Tropical fruit pulps decreased probiotic survival to in vitro gastrointestinal stress in synbiotic soy yoghurt with okara during storage

LWT ◽  
2014 ◽  
Vol 55 (2) ◽  
pp. 436-443 ◽  
Author(s):  
Raquel Bedani ◽  
Antonio Diogo Silva Vieira ◽  
Elizeu Antonio Rossi ◽  
Susana Marta Isay Saad
2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Zahaed Evangelista-Martínez ◽  
Erika Anahí Contreras-Leal ◽  
Luis Fernando Corona-Pedraza ◽  
Élida Gastélum-Martínez

Abstract Background Fungi are one of the microorganisms that cause most damage to fruits worldwide, affecting their quality and consumption. Chemical controls with pesticides are used to diminish postharvest losses of fruits. However, biological control with microorganisms or natural compounds is an increasing alternative to protect fruits and vegetables. In this study, the antifungal effect of Streptomyces sp. CACIS-1.5CA on phytopathogenic fungi that cause postharvest tropical fruit rot was investigated. Main body Antagonistic activity was evaluated in vitro by the dual confrontation over fungal isolates obtained from grape, mango, tomato, habanero pepper, papaya, sweet orange, and banana. The results showed that antagonistic activity of the isolate CACIS-1.5CA was similar to the commercial strain Streptomyces lydicus WYEC 108 against the pathogenic fungi Colletotrichum sp., Alternaria sp., Aspergillus sp., Botrytis sp., Rhizoctonia sp., and Rhizopus sp. with percentages ranging from 30 to 63%. The bioactive extract obtained from CACIS-1.5 showed a strong inhibition of fungal spore germination, with percentages ranging from 92 to 100%. Morphological effects as irregular membrane border, deformation, shrinkage, and collapsed conidia were observed on the conidia. Molecularly, the biosynthetic clusters of genes for the polyketide synthase (PKS) type I, PKS type II, and NRPS were detected in the genome of Streptomyces sp. CACIS-1.5CA. Conclusions This study presented a novel Streptomyces strain as a natural alternative to the use of synthetic fungicides or other commercial products having antagonistic microorganisms that were used in the postharvest control of phytopathogenic fungi affecting fruits.


Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 614
Author(s):  
Manoj Kumar ◽  
Sushil Changan ◽  
Maharishi Tomar ◽  
Uma Prajapati ◽  
Vivek Saurabh ◽  
...  

Annona squamosa L. (custard apple) belongs to the family Annonaceae and is an important tropical fruit cultivated in the West Indies, South and Central America, Ecuador, Peru, Brazil, India, Mexico, the Bahamas, Bermuda, and Egypt. Leaves of custard apple plants have been studied for their health benefits, which are attributed to a considerable diversity of phytochemicals. These compounds include phenol-based compounds, e.g., proanthocyanidins, comprising 18 different phenolic compounds, mainly alkaloids and flavonoids. Extracts from Annona squamosa leaves (ASLs) have been studied for their biological activities, including anticancer, antidiabetic, antioxidant, antimicrobial, antiobesity, lipid-lowering, and hepatoprotective functions. In the current article, we discussed the nutritional and phytochemical diversity of ASLs. Additionally, ASL extracts were discussed with respect to their biological activities, which were established by in vivo and in vitro experiments. A survey of the literature based on the phytochemical profile and health-promoting effects of ASLs showed that they can be used as potential ingredients for the development of pharmaceutical drugs and functional foods. Although there are sufficient findings available from in vitro and in vivo investigations, clinical trials are still needed to determine the exact effects of ASL extracts on human health.


2017 ◽  
Vol 66 (2) ◽  
Author(s):  
Porfirio Gutierréz Martínez ◽  
Silvia Bautista-Baños ◽  
Guillermo Berúmen-Varela ◽  
Anelsy Ramos-Guerrero ◽  
Alba María Hernández-Ibañez

LWT ◽  
2019 ◽  
Vol 99 ◽  
pp. 404-410 ◽  
Author(s):  
Douglas Xavier dos Santos ◽  
Alessandro Alberto Casazza ◽  
Bahar Aliakbarian ◽  
Raquel Bedani ◽  
Susana Marta Isay Saad ◽  
...  

