scholarly journals Impaired Myeloid-derived Suppressor Cells Are Associated With Recurrent Implantation Failure: A Case-Control Study

2020 ◽  
Author(s):  
Huanhuan Jiang ◽  
Mengting Zhu ◽  
Peipei Guo ◽  
Kaihuan Bi ◽  
Zhimin Lu ◽  
...  
Keyword(s):  
T Cells ◽  
Case Control Study ◽  
Transforming Growth Factor ◽  
Suppressor Cells ◽  
Case Control ◽  
No Production ◽  
Implantation Failure ◽  
Myeloid Derived Suppressor Cells ◽  
Recurrent Implantation Failure ◽  
Control Study

Abstract Background: Studies have reported that myeloid-derived suppressor cells (MDSCs) contribute to maintain pregnancy. The aim of this case-control study was to test whether there is a dysregulation of peripheral MDSCs in recurrent implantation failure (RIF).Methods: 26 RIF patients and 30 controls were recruited. Flow cytometry was applied to characterize polymorphonuclear (PMN)-MDSCs, monocytic-MDSCs (M-MDSCs), effector T cells (Teffs) and regulatory T cells (Tregs) in blood. ELISA was used to define MDSCs correlative cytokines and chemokines in serum from all patients.Results: Compared with controls, RIF patients showed significant reductions of blood PMN-MDSCs, M-MDSCs, Tregs and NO production by PMN-MDSCs, whereas the expression of ζ chain on CD4+T cell receptor (TCR) and CD8+TCR displayed a remarkable upregulation in RIF patients. Moreover, RIF patients presented a lower concentration of serum chemokine (C-C motif) ligand (CCL) 5 and transforming growth factor (TGF)-β than those from controls. Furthermore, the level of TCR ζ chain on CD4+ and CD8+ Teffs was negatively correlated not only with the percentage of PMN-MDSCs, but also with the amount of NO produced by PMN-MDSCs. The frequency of PMN-MDSCs had positive correlations with the concentration of CCL5 and TGF-β.Conclusions: This study indicated that the dysregulation of MDSCs might impair maternal-fetal immune balance thus resulting in RIF.

scholarly journals Factor V H1299r (Hr2) Heterozygosity: A Risk Factor For Recurrent Implantation Failure Particularly In Non-Carriers For Factor V Leiden Mutation-A Case-Control Study

2021 ◽  
pp. 1-5
Author(s):  
A. Seval Ozgu-Erdinc ◽  
Pınar Gulsen Coban ◽  
Nafiye Yilmaz ◽  
Zuhal Candemir ◽  
Saynur Yilmaz ◽  
...  
Keyword(s):  
Case Control Study ◽  
Gene Mutations ◽  
Case Control ◽  
Factor V ◽  
Implantation Failure ◽  
Recurrent Implantation Failure ◽  
Factor V Leiden Mutation ◽  
History Of ◽  
Fv Leiden ◽  
Control Study

Objective: The association between recurrent implantation failure and thrombophilia is still controversial depending on the published reports with conflicting results. In this study, we aimed to assess the clinical relevance of screening women with recurrent implantation failure for some thrombophilic variants including factor V H1299R (FV HR2) haplotype. Study Design: A total of 279 women were recruited in this case-control study. 229 women with a history of recurrent implantation failure and 50 fertile control with no history of pregnancy losses were screened for eight specific gene mutations, regarding factor V G1691A gene (FV Leiden), FV HR2, factor II prothrombin G20210A, factor XIII V34L, PAI-1 4G/5G, MTHFR C677T, MTHFR A1298C and A3 haplotype of the endothelial cell protein C receptor gene. Results: Recurrent implantation failure group displayed a significantly higher prevalence of FV HR2 heterozygosity than fertile controls while the frequency of FV Leiden mutation was comparable between groups (p=0.011; p=0.619). Additionally, the difference in the prevalence of other specific or total gene mutations among women with recurrent implantation failure was also insignificant. Discussion: The primer outcome of this study was the co-existence of the higher prevalence of FV HR2 haplotype and the insignificant percentage of FV Leiden mutation in women with recurrent implantation failure. Thus, we emphasize that the HR2 haplotype may be associated with recurrent implantation failure particularly in non-carriers for FV Leiden mutation. In the necessity of screening for thrombophilia in recurrent implantation failure, HR2 haplotype should be involved in the searched gene panel particularly in the absence of FV Leiden mutation. Further large-scale prospective studies are needed to investigate whether screening or treatment for HR2 haplotype has any detrimental impact on implantation success in cases of recurrent implantation failure.

scholarly journals Analysis of 14-bp HLA-G polymorphism in middle-eastern women with recurrent implantation failure

2020 ◽  
Vol 9 (12) ◽  
pp. 4820
Author(s):  
Rand Sulaiman ◽  
Thara Mohamad ◽  
Marwan Alhalabi
Keyword(s):  
Case Control Study ◽  
Middle Eastern ◽  
Human Leukocyte ◽  
Case Control ◽  
Control Group ◽  
Polymorphism Analysis ◽  
Implantation Failure ◽  
Recurrent Implantation Failure ◽  
Significant Difference ◽  
Control Study

