Allyl Isothiocyanate Release from Edible Laminaria japonica for Time-Dependent Growth Deactivation of Foodborne Pathogens: I: Micrococcus luteus, Bacillus subtilis, and Listeria monocytogenes

ATP-binding cassette (ABC) transporters are usually involved in the translocation of their cognate substrates, which is driven by ATP hydrolysis. Typically, these transporters are required for the import or export of a wide range of substrates such as sugars, ions and complex organic molecules. ABC exporters can also be involved in the export of toxic compounds such as antibiotics. However, recent studies revealed alternative detoxification mechanisms of ABC transporters. For instance, the ABC transporter BceAB of Bacillus subtilis seems to confer resistance to bacitracin via target protection. In addition, several transporters with functions other than substrate export or import have been identified in the past. Here, we provide an overview of recent findings on ABC transporters of the Gram-positive organisms B. subtilis and Listeria monocytogenes with transport or regulatory functions affecting antibiotic resistance, cell wall biosynthesis, cell division and sporulation.

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Experimental observation of viscoelastic fluid–structure interactions

Journal of Fluid Mechanics ◽

10.1017/jfm.2017.15 ◽

2017 ◽

Vol 813 ◽

Cited By ~ 13

Author(s):

Anita A. Dey ◽

Yahya Modarres-Sadeghi ◽

Jonathan P. Rothstein

Keyword(s):

Flow Instability ◽

Reynolds Numbers ◽

Time Dependent ◽

Flexible Structure ◽

Fluid Inertia ◽

Fluid Structure Interactions ◽

Periodic Oscillations ◽

Fluid Structure ◽

Dependent Growth ◽

First Time

It is well known that when a flexible or flexibly mounted structure is placed perpendicular to the flow of a Newtonian fluid, it can oscillate due to the shedding of separated vortices. Here, we show for the first time that fluid–structure interactions can also be observed when the fluid is viscoelastic. For viscoelastic fluids, a flexible structure can become unstable in the absence of fluid inertia, at infinitesimal Reynolds numbers, due to the onset of a purely elastic flow instability. Nonlinear periodic oscillations of the flexible structure are observed and found to be coupled to the time-dependent growth and decay of viscoelastic stresses in the wake of the structure.

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A novel subtilin-like lantibiotics subtilin JS-4 produced by Bacillus subtilis JS-4, and its antibacterial mechanism against Listeria monocytogenes

LWT ◽

10.1016/j.lwt.2021.110993 ◽

2021 ◽

Vol 142 ◽

pp. 110993

Author(s):

Zhaohui Wei ◽

Chengjun Shan ◽

Lixia Zhang ◽

Da'e Ge ◽

Ying Wang ◽

...

Keyword(s):

Bacillus Subtilis ◽

Listeria Monocytogenes ◽

Antibacterial Mechanism

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Changes in Aerobic Plate and Escherichia coli–Coliform Counts and in Populations of Inoculated Foodborne Pathogens on Inshell Walnuts during Storage

Journal of Food Protection ◽

10.4315/0362-028x.jfp-15-553 ◽

2016 ◽

Vol 79 (7) ◽

pp. 1143-1153 ◽

Cited By ~ 8

Author(s):

JOHN C. FRELKA ◽

GORDON R. DAVIDSON ◽

LINDA J. HARRIS

Keyword(s):

