The effect of Ditylum brightwellii (Bacillariophyceae) on colony development of bloom forming species Phaeocystis globosa (Prymnesiophyceae) under nutrient-replete condition

2021 ◽  
Vol 167 ◽  
pp. 112336
Author(s):  
Qi Liu ◽  
Rui-Juan Zhang ◽  
Lu Huang ◽  
Jia-Wei Zhang ◽  
Su-Qin Zhuo ◽  
...  
Keyword(s):  
Colony Development ◽  
Phaeocystis Globosa ◽  
Ditylum Brightwellii

scholarly journals Bacterial Composition Associated With Giant Colonies of the Harmful Algal Species Phaeocystis globosa

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhu Zhu ◽  
Rui Meng ◽  
Walker O. Smith Jr. ◽  
Hai Doan-Nhu ◽  
Lam Nguyen-Ngoc ◽  
...  
Keyword(s):  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Algal Species ◽  
Microbial Consortia ◽  
Colony Development ◽  
Microbial Composition ◽  
Phaeocystis Globosa ◽  
Ambient Seawater ◽  
Bacterial Composition ◽  
Bacterial Consortia

The cosmopolitan algae Phaeocystis globosa forms harmful algal blooms frequently in a number of tropical and subtropical coastal regions in the past two decades. During the bloom, the giant colony, which is formed by P. globosa, is the dominant morphotype. However, the microenvironment and the microbial composition in the intracolonial fluid are poorly understood. Here, we used high-throughput 16S rRNA amplicon sequencing to examine the bacterial composition and predicted functions in intracolonial fluid. Compared with the bacterial consortia in ambient seawater, intracolonial fluids possessed the lower levels of microbial richness and diversity, implying selectivity of bacteria by the unique intracolonial microenvironment enclosed within the P. globosa polysaccharide envelope. The bacterial consortia in intracolonial fluid were dominated by Balneola (48.6% of total abundance) and Labrezia (28.5%). The bacteria and microbial function enriched in intracolonial fluid were involved in aromatic benzenoid compounds degradation, DMSP and DMS production and consumption, and antibacterial compounds synthesis. We suggest that the P. globosa colonial envelope allows for the formation of a specific microenvironment; thus, the unique microbial consortia inhabiting intracolonial fluid has close interaction with P. globosa cells, which may benefit colony development.

scholarly journals Effects of Modified Clay on Phaeocystis globosa Growth and Colony Formation

2021 ◽  
Vol 18 (19) ◽  
pp. 10163
Author(s):  
Xiangzheng Ren ◽  
Zhiming Yu ◽  
Lixia Qiu ◽  
Xihua Cao ◽  
Xiuxian Song
Keyword(s):  
Colony Formation ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Cell Damage ◽  
Extracellular Polysaccharide ◽  
Inorganic Phosphorus ◽  
Colony Development ◽  
Stress Concentrations ◽  
Phaeocystis Globosa ◽  
Modified Clay

Phaeocystis globosa is a globally distributed harmful algal blooms (HABs) species dominated by the colonial morphotype, which presents dramatic environmental hazards and poses a threat to human health. Modified clay (MC) can effectively flocculate HAB organisms and prevent their subsequent growth, but the effects of MC on colony-dominated P. globosa blooms remain uncertain. In this paper, a series of removal and incubation experiments were conducted to investigate the growth, colony formation and colony development of P. globosa cells after treatment with MC. The results show that the density of colonies was higher at MC concentrations below 0.2 g/L compared to those in the control, indicating the role of P. globosa colonies in resistance to environmental stress. Concentrations of MC greater than 0.2 g/L could reduce the density of solitary cells and colonies, and the colony diameter and extracellular polysaccharide (EPS) content were also decreased. The adsorption of MC to dissolved inorganic phosphorus (DIP) and the cell damage caused by collision may be the main mechanisms underlying this phenomenon. These results elucidate that the treatment with an appropriate concentration of MC may provide an effective mitigation strategy for P. globosa blooms by preventing their growth and colony formation.

