Immunogenicity and protective response induced by recombinant Brucella abortus proteins Adk, SecB and combination of these two recombinant proteins against a virulent strain B. abortus 544 infection in BALB/c mice

ABSTRACT The P39 and the bacterioferrin (BFR) antigens of Brucella melitensis 16M were previously identified as T dominant antigens able to induce both delayed-type hypersensivity in sensitized guinea pigs and in vitro gamma interferon (IFN-γ) production by peripheral blood mononuclear cells from infected cattle. Here, we analyzed the potential for these antigens to function as a subunitary vaccine against Brucella abortus infection in BALB/c mice, and we characterized the humoral and cellular immune responses induced. Mice were injected with each of the recombinant proteins alone or adjuvanted with either CpG oligodeoxynucleotides (CpG ODN) or non-CpG ODN. Mice immunized with the recombinant antigens with CpG ODN were the only group demonstrating both significant IFN-γ production and T-cell proliferation in response to either Brucella extract or to the respective antigen. The same conclusion holds true for the antibody response, which was only demonstrated in mice immunized with recombinant antigens mixed with CpG ODN. The antibody titers (both immunoglobulin G1 [IgG1] and IgG2a) induced by P39 immunization were higher than the titers induced by BFR (only IgG2a). Using a B. abortus 544 challenge, the level of protection was analyzed and compared to the protection conferred by one immunization with the vaccine strain B19. Immunization with P39 and CpG ODN gave a level of protection comparable to the one conferred by B19 at 4 weeks postchallenge, and the mice were still significantly protected at 8 weeks postchallenge, although to a lesser extent than the B19-vaccinated group. Intriguingly, no protection was detected after BFR vaccination. All other groups did not demonstrate any protection.

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Evaluation of Th1/Th2-Related Immune Response against Recombinant Proteins of Brucella abortus Infection in Mice

Journal of Microbiology and Biotechnology ◽

10.4014/jmb.1512.12046 ◽

2016 ◽

Vol 26 (6) ◽

pp. 1132-1139 ◽

Cited By ~ 12

Author(s):

Young Bin Im ◽

Woo Bin Park ◽

Myunghwan Jung ◽

Suk Kim ◽

Han Sang Yoo

Keyword(s):

Immune Response ◽

Recombinant Proteins ◽

Brucella Abortus

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THE PATHOGENESIS OF EXPERIMENTAL BRUCELLOSIS IN VIRGIN HEIFERS WITH AND WITHOUT CONTINUOUS PROGESTERONE TREATMENT

Journal of Endocrinology ◽

10.1677/joe.0.0200345 ◽

1960 ◽

Vol 20 (4) ◽

pp. 345-354 ◽

Cited By ~ 8

Author(s):

J. M. PAYNE

Keyword(s):

Standard Method ◽

Brucella Abortus ◽

Lymphoid Tissue ◽

Genital Tract ◽

Virulent Strain ◽

The Body ◽

Uterine Lumen ◽

Progesterone Treatment

SUMMARY Daily injections of progesterone were given to six adult heifers. These, and six untreated but otherwise similar animals, were infected by a standard method with the virulent strain 544 of Brucella abortus. The pathogenesis of the disease, as measured by B. abortus counts in various tissues and organs, and associated histopathology, was found to be less progressive and milder than in the pregnant cow. In the non-gravid animals the counts of B. abortus in the various tissues were very low and the infection did not disseminate very widely in the body. The uteri remained free from infection. In addition, the lesions in the lymphoid tissue were milder than those found previously in similarly infected pregnant animals. Progesterone administration exerted a profound effect on the genital tract. Oestrous cycles ceased and follicles in the ovaries became atretic; the endometrial glands secreted a material which accumulated in the uterine lumen and a firm mucus seal developed in the cervix. Progesterone, however, exerted no effect on the pathogenesis of brucellosis. It is concluded that progesterone is unlikely to be responsible for the susceptibility of the pregnant cow to brucellosis.

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An RNA Vaccine Based on Recombinant Semliki Forest Virus Particles Expressing the Cu,Zn Superoxide Dismutase Protein of Brucella abortus Induces Protective Immunity in BALB/c Mice

Infection and Immunity ◽

10.1128/iai.73.6.3294-3300.2005 ◽

2005 ◽

Vol 73 (6) ◽

pp. 3294-3300 ◽

Cited By ~ 14

Author(s):

Angel A. Oñate ◽

Gabriel Donoso ◽

Gustavo Moraga-Cid ◽

Hugo Folch ◽

Sandra Céspedes ◽

...

