scholarly journals Effects of Fermentation on Standardized Ileal Digestibility of Amino Acids and Apparent Metabolizable Energy in Rapeseed Meal Fed to Broiler Chickens

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1774
Author(s):  
Zhengke Wu ◽  
Jiao Liu ◽  
Jiang Chen ◽  
Shoaib Ahmed Pirzado ◽  
Yang Li ◽  
...  

Rapeseed meal (RSM) is a common protein ingredient in animal diets, while the proportion of RSM in diets is limited because of its anti-nutritional factors. Fermentation based on mixed microbial strains appears to be a suitable approach to improve the nutritive value of rapeseed meal in animal feed. In this study, we evaluated the effects of fermentation on the apparent metabolizable energy (AME) values and standardized ileal digestibility (SID) of amino acids in RSM fed broilers. The AME and nitrogen-corrected apparent metabolizable energy (AMEn) values of RSM and fermented rapeseed meal (FRSM) were determined by the substitution method, with RSM and FRSM proportionally replacing the energy-yielding components of the basal diet by 30%. Results show that fermentation improved AME and AMEn of RSM from 7.44 to 8.51 MJ/kg and from 7.17 to 8.26 MJ/kg, respectively. In the second experiment, two experimental diets were formulated, with RSM and FRSM being the sole sources of amino acids. A nitrogen-free diet (NFD) was also formulated to determine endogenous amino acids losses (EAAL). Feeding on FRSM resulted in higher (p < 0.05) apparent ileal digestibility (AID) and SID of alanine, valine, isoleucine, leucine, tyrosine, lysine, arginine, and phenylalanine. No significant differences between RSM and FRSM were found for AID and SID of asparagine, histidine, threonine, serine, glutamine, praline, glycine, methionine, and cystine. FRSM had greater AMEn values and SID of amino acids compared to RSM, therefore, FRSM was nutritionally superior to RSM in broiler diets.

2020 ◽  
Vol 98 (7) ◽  
Author(s):  
Maryane S F Oliveira ◽  
Markus K Wiltafsky-Martin ◽  
Hans H Stein

Abstract Two experiments were conducted to test the hypothesis that both the degree of heating and the time that heat is applied will affect the concentration of DE and ME, and the apparent ileal digestibility (AID) and the standardized ileal digestibility (SID) of amino acids (AA) in 00-rapeseed meal (00-RSM) fed to growing pigs. The nine treatments were prepared using a conventional 00-RSM that was either not autoclaved or autoclaved at 110 °C for 15 or 30 min or at 150 °C for 3, 6, 9, 12, 15, or 18 min. In experiment 1, 20 growing barrows with an average initial BW of 21.2 ± 1.2 kg were randomly allotted to the 10 diets in a replicated 10 × 4 Youden square with 10 diets and four periods in each square. A corn-based basal diet and nine diets containing corn and each source of 00-RSM were formulated. Urine and fecal samples were collected for 5 d after 7 d of adaptation. In experiment 2, nine diets contained one of the nine sources of 00-RSM as the sole source of AA, and an N-free diet that was used to measure basal endogenous losses of AA and CP was formulated. Twenty growing barrows with an initial BW of 69.8 ± 5.7 kg had a T-cannula installed in the distal ileum and were allotted to a 10 × 7 Youden square design with 10 diets and 7 periods. Ileal digesta were collected on days 6 and 7 of each 7-d period. Results from the experiments indicated that there were no effects of autoclaving at 110 °C on DE and ME or on AID and SID of AA in 00-RSM, but DE and ME, and AID and SID of AA were less (P &lt; 0.01) if 00-RSM was autoclaved at 150 °C compared with 110 °C. At 150 °C, there were decreases (quadratic, P &lt; 0.05) in DE and ME, and in AID and SID of AA as heating time increased. In conclusion, autoclaving at 110 °C did not affect ME or SID of AA in 00-RSM, but autoclaving at 150 °C had negative effects on ME and SID of AA and the negative effects increased as heating time increased.


2016 ◽  
Vol 40 (5) ◽  
pp. 565-576 ◽  
Author(s):  
Felipe Santos Dalólio ◽  
Luiz Fernando Teixeira Albino ◽  
Horácio Santiago Rostagno ◽  
Diego Ladeira da Silva ◽  
Maurílio de Lucas Xavier Júnior ◽  
...  

