Human meibomian gland epithelial cell culture models: Current progress, challenges, and future directions

Author(s):  
Minh Anh Thu Phan ◽  
Michele C. Madigan ◽  
Fiona Stapleton ◽  
Mark Willcox ◽  
Blanka Golebiowski
2014 ◽  
Vol 87 (3) ◽  
pp. 480-488 ◽  
Author(s):  
Pall Thor Ingvarsson ◽  
Ida Svahn Rasmussen ◽  
Michelle Viaene ◽  
Przemyslaw Jedrzej Irlik ◽  
Hanne Mørck Nielsen ◽  
...  

2014 ◽  
Vol 25 (4) ◽  
pp. 377-387 ◽  
Author(s):  
David Freestone ◽  
Michael A. Cater ◽  
M. Leigh Ackland ◽  
David Paterson ◽  
Daryl L. Howard ◽  
...  

2015 ◽  
Vol 332 (1) ◽  
pp. 89-101 ◽  
Author(s):  
Polina Ilina ◽  
Susanna Partti ◽  
Johanna Niklander ◽  
Marika Ruponen ◽  
Yan-Ru Lou ◽  
...  

Metallomics ◽  
2016 ◽  
Vol 8 (9) ◽  
pp. 941-950 ◽  
Author(s):  
David Freestone ◽  
Delphine Denoyer ◽  
Matthew Jakab ◽  
M. Leigh Ackland ◽  
Michael A. Cater ◽  
...  

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Benjamin L. Duell ◽  
Allan W. Cripps ◽  
Mark A. Schembri ◽  
Glen C. Ulett

Countless in vitro cell culture models based on the use of epithelial cell types of single lineages have been characterized and have provided insight into the mechanisms of infection for various microbial pathogens. Diverse culture models based on disease-relevant mucosal epithelial cell types derived from gastrointestinal, genitourinary, and pulmonary organ systems have delineated many key host-pathogen interactions that underlie viral, parasitic, and bacterial disease pathogenesis. An alternative to single lineage epithelial cell monoculture, which offers more flexibility and can overcome some of the limitations of epithelial cell culture models based on only single cell types, is coculture of epithelial cells with other host cell types. Various coculture models have been described, which incorporate epithelial cell types in culture combination with a wide range of other cell types including neutrophils, eosinophils, monocytes, and lymphocytes. This paper will summarize current models of epithelial cell coculture and will discuss the benefits and limitations of epithelial cell coculture for studying host-pathogen dynamics in infectious diseases.


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