Influence of guanidination on apparent ileal digestibility of amino acids in pigs fed diets with soybean meal, rapeseed meal or peas as a protein source

2008 ◽  
Vol 116 (1-3) ◽  
pp. 300-308 ◽  
Author(s):  
William R. Caine ◽  
Willem C. Sauer ◽  
Guishan S. Huang ◽  
Gerd Diebold ◽  
Margit Schollenberger ◽  
...  
2021 ◽  
Vol 30 (2) ◽  
Author(s):  
Sini Perttilä ◽  
Taina Jalava ◽  
Marketta Rinne ◽  
Gabriel Da Silva Viana ◽  
Jarmo Valaja

The apparent (AID) and(SID) ileal amino acid digestibilities in wheat, soybean meal, and rapeseed meal were determined with Ross 308 broiler chicken (n = 64) using the slaughter technique with chromium mordanted straw as an indigestible marker. The recovery of endogenous amino acids at the distal ileum was determined with a protein-free diet and it was used to calculate the SID digestibilities of the studied feed ingredients. The mean amino acid AID and SID were higher in soybean meal and wheat than in rapeseed meal (p<0.05). The mean amino acid flow measured from the ileum was the highest in rapeseed meal, intermediate in soybean meal, and the lowest in wheat (p<0.05). The main amino acids in the basal endogenous secretion were aspartic and glutamic acid and the lowest ones present were methionine and histidine (p<0.05). The difference between amino acid AID and SID values varied among ingredients and was higher in wheat than in soybean meal and rapeseed meal (p<0.05).


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 175-175
Author(s):  
Élisabeth Chassé ◽  
Frédéric Guay ◽  
Marie-Pierre Létourneau-Montminy

Abstract This study was conducted to determine the effect of pelleting on the digestibility of corn-soybean meal-based diet in growing pigs. Two trials with 6 pigs cannulated at the distal ileum were conducted. In each trial, pigs were assigned to each treatment following a crossover design. In each experiment, the same diet, composed of corn and soybean meal with 10% wheat from two different feed mills, was served in pellet or mash form. Pelleting allowed an increase in digestibility in one of the trials. Apparent ileal digestibility (AID) of dry matter (DM), crude protein (CP) and digestible energy (DE) were improved with pelleting by 8, 12 and 9% (P&lt; 0.01). The AID of amino acids (AA) was also improved (P&lt; 0.05). Apparent total tract digestibility (ATTD) was increased by pelleting in DM, CP and DE by 5, 7 and 6% respectively (P&lt; 0.01). The digestibility of the mash diet in experiment 1 was lower than in the pelleted diet in the experiment 1 and both diets in experiment 2 as shown by the interaction Pelleting X Trial which was significant for the AID and ATTD of DM, CP and DE (P&lt; 0.01). Therefore, in experiment 1, pelleting allows to improve the digestibility of diet to the same level as in experiment 2. The AID of CP was higher by 37% in the mash diet from the second experiment compared to the one in the first experiment. Even though the same ingredients were chosen in the two experiments, this shows the variability in digestibility existing between different feed mills and ingredient sources. This difference was not observed in pelleted diets. The results obtained in these two trials show that pelleting can reduce the variability of digestibility and then give a good digestibility of diets even if the ingredients are of different quality or sources.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 61-61
Author(s):  
Chan Sol Park ◽  
Ayodeji S Aderibigbe ◽  
Gary Hayen ◽  
Olayiwola Adeola

Abstract Two experiments were conducted to evaluate the digestible energy (DE), metabolizable energy (ME), and standardized ileal digestibility (SID) of amino acids (AA) in dried yeast (DY) and soybean meal (SBM) fed to pigs. In Exp. 1, 30 barrows with an initial body weight (BW) of 20.7 ± 1.01 were assigned to 5 diets in a randomized complete block design with period and BW as blocking factors. A basal diet was prepared to contain corn, canola meal, and soybean oil as energy-contributing ingredients. Four additional diets were prepared by adding 5 or 10 g/kg DY or SBM at the expense of energy-contributing ingredients in the basal diet to estimate the DE and ME in test ingredients by regression analysis. On a dry matter basis, estimated DE and ME in DY were 4,022 and 3,352 kcal/kg, respectively, and those in SBM were 3,876 and 3,601kcal/kg, respectively. There was no difference in estimated DE or ME between DY and SBM. In Exp. 2, 21 barrows (initial BW = 20.0 ± 1.31 kg) surgically fitted with T-cannulas at the distal ileum were assigned to 3 diets in a randomized complete block design with BW as a blocking factor. Two diets were prepared to contain DY or SBM as the sole source of nitrogen, and a nitrogen-free diet was prepared to determine the basal ileal endogenous losses of AA. The SID of AA, except for Gly and Pro, in SBM were greater (P &lt; 0.05) than in DY. The SID of indispensable AA in DY ranged from 64.7% for Thr to 86.1% for Arg, whereas those in SBM ranged from 84.8% for Thr to 92.3% for Arg. In conclusion, energy values in DY was comparable with SBM, but the SID of most AA in DY were less than in SBM.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 200-201
Author(s):  
Hyunjun Choi ◽  
Sun Jong You ◽  
Beob Gyun G Kim