Differential fiu-lacZ fusion regulation linked to Escherichia coli colony development

1999 ◽  
Vol 33 (1) ◽  
pp. 18-32 ◽  
Author(s):  
Dina L. Newman ◽  
James A. Shapiro
Keyword(s):  
Escherichia Coli ◽  
Lacz Fusion ◽  
Colony Development

Toxicity assessment of chronic exposure to common insecticides and bee medications on colony development and drones sperm parameters

Ecotoxicology ◽  
2021 ◽  
Author(s):  
Faten Ben Abdelkader ◽  
İbrahim Çakmak ◽  
Selvinar Seven Çakmak ◽  
Zekariya Nur ◽  
Ece İncebıyık ◽  
...  
Keyword(s):  
Chronic Exposure ◽  
Toxicity Assessment ◽  
Sperm Parameters ◽  
Colony Development

scholarly journals Mitochondrial Retrograde Signaling Contributes to Metabolic Differentiation in Yeast Colonies

2021 ◽  
Vol 22 (11) ◽  
pp. 5597
Author(s):  
Vítězslav Plocek ◽  
Kristýna Fadrhonc ◽  
Jana Maršíková ◽  
Libuše Váchová ◽  
Alexandra Pokorná ◽  
...  
Keyword(s):  
Cell Types ◽  
Colony Development ◽  
Mitochondrial Activity ◽  
Mitochondrial Translation ◽  
Metabolic Intermediates ◽  
Nutritional Conditions ◽  
Mitochondrial Ribosome ◽  
Development And Differentiation ◽  
Cell Subpopulations ◽  
Different Cell Types

During development of yeast colonies, various cell subpopulations form, which differ in their properties and specifically localize within the structure. Three branches of mitochondrial retrograde (RTG) signaling play a role in colony development and differentiation, each of them activating the production of specific markers in different cell types. Here, aiming to identify proteins and processes controlled by the RTG pathway, we analyzed proteomes of individual cell subpopulations from colonies of strains, mutated in genes of the RTG pathway. Resulting data, along with microscopic analyses revealed that the RTG pathway predominantly regulates processes in U cells, long-lived cells with unique properties, which are localized in upper colony regions. Rtg proteins therein activate processes leading to amino acid biosynthesis, including transport of metabolic intermediates between compartments, but also repress expression of mitochondrial ribosome components, thus possibly contributing to reduced mitochondrial translation in U cells. The results reveal the RTG pathway’s role in activating metabolic processes, important in U cell adaptation to altered nutritional conditions. They also point to the important role of Rtg regulators in repressing mitochondrial activity in U cells.

scholarly journals Isolation and Phylogenetic Analysis of Novel Viruses Infecting the Phytoplankton Phaeocystis globosa (Prymnesiophyceae)

2004 ◽  
Vol 70 (6) ◽  
pp. 3700-3705 ◽  
Author(s):  
C. P. D. Brussaard ◽  
S. M. Short ◽  
C. M. Frederickson ◽  
C. A. Suttle
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Polymerase ◽  
Coastal Waters ◽  
Common Ancestor ◽  
Genetic Relatedness ◽  
Amino Acid Sequences ◽  
Polymerase Gene ◽  
Phaeocystis Globosa ◽  
The Family ◽  
Algal Host

ABSTRACT Viruses infecting the harmful bloom-causing alga Phaeocystis globosa (Prymnesiophyceae) were readily isolated from Dutch coastal waters (southern North Sea) in 2000 and 2001. Our data show a large increase in the abundance of putative P. globosa viruses during blooms of P. globosa, suggesting that viruses are an important source of mortality for this alga. In order to examine genetic relatedness among viruses infecting P. globosa and other phytoplankton, DNA polymerase gene (pol) fragments were amplified and the inferred amino acid sequences were phylogenetically analyzed. The results demonstrated that viruses infecting P. globosa formed a closely related monophyletic group within the family Phycodnaviridae, with at least 96.9% similarity to each other. The sequences grouped most closely with others from viruses that infect the prymnesiophyte algae Chrysochromulina brevifilum and Chrysochromulina strobilus. Whether the P. globosa viruses belong to the genus Prymnesiovirus or form a separate group needs further study. Our data suggest that, like their phytoplankton hosts, the Chrysochromulina and Phaeocystis viruses share a common ancestor and that these prymnesioviruses and their algal host have coevolved.

scholarly journals Menaquinone and Iron Are Essential for Complex Colony Development in Bacillus subtilis

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e79488 ◽  
Author(s):  
Gidi Pelchovich ◽  
Shira Omer-Bendori ◽  
Uri Gophna
Keyword(s):  
Bacillus Subtilis ◽  
Colony Development

scholarly journals Investigation of the Inhibitory Effects of Mangrove Leaves and Analysis of Their Active Components on Phaeocystis globosa during Different Stages of Leaf Age

2018 ◽  
Vol 15 (11) ◽  
pp. 2434 ◽  
Author(s):  
Min Zhao ◽  
Han Xiao ◽  
Dong Sun ◽  
Shunshan Duan
Keyword(s):  
Secondary Metabolites ◽  
Harmful Algae ◽  
Marine Ecology ◽  
Phenolic Glycoside ◽  
Aqueous Extracts ◽  
Red Tides ◽  
Inhibitory Effects ◽  
Phaeocystis Globosa ◽  
Biological Remediation ◽  
Young Leaves

