Investigation of biofilm formation ability, antimicrobial resistance and the staphylococcal cassette chromosome mec patterns of methicillin resistant Staphylococcus epidermidis with different sequence types isolated from children

ABSTRACT Methicillin-susceptible Staphylococcus aureus (MSSA) can arise from methicillin-resistant S. aureus (MRSA) following partial or complete excision of staphylococcal cassette chromosome mec (SCCmec). This study investigated whether multiresistant MSSA isolates from Irish hospitals, where MRSA has been endemic for decades, harbor SCCmec DNA. Twenty-five multiresistant MSSA isolates recovered between 2002 and 2006 were tested for SCCmec DNA by PCR and were genotyped by multilocus sequence typing and spa typing. All isolates lacked mecA. Three isolates (12%) harbored SCCmec DNA; two of these (genotype ST8/t190) harbored a 26-kb SCCmec IID (II.3.1.2) remnant that lacked part of mecI and all of mecR1, mecA, and IS431; the third isolate (ST8/t3209) harbored the SCCmec region from dcs to orfX. All three isolates were detected as MRSA using the BD GeneOhm and Cepheid's Xpert MRSA real-time PCR assays. Six isolates (ST8/t190, n = 4; ST5/t088, n = 2), including both isolates with the SCCmec IID remnant, harbored ccrAB4 with 100% identity to ccrAB4 from the Staphylococcus epidermidis composite island SCC-CI. This ccrAB4 gene was also identified in 23 MRSA isolates representative of ST8/t190-MRSA with variant SCCmec II subtypes IIA to IIE, which predominated previously in Irish hospitals. ccrAB4 was located 5,549 bp upstream of the left SCCmec junction in both the MRSA and MSSA isolates with SCCmec elements and remnants and 5,549 bp upstream of orfX in the four MSSA isolates with ccrAB4 only on an SCC-CI homologous region. This is the first description of a large SCCmec remnant with ccr and partial mec genes in MSSA and of the S. epidermidis SCC-CI and ccrAB4 genes in S. aureus.

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Determination of Antimicrobial Resistance Pattern in Methicillin-Resistant Staphylococcus saprophyticus and Staphylococcus epidermidis and Detection of Resistance Genes to Clindamycin and Erythromycin

Iranian Journal of Medical Microbiology ◽

10.30699/ijmm.12.3.169 ◽

2018 ◽

Vol 12 (3) ◽

pp. 169-178

Author(s):

Hamed Tahmasebi ◽

Shahnaz Dehbashi ◽

Mohammad Reza Arabestani ◽

◽

...

Keyword(s):

Antimicrobial Resistance ◽

Resistance Genes ◽

Staphylococcus Epidermidis ◽

Resistance Pattern ◽

Methicillin Resistant ◽

Staphylococcus Saprophyticus

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Umbelliferone Impedes Biofilm Formation and Virulence of Methicillin-Resistant Staphylococcus epidermidis via Impairment of Initial Attachment and Intercellular Adhesion

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2019.00357 ◽

2019 ◽

Vol 9 ◽

Cited By ~ 3

Author(s):

Thirukannamangai Krishnan Swetha ◽

Murugesan Pooranachithra ◽

Ganapathy Ashwinkumar Subramenium ◽

Velayutham Divya ◽

Krishnaswamy Balamurugan ◽

...

Keyword(s):

Staphylococcus Epidermidis ◽

Biofilm Formation ◽

Intercellular Adhesion ◽

Initial Attachment ◽

Methicillin Resistant

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Comparative Genotypes, Staphylococcal Cassette Chromosome mec (SCCmec) Genes and Antimicrobial Resistance amongst Staphylococcus epidermidis and Staphylococcus haemolyticus Isolates from Infections in Humans and Companion Animals

PLoS ONE ◽

10.1371/journal.pone.0138079 ◽

2015 ◽

Vol 10 (9) ◽

pp. e0138079 ◽

Cited By ~ 28

Author(s):

Brenda A. McManus ◽

David C. Coleman ◽

Emily C. Deasy ◽

Gráinne I. Brennan ◽

Brian O’ Connell ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Staphylococcus Epidermidis ◽

Companion Animals ◽

Staphylococcus Haemolyticus ◽

Staphylococcal Cassette Chromosome

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Antibiofilm effects of N,O-acetals derived from 2-amino-1,4-naphthoquinone are associated with downregulation of important global virulence regulators in methicillin-resistant Staphylococcus aureus