ABSTRACT Two experiments were carried out to determine the energy values and the true ileal digestibility of amino acids of nine toasted full-fat soybeans (FFSB), without or with the addition of monocomponent protease. In the first experiment, to determine the energy values, the method of excreta collection was used with 1120 broiler chicks at 14 days old, distributed in randomized blocks in a 10x2 factorial arrangement with nine full-fat soybeans (30% included) plus a control diet (70%) without or with the addition of protease, totaling 20 treatments with 8 replicates and 7 birds per cage. In the second experiment, 1120 broiler chickens from 24 to 28 days of age were distributed in randomized blocks in a 10x2 factor arrangement, with nine full-fat soybeans (40% included) + a protein free diet (60%), without or with the addition of protease, totaling 20 treatments with 8 replicates and 7 birds per cage. Ileal digesta collection was used to determine the true digestibility coefficients and the digestible amino acid content of full-fat soybean. The average values of nitrogen-corrected apparent metabolizable energy (AMEn) and their respective metabolization coefficients (CAMEn) were 3207 kcal/kg and 62.57%, respectively. The average values of digestibility coefficients of crude protein and essential and nonessential amino acids were 86.79, 87.90 and 84.34%, respectively. The inclusion of protease improved (P<0.05) all evaluated parameters. Therefore, its use is recommended in diets containing full-fat soybeans for broiler chickens.


2021 ◽  
Vol 66 (No. 1) ◽  
pp. 29-37
Author(s):  
Júlia Marixara Sousa da Silva ◽  
Nayanne Rodrigues de Oliveira ◽  
Alison Batista Vieira Silva Gouveia ◽  
Rodolfo Alves Vieira ◽  
Ronan Omar Fernandes dos Santos ◽  
...  

Enzymes benefit digestion and absorption of the ingredients and their addition to an animal-origin meal (AOM) can improve its nutritional quality. This research aimed to evaluate the effect of protease on nutrient digestibility, amino acids, and metabolism of AOM energy for broilers. Four hundred and eighty broiler chickens were distributed in a completely randomized design (4 × 2 factorial scheme), eight treatments, six replicates containing 10 birds/replicate. Treatments consisted of poultry viscera meal, swine viscera meal (SVM), bovine meat and bone meal, and basal diet; with and without protease addition. Two tests were performed. In the first test, the total excreta collection method was used with birds at 13 to 20 days of age and 25% of the reference feed was replaced by AOM. In the second test, a protein-free diet was administered to birds at 21 to 24 days of age and AOM replaced 25% of the starch. The inclusion of protease increased the apparent metabolizable energy corrected for nitrogen balance of SVM by 15.99% and the apparent metabolizable crude energy by 5.7%, and it also raised the coefficient of true ileal digestibility of the amino acids in the AOMs by 5.67% on average. The inclusion of protease improved the apparent metabolizable crude energy of AOMs, apparent metabolizable dry matter of bovine meat and bone meal, coefficient of true ileal digestibility of essential amino acids, and apparent metabolizable energy corrected for nitrogen balance of SVM. Dietary supplementation of protease may be a potential strategy to improve the digestibility of amino acids for broilers, a possibility of using animal-origin meals as a protein source of diets.


2020 ◽  
Vol 100 (3) ◽  
pp. 447-454 ◽  
Author(s):  
Haley Leung ◽  
Elijah G. Kiarie

Standardized ileal digestibility (SID) of amino acids (AA) and apparent metabolizable energy corrected for nitrogen (AMEn) in samples of organic corn and soybean meal (SBM) were determined. Conventional corn (CC) and SBM (CSBM) samples were tested for comparison. A total of 560, fourteen-day-old male broiler chickens (Cobb 500) were weighed, placed in cages (10 birds per cage), and allocated to seven (n = 8) semi-purified wheat-starch-based diets. Diets were (1) CC, (2) imported organic corn, (3) local organic corn, (4) CSBM, (5) imported organic SBM (OSBMI), (6) local organic SBM (OSBML), and (7) nitrogen-free wheat starch. Only few differences were observed on SID of AA; SID of lysine was lower (P = 0.002) in organic corn samples relative to CC, and SID of methionine was lower (P = 0.002) in OSBML sample relative to CSBM and OSBMI samples. The AMEn of CC was higher (P < 0.01) than that of organic corn samples. The AMEn of OSBML was higher (P < 0.001) than for CSBM and OSBMI; however, the value for OSBMI was higher (P < 0.001) than for CSBM. In conclusion, utilization of AA in conventional and organic feedstuffs was comparable; however, differences in energy utilization warrant considerations in organic broiler feed formulation.


