A systematic review and meta-analysis of antibiotic resistance patterns, and the correlation between biofilm formation with virulence factors in uropathogenic E. coli isolated from urinary tract infections

2020 ◽  
Vol 144 ◽  
pp. 104196 ◽  
Author(s):  
Fei Zhao ◽  
Huanxin Yang ◽  
Dezhong Bi ◽  
Azad Khaledi ◽  
Mingqi Qiao
Keyword(s):  
Systematic Review ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Meta Analysis ◽  
E Coli ◽  
Resistance Patterns ◽  
Tract Infections ◽  
Antibiotic Resistance Patterns

scholarly journals Evaluation of Biofilm Formation and Virulence Genes and Association with Antibiotic Resistance Patterns of Uropathogenic Escherichia coli Strains in Southwestern Iran

2021 ◽  
Vol 14 (9) ◽  
Author(s):  
Mostafa Boroumand ◽  
Asghar Sharifi ◽  
Mohammad Amin Ghatei ◽  
Mohsen Sadrinasab
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Biofilm Formation ◽  
Virulence Genes ◽  
Uropathogenic Escherichia Coli ◽  
E Coli ◽  
Resistance Patterns ◽  
Pcr Method ◽  
Antibiotic Resistance Patterns

Background: Uropathogenic Escherichia coli (UPEC) strains, encoding superficial and secretory virulence factors, can lead to colonization and facilitation of bacterial growth in the host urinary tract, causing Urinary Tract Infection (UTI). Objectives: This study determined the ability of biofilm formation by the Congo red agar (CRA) method, the presence of virulence genes using the multiplex polymerase chain reaction (PCR) method, and the relationship between biofilm formation and antibiotic resistance patterns and virulence genes in E. coli clinical isolates in Yasuj. Methods: This cross-sectional study was performed on 144 UPEC isolates collected in 2017. Biofilm formation was detected by the CRA phenotypic assay and virulence factors by the multiplex PCR method. Antibiotic resistance tests were performed by the Kirby-Bauer method. Results: Out of 144 isolates of E. coli, 22 (19.4%) isolates showed to be strong biofilm producers, 27 (23.8%) moderate biofilm producers, and 64 (56.3%) weak biofilm producers. A significant relationship was observed between biofilm-producing strains and resistance to ampicillin (P = 0.020) and cotrimoxazole (P = 0.038). The virulence genes in strong biofilm producers included iutA (95%), FimH (93%), ompT (90%), PAI (90%), and TraT (81%) genes. The phylogroup B2 carried the most virulence genes. A significant correlation was observed between E. coli phylogenetic groups and aer (P = 0.019), iroN (P = 0.042), and ompT (P = 0.032) virulence genes. Conclusions: The results of this study showed a high prevalence of virulence genes, and antibiotic-resistant E. coli strains capable of biofilm formation. The results of this study may help elucidate the pathogenesis of UPEC and facilitate better treatment strategies for patients with UTIs in this geographic area.

scholarly journals Antimicrobial susceptibility patterns of community-acquired uropathogens in Tehran, Iran

2010 ◽  
Vol 4 (04) ◽  
pp. 202-206 ◽  
Author(s):  
Nasim Kashef ◽  
Gholamreza Esmaeeli Djavid ◽  
Sahba Shahbazi
Keyword(s):  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Pathogenic Bacteria ◽  
Antimicrobial Treatment ◽  
Urine Samples ◽  
Resistance Testing ◽  
E Coli ◽  
Resistance Patterns ◽  
Tract Infections ◽  
Antibiotic Resistance Patterns

Background: Antibiotic resistance of urinary tract pathogens has increased worldwide. Knowledge of the antibiotic resistance patterns of uropathogens in specific geographical locations is an important factor for choosing an appropriate empirical antimicrobial treatment. The aim of this study was to provide information regarding local resistance patterns of urinary pathogens to the commonly used antibiotics in Tehran, Iran. Methodology: Urine samples collected and submitted to two pathobiology laboratories in Tehran were identified by conventional methods over a period of three years (December 2006 to May 2009). Antimicrobial resistance testing was performed by the standard disk diffusion technique in accordance with the recommendations of the Clinical and Laboratory Standards Institute. Results: Of the total 13,333 mid-stream urine samples collected from suspected cases of urinary tract infection, 840 (6.3%) were positive for pathogenic bacteria. Escherichia coli (E. coli) was the most common isolate (68.8%) followed by Proteus spp. (12.4%), and Klebsiella spp. (9.6%). E. coli isolates were mostly susceptible to nitrofurantoin (71.3%), followed by ciprofloxacin (68.1%); however, only 38.2% of E. coli isolates were susceptible to trimethoprim-sulfamethoxazole. Conclusion: Nitrofurantoin may be considered as a first-line empiric antibacterial agent for urinary tract infections in outpatients in Tehran, Iran.

scholarly journals Antibiotic Resistance Patterns of Outpatient Pediatric Urinary Tract Infections

2013 ◽  
Vol 190 (1) ◽  
pp. 222-227 ◽  
Author(s):  
Rachel S. Edlin ◽  
Daniel J. Shapiro ◽  
Adam L. Hersh ◽  
Hillary L. Copp
Keyword(s):  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Resistance Patterns ◽  
Tract Infections ◽  
Antibiotic Resistance Patterns
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