Mechanistic insight into the isomerization of allyl alcohol catalyzed by the Co(II)-PNP catalyst: Crucial role of spectator ligand

The chromatoid body (CB) is a specific cloud-like structure in the cytoplasm of haploid spermatids. Recent findings indicate that CB is identified as a male germ cell-specific RNA storage and processing center, but its function has remained elusive for decades. In somatic cells, KH-type splicing regulatory protein (KSRP) is involved in regulating gene expression and maturation of select microRNAs (miRNAs). However, the function of KSRP in spermatogenesis remains unclear. In this study, we showed that KSRP partly localizes in CB, as a component of CB. KSRP interacts with proteins (mouse VASA homolog (MVH), polyadenylate-binding protein 1 (PABP1) and polyadenylate-binding protein 2 (PABP2)), mRNAs (Tnp2 and Odf1) and microRNAs (microRNA-182) in mouse CB. Moreover, KSRP may regulate the integrity of CB via DDX5-miRNA-182 pathway. In addition, we found abnormal expressions of CB component in testes of Ksrp-knockout mice and of patients with hypospermatogenesis. Thus, our results provide mechanistic insight into the role of KSRP in spermatogenesis.

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Mechanistic insight into the ruthenium-catalysed anti-Markovnikov hydration of alkynes using a self-assembled complex: a crucial role for ligand-assisted proton shuttle processes

Dalton Transactions ◽

10.1039/c4dt00712c ◽

2014 ◽

Vol 43 (29) ◽

pp. 11277-11285 ◽

Cited By ~ 25

Author(s):

Bernhard Breit ◽

Urs Gellrich ◽

Timothy Li ◽

Jason M. Lynam ◽

Lucy M. Milner ◽

...

Keyword(s):

Experimental Study ◽

Crucial Role ◽

Ligand Complex ◽

Mechanistic Insight ◽

Self Assembled ◽

Insight Into

A combined computational and experimental study into the mechanism of the anti-Markovnikov hydration of phenylacetylene by a self-assembled ligand complex.

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Mechanistic insight into the role of metformin in Alzheimer's disease

Life Sciences ◽

10.1016/j.lfs.2021.120299 ◽

2022 ◽

pp. 120299

Author(s):

Mehdi Sanati ◽

Samaneh Aminyavari ◽

Amir R. Afshari ◽

Amirhossein Sahebkar

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Mechanistic Insight ◽

Insight Into

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Histone H3K23-specific acetylation by MORF is coupled to H3K14 acylation

Nature Communications ◽

10.1038/s41467-019-12551-5 ◽

2019 ◽

Vol 10 (1) ◽

Cited By ~ 4

Author(s):

Brianna J. Klein ◽

Suk Min Jang ◽

Catherine Lachance ◽

Wenyi Mi ◽

Jie Lyu ◽

...

Keyword(s):

Posttranslational Modification ◽

Memory Impairment ◽

Target Genes ◽

Epigenetic Mark ◽

Mechanistic Insight ◽

Genomic Analyses ◽

Insight Into

Abstract Acetylation of histone H3K23 has emerged as an essential posttranslational modification associated with cancer and learning and memory impairment, yet our understanding of this epigenetic mark remains insufficient. Here, we identify the native MORF complex as a histone H3K23-specific acetyltransferase and elucidate its mechanism of action. The acetyltransferase function of the catalytic MORF subunit is positively regulated by the DPF domain of MORF (MORFDPF). The crystal structure of MORFDPF in complex with crotonylated H3K14 peptide provides mechanistic insight into selectivity of this epigenetic reader and its ability to recognize both histone and DNA. ChIP data reveal the role of MORFDPF in MORF-dependent H3K23 acetylation of target genes. Mass spectrometry, biochemical and genomic analyses show co-existence of the H3K23ac and H3K14ac modifications in vitro and co-occupancy of the MORF complex, H3K23ac, and H3K14ac at specific loci in vivo. Our findings suggest a model in which interaction of MORFDPF with acylated H3K14 promotes acetylation of H3K23 by the native MORF complex to activate transcription.