2014 ◽  
Vol 59 (No. 2) ◽  
pp. 69-83 ◽  
Author(s):  
D.W. Liu ◽  
J.J. Zang ◽  
L. Liu ◽  
N.W. Jaworski ◽  
Z.J. Fan ◽  
...  

Three experiments were conducted to compare raw corn, extruded corn, and dehulled-extruded corn in piglets&rsquo; feeding. In Experiment (Exp.) 1, 8 barrows (19.9 &plusmn; 0.6 kg) were fed experimental diets containing one out of three corn samples and a rice-soybean meal basal diet in a double 4 &times; 4 Latin square design to determine the digestible energy (DE) and metabolizable energy (ME) in the corn samples using the difference method. The DE content in extruded (14.29 MJ/kg) and dehulled-extruded (14.42 MJ/kg) corn was greater (P &lt; 0.05) than in raw corn (13.57 MJ/kg). In Exp. 2, 5 barrows (26.2 &plusmn; 1.3 kg) were fitted with ileal T-cannulas and used in a 5 &times; 5 Latin square design to determine the apparent (AID) and standardized (SID) ileal digestibility of amino acids (AA). The diets comprised the basal diet, the three corn diets from Exp. 1, as well as a nitrogen-free diet to estimate basal endogenous losses of AA. The AID and SID of isoleucine, leucine, lysine, threonine, and valine in dehulled-extruded corn was lower (P &lt; 0.05) than in raw or extruded corn. In Exp. 3, 108 weaned 35&nbsp;days old piglets (8.4 &plusmn; 1.2 kg) were allotted to one of the three diets based on corn type. Weaned pigs fed diets containing extruded or dehulled-extruded corn exhibited reduced (P &lt; 0.05) weight gain and feed intake than pigs fed diets containing raw corn. The diet containing dehulled-extruded corn resulted in a higher incidence of diarrhoea. In summary, extrusion of corn did not result in improvements in digestibility and dehulling corn prior to extrusion appeared to result in heat damage which reduced ileal digestibility of AA. Substitution of raw corn with extruded or dehulled-extruded corn in starter diets formulated to equal quantities of ME and SID AA content did not improve the performance of weaned pigs. &nbsp;


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 61-61
Author(s):  
Chan Sol Park ◽  
Ayodeji S Aderibigbe ◽  
Gary Hayen ◽  
Olayiwola Adeola

Abstract Two experiments were conducted to evaluate the digestible energy (DE), metabolizable energy (ME), and standardized ileal digestibility (SID) of amino acids (AA) in dried yeast (DY) and soybean meal (SBM) fed to pigs. In Exp. 1, 30 barrows with an initial body weight (BW) of 20.7 ± 1.01 were assigned to 5 diets in a randomized complete block design with period and BW as blocking factors. A basal diet was prepared to contain corn, canola meal, and soybean oil as energy-contributing ingredients. Four additional diets were prepared by adding 5 or 10 g/kg DY or SBM at the expense of energy-contributing ingredients in the basal diet to estimate the DE and ME in test ingredients by regression analysis. On a dry matter basis, estimated DE and ME in DY were 4,022 and 3,352 kcal/kg, respectively, and those in SBM were 3,876 and 3,601kcal/kg, respectively. There was no difference in estimated DE or ME between DY and SBM. In Exp. 2, 21 barrows (initial BW = 20.0 ± 1.31 kg) surgically fitted with T-cannulas at the distal ileum were assigned to 3 diets in a randomized complete block design with BW as a blocking factor. Two diets were prepared to contain DY or SBM as the sole source of nitrogen, and a nitrogen-free diet was prepared to determine the basal ileal endogenous losses of AA. The SID of AA, except for Gly and Pro, in SBM were greater (P &lt; 0.05) than in DY. The SID of indispensable AA in DY ranged from 64.7% for Thr to 86.1% for Arg, whereas those in SBM ranged from 84.8% for Thr to 92.3% for Arg. In conclusion, energy values in DY was comparable with SBM, but the SID of most AA in DY were less than in SBM.