Escherichia Coli ◽

Listeria Monocytogenes ◽

Relative Humidity ◽

Low Temperature ◽

Foodborne Pathogens ◽

Limit Of Detection ◽

Sampling Time ◽

E Coli ◽

Plate Counts ◽

Forced Air

ABSTRACT After harvest, inshell walnuts are dried using low-temperature forced air and are then stored in bins or silos for up to 1 year. To better understand the survival of bacteria on inshell walnuts, aerobic plate counts (APCs) and Escherichia coli–coliform counts (ECCs) were evaluated during commercial storage (10 to 12°C and 63 to 65% relative humidity) over 9 months. APCs decreased by 1.4 to 2.0 log CFU per nut during the first 5 months of storage, and ECCs decreased by 1.3 to 2.2 log CFU per nut in the first month of storage. Through the remaining 4 to 8 months of storage, APCs and ECCs remained unchanged (P > 0.05) or decreased by <0.15 log CFU per nut per month. Similar trends were observed on kernels extracted from the inshell walnuts. APCs and ECCs were consistently and often significantly higher on kernels extracted from visibly broken inshell walnuts than on kernels extracted from visibly intact inshell walnuts. Parameters measured in this study were used to determine the survival of five-strain cocktails of E. coli O157:H7, Listeria monocytogenes, and Salmonella inoculated onto freshly hulled inshell walnuts (~8 log CFU/g) after simulated commercial drying (10 to 12 h; 40°C) and simulated commercial storage (12 months at 10°C and 65% relative humidity). Populations declined by 2.86, 5.01, and 4.40 log CFU per nut for E. coli O157:H7, L. monocytogenes, and Salmonella, respectively, after drying and during the first 8 days of storage. Salmonella populations changed at a rate of −0.33 log CFU per nut per month between days 8 and 360, to final levels of 2.83 ± 0.79 log CFU per nut. E. coli and L. monocytogenes populations changed by −0.17 log CFU per nut per month and −0.26 log CFU per nut per month between days 8 and 360, respectively. For some samples, E. coli or L. monocytogenes populations were below the limit of detection by plating (0.60 log CFU per nut) by day 183 or 148, respectively; at least one of the six samples was positive at each subsequent sampling time by either plating or by enrichment.

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Evaluation of Gaseous Chlorine Dioxide as a Sanitizer for Killing Salmonella, Escherichia coli O157:H7, Listeria monocytogenes, and Yeasts and Molds on Fresh and Fresh-Cut Produce

Journal of Food Protection ◽

10.4315/0362-028x-68.6.1176 ◽

2005 ◽

Vol 68 (6) ◽

pp. 1176-1187 ◽

Cited By ~ 112

Author(s):

KAYE V. SY ◽

MELINDA B. MURRAY ◽

M. DAVID HARRISON ◽

LARRY R. BEUCHAT

Keyword(s):

Escherichia Coli ◽

Listeria Monocytogenes ◽

Chlorine Dioxide ◽

Foodborne Pathogens ◽

Escherichia Coli O157 ◽

Gaseous Chlorine Dioxide ◽

Sensory Qualities ◽

A Cell ◽

Fresh Cut ◽

Yeasts And Molds

Gaseous chlorine dioxide (ClO2) was evaluated for effectiveness in killing Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes on fresh-cut lettuce, cabbage, and carrot and Salmonella, yeasts, and molds on apples, peaches, tomatoes, and onions. Inoculum (100 μl, ca. 6.8 log CFU) containing five serotypes of Salmonella enterica, five strains of E. coli O157:H7, or five strains of L. monocytogenes was deposited on the skin and cut surfaces of fresh-cut vegetables, dried for 30 min at 22°C, held for 20 h at 4°C, and then incubated for 30 min at 22°C before treatment. The skin surfaces of apples, peaches, tomatoes, and onions were inoculated with 100 μl of a cell suspension (ca. 8.0 log CFU) containing five serotypes of Salmonella, and inoculated produce was allowed to dry for 20 to 22 h at 22°C before treatment. Treatment with ClO2 at 4.1 mg/liter significantly (α = 0.05) reduced the population of foodborne pathogens on all produce. Reductions resulting from this treatment were 3.13 to 4.42 log CFU/g for fresh-cut cabbage, 5.15 to 5.88 log CFU/g for fresh-cut carrots, 1.53 to 1.58 log CFU/g for fresh-cut lettuce, 4.21 log CFU per apple, 4.33 log CFU per tomato, 1.94 log CFU per onion, and 3.23 log CFU per peach. The highest reductions in yeast and mold populations resulting from the same treatment were 1.68 log CFU per apple and 2.65 log CFU per peach. Populations of yeasts and molds on tomatoes and onions were not significantly reduced by treatment with 4.1 mg/liter ClO2. Substantial reductions in populations of pathogens on apples, tomatoes, and onions but not peaches or fresh-cut cabbage, carrot, and lettuce were achieved by treatment with gaseous ClO2 without markedly adverse effects on sensory qualities.

