scholarly journals Impaired myeloid-derived suppressor cells are associated with recurrent implantation failure: A case-control study

2021 ◽  
Vol 145 ◽  
pp. 103316
Author(s):  
Huanhuan Jiang ◽  
Mengting Zhu ◽  
Peipei Guo ◽  
Kaihuan Bi ◽  
Zhimin Lu ◽  
...  
Keyword(s):  
Case Control Study ◽  
Suppressor Cells ◽  
Case Control ◽  
Implantation Failure ◽  
Myeloid Derived Suppressor Cells ◽  
Recurrent Implantation Failure ◽  
Control Study

scholarly journals Impaired Myeloid-derived Suppressor Cells Are Associated With Recurrent Implantation Failure: A Case-Control Study

2020 ◽  
Author(s):  
Huanhuan Jiang ◽  
Mengting Zhu ◽  
Peipei Guo ◽  
Kaihuan Bi ◽  
Zhimin Lu ◽  
...  
Keyword(s):  
T Cells ◽  
Case Control Study ◽  
Transforming Growth Factor ◽  
Suppressor Cells ◽  
Case Control ◽  
No Production ◽  
Implantation Failure ◽  
Myeloid Derived Suppressor Cells ◽  
Recurrent Implantation Failure ◽  
Control Study

Abstract Background: Studies have reported that myeloid-derived suppressor cells (MDSCs) contribute to maintain pregnancy. The aim of this case-control study was to test whether there is a dysregulation of peripheral MDSCs in recurrent implantation failure (RIF).Methods: 26 RIF patients and 30 controls were recruited. Flow cytometry was applied to characterize polymorphonuclear (PMN)-MDSCs, monocytic-MDSCs (M-MDSCs), effector T cells (Teffs) and regulatory T cells (Tregs) in blood. ELISA was used to define MDSCs correlative cytokines and chemokines in serum from all patients.Results: Compared with controls, RIF patients showed significant reductions of blood PMN-MDSCs, M-MDSCs, Tregs and NO production by PMN-MDSCs, whereas the expression of ζ chain on CD4+T cell receptor (TCR) and CD8+TCR displayed a remarkable upregulation in RIF patients. Moreover, RIF patients presented a lower concentration of serum chemokine (C-C motif) ligand (CCL) 5 and transforming growth factor (TGF)-β than those from controls. Furthermore, the level of TCR ζ chain on CD4+ and CD8+ Teffs was negatively correlated not only with the percentage of PMN-MDSCs, but also with the amount of NO produced by PMN-MDSCs. The frequency of PMN-MDSCs had positive correlations with the concentration of CCL5 and TGF-β.Conclusions: This study indicated that the dysregulation of MDSCs might impair maternal-fetal immune balance thus resulting in RIF.

scholarly journals Factor V H1299r (Hr2) Heterozygosity: A Risk Factor For Recurrent Implantation Failure Particularly In Non-Carriers For Factor V Leiden Mutation-A Case-Control Study

2021 ◽  
pp. 1-5
Author(s):  
A. Seval Ozgu-Erdinc ◽  
Pınar Gulsen Coban ◽  
Nafiye Yilmaz ◽  
Zuhal Candemir ◽  
Saynur Yilmaz ◽  
...  
Keyword(s):  
Case Control Study ◽  
Gene Mutations ◽  
Case Control ◽  
Factor V ◽  
Implantation Failure ◽  
Recurrent Implantation Failure ◽  
Factor V Leiden Mutation ◽  
History Of ◽  
Fv Leiden ◽  
Control Study

