Stapled Porcine Pericardium Displays Lower Infectivity In Vitro Than Native and Sutured Porcine Pericardium

[abstFig src='/00280004/16.jpg' width='300' text='The pericardium grasp mechanism for pericardiocentesis' ] Complication ratio of the pericardiocentesis was reported 4.7%, due to the procedure using needle. To decrease complication of the pericardiocentesis, purposes of this study was to develop a new pericardiocentesis assist device without needle. The proposed device was able to aspirate fluid inside the pericardial space by grasping and cutting the pericardium. To cut the pericardium, the device needed to grasp the pericardium surely. In this paper, we designed pericardium grasping mechanism. The result of the structural analysis using the finite element method showed proposed grasping mechanism could keep grasping force of 120 N and pericardium elastic force of 2.7 N without fracture. Results of in vitro porcine pericardium grasping experiment using a proposed device model showed that the pericardium grasping force was elucidated to need more than 7.5 N, while the pericardium elastic force was 2.44 N. The proposed pericardium grasping mechanism could grasp a porcine pericardium securely without fracture.

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Anti-inflammatory effect of adipose-derived stem cells in the treatment of pelvic organ prolapse

Journal of International Medical Research ◽

10.1177/0300060519873472 ◽

2019 ◽

Vol 47 (12) ◽

pp. 6303-6314

Author(s):

Su Wang ◽

Jian Gao ◽

Maohuai Wang ◽

Liquan Chen ◽

Xiaowei Zhang ◽

...

Keyword(s):

Stem Cells ◽

Pelvic Organ Prolapse ◽

Adipogenic Differentiation ◽

Pelvic Organ ◽

Tissue Growth ◽

Collagen I ◽

Adipose Derived Stem Cells ◽

Porcine Pericardium

Objective This study investigated the effect of recombinant human connective tissue growth factor (hCTGF) on rat adipose-derived stem cells (ADSCs) and explored the feasibility of using ADSCs to treat pelvic organ prolapse. Methods ADSCs were isolated from rat inguinal adipose tissue and characterized by flow cytometry and for osteogenic and adipogenic differentiation. ADSCs were treated with recombinant hCTGF and qRT-PCR was performed to detect collagen I and III expression on post-treatment days 7, 14, and 28. Osteogenic and adipogenic differentiation of ADSCs was performed to evaluate the effect of hCTGF. ADSCs were seeded in biological grafting materials, acellular porcine pericardium (APP) and acellular bovine pericardium (ABP), then implanted in the rat vagina. Histology was performed to observe inflammation among different groups. Results Collagen I and III expression in ADSCs was significantly increased, and the ability to differentiate into osteogenic and adipogenic lineages was diminished after hCTGF treatment. APP and ABP seeded with ADSCs significantly decreased inflammation and protected from degradation in vivo compared with APP and ABP only; ABP seeded with ADSCs had the lowest inflammation. Conclusion hCTGF regulates collagen I and III expression and induces ADSC differentiation in vitro. ADSCs decrease inflammation associated with APP and ABP in vivo.

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In Vitro Evaluation of Acellular Collagen Matrices Derived from Porcine Pericardium: Influence of the Sterilization Method on Its Biological Properties

Materials ◽

10.3390/ma14216255 ◽

2021 ◽

Vol 14 (21) ◽

pp. 6255

Author(s):

Rone Aparecido De Grandis ◽

Larissa Natiele Miotto ◽

Luis Eduardo Genaro ◽

Larissa Migliatti Polli ◽

Ana Maria de Guzzi Plepis ◽

...

Keyword(s):

Ethylene Oxide ◽

Gamma Radiation ◽

In Vitro Assays ◽

Membrane Thickness ◽

Scanning Calorimetry ◽

Collagen Matrices ◽

Sterilization Method ◽

Significant Difference ◽

Porcine Pericardium

The aim of this study were characterize acellular collagen matrices derived from porcine pericardium (PP) and to evaluate their properties after sterilization by ethylene oxide and gamma ray. PP matrices were subjected to alkaline hydrolysis (AH), and samples were characterized for biological stability, membrane thickness measurements, differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). Subsequently, the matrices were frozen, lyophilized and sterilized by ethylene oxide or gamma radiation. For in vitro assays, CHO-K1 cell culture was used and evaluated for cytotoxicity, clonogenic survival assay, genotoxicity and mutagenicity. Analysis of variance (ANOVA) was used, followed by Dunnett’s post-test, with a significance level of 5%. After AH, there was no significant change in matrix thickness. The relative biodegradability of the material after implantation was observed. Morphology and dimensions had small changes after AH. As for cell viability, none of the tested matrices showed a statistically significant difference (p > 0.05; Dunnett) regardless of the sterilization method. Furthermore, it was found that PP matrices did not interfere with the proliferation capacity of CHO-K1 cells (p > 0.05; Dunnett). As for genotoxicity, when sterilized with ethylene oxide (NP, P12 and P24), it showed genotoxic potential, but it was not genotoxic when sterilized by gamma radiation. No mutagenic effects were observed in either group. PP-derived collagen matrices hydrolyzed at different times were not cytotoxic. It is concluded that the best method of sterilization is through gamma radiation, since no significant changes were observed in the properties of the PP matrices.