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Role of non-electrostatic forces in antimicrobial potency of a dengue-virus derived fusion peptide VG16KRKP: Mechanistic insight into the interfacial peptide-lipid interactions

Biochimica et Biophysica Acta (BBA) - Biomembranes ◽

10.1016/j.bbamem.2019.01.011 ◽

2019 ◽

Vol 1861 (4) ◽

pp. 798-809 ◽

Cited By ~ 7

Author(s):

Dipita Bhattacharyya ◽

Minsoo Kim ◽

Kamal H. Mroue ◽

MinSeok Park ◽

Anuj Tiwari ◽

...

Keyword(s):

Dengue Virus ◽

Fusion Peptide ◽

Electrostatic Forces ◽

Lipid Interactions ◽

Mechanistic Insight ◽

Insight Into ◽

Antimicrobial Potency

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“Old Church Women”: An Insight into the Less Understood and Their Contribution to the Life of the Orthodox Church

Societies ◽

10.3390/soc9030063 ◽

2019 ◽

Vol 9 (3) ◽

pp. 63

Author(s):

Lavinia Țânculescu

Keyword(s):

Empirical Evidence ◽

Crucial Role ◽

Religious Tradition ◽

Spiritual Identity ◽

Orthodox Church ◽

Advantages And Disadvantages ◽

Romanian Orthodox Church ◽

Romanian Orthodox ◽

Insight Into

In this study, I aim to explore the role of old women in the life of the Christian Orthodox Church in the Romanian space. The analysis is based on empirical evidence (qualitative fieldwork and case studies) gathered between 2017 and 2019, and it mainly employs the framework of theory of tradition, and theories of attachment and of parent–infant relationship. I will show that old women going to church have a double role: To educate the community in keeping the religious tradition, and to initiate other members, especially the very young ones (blood-related or not), in the Romanian Orthodox faith. The paper discusses the advantages and disadvantages offered by both aforementioned roles, putting forth possible explanations for the tensions arising between generations. I conclude by underlining the crucial role that old women have in today’s struggle for survival of the Romanian Orthodox Church and in its spiritual identity.

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NUMB regulates the endocytosis and activity of the anaplastic lymphoma kinase in an isoform-specific manner

Journal of Molecular Cell Biology ◽

10.1093/jmcb/mjz003 ◽

2019 ◽

Vol 11 (11) ◽

pp. 994-1005 ◽

Cited By ~ 2

Author(s):

Ran Wei ◽

Xuguang Liu ◽

Courtney Voss ◽

Wentao Qin ◽

Lina Dagnino ◽

...

Keyword(s):

Cell Fate ◽

Receptor Tyrosine Kinase ◽

Anaplastic Lymphoma Kinase ◽

Degradation Pathway ◽

Anaplastic Lymphoma ◽

Mechanistic Insight ◽

Evolutionarily Conserved ◽

Specific Manner ◽

Insight Into

Abstract NUMB is an evolutionarily conserved protein that plays an important role in cell adhesion, migration, polarity, and cell fate determination. It has also been shown to play a role in the pathogenesis of certain cancers, although it remains controversial whether NUMB functions as an oncoprotein or tumor suppressor. Here, we show that NUMB binds to anaplastic lymphoma kinase (ALK), a receptor tyrosine kinase aberrantly activated in several forms of cancer, and this interaction regulates the endocytosis and activity of ALK. Intriguingly, the function of the NUMB–ALK interaction is isoform-dependent. While both p66-NUMB and p72-NUMB isoforms are capable of mediating the endocytosis of ALK, the former directs ALK to the lysosomal degradation pathway, thus decreasing the overall ALK level and the downstream MAP kinase signal. In contrast, the p72-NUMB isoform promotes ALK recycling back to the plasma membrane, thereby maintaining the kinase in its active state. Our work sheds light on the controversial role of different isoforms of NUMB in tumorigenesis and provides mechanistic insight into ALK regulation.

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