Comparative Analysis of T Cell Spatial Proteomics and the Influence of HIV Expression

8806 Russian patients demonstrate T cell count as better marker of COVID-19 clinical course severity than SARS-CoV-2 viral load

Scientific Reports ◽

10.1038/s41598-021-88714-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Konstantin S. Sharov

Keyword(s):

Viral Load ◽

Comparative Analysis ◽

T Cell ◽

Cell Count ◽

T Lymphocyte ◽

Clinical Course ◽

Time Interval ◽

Early Prediction ◽

Disease Prognosis ◽

Respiratory Index

AbstractThe article presents a comparative analysis of SARS-CoV-2 viral load (VL), T lymphocyte count and respiratory index PaO2:FiO2 ratio as prospective markers of COVID-19 course severity and prognosis. 8806 patients and asymptomatic carriers were investigated in time interval 15 March–19 December 2020. T cell count demonstrated better applicability as a marker of aggravating COVID-19 clinical course and unfavourable disease prognosis than SARS-CoV-2 VL or PaO2:FiO2 ratio taken alone. Using T cell count in clinical practice may provide an opportunity of early prediction of deteriorating a patient’s state.

Download Full-text

IMGT® Biocuration and Comparative Analysis of Bos taurus and Ovis aries TRA/TRD Loci

Genes ◽

10.3390/genes12010030 ◽

2020 ◽

Vol 12 (1) ◽

pp. 30

Author(s):

Perrine Pégorier ◽

Morgane Bertignac ◽

Viviane Nguefack Ngoune ◽

Géraldine Folch ◽

Joumana Jabado-Michaloud ◽

...

Keyword(s):

Immune Response ◽

Comparative Analysis ◽

T Cell ◽

T Cell Receptors ◽

Bos Taurus ◽

Homo Sapiens ◽

Adaptive Immune Response ◽

Ovis Aries ◽

Cell Receptors ◽

Adaptive Immune

The adaptive immune response provides the vertebrate immune system with the ability to recognize and remember specific pathogens to generate immunity, and mount stronger attacks each time the pathogen is encountered. T cell receptors are the antigen receptors of the adaptive immune response expressed by T cells, which specifically recognize processed antigens, presented as peptides by the highly polymorphic major histocompatibility (MH) proteins. T cell receptors (TR) are divided into two groups, αβ and γδ, which express distinct TR containing either α and β, or γ and δ chains, respectively. The TRα locus (TRA) and TRδ locus (TRD) of bovine (Bos taurus) and the sheep (Ovis aries) have recently been described and annotated by IMGT® biocurators. The aim of the present study is to present the results of the biocuration and to compare the genes of the TRA/TRD loci among these ruminant species based on the Homo sapiens repertoire. The comparative analysis shows similarities but also differences, including the fact that these two species have a TRA/TRD locus about three times larger than that of humans and therefore have many more genes which may demonstrate duplications and/or deletions during evolution.

Download Full-text

Comparative Analysis of Antibody Responses against HSP60, Invariant Surface Glycoprotein 70, and Variant Surface Glycoprotein Reveals a Complex Antigen-Specific Pattern of Immunoglobulin Isotype Switching during Infection byTrypanosoma brucei

Infection and Immunity ◽

10.1128/iai.68.2.848-860.2000 ◽

2000 ◽

Vol 68 (2) ◽

pp. 848-860 ◽

Cited By ~ 29

Author(s):

Magdalena Radwanska ◽

Stefan Magez ◽

Alain Michel ◽

Benoît Stijlemans ◽

Maurice Geuskens ◽

...

Keyword(s):

Comparative Analysis ◽

T Cell ◽

Antibody Responses ◽

Surface Glycoprotein ◽

Variant Surface Glycoprotein ◽

Mammalian Host ◽

Immune Recognition ◽

Isotype Switching ◽

Invariant Surface ◽

Immunoglobulin Isotype

ABSTRACT During Trypanosoma brucei infections, the response against the variant surface glycoprotein (VSG) of the parasite represents a major interaction between the mammalian host immune system and the parasite surface. Since immune recognition of other parasite derived factors also occurs, we examined the humoral host response against trypanosome heat shock protein 60 (HSP60), a conserved antigen with an autoimmune character. During experimental T. bruceiinfection in BALB/c mice, the anti-HSP60 response was induced when parasites differentiated into stumpy forms. This response was characterized by a stage-specific immunoglobulin isotype switching as well as by the induction of an autoimmune response. Specific recognition of trypanosome HSP60 was found to occur during the entire course of infection. Immunoglobulin G2a (IgG2a) and IgG2b antibodies, induced mainly in a T-cell-independent manner, were observed during the first peak of parasitemia, whereas IgG1 and IgG3 antibodies were found at the end of the infection, due to a specific T-cell-mediated response. Comparative analysis of the kinetics of anti-HSP60, anti-invariant surface glycoprotein 70 (ISG70), and anti-VSG antibody responses indicated that the three trypanosome antigens give rise to specific and independent patterns of immunoglobulin isotype switching.

Download Full-text