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Time-dependent growth of crystalline Au0-nanoparticles in cyanobacteria as self-reproducing bioreactors: 2.Anabaena cylindrica

Beilstein Journal of Nanotechnology ◽

10.3762/bjnano.7.30 ◽

2016 ◽

Vol 7 ◽

pp. 312-327 ◽

Cited By ~ 13

Author(s):

Liz M Rösken ◽

Felix Cappel ◽

Susanne Körsten ◽

Christian B Fischer ◽

Andreas Schönleber ◽

...

Keyword(s):

Extracellular Polymeric Substances ◽

Production Method ◽

Wide Distribution ◽

Time Dependent ◽

Anabaena Cylindrica ◽

Vegetative Cells ◽

Breakdown Spectroscopy ◽

Laser Induced Breakdown ◽

Dependent Growth ◽

Average Size

Microbial biosynthesis of metal nanoparticles as needed in catalysis has shown its theoretical ability as an extremely environmentally friendly production method in the last few years, even though the separation of the nanoparticles is challenging. Biosynthesis, summing up biosorption and bioreduction of diluted metal ions to zero valent metals, is especially ecofriendly, when the bioreactor itself is harmless and needs no further harmful reagents. The cyanobacteriumAnabaena cylindrica(SAG 1403.2) is able to form crystalline Au0-nanoparticles from Au3+ions and does not release toxic anatoxin-a. X-ray powder diffraction (XRD), transmission electron microscopy (TEM) and laser-induced breakdown spectroscopy (LIBS) are applied to monitor the time-dependent development of gold nanoparticles for up to 40 hours. Some vegetative cells (VC) are filled with nanoparticles within minutes, while the extracellular polymeric substances (EPS) of vegetative cells and the heterocyst polysaccharide layer (HEP) are the regions, where the first nanoparticles are detected on most other cells. The uptake of gold starts immediately after incubation and within four hours the average size remains constant around 10 nm. Analyzing the TEM images with an image processing program reveals a wide distribution for the diameter of the nanoparticles at all times and in all regions of the cyanobacteria. Finally, the nanoparticle concentration in vegetative cells ofAnabaena cylindricais about 50% higher than in heterocysts (HC). These nanoparticles are found to be located along the thylakoid membranes.

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Survival of foodborne pathogens ( Escherichia coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus , Listeria monocytogenes , and Vibrio parahaemolyticus ) in raw ready-to-eat crab marinated in soy sauce

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2016.08.041 ◽

2016 ◽

Vol 238 ◽

pp. 50-55 ◽

Cited By ~ 22

Author(s):

T.J. Cho ◽

N.H. Kim ◽

S.A. Kim ◽

J.H. Song ◽

M.S. Rhee

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Listeria Monocytogenes ◽

Salmonella Typhimurium ◽

Foodborne Pathogens ◽

Vibrio Parahaemolyticus ◽

Soy Sauce ◽

Escherichia Coli O157

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Global Proteomic Analysis of Listeria monocytogenes’ Response to Linalool

Foods ◽

10.3390/foods10102449 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2449

Author(s):

Zhipeng Gao ◽

Weiming Zhong ◽

Ting Liu ◽

Tianyu Zhao ◽

Jiajing Guo

Keyword(s):

