of BOD5/COD and the decrease of apparent molecular weight.

scholarly journals Accumulation and changes in molecular size distribution of polymeric proteins in developing grains of hexaploid wheats: role of the desiccation phase

1999 ◽  
Vol 26 (4) ◽  
pp. 301 ◽  
Author(s):  
J. L. Carceller ◽  
T. Aussenac
Keyword(s):  
Molecular Weight ◽  
Size Distribution ◽  
High Molecular Weight ◽  
Molecular Size ◽  
Reversed Phase ◽  
Size Exclusion ◽  
Glutenin Subunits ◽  
Soluble Polymers ◽  
Molecular Size Distribution ◽  
Insoluble Polymers

Two varieties of wheat differing in high molecular weight glutenin subunit composition (Soissons, 5+10, Glu-D1a allele; Thésée, 2+12, Glu-D1a allele) were examined to follow the accumulation of polymeric proteins and the changes in molecular size distribution of these proteins during grain filling. The accumulation behaviour of polymeric proteins was determined by size-exclusion-HPLC, multistacking SDS-PAGE and the constituent polypeptides (high molecular weight and low molecular weight glutenin subunits) by reversed-phase-HPLC. For both cultivars, the accumulation of each class of protein was highly asynchronous, especially between the early deposition of SDS-soluble polymers and the late deposition of SDS-insoluble polymers, such that the average molecular size of polymeric protein increased in the period from 30 to 45 days after anthesis in natural conditions. By applying premature grain desiccations during the cell enlargement phase, it was demonstrated that the SDS-insoluble polymers formation was closely related with the process of water loss from the grain. Moreover, the rapid accumulation of SDS-insoluble polymers coincided with a rapid decrease in mass of both SDS-soluble polymers and monomers, suggesting an aggregative mechanism. Over the same period, the molecular size distribution of the polymers which can be used to differentiate the two genotypes studied, is highly correlated with the percentage of high molecular weight glutenin subunits in glutenins present in kernels when desiccation occurred. The formation of SDS-insoluble fraction is discussed in connection with the specific contribution of high molecular weight glutenin subunits to the formation of polymers (subunits linked by disulfide bonds).

scholarly journals A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Imran Khan ◽  
K. M. Taufiqur Rahman ◽  
S. M. Saad Us Siraj ◽  
Mahbubul Karim ◽  
Abdul Muktadir ◽  
...  
Keyword(s):  
Distribution Coefficient ◽  
Size Distribution ◽  
Size Exclusion Chromatography ◽  
Molecular Size ◽  
Polysaccharide Vaccine ◽  
Size Exclusion ◽  
Chromatography Method ◽  
Exclusion Chromatography ◽  
Calculated Distribution ◽  
Molecular Size Distribution

Molecular size distribution of meningococcal polysaccharide vaccine is a readily identifiable parameter that directly correlates with the immunogenicity. In this paper, we report a size exclusion chromatography method to determine the molecular size distribution and distribution coefficient value of meningococcal polysaccharide serogroups A, C, W, and Y in meningococcal polysaccharide (ACWY) vaccines. The analyses were performed on a XK16/70 column packed with sepharose CL-4B with six different batches of Ingovax® ACWY, a meningococcal polysaccharide vaccine produced by Incepta Vaccine Ltd., Bangladesh. A quantitative rocket immunoelectrophoresis assay was employed to determine the polysaccharide contents of each serogroup. The calculated distribution coefficient values of serogroups A, C, W, and Y were found to be0.26±0.16,0.21±0.11,0.21±0.11, and0.14±0.12, respectively, and met the requirements of British Pharmacopeia. The method was proved to be robust for determining the distribution coefficient values which is an obligatory requirement for vaccine lot release.

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