2016 ◽  
Vol 17 (2) ◽  
pp. 138
Author(s):  
Liza Octriana

<p>The Potential of Biological Agents to Inhibit Growth of Phytium sp. In Vitro. The study aimed at testing the potential of some antagonistic fungi isolated from durian seedlings media to inhibit growth of Phytium sp. Research was done at the Central Laboratory of Tropical Fruit Research Solok in July-September 2009 by using a complete randomized design with 5 treatments and 4 replications. Tests was conducted by dual culture method on PDA. The results showed that Gliocladium sp., Trichoderma sp.a, Trichoderma sp.b, Aspergilus sp., and Penicillium sp. can inhibit growth of Phytium sp., with growth inhibition of 50, 49.5, 47, 48, and 38.3% respectively. Inhibition mecanism of Gliocladium sp., and Trichoderma sp. were competition, antibiosis, lisis, and parasitism, while Penicillium sp. was antibiosis. Gliocladium sp., Trichoderma sp.a, Trichoderma sp.b, Aspergilus sp., and Penicillium sp. can be used as biological agents to control pathogenic fungi Phytium sp.</p><p> </p><p><strong>Abstrak</strong></p><p>Penelitian bertujuan untuk menguji potensi beberapa cendawan antagonis hasil isolasi dari media pembibitan durian dalam menghambat pertumbuhan Phytium sp. Penelitian dilakukan di Laboratorium Balai Penelitian Tanaman Buah Tropika Solok pada bulan Juli-September 2010. Penelitian disusun dalam rancangan acak lengkap dengan 5 perlakuan dan 4 ulangan. Pengujian daya antagonis cendawan dilakukan dengan metode dual culture yang diinokulasikan pada media PDA. Hasil penelitian menunjukkan bahwa Gliocladium sp., Trichoderma sp.a, Trichoderma sp.b., Aspergilus sp., dan Penicillium sp. dapat menghambat pertumbuhan Phytium sp. secara in vitro, dengan daya hambat masing-masing 50; 49,5; 47; 48; dan 38,3% secara berurutan. Mekanisme antagonis Gliocladium sp. dan Trichoderma sp. adalah kompetisi, antibiosis, lisis, dan parasitisme, sedangkan Penicillium sp. hanya bersifat antibiosis. Gliocladium sp., Trichoderma sp., Aspergilus sp., dan Penicillium sp. dapat digunakan sebagai agen hayati untuk mengendalikan cendawan patogen Phytium sp.</p>


2018 ◽  
Vol 10 (9) ◽  
pp. 344
Author(s):  
Caroline de Araujo Machado ◽  
Leila Albuquerque Resende de Oliveira ◽  
Annie Carolina Araújo de Oliveira ◽  
Milena Nascimento Cardoso ◽  
Francine Ferreira Padilha ◽  
...  

Mangaba tree (Hancornia speciosa Gomes) is a tropical fruit species from Brazil and presents socioeconomic potential. The objective of this study was to determine antioxidant activity, rutin content and genetic similarity among in vivo and in vitro matrices and progenies of six accessions from the Mangaba Active Germplasm Bank of the Embrapa Coastal Tablelands, SE, Brazil. Young leaves of adult matrix plants and in vitro callus methanolic extracts obtained from young leaves resulted in differences between the accessions for the rutin content and high antioxidant activity. The Costa Azul accession, from Bahia, Brazil outstood with values above 3,000 g of dry extract/g of DPPH (2.2-diphenyl-1-picrilhydrazyl) in in vitro callus coming from nodal and internodal segments (3,023.73 and 3,136.80 g of dry extract/g of DPPH, respectively). Rutin was not found in extracts resulting from in vitro callus of mangaba tree. The difference among the values obtained for DPPH analysis are superior when compared with in vivo leaf extract, with the in vitro Costa Azul accession (nodal and internodal) outstanding, concluding that in vitro callus induction can interfere with chemical compounds of the plant. The markers detect genetic similarity under in vitro cultivation conditions. The Costa Azul accession group itself in isolation from others and EC50 concentrations differentiated between leaf and callus are obtained. O EC50 is superior in extracts deriving from in vitro callus, with the in vitro Costa Azul accession (nodal and internodal).


2014 ◽  
Vol 4 ◽  
pp. S665-S671 ◽  
Author(s):  
Priti Gupta ◽  
Ira Bhatnagar ◽  
Se-Kwon Kim ◽  
Ajay Kumar Verma ◽  
Anubhuti Sharma

1962 ◽  
Vol 40 (9) ◽  
pp. 1195-1201 ◽  
Author(s):  
S. J. Patrick

Hypoglycin A, an amino acid of novel structure isolated from a tropical fruit, Blighia sapida, is known to cause a profound hypoglycaemia when administered to animals. The in vitro effects of hypoglycin A on the metabolism of glucose C14by normal rat liver slices, rat diaphragm, and rat adipose tissue have been investigated. These experiments have been carried out in the absence and in the presence of insulin. With liver, hypoglycin A decreased the conversion of glucose C14to glycogen and fatty acid but increased the conversion to pyruvate. With diaphragms, hypoglycin A increased conversion of glucose C14to glycogen and carbon dioxide but only in the absence of insulin. No changes in glucose C14metabolism by the epididymal fat pad were demonstrated as a result of the presence of hypoglycin. Theories on the mechanism of action of hypoglycin A are discussed.


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