Keyword(s):

Superoxide Dismutase ◽

T Cell ◽

Brucella Abortus ◽

Semliki Forest Virus ◽

Cytotoxic T Lymphocyte ◽

Virulent Strain ◽

Interleukin 4 ◽

Cell Immune Response ◽

Virus Particles

ABSTRACT We constructed infectious but replication-deficient Semliki Forest virus (SFV) particles carrying recombinant RNA encoding Brucella abortus Cu,Zn superoxide dismutase (SOD). The recombinant SFV particles (SFV-SOD particles) were then evaluated for their ability to induce a T-cell immune response and to protect BALB/c mice against a challenge with B. abortus 2308. Intraperitoneal injection of mice with recombinant SFV-SOD particles did not lead to the induction of SOD-specific antibodies, at least until week 6 after immunization (the end of the experiment). In vitro stimulation of splenocytes from the vaccinated mice with either recombinant Cu,Zn SOD (rSOD) or crude Brucella protein resulted in a T-cell proliferative response and the induction of gamma interferon secretion but not interleukin-4. In addition, the splenocytes exhibited significant levels of cytotoxic T-lymphocyte activity against Brucella-infected cells. The SFV-SOD particles, but not the control virus particles, induced a significant level of protection in BALB/c mice against challenge with B. abortus virulent strain 2308. These findings indicated that an SFV-based vector carrying the SOD gene has potential for use as a vaccine to induce resistance against B. abortus infections.

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Draft Genome Sequence of Brucella abortus Virulent Strain 544

Genome Announcements ◽

10.1128/genomea.00419-15 ◽

2015 ◽

Vol 3 (3) ◽

Cited By ~ 2

Author(s):

D. K. Singh ◽

Ashok Kumar ◽

A. K. Tiwari ◽

Jagadesan Sankarasubramanian ◽

Udayakumar S. Vishnu ◽

...

Keyword(s):

Genome Sequence ◽

Brucella Abortus ◽

Virulent Strain ◽

Draft Genome ◽

Draft Genome Sequence

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Characterization of Specific Immune Responses of Mice Inoculated with Recombinant Vaccinia Virus Expressing an 18-Kilodalton Outer Membrane Protein of Brucella abortus

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.7.1.114-118.2000 ◽

2000 ◽

Vol 7 (1) ◽

pp. 114-118 ◽

Cited By ~ 31

Author(s):

Ramesh Vemulapalli ◽

Silvio Cravero ◽

Christine L. Calvert ◽

Thomas E. Toth ◽

Nammalwar Sriranganathan ◽

...

Keyword(s):

Membrane Protein ◽

Vaccinia Virus ◽

Outer Membrane ◽

Outer Membrane Protein ◽

Brucella Abortus ◽

Virulent Strain ◽

Shuttle Vector ◽

Protein Fusion

ABSTRACT Using the shuttle vector pMCO2 and the vaccinia virus wild-type WR strain, we constructed a recombinant virus expressing an 18-kDa outer membrane protein of Brucella abortus. BALB/c mice inoculated with this virus produced 18-kDa protein-specific antibodies, mostly of immunoglobulin G2a isotype, and in vitro stimulation of splenocytes from these mice with purified maltose binding protein–18-kDa protein fusion resulted in lymphocyte proliferation and gamma interferon production. However, these mice were not protected against a challenge with the virulent strain B. abortus2308. Disruption of the 18-kDa protein's gene in vaccine strainB. abortus RB51 did not affect either the strain's protective capabilities or its in vivo attenuation characteristics. These observations suggest that the 18-kDa protein plays no role in protective immunity.

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Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia

Biosensors ◽

10.3390/bios9040115 ◽

2019 ◽

Vol 9 (4) ◽

pp. 115

Author(s):

Lyubov V. Barshevskaya ◽

Dmitriy V. Sotnikov ◽

Anatoly V. Zherdev ◽

Bekbolat B. Khassenov ◽

Kayrat K. Baltin ◽

...

Keyword(s):

Recombinant Proteins ◽

Brucella Abortus ◽

Bovine Leukemia Virus ◽

Leukemia Virus ◽

Chimeric Protein ◽

Test System ◽

Immunochromatographic Test ◽

Test Results ◽

Bovine Brucellosis ◽

False Positive Test

An immunochromatographic test system has been developed for the simultaneous rapid multiplex serodiagnostics of bovine brucellosis, tuberculosis, and leukemia. The test system is based on the use of a conjugate of gold nanoparticles with the chimeric protein Cysteine-A/G and three analytical zones with immobilized pathogen antigens: Brucella abortus lipolysaccharide, recombinant proteins MPB64 and MPB83-MPB63 of Mycobacterium bovis, and recombinant protein p24 of the bovine leukemia virus. Prototypes of the test system were tested on 98 samples of sera from healthy and infected animals. The diagnostic sensitivity of the developed test system was 92% for brucellosis, 92% for tuberculosis, and 96% for leukemia. False positive test results were not observed.

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