Fabrication, characterization and in vitro cell study of gelatin-chitosan scaffolds: New perspectives of use of aloe vera and snail mucus for soft tissue engineering

A electrospinning process to prepare soft tissue engineering scaffold was introduced in this study. This kind of scaffold was composed with ultrathin fiber and characterized with high porosity, well-interconnected pores and high surface-to-volume ratio. Biodegradable polylaticacid (PLA) was used to spin the scaffold and the scaffold was evaluated in vitro by analysis the microscopic structure, porosity, mechanical property, especially cytocompatibility. The results indicated that the electrospun PLA scaffold showed good cytocompatibility and the tensile property of electrospun scaffold was similar to human’s soft tissue. It could be expected that the electrospun scaffold would be potential in soft tissue engineering or soft tissue repair.

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Fiber-Based chitosan tubular scaffolds for soft tissue engineering: Fabrication and in vitro evaluation

Tsinghua Science & Technology ◽

10.1016/s1007-0214(05)70099-7 ◽

2005 ◽

Vol 10 (4) ◽

pp. 449-453 ◽

Cited By ~ 13

Author(s):

Aijun Wang ◽

Qiang Ao ◽

Wenling Cao ◽

Chang Zhao ◽

Yandao Gong ◽

...

Keyword(s):

Tissue Engineering ◽

Soft Tissue ◽

In Vitro Evaluation ◽

Soft Tissue Engineering

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Thermal-crosslinked porous chitosan scaffolds for soft tissue engineering applications

Materials Science and Engineering C ◽

10.1016/j.msec.2013.05.010 ◽

2013 ◽

Vol 33 (7) ◽

pp. 3780-3785 ◽

Cited By ~ 20

Author(s):

Chengdong Ji ◽

Jeffrey Shi

Keyword(s):

Tissue Engineering ◽

Soft Tissue ◽

Engineering Applications ◽

Porous Chitosan ◽

Chitosan Scaffolds ◽

Soft Tissue Engineering

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Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineering

Journal of Tissue Engineering ◽

10.1177/2041731417744157 ◽

2017 ◽

Vol 8 ◽

pp. 204173141774415 ◽

Cited By ~ 14

Author(s):

Lukas Kessler ◽

Sandra Gehrke ◽

Marc Winnefeld ◽

Birgit Huber ◽

Eva Hoch ◽

...

Keyword(s):

Tissue Engineering ◽

Soft Tissue ◽

Adipogenic Differentiation ◽

Building Blocks ◽

Fatty Acid Binding ◽

Angiogenic Potential ◽

Adipose Tissue Engineering ◽

Angiogenesis Assay ◽

Soft Tissue Engineering

In vitro–generated soft tissue could provide alternate therapies for soft tissue defects. The aim of this study was to evaluate methacrylated gelatin/hyaluronan as scaffolds for soft tissue engineering and their interaction with human adipose–derived stem cells (hASCs). ASCs were incorporated into methacrylated gelatin/hyaluronan hydrogels. The gels were photocrosslinked with a lithium phenyl-2,4,6-trimethylbenzoylphosphinate photoinitiator and analyzed for cell viability and adipogenic differentiation of ASCs over a period of 30 days. Additionally, an angiogenesis assay was performed to assess their angiogenic potential. After 24 h, ASCs showed increased viability on composite hydrogels. These results were consistent over 21 days of culture. By induction of adipogenic differentiation, the mature adipocytes were observed after 7 days of culture, their number significantly increased until day 28 as well as expression of fatty acid binding protein 4 and adiponectin. Our scaffolds are promising as building blocks for adipose tissue engineering and allowed long viability, proliferation, and differentiation of ASCs.

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Electrospun hybrid nanofibrous meshes with adjustable performance for potential use in soft tissue engineering

Textile Research Journal ◽

10.1177/00405175211063904 ◽

2021 ◽

pp. 004051752110639

Author(s):

Ye Qi ◽

Huiyuan Zhai ◽

Yaning Sun ◽

Hongxing Xu ◽

Shaohua Wu ◽

...

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Soft Tissue ◽

Fiber Diameter ◽

Fiber Morphology ◽

Surface Hydrophilicity ◽

Nanofibrous Scaffolds ◽

Soft Tissue Engineering ◽

The Mean

Electrospun nanofibrous scaffolds have gained extensive attention in the fields of soft tissue engineering and regenerative medicine. In this study, a series of biodegradable nanofibrous meshes were fabricated by electrospinning poly(ε-caprolactone) (PCL) and poly( p-dioxanone) (PPDO) blends with various mass ratios. All the as-developed PCL/PPDO nanofibrous meshes possessed smooth and highly aligned fiber morphology. The mean fiber diameter was 521.5 ± 76.6 nm for PCL meshes and 485.8 ± 88.9 nm for PPDO meshes, and the mean fiber diameter seemed to present a decreasing tendency with the increasing of the PPDO component. For pure PCL meshes, the contact angle was about 117.5 ± 1.6°, the weight loss ratio was roughly 0.2% after 10 weeks of degradation, and the tensile strength was 41.2 ± 2.3 MPa in the longitudinal direction and 4.2 ± 0.1 MPa in the transverse direction. It was found that the surface hydrophilicity and in vitro degradation properties of PCL/PPDO meshes apparently increased, but the mechanical properties of PCL/PPDO meshes obviously decreased when more PPDO component was introduced. The biological tests showed that 4:1 PCL/PPDO nanofibrous meshes and 1:1 PCL/PPDO nanofibrous meshes could obviously promote the adhesion and proliferation of human adipose derived mesenchymal stem cells more than pure PCL and PPDO meshes and 1:4 PCL/PPDO meshes. The results demonstrated that it is feasible to adjust the surface hydrophilicity, degradation profile, and mechanical properties as well as biological properties of as-obtained nanofibrous meshes by blending PCL and PPDO components. This study provides meaningful reference and guidance for the design and development of PCL/PPDO hybrid nanofibrous scaffolds for soft tissue engineering research and application.

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