Objective: The association between recurrent implantation failure and thrombophilia is still controversial depending on the published reports with conflicting results. In this study, we aimed to assess the clinical relevance of screening women with recurrent implantation failure for some thrombophilic variants including factor V H1299R (FV HR2) haplotype. Study Design: A total of 279 women were recruited in this case-control study. 229 women with a history of recurrent implantation failure and 50 fertile control with no history of pregnancy losses were screened for eight specific gene mutations, regarding factor V G1691A gene (FV Leiden), FV HR2, factor II prothrombin G20210A, factor XIII V34L, PAI-1 4G/5G, MTHFR C677T, MTHFR A1298C and A3 haplotype of the endothelial cell protein C receptor gene. Results: Recurrent implantation failure group displayed a significantly higher prevalence of FV HR2 heterozygosity than fertile controls while the frequency of FV Leiden mutation was comparable between groups (p=0.011; p=0.619). Additionally, the difference in the prevalence of other specific or total gene mutations among women with recurrent implantation failure was also insignificant. Discussion: The primer outcome of this study was the co-existence of the higher prevalence of FV HR2 haplotype and the insignificant percentage of FV Leiden mutation in women with recurrent implantation failure. Thus, we emphasize that the HR2 haplotype may be associated with recurrent implantation failure particularly in non-carriers for FV Leiden mutation. In the necessity of screening for thrombophilia in recurrent implantation failure, HR2 haplotype should be involved in the searched gene panel particularly in the absence of FV Leiden mutation. Further large-scale prospective studies are needed to investigate whether screening or treatment for HR2 haplotype has any detrimental impact on implantation success in cases of recurrent implantation failure.

scholarly journals Analysis of 14-bp HLA-G polymorphism in middle-eastern women with recurrent implantation failure

2020 ◽  
Vol 9 (12) ◽  
pp. 4820
Author(s):  
Rand Sulaiman ◽  
Thara Mohamad ◽  
Marwan Alhalabi
Keyword(s):  
Case Control Study ◽  
Middle Eastern ◽  
Human Leukocyte ◽  
Case Control ◽  
Control Group ◽  
Polymorphism Analysis ◽  
Implantation Failure ◽  
Recurrent Implantation Failure ◽  
Significant Difference ◽  
Control Study

Background: Implantation appears to be the major rate-limiting step in assisted reproductive techniques success. Recurrent implantation failure (RIF) is a common condition, being the failure to achieve a clinical pregnancy after transfer of four or more good-quality embryos in a minimum of two IVF cycles in a woman under the age of 40. Human leukocyte antigen G (HLA-G) is thought to play a key role in implantation by modulating cytokine secretion to enable the semi-allogeneic embryo to implant into the maternal endometrium. The aim of this study was to investigate the association between the 14-bp ins/del HLA-G polymorphism and rates of RIF. No similar studies were conducted nationally or regionally.Methods: The study was designed as a case-control study. A proportion of 30 patients with RIF were selected as the experimental group. The control group included 15 patients with at least one pregnancy following IVF procedure. Peripheral blood samples were collected from both groups. DNA was extracted and the polymorphism analysis was performed using (AMPLI - HLA-G polymorphism) kit, then PCR products were electrophoresed on 2% agarose gel. statistical analysis was performed using SPSS statistics software.Results: A total of 45 middle-eastern patients were recruited in this case-control study. High frequency of the polymorphism was found in both case (76.6%) and control (86.67%) groups, but with no significant difference between the two groups (p=0.429).Conclusions: There is no correlation between the 14-bp ins/del HLA-G polymorphism and RIF, thus it may be considered as a normal polymorphism and might not affect the level of HLA-G.

scholarly journals The relationship between recurrent implantation failure and sexual function in infertile Iranian women: A case-control study

2021 ◽  
Author(s):  
Samira Ghorbani ◽  
Khadijeh Hekmat ◽  
Parvin Abedi ◽  
Saeed Ghanbari ◽  
Narjes Dibavand
Keyword(s):  
Sexual Function ◽  
Case Control Study ◽  
Case Control ◽  
Implantation Failure ◽  
Iranian Women ◽  
Recurrent Implantation Failure ◽  
Chi Square ◽  
Infertile Women ◽  
The Relationship ◽  
Control Study