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ID:2052 MANUFACTURING BIOLOGICAL MEMBRANE FROM BOVINE PERICARDIUM EXTRACELLULAR MATRIX TOWARD THE PERIODONTAL APPLICATION

Biomedical Research and Therapy ◽

10.15419/bmrat.v4is.286 ◽

2017 ◽

Vol 4 (S) ◽

pp. 82

Author(s):

My Nguyen Thi Ngoc

Keyword(s):

Biological Membrane ◽

Human Fibroblasts ◽

Bovine Pericardium ◽

Membrane Properties ◽

Collagen Membrane ◽

Gingival Fibroblasts ◽

In Vitro Degradation ◽

In Vitro Biocompatibility ◽

Porcine Pericardium

Collagen membrane that was mainly from the bovine and porcine pericardium has been studied and widely applied for most manufacturers of bioprothetic materials, especially periodontal therapeutics. As many studied, bovine pericardium has higher collagen content than that from porcine, although both membranes have no significant different in calcification when treated with glutaraldehyde. In this study, acellular bovine was used to fabricated a biomembrane for periodontal reconstruction and regeneration. Acellular bovine pericardium was treated with glutaraldehyde at different concentrations and time points. The treatment of 0,1% glutaraldehyde for 6 hours was determined as the optimal fabrication protocol according to membrane properties, including thickness (0.2mm-0.5mm), tensile strength (5MPa-12MPa), tensile strain (15%-50%), around 5µm pore size, limited in vitro degradation (1%-5%), which met all criteria parameters for periodontal treatment. The fabricated membrane presented in vitro biocompatibility to human fibroblasts according to ISO10993-5. Moreover, the membrane promoted the in vitro migration of humane gingival fibroblasts, which indicated the application as a guided biomembrane in periodontal reconstuction and regeneration.

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Accelerated in vitro recellularization of decellularized porcine pericardium for cardiovascular grafts

Biomedical Materials ◽

10.1088/1748-605x/abbdbd ◽

2020 ◽

Author(s):

Elena Filova ◽

Marie Steinerova ◽

Martina Travnickova ◽

Jarmila Knitlova ◽

Jana Musilkova ◽

...

Keyword(s):

Porcine Pericardium

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Accelerated in vitro-calcification of potential urethane based heart valve replacement materials

Current Directions in Biomedical Engineering ◽

10.1515/cdbme-2018-0054 ◽

2018 ◽

Vol 4 (1) ◽

pp. 221-224 ◽

Cited By ~ 2

Author(s):

Stefan Oschatz ◽

Stefanie Kohse ◽

Volkmar Senz ◽

Thomas Eickner ◽

Klaus-Peter Schmitz ◽

...

Keyword(s):

Reference Material ◽

Valve Replacement ◽

Heart Valve ◽

Polyamide 6 ◽

Heart Valve Replacement ◽

Applied Method ◽

Polycarbonate Urethane ◽

Short Time ◽

Porcine Pericardium

AbstractPolycarbonate urethane and polyether urethane nonwovens as promising representatives of novel polymer heart valve materials were analysed regarding the susceptibility to calcification in comparison to porcine pericardium and polyamide 6. The applied method represents an accelerated calcification out of a metastable solution in short time with significant precipitates on the reference material. As our results show, urethane based nonwoven structures exhibit considerably lower susceptibility to calcification compared to pericardium as widely established material for leaflet design.

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Ultrastructural Investigations of the Contractile Filaments of Amoebae

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100050950 ◽

1974 ◽

Vol 32 ◽

pp. 188-189

Author(s):

P.L. Moore

Keyword(s):

Cytoplasmic Streaming ◽

Three Dimensional ◽

Freeze Fracture ◽

Amoeboid Movement ◽

Different Types ◽

Chemical Conditions ◽

Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

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In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100050676 ◽

1974 ◽

Vol 32 ◽

pp. 132-133

Author(s):

John J. Wolosewick ◽

John H. D. Bryan

Keyword(s):

Endoplasmic Reticulum ◽

Smooth Endoplasmic Reticulum ◽

Nuclear Ring ◽

Rough Er ◽

Distal Direction ◽

In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

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Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053425 ◽

1982 ◽

Vol 40 ◽

pp. 142-145

Author(s):

E. J. Kollar

Keyword(s):

Connective Tissue ◽

Oral Mucosa ◽

Basal Lamina ◽

Functional Derivatives ◽

Specific Source ◽

Dental Epithelium ◽

Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

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