The presence of harmful algal blooms (HABs) can cause significant problems to the quality of the water, the marine ecosystems, and the human health, and economy worldwide. Biological remediation can inhibit harmful algal growth efficiently in an environmental-friendly manner. Therefore, the research conducted on biological remediation with regard to the inhibition of HABs is becoming a major focus in marine ecology. To date, no study has been reported with regard to the red tides occurring in mangrove wetlands. Therefore, the present study used two mangrove species, namely Bruguiera gymnorrhiza and Kandelia candel and one harmful algae species Phaeocystis globosa as experimental organisms. The present study determined the inhibitory effects and algae physiology of specific aqueous extracts from mangrove leaves on the viability of harmful algae, and analyzed the main chemical composition of the aqueous extracts by ultra-performance liquid chromatography coupled to high resolution mass spectrometry (UPLC-QTOF-MS). The results indicated that the aqueous extracts from different leaf ages of B. gymnorrhiza and K. candel leaves exhibited apparent inhibitory effects on the growth of P. globosa. The inhibitory effects of B. gymnorrhiza and K. candel leaves aqueous extracts on the growth of P. globosa were in the following order: senescent > mature > young leaves. The levels of the parameters superoxide dismutase (SOD) activity, glutathione (GSH), and malondialdehyde (MDA)content in P. globosa following treatment with B. gymnorrhiza and K. candel leaves aqueous extracts were increased as follows: senescent > mature > young leaves. Simultaneously, the intensity of the ion peaks of the specific secondary metabolites assigned 4 (No.: 4 Rt: 2.83 min), 7 (No.: 7 Rt: 3.14 min), 8 (No.: 8 Rt: 3.24 min), 9 (No.: 9 Rt: 3.82min) and 10 (No.: 10 Rt: 4.10 min) were increased. These metabolites were found in the aqueous extracts from B. gymnorrhiza leaves. The intensities of the ion peaks of the secondary metabolites 7, 8 in the aqueous extracts from the K. candel leaves were also increased. The majority of the substances that inhibited the algae found in the mangrove plants were secondary metabolites. Therefore, we considered that the norsesquiterpenes compounds 4, 8, 9, and 10 and a phenolic glycoside compound 7 were the active constituents in the aqueous extracts of the mangrove leaves responsible for the inhibition of algae growth. This evidence provided theoretical guidance for the development of biological methods to control red tides and for the further use of substances with antiproliferative activity against algae.

The adaptation of Bact. coli mutabile to lactose

1954 ◽  
Vol 223 (1154) ◽  
pp. 420-420
Keyword(s):  
Inoculum Size ◽  
Colony Development ◽  
Lag Phase ◽  
Normal Cells ◽  
Adaptive Process ◽  
Large Numbers ◽  
Parent Culture ◽  
Mutation Theory ◽  
Special Circumstances ◽  
New Evidence

When Bact. coli mutabile not previously exposed to lactose is plated on lactose-ammonium sulphate agar the number of normal-sized colonies (lac + ) eventually formed is a complicated function of the inoculum size. For small numbers all the cells plated eventually form colonies; for large numbers the colony yield is determined not by a number of mutants in the parent culture but by plate exhaustion (for which the earlier developing colonies are chiefly responsible). The time of appearance of the lac + colonies is much longer than with a culture previously grown in lactose. Thus lac + mutants could not have been present from the start unless their growth is inhibited by an excess of normal cells. When, however, a small number of previously adapted cells are mixed with an excess of unadapted cells the presence of the latter does not impede the development on agar of lac + colonies from the former. When cells are first placed in a liquid lactose medium and samples are transferred at intervals during the ensuing lag phase, the time needed for colony development on lactose-agar progressively diminishes, once again showing that an adaptive process is occurring during the lag in the liquid medium. In certain special circumstances the adaptation to the liquid lactose medium may occur with abnormal speed. The growth rate of newly adapted strains is at first variable. If interpreted by a mutation theory the observations would demand the assumption of a complex polygenetic system for which current applications of the Luria-Delbrück and Lea-Coulson theories would be invalid. Recent arguments about the mutational nature of these phenomena are criticized in the light of the new evidence.

scholarly journals Phylogenetic Responses of Marine Free-Living Bacterial Community to Phaeocystis globosa Bloom in Beibu Gulf, China

2020 ◽  
Vol 11 ◽  
Author(s):  
Nan Li ◽  
Huaxian Zhao ◽  
Gonglingxia Jiang ◽  
Qiangsheng Xu ◽  
Jinli Tang ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Beibu Gulf ◽  
Free Living ◽  
Phaeocystis Globosa
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