This article targets cell biologists who use fluorescence microscopy but lack automatic tools to summarize and manage their image datasets. When using microscopy to document a phenotype, multiple and random pictures are required to reflect the biological diversity of each imaged sample. Managing, integrating and summarizing the acquired data can be a daunting task that becomes extremely time-consuming unless one automatizes it. Unfortunately, if many biologists use microscopy, only a few have automatized procedures to cope with the data generated. For the majority of microscope users, the two developed complementary ImageJ plugins, PicPreview and PicSummary, will allow, in a few clicks and in an instant, to obtain an overview of all pictures taken for each sample of an experiment and a summary with one user-defined representative picture per sample. The plugins and a video tutorial, as well as demonstration pictures, are available as supplementary data at the journal website. PicPreview and PicSummary should save precious time in managing microscopy datasets and in preparing figures for publications.
Analysis of the ISIC Image Datasets: Usage, Benchmarks and Recommendations