2016 ◽  
Vol 16 (4) ◽  
pp. 1059-1072 ◽  
Author(s):  
Andrzej Rutkowski ◽  
Sebastian A. Kaczmarek ◽  
Marcin Hejdysz ◽  
Dorota Jamroz

Abstract The aim of investigations was the estimation of nutritional value of currently cultivated yellow lupine cultivars in raw and extruded form, and their usefulness for broiler chickens. Two experiments were conducted with male Ross 308 chickens. In a digestibility trial 60 fifteen-day-old birds were randomly assigned to three dietary treatments (20 replications in each). Birds were kept in individual pens. Digestibility was calculated using the difference method. From day 16 to 21, diets contained lupine meal in raw or extruded form and the basal diet in the ratio 20:80. Subsequently, the total digestibility of dry matter and crude fat, also apparent nitrogen retention and AMEN value of lupine seeds were determined on chickens fed different forms of lupine. The ileal digestibility of crude protein and amino acids of lupine seeds was also analyzed. In the second experiment the one-day-old birds were randomly assigned to ten dietary treatments (10 replications in each) and were fed diets with increasing amounts of raw or extruded lupine from 10 to 30%. The chickens in control treatments were fed a diet without lupine. Extruded yellow lupine meal was characterized by lower phytic P content in comparison to raw yellow lupine meal. The content of remaining ingredients and antinutritional substances was similar. Yellow lupine seeds post extrusion were characterized by better total crude fat digestibility, nitrogen retention and AMEN values, compared to raw seeds. Higher ileal digestibility was confirmed in numerous amino acids, except lysine, threonine and valine (P≤0.05). By feeding the broilers with diets consisting of 10 to 30% of lupine seeds post extrusion (experiment II), improved apparent fat digestibility, apparent nitrogen retention and AMEN values were achieved in young chickens (P<0.01). Using 10 and 20% of lupine in the diets showed significant positive effects of extrusion on body weight gains, feed intake and feed conversion rate. The performance indices of chickens were drastically decreased by use of 25% ratio of both raw and extruded yellow lupine in the diet. This effect was heightened by a 30% share in feed mixtures.


1982 ◽  
Vol 62 (3) ◽  
pp. 869-875 ◽  
Author(s):  
H. W. HULAN ◽  
F. G. PROUDFOOT

Sorghum grain (SG) contained more crude protein (103.5 g/kg) and ash (17.0 g/kg) but less crude fiber (17.7 g/kg) and total lipid (22.6 g/kg) than corn. The SG contained 0.37% ± 0.02 tannin, had a higher apparent metabolizable energy value than corn or wheat and contained all of the amino acids considered essential for chickens at levels in general between those of corn and wheat. Two experiments were carried out to ascertain the nutritive value of SG as a partial replacement for corn and wheat in practical poultry diets using 320 male and 320 female Cobb chicks in each. Starter (finisher) diets fed from 0–21 days (22–42 days) contained the following levels of SG: 15 (19); 30 (39); 45 (58)%. The diets were isoenergic and isonitrogenous. The inclusion of up to 45% SG in the starter diet and up to 58% in the finisher diet had no significant effect on mortality, live body weight, feed conversion or percentage of grade A carcasses. Key words: Broiler chickens, sorghum grain, milo, diets


2020 ◽  
Vol 100 (4) ◽  
pp. 729-738
Author(s):  
Peili Li ◽  
Zhiqian Lyu ◽  
Lu Wang ◽  
Bingbing Huang ◽  
Changhua Lai

Two experiments were conducted to evaluate the effects of adding multi-enzyme on the available energy concentration and standardized ileal digestibility (SID) of amino acids of double-low rapeseed expellers (RSE) and rapeseed meal (RSM) fed to growing pigs. In Experiment 1, a total of 36 growing barrows were fed six diets in a replicated 3 × 2 factorial design. Diets included a corn–soybean meal basal diet, RSE diet, and RSM diet with or without multi-enzyme (1000 U g−1 of cellulase, 10 000 U g−1 of xylanase, 2000 U g−1 of glucanase, and 10 000 U g−1 of protease). In Experiment 2, a total of 30 crossbred pigs were randomly allotted to five diets consisting of a nitrogen-free diet and four cornstarch-based diets containing RSE or RSM with or without multi-enzyme. Multi-enzyme supplementation significantly improved (P < 0.05) the metabolizable energy (ME) value and apparent total tract digestibility (ATTD) of ether extract, neutral detergent fiber, and acid detergent fiber, and it tended to increase (P < 0.10) the ATTD of gross energy and digestible energy in ingredients. With the exception of phenylalanine, multi-enzyme supplementation increased (P < 0.05) the SID of crude protein (CP) and all amino acids (AA) in ingredients. Multi-enzyme supplementation enhanced fiber degradation and increased the ME value and SID of CP as well as most AA in double-low rapeseed co-products fed to pigs.


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