A novel subtilin-like lantibiotics subtilin JS-4 produced by Bacillus subtilis JS-4, and its antibacterial mechanism against Listeria monocytogenes

ATP-binding cassette (ABC) transporters are usually involved in the translocation of their cognate substrates, which is driven by ATP hydrolysis. Typically, these transporters are required for the import or export of a wide range of substrates such as sugars, ions and complex organic molecules. ABC exporters can also be involved in the export of toxic compounds such as antibiotics. However, recent studies revealed alternative detoxification mechanisms of ABC transporters. For instance, the ABC transporter BceAB of Bacillus subtilis seems to confer resistance to bacitracin via target protection. In addition, several transporters with functions other than substrate export or import have been identified in the past. Here, we provide an overview of recent findings on ABC transporters of the Gram-positive organisms B. subtilis and Listeria monocytogenes with transport or regulatory functions affecting antibiotic resistance, cell wall biosynthesis, cell division and sporulation.

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Transcriptional activation of the Listeria monocytogenes hemolysin gene in Bacillus subtilis.

Journal of Bacteriology ◽

10.1128/jb.174.4.1293-1298.1992 ◽

1992 ◽

Vol 174 (4) ◽

pp. 1293-1298 ◽

Cited By ~ 45

Author(s):

N E Freitag ◽

P Youngman ◽

D A Portnoy

Keyword(s):

Bacillus Subtilis ◽

Listeria Monocytogenes ◽

Transcriptional Activation ◽

Hemolysin Gene

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Antibacterial mechanism of ε -Poly-lysine against Listeria monocytogenes and its application on cheese

Food Control ◽

10.1016/j.foodcont.2018.03.025 ◽

2018 ◽

Vol 91 ◽

pp. 76-84 ◽

Cited By ~ 50

Author(s):

Lin Lin ◽

Yulei Gu ◽

Changzhu Li ◽

Saritporn Vittayapadung ◽

Haiying Cui

Keyword(s):

Listeria Monocytogenes ◽

Antibacterial Mechanism

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Antimicrobial Susceptibility and Antibacterial Mechanism of Limonene against Listeria monocytogenes

Molecules ◽

10.3390/molecules25010033 ◽

2019 ◽

Vol 25 (1) ◽

pp. 33 ◽

Cited By ~ 6

Author(s):

Yingjie Han ◽

Zhichang Sun ◽

Wenxue Chen

Keyword(s):

Listeria Monocytogenes ◽

Cell Membrane ◽

Respiratory Chain ◽

Antimicrobial Susceptibility ◽

Chain Complex ◽

Cell Membrane Permeability ◽

Wall Structure ◽

Antibacterial Mechanism ◽

Respiratory Chain Complex ◽

Complex Activity

Limonene is a monoterpenoid compound, which is founded in a lot of plants’ essential oils with good antibacterial activity against food-borne pathogens, but it has an ambiguous antimicrobial susceptibility and mechanism against Listeria monocytogenes (L. monocytogenes). In this study, the antimicrobial susceptibility of Limonene to L. monocytogenes was studied, and some new sights regarding its antibacterial mechanism were further explored. Scanning electron microscopy (SEM) verified that limonene caused the destruction of the cell integrity and wall structure of L. monocytogenes. The increase in conductivity and the leakage of intracellular biomacromolecules (nucleic acids and proteins) confirmed that limonene had an obvious effect on cell membrane permeability. The results of Propidium Iodide (PI) fluorescence staining were consistent with the results of the conductivity measurements. This indicated that limonene treatment caused damage to the L. monocytogenes cell membrane. Furthermore, the decrease in ATP content, ATPase (Na+K+-ATPase, Ca2+-ATPase) activity and respiratory chain complex activity indicated that limonene could hinder ATP synthesis by inhibiting the activity of the respiratory complex and ATPase. Finally, differential expression of proteins in the respiratory chain confirmed that limonene affected respiration and energy metabolism by inhibiting the function of the respiratory chain complex.

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