Abstract Background Recurrent implantation failure affects 3–5% of women, which means failing to implant after two or more high-quality embryo transfer cycles. The aim of this study was to assess the relationship between recurrent implantation failure and sexual function in infertile Iranian women. Methods This was a case-control study of 180 infertile Iranian women (90 infertile women with recurrent implantation failure and 90 infertile women with no implantation failure). A demographic questionnaire and the Female Sexual Function Index were used for data collection. Data analyzed by means of chi-square, independent t-test and linear regression. Results The mean scores of different domains of sexual function including (desire, lubrication, arousal, orgasm, pain, satisfaction) were significantly lower in the group with RIF compared to the group without RIF. The total score of sexual function was significantly lower in the RIF group than in the RIF-free group (23.11 ± 2.24, vs. 25.99 ± 2.35, p < 0.001). Women in the RIF group were 2.65 times more likely to have a low score of sexual function compared to the women with no RIF (p < 0.001). Conclusion Women experiencing recurrent implantation failure may be at particular risk for reduced sexual function. Sexual function issues should be treated as an important component of comprehensive care.

scholarly journals Consistent Role of Insulin Resistance in Recurrent Pregnancy Loss and Recurrent Implantation Failure: A Case-control Study

2021 ◽  
Author(s):  
Miao Ding ◽  
Fengyi He ◽  
Xiaojia Li ◽  
Sushi Jiang ◽  
Yacong Cao ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Case Control Study ◽  
Case Control ◽  
Model Assessment ◽  
Implantation Failure ◽  
Recurrent Implantation Failure ◽  
Control Study

Abstract Background: To explore the role of insulin resistance (IR) in patients with recurrent pregnancy loss (RPL) and/or recurrent implantation failure (RIF) treated with assisted reproductive technology (ART).Methods: We conducted a case-control study in a tertiary hospital from 2012 to 2018, We included 212 cases of simple RPL (only involved in RPL), 123 cases of simple RIF (only involved in RIF), 67 cases involved in both conditions (complicated group). We screened 123 women as the control cohort, who underwent ART due to male infertile, with no adverse pregnant outcomes. We examined the plasm glucose and insulin level in both fasting and postprandial condition after the oral glucose tolerance test (OGTT) and calculated the area under the curve of glucose (AUGG) and insulin (AUCI) as well as the homeostasis model assessment for insulin resistance and β-cell function (HOMA-IR and HOMA-β).Results: Both the simple RPL group and the complicated group had significantly higher fasting insulin (FINS), HOMA-IR and HOMA-β than the control group. The simple RIF group had the lowest level of FINS, HOMA-IR and HOMA-β. The incidences of IR were significantly higher in both the simple RPL group and the complicated group than the other two groups. After adjusted for age and waist-hip ratio (WHR), the simple RIF group had the highest fasting plasma glucose (FPG) [adjusted-mean (95%CI), 5.20 (5.09-5.33) mmol/L] and lower FINS [adjusted-mean (95%CI), 10.77 (9.25-12.29) mU/L] and HOMA-β [adjusted-mean (95%CI), 127.76 (83.56-171.97)]; the simple RPL group had the highest FINS [adjusted-mean (95%CI), 12.09 (11.21-12.98) mU/L] and HOMA-β [adjusted-mean (95%CI), 189.74 (164.29-215.18)] and a lower FPG [adjusted-mean (95%CI), 5.03 (4.97-5.10) mmol/L]. The FINS tended to increase with times of implantation failure among those patients with implantation failure fewer than six times. However, patients with more than six times implantation failure had extremely low FINS when compared with those with a history of five or six times (P<0.05). Conclusion: In patients undergoing ART, insulin resistance may be a common etiopathogenesis of RPL and RIF and insulin secretion impairment may be related to RIF.

Long-term out come in patients undergoing liver transplantation (OLT) for cryptogenic cimhosis (CC): A case control study

2001 ◽  
Vol 120 (5) ◽  
pp. A372-A372
Author(s):  
S KHAN ◽  
J EASON ◽  
G LOSS ◽  
A MASON ◽  
S NAIR
Keyword(s):  
Liver Transplantation ◽  
Case Control Study ◽  
Case Control ◽  
Control Study

Outcome of jejunoileal Crohn's disease: A case-control study

2001 ◽  
Vol 120 (5) ◽  
pp. A282-A282
Author(s):  
T HIGUERO ◽  
C MERLE
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Case Control Study ◽  
Case Control ◽  
Control Study
Sign in / Sign up

Export Citation Format

Share Document