Background: Implantation appears to be the major rate-limiting step in assisted reproductive techniques success. Recurrent implantation failure (RIF) is a common condition, being the failure to achieve a clinical pregnancy after transfer of four or more good-quality embryos in a minimum of two IVF cycles in a woman under the age of 40. Human leukocyte antigen G (HLA-G) is thought to play a key role in implantation by modulating cytokine secretion to enable the semi-allogeneic embryo to implant into the maternal endometrium. The aim of this study was to investigate the association between the 14-bp ins/del HLA-G polymorphism and rates of RIF. No similar studies were conducted nationally or regionally.Methods: The study was designed as a case-control study. A proportion of 30 patients with RIF were selected as the experimental group. The control group included 15 patients with at least one pregnancy following IVF procedure. Peripheral blood samples were collected from both groups. DNA was extracted and the polymorphism analysis was performed using (AMPLI - HLA-G polymorphism) kit, then PCR products were electrophoresed on 2% agarose gel. statistical analysis was performed using SPSS statistics software.Results: A total of 45 middle-eastern patients were recruited in this case-control study. High frequency of the polymorphism was found in both case (76.6%) and control (86.67%) groups, but with no significant difference between the two groups (p=0.429).Conclusions: There is no correlation between the 14-bp ins/del HLA-G polymorphism and RIF, thus it may be considered as a normal polymorphism and might not affect the level of HLA-G.

scholarly journals The relationship between recurrent implantation failure and sexual function in infertile Iranian women: A case-control study

2021 ◽  
Author(s):  
Samira Ghorbani ◽  
Khadijeh Hekmat ◽  
Parvin Abedi ◽  
Saeed Ghanbari ◽  
Narjes Dibavand
Keyword(s):  
Sexual Function ◽  
Case Control Study ◽  
Case Control ◽  
Implantation Failure ◽  
Iranian Women ◽  
Recurrent Implantation Failure ◽  
Chi Square ◽  
Infertile Women ◽  
The Relationship ◽  
Control Study

Abstract Background Recurrent implantation failure affects 3–5% of women, which means failing to implant after two or more high-quality embryo transfer cycles. The aim of this study was to assess the relationship between recurrent implantation failure and sexual function in infertile Iranian women. Methods This was a case-control study of 180 infertile Iranian women (90 infertile women with recurrent implantation failure and 90 infertile women with no implantation failure). A demographic questionnaire and the Female Sexual Function Index were used for data collection. Data analyzed by means of chi-square, independent t-test and linear regression. Results The mean scores of different domains of sexual function including (desire, lubrication, arousal, orgasm, pain, satisfaction) were significantly lower in the group with RIF compared to the group without RIF. The total score of sexual function was significantly lower in the RIF group than in the RIF-free group (23.11 ± 2.24, vs. 25.99 ± 2.35, p < 0.001). Women in the RIF group were 2.65 times more likely to have a low score of sexual function compared to the women with no RIF (p < 0.001). Conclusion Women experiencing recurrent implantation failure may be at particular risk for reduced sexual function. Sexual function issues should be treated as an important component of comprehensive care.

scholarly journals Consistent Role of Insulin Resistance in Recurrent Pregnancy Loss and Recurrent Implantation Failure: A Case-control Study

2021 ◽  
Author(s):  
Miao Ding ◽  
Fengyi He ◽  
Xiaojia Li ◽  
Sushi Jiang ◽  
Yacong Cao ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Case Control Study ◽  
Case Control ◽  
Model Assessment ◽  
Implantation Failure ◽  
Recurrent Implantation Failure ◽  
Control Study

Abstract Background: To explore the role of insulin resistance (IR) in patients with recurrent pregnancy loss (RPL) and/or recurrent implantation failure (RIF) treated with assisted reproductive technology (ART).Methods: We conducted a case-control study in a tertiary hospital from 2012 to 2018, We included 212 cases of simple RPL (only involved in RPL), 123 cases of simple RIF (only involved in RIF), 67 cases involved in both conditions (complicated group). We screened 123 women as the control cohort, who underwent ART due to male infertile, with no adverse pregnant outcomes. We examined the plasm glucose and insulin level in both fasting and postprandial condition after the oral glucose tolerance test (OGTT) and calculated the area under the curve of glucose (AUGG) and insulin (AUCI) as well as the homeostasis model assessment for insulin resistance and β-cell function (HOMA-IR and HOMA-β).Results: Both the simple RPL group and the complicated group had significantly higher fasting insulin (FINS), HOMA-IR and HOMA-β than the control group. The simple RIF group had the lowest level of FINS, HOMA-IR and HOMA-β. The incidences of IR were significantly higher in both the simple RPL group and the complicated group than the other two groups. After adjusted for age and waist-hip ratio (WHR), the simple RIF group had the highest fasting plasma glucose (FPG) [adjusted-mean (95%CI), 5.20 (5.09-5.33) mmol/L] and lower FINS [adjusted-mean (95%CI), 10.77 (9.25-12.29) mU/L] and HOMA-β [adjusted-mean (95%CI), 127.76 (83.56-171.97)]; the simple RPL group had the highest FINS [adjusted-mean (95%CI), 12.09 (11.21-12.98) mU/L] and HOMA-β [adjusted-mean (95%CI), 189.74 (164.29-215.18)] and a lower FPG [adjusted-mean (95%CI), 5.03 (4.97-5.10) mmol/L]. The FINS tended to increase with times of implantation failure among those patients with implantation failure fewer than six times. However, patients with more than six times implantation failure had extremely low FINS when compared with those with a history of five or six times (P<0.05). Conclusion: In patients undergoing ART, insulin resistance may be a common etiopathogenesis of RPL and RIF and insulin secretion impairment may be related to RIF.