Scientific Reports ◽

10.1038/s41598-020-76372-z ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Juliana Silva Novais ◽

Mariana Fernandes Carvalho ◽

Mariana Severo Ramundo ◽

Cristiana Ossaille Beltrame ◽

Reinaldo Barros Geraldo ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Antimicrobial Resistance ◽

Biofilm Formation ◽

Methicillin Resistant Staphylococcus Aureus ◽

Biological Properties ◽

New Drugs ◽

Strong Impact ◽

Methicillin Resistant ◽

Great Demand

AbstractDespite the existing antibiotics, antimicrobial resistance is a major challenge. Consequently, the development of new drugs remains in great demand. Quinones is part of a broad group of molecules that present antibacterial activity besides other biological properties. The main purpose of this study was to evaluate the antibiofilm activities of synthetic N,O-acetals derived from 2-amino-1,4-naphthoquinone [7a: 2-(methoxymethyl)-amino-1,4-naphthoquinone; 7b: 2-(ethoxymethyl)-amino-1,4-naphthoquinone; and 7c: 2-(propynyloxymethyl)-amino-1,4-naphthoquinone] against methicillin-resistant Staphylococcus aureus (MRSA). The derivatives 7b and 7c, specially 7b, caused strong impact on biofilm accumulation. This inhibition was linked to decreased expression of the genes fnbA, spa, hla and psmα3. More importantly, this downregulation was paralleled by the modulation of global virulence regulators. The substitution of 2-ethoxymethyl (7b) in comparison with 2-propynyloxymethyl (7c) enhanced sarA-agr inhibition, decreased fnbA transcripts (positively regulated by sarA) and strongly impaired biofilm accumulation. Indeed, 7b triggered intensive autolysis and was able to eliminate vancomycin-persistent cells. Consequently, 7b is a promising molecule displaying not only antimicrobial effects, but also antibiofilm and antipersistence activities. Therefore, 7b is a good candidate for further studies involving the development of novel and more rational antimicrobials able to act in chronic and recalcitrant infections, associated with biofilm formation.

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Detection of methicillin-resistant Staphylococcus pseudintermedius ST169 and novel ST354 SCCmec II–III isolates related to the worldwide ST71 clone

Epidemiology and Infection ◽

10.1017/s0950268815001545 ◽

2015 ◽

Vol 144 (2) ◽

pp. 434-442 ◽

Cited By ~ 8

Author(s):

K. ISHIHARA ◽

A. KOIZUMI ◽

M. SAITO ◽

Y. MURAMATSU ◽

Y. TAMURA

Keyword(s):

Antimicrobial Resistance ◽

Housekeeping Genes ◽

Sequence Type ◽

Molecular Characteristics ◽

Clonal Lineage ◽

Staphylococcus Pseudintermedius ◽

Methicillin Resistant ◽

Antimicrobial Resistance Genes ◽

Human Healthcare ◽

Staphylococcal Cassette Chromosome

SUMMARYThe recent appearance of methicillin-resistant Staphylococcus pseudintermedius (MRSP) is a concern for both veterinary and human healthcare. MRSP clonal lineages with sequence type (ST) 71-spa t02-staphylococcal cassette chromosome mec (SCCmec) II–III and ST68-spa t06-SCCmec V have spread throughout Europe and North America, respectively. The current study compared the molecular characteristics of 43 MRSP isolates from dogs in Japan with those of MRSP from previous reports using multilocus sequence typing based on seven housekeeping genes, SCCmec typing, and detection of antimicrobial resistance genes. Three related clonal lineages, ST71, ST169, and the newly registered ST354, were observed in SCCmec II–III isolates from Japan, despite MRSP SCCmec II–III isolates being thought to belong to a single clonal lineage. The majority of SCCmec II–III isolates belonging to ST169 (9/11) and ST354 (3/3), but not ST71 (0/11), harboured tetM. Four STs were observed for the SCCmec V isolates; however, neither ST68 nor related STs were found in the Japanese MRSP isolates. In conclusion, MRSP SCCmec II–III isolates from Japan belonged to ST71 and related STs (ST169 and ST354). A variety of MRSP SCCmec V clones, including some novel clones, were identified.

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