Listeria Monocytogenes ◽

Foodborne Pathogens ◽

Proteomic Analysis ◽

Drug Targets ◽

Enrichment Analysis ◽

Good Evidence ◽

New Drugs ◽

Organic System ◽

Flow Cytometry Data ◽

Theoretical Support

Listeria monocytogenes (LM) is one of the most serious foodborne pathogens. Listeriosis, the disease caused by LM infection, has drawn attention worldwide because of its high hospitalization and mortality rates. Linalool is a vital constituent found in many essential oils; our previous studies have proved that linalool exhibits strong anti-Listeria activity. In this study, iTRAQ-based quantitative proteomics analysis was performed to explore the response of LM exposed to linalool, and to unravel the mode of action and drug targets of linalool against LM. A total of 445 differentially expressed proteins (DEPs) were screened out, including 211 up-regulated and 234 down-regulated proteins which participated in different biological functions and pathways. Thirty-one significantly enriched gene ontology (GO) functional categories were obtained, including 12 categories in “Biological Process”, 10 categories in “Cell Component”, and 9 categories in “Molecular Function”. Sixty significantly enriched biological pathways were classified, including 6 pathways in “Cell Process”, 6 pathways in “Environmental Information Processing”, 3 pathways in “Human Disease”, 40 pathways in “Metabolism”, and 2 pathways in “Organic System”. GO and Kyoto Encyclopedia of Genes (KEGG) enrichment analysis together with flow cytometry data implied that cell membranes, cell walls, nucleoids, and ribosomes might be the targets of linalool against LM. Our study provides good evidence for the proteomic analysis of bacteria, especially LM, exposed to antibacterial agents. Further, those drug targets discovered by proteomic analysis can provide theoretical support for the development of new drugs against LM.

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Effect of Oxygen Stress on Growth and Survival of Clostridium perfringens, Campylobacter jejuni, and Listeria monocytogenes under Different Storage Conditions

Journal of Food Protection ◽

10.4315/0362-028x.jfp-14-427 ◽

2015 ◽

Vol 78 (4) ◽

pp. 691-697 ◽

Cited By ~ 13

Author(s):

HAMZAH AL-QADIRI ◽

SHYAM S. SABLANI ◽

MAHMOUDREZA OVISSIPOUR ◽

NIVIN AL-ALAMI ◽

BYJU GOVINDAN ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Campylobacter Jejuni ◽

Clostridium Perfringens ◽

Foodborne Pathogens ◽

Storage Conditions ◽

Growth And Survival ◽

Anoxic Conditions ◽

Initial Inoculum ◽

Log Reduction ◽

Packaged Food

This study investigated the growth and survival of three foodborne pathogens (Clostridium perfringens, Campylobacter jejuni, and Listeria monocytogenes) in beef (7% fat) and nutrient broth under different oxygen levels. Samples were tested under anoxic (<0.5%), microoxic (6 to 8%), and oxic (20%) conditions during storage at 7°C for 14 days and at 22°C for 5 days. Two initial inoculum concentrations were used (1 and 2 log CFU per g of beef or per ml of broth). The results show that C. perfringens could grow in beef at 22°C, with an increase of approximately 5 log under anoxic conditions and a 1-log increase under microoxic conditions. However, C. perfringens could not survive in beef held at 7°C under microoxic and oxic storage conditions after 14 days. In an anoxic environment, C. perfringens survived in beef samples held at 7°C, with a 1-log reduction. A cell decline was observed at 2 log under these conditions, with no surviving cells at the 1-log level. However, the results show that C. jejuni under microoxic conditions survived with declining cell numbers. Significant increases in L. monocytogenes (5 to 7 log) were observed in beef held at 22°C for 5 days, with the lowest levels recovered under anoxic conditions. L. monocytogenes in refrigerated storage increased by a factor of 2 to 4 log. It showed the greatest growth under oxic conditions, with significant growth under anoxic conditions. These findings can be used to enhance food safety in vacuum-packed and modified atmosphere–packaged food products.

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