Transforming Growth Factor-α and Nonsyndromic Cleft Lip with or without Palate in Brazilian Patients: Results of a Large Case-Control Study

2004 ◽  
Vol 41 (4) ◽  
pp. 387-391 ◽  
Author(s):  
Maria Rita Passos-Bueno ◽  
Dinamar A. Gaspar ◽  
Tânia Kamiya ◽  
Graziela Tescarollo ◽  
Daniel Rabanéa ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cleft Lip ◽  
Case Control Study ◽  
Transforming Growth Factor ◽  
Case Control ◽  
Genotype Distribution ◽  
Chi Square ◽  
Transforming Growth Factor Α ◽  
Factor Α ◽  
Control Study

Objective Transforming growth factor-α (TGFA) was the first gene suggested to be associated with nonsyndromic cleft lip, cleft palate, or both (CL/ P). There are, however, still controversies of the effect of TGFA on the predisposition of this malformation. To contribute to a better understanding of the role of this gene in the occurrence of CL/P we undertook a case-control study including patients and controls ascertained in different regions of the country. Design We examined the C2/TaqI variant of the TGFA gene in 536 patients with nonsyndromic CL/P and 412 controls. The TGFA genotype frequencies in patients were compared with controls using chi-square or Fisher exact test. DNA, obtained from peripheral blood or buccal swabs, was genotyped for the TaqI polymorphism of TGFA. Setting The probands and corresponding controls were ascertained in different centers of Brazil, partly representing the ethnic admixture of our population. Results The TGFA genotype distribution was very similar in patients with CL/P ascertained in the three different regions of Brazil. However, a discrete difference was observed between controls of Säo Paulo and Ceará (chi-square = 3.605; p = 0.058), with a lower value of the C2/Taq allele frequency in controls of CE (0.04). These data reinforce that this polymorphic system is heterogenous among different ethnic groups. In addition, no evidence was found for an association of TGFA with CL/P in this case-control study. Conclusion These data further suggest that TGFA is not a relevant modifier locus for the occurrence of CL/P.

Association between Transforming Growth Factor Alpha TaqI Polymorphism and Susceptibility to the Nonsyndromic Cleft Lip and/or Palate in an Iranian Population: A Case Control Study

2020 ◽  
Author(s):  
Abdolhamid Amooee ◽  
Seyed Mohammadreza Niktabar ◽  
Mohammad Javad Akbarian-Bafghi ◽  
Majid Morovati-Sharifabad ◽  
Mohamad Hosein Lookzadeh ◽  
...  
Keyword(s):  
Allele Frequency ◽  
Cleft Lip ◽  
Case Control Study ◽  
Transforming Growth Factor ◽  
Case Control ◽  
Iranian Population ◽  
Case Control Studies ◽  
Increased Risk ◽  
Significant Difference ◽  
Control Study

Background: The TGF-α TaqI C >T polymorphism is a well-characterized variant for nonsyndromic cleft lip and/or palate (NS CL/P), but it has shown inconsistent results of association with nonsyndromic CL/P across a number of studies. Thus, we have performed this case-control study to clarify the association between the TGF-α TaqI C >T polymorphism and NS CL/P risk.   Methods: One-hundred ten cases with NSCL/P and 110 controls were recruited to the current study. We have genotyped the TGF-α TaqI C >T polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The odds ratio (OR) and 95% confidence interval (CI) were applied for strength of association TGF-α TaqI C >T polymorphism with NSCL/P.   Results: The TGF-α TaqI C >T polymorphism CC, CT and TT genotypes frequencies in the NSCL/P cases were 30.9%, 57.3% and 11.8%, respectively while the corresponding frequencies in the controls were 37.3%, 52.7% and 10.0%, respectively. The frequency of C and T alleles in the case were 59.5% and 40.5%, respectively while the corresponding allelic frequencies in the controls were 63.6% and 36.4%. There was no significant difference in the genotype and allele frequency for TGF-α TaqI C >T polymorphism between cases and controls. The minor allele frequency (MAF) of TGF-α TaqI C >T polymorphism among healthy controls was 0.36.   Conclusion: Our study indicates that the TGF-α TaqI C>T polymorphism was not significantly associated with increased risk of NS CL/P in the Iranian population. However, our results still need to be confirmed by further large and well-designed case-control studies.

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