Abstract The objective was to determine the influence of amino acid (AA) supplementation during the adaptation period on the ileal digestibility of crude protein and AA in corn and soybean meal (SBM). Six barrows with an initial body weight of 30.9 ± 2.6 kg fitted with a T-cannula in the distal ileum were assigned to a 6 × 6 Latin square design with 6 dietary treatments and 6 periods. Two experimental diets contained corn or SBM as the sole source of AA and an N-free diet was additionally prepared. For AA supplementation groups, an AA mixture consisted of Gly, Lys, Met, Thr, Trp, Ile, Val, His, and Phe was added to the corn diet and the N-free diet at the expense of cornstarch, and an AA mixture of Lys, Met, and Thr was added to the SBM diet. All diets contained 0.5% of chromic oxide. The 6 experimental diets were fed to the pigs for 4 and half days, and the 3 diets containing AA mixture were switched to the respective diets without AA mixture during the following 2 and half days. Ileal digesta were collected during the last 2 days. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Arg and Trp in corn (P &lt; 0.05), but did not affect that in SBM. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Pro and Gly regardless of feed ingredient (P &lt; 0.05), but did not affect that of other AA. All AA except Pro in corn and SBM were unaffected by the addition of AA mixture during the adaptation period. In conclusion, the addition of amino acid during the adaptation period does not affect the standardized ileal digestibility of indispensable amino acids in feed ingredients.


2015 ◽  
Vol 153 (5) ◽  
pp. 943-953 ◽  
Author(s):  
S. C. ÁVILA ◽  
G. V. KOZLOSKI ◽  
T. ORLANDI ◽  
M. P. MEZZOMO ◽  
S. STEFANELLO

SUMMARYFour Holstein steers (297 ± 56 kg of body weight (BW)) fitted with duodenal cannula and rumen catheter, were housed in metabolism cages and used in a 4 × 4 Latin Square trial to evaluate the effect of both protein source andAcacia mearnsiitannin extract on digestibility, ruminal fermentation, rumen microbial protein synthesis, N utilization and on duodenal flow of individual amino acids. The diet was offered at restricted amount of 25 g of dry matter (DM)/kg BW and consisted of maize silage plus concentrate, in a proportion of 0·7:0·3 (DM basis) respectively. Concentrate was formulated with either soybean meal or canola meal as protein source, with or without 50 g/kg ofA. mearnsiitannin extract (i.e. 15 g/kg of total dietary DM). There was no effect of protein source on most variables. The apparent and true organic matter (OM) digestibilities, as well as neutral detergent fibre (NDF) digestibility were negatively affected by tannin extract inclusion without, however, affecting digestible OM intake. The amount of nitrogen (N) excreted in faeces increased whereas the urinary N excretion decreased in tannin extract treatments. No interaction time × treatment was detected for any rumen variable and no treatment effect was observed for rumen fluid pH and reducing sugars concentration. Rumen fluid concentration of ammonia N was lower for the canola meal plus tannin extract treatment. Rumen concentration ofα-amino compounds was not affected by tannin extract but was higher when canola meal was the protein source. The duodenal flow of OM, total N,α-amino N and non-ammonia non-microbial N increased with tannin extract inclusion, whereas the duodenal flow of microbial N was not affected by treatment. For both protein sources, the amount of most individual amino acids flowing to the duodenum increased due to tannin extract addition. In conclusion, the dietary inclusion of 15 g/kg DM of tannin extract fromA. mearnsiiimproved the amino acid supply independently of whether the protein source was canola meal or soybean meal, without affecting the amino acid profile, to steers fed maize silage plus concentrate, with a minor but significant impact on OM digestibility.


Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1774
Author(s):  
Zhengke Wu ◽  
Jiao Liu ◽  
Jiang Chen ◽  
Shoaib Ahmed Pirzado ◽  
Yang Li ◽  
...  

Rapeseed meal (RSM) is a common protein ingredient in animal diets, while the proportion of RSM in diets is limited because of its anti-nutritional factors. Fermentation based on mixed microbial strains appears to be a suitable approach to improve the nutritive value of rapeseed meal in animal feed. In this study, we evaluated the effects of fermentation on the apparent metabolizable energy (AME) values and standardized ileal digestibility (SID) of amino acids in RSM fed broilers. The AME and nitrogen-corrected apparent metabolizable energy (AMEn) values of RSM and fermented rapeseed meal (FRSM) were determined by the substitution method, with RSM and FRSM proportionally replacing the energy-yielding components of the basal diet by 30%. Results show that fermentation improved AME and AMEn of RSM from 7.44 to 8.51 MJ/kg and from 7.17 to 8.26 MJ/kg, respectively. In the second experiment, two experimental diets were formulated, with RSM and FRSM being the sole sources of amino acids. A nitrogen-free diet (NFD) was also formulated to determine endogenous amino acids losses (EAAL). Feeding on FRSM resulted in higher (p < 0.05) apparent ileal digestibility (AID) and SID of alanine, valine, isoleucine, leucine, tyrosine, lysine, arginine, and phenylalanine. No significant differences between RSM and FRSM were found for AID and SID of asparagine, histidine, threonine, serine, glutamine, praline, glycine, methionine, and cystine. FRSM had greater AMEn values and SID of amino acids compared to RSM, therefore, FRSM was nutritionally superior to RSM in broiler diets.


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