Characterization of the metabolite produced by Mycosphaerella pinodes, the causal agent of mycosphaerella blight on field peas (Pisum sativum L.)

Fungi associated with the ascochyta blight complex of field peas were isolated from 436 of 691 seedlots tested. Of the fungi detected, 94.8% of isolates were Mycosphaerella pinodes, 4.2% Phoma medicaginis, and 1.0% Ascochyta pisi. The levels of infestation of seed varied considerably from year to year and between seedlots, depending on the amount of rainfall between flowering and maturity. Within a particular pea-growing region, the level of seed-borne infection was often highest in seed from crops harvested latest. In addition, crops sown early were usually more severely affected by disease than late-sown crops, and this resulted in higher levels of seed infection. There was no correlation between the level of seed infestation by M. pinodes and the severity of ascochyta blight; however, where the level of seed infection was high (>11%) there was a significant reduction in emergence, which caused a reduction in grain yield. It may therefore be possible to use seed with high levels of seed-borne ascochyta blight fungi, provided the seeding rate is increased to compensate for poor emergence.

Download Full-text

Assessment of the feeding value of South Dakota-grown field peas (Pisum sativum L.) for growing pigs12

Journal of Animal Science ◽

10.2527/2004.8292568x ◽

2004 ◽

Vol 82 (9) ◽

pp. 2568-2578 ◽

Cited By ~ 49

Author(s):

H. H. Stein ◽

G. Benzoni ◽

R. A. Bohlke ◽

D. N. Peters

Keyword(s):

Pisum Sativum ◽

South Dakota ◽

Pisum Sativum L ◽

Field Peas ◽

Feeding Value

Download Full-text

Proximate and mineral composition of field peas

Canadian Journal of Plant Science ◽

10.4141/p96-059 ◽

1997 ◽

Vol 77 (1) ◽

pp. 101-103 ◽

Cited By ~ 4

Author(s):

T. D. Warkentin ◽

A. G. Sloan ◽

S. T. Ali-Khan

Keyword(s):

Pisum Sativum ◽

Key Words ◽

Mineral Composition ◽

Seed Yield ◽

Total Protein ◽

Field Pea ◽

Pisum Sativum L ◽

Field Peas ◽

Pea Seeds ◽

Proximate And Mineral Composition

Field pea seeds from 10 cultivars grown at two locations in Manitoba in 1986 and 1987 were analyzed for proximate and mineral profiles. Cultivars differed significantly in their level of total protein, crude fat, ADF, and all minerals tested. However, differences were not extremely large and were comparable to European reports. Location-year also had a significant effect on the levels of total protein, ADF, and all minerals tested. In most cases, the warmest location-year produced relatively higher levels of minerals, ash, and total protein, and lower seed yield than the coolest location-year. Key words: Field pea, Pisum sativum L., mineral

Download Full-text

Cloning and characterization of 66 kDa streptavidin-binding peptides (SBP) of Pisum sativum L. embryo specific to var. Alaska

BIOCELL ◽

10.32604/biocell.2019.06814 ◽

2019 ◽

Vol 43 (3) ◽

pp. 155-166

Author(s):

Mahmoud MOUSTAFA ◽

Saad ALAMRI ◽

Tarek TAHA ◽

Ali SHATI ◽

Sulaiman ALRUMMAN ◽

...

Keyword(s):

Pisum Sativum ◽

Pisum Sativum L ◽

Binding Peptides ◽

Streptavidin Binding

Download Full-text

Candidate genes for quantitative resistance to Mycosphaerella pinodes in pea (Pisum sativum L.)

Theoretical and Applied Genetics ◽

10.1007/s00122-006-0492-y ◽

2007 ◽

Vol 114 (6) ◽

pp. 971-984 ◽

Cited By ~ 52

Author(s):

S. Prioul-Gervais ◽

G. Deniot ◽

E-M. Receveur ◽

A. Frankewitz ◽

M. Fourmann ◽

...

Keyword(s):

Pisum Sativum ◽

Candidate Genes ◽

Quantitative Resistance ◽

Mycosphaerella Pinodes ◽

Pisum Sativum L

Download Full-text

Introgression of the Afila Gene into Climbing Garden Pea (Pisum sativum L.)

Agronomy ◽

10.3390/agronomy10101537 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1537

Author(s):

Oscar Checa ◽

Marino Rodriguez ◽

Xingbo Wu ◽

Matthew Blair

Keyword(s):

Pisum Sativum ◽

Production Costs ◽

Yield Potential ◽

Recurrent Parent ◽

Linkage Drag ◽

Progeny Testing ◽

High Biomass ◽

Garden Pea ◽

Pisum Sativum L ◽

Field Peas

The pea (Pisum sativum L.) is one of the most important crops in temperate agriculture around the world. In the tropics, highland production is also common with multiple harvests of nearly mature seeds from climbing plant types on trellises. While the leafless variant caused by the afila gene is widely used in developing row-cropped field peas in Europe, its use for trellised garden peas has not been reported. In this study we describe a pea breeding program for a high-elevation tropical environment in the Department of Nariño in Colombia, where over 16,000 hectares of the crop are produced. The most widespread climbing varieties in the region are ‘Andina’ and ‘Sindamanoy’, both of which have high-biomass architecture with abundant foliage. They are prone to many diseases, but preferred by farmers given their long production season. This plant type is expensive to trellis, with wooden posts and plastic strings used for vine staking constituting 52% of production costs. The afila trait could reduce these costs by creating interlocking plants as they do in field peas. Therefore, our goal for this research was to develop a rapid breeding method to introduce the recessive afila gene, which replaces leaves with tendrils, into the two commercial varieties used as recurrent parents (RPs) with three donor parents (DPs)—‘Dove’, ‘ILS3575′ and ‘ILS3568′—and to measure the effect on plant height (PH) and yield potential. Our hypothesis was that the afila gene would not cause linkage drag while obtaining a leafless climbing pea variety. Backcrossing was conducted without selfing for two generations and plants were selected to recover recurrent parent characteristics. Chi-square tests showed a ratio of 15 normal leaved to one afila leaved in the BC2F2 plants, and 31:1 in the BC3F2 generation. Selecting in the last of these generations permitted a discovery of tall climbing plants that were similar to those preferred commercially, but with the stable leafless afila. The method saved two seasons compared to the traditional method of progeny testing before each backcross cycle; the peas reached the BC2F2 generation in five seasons and the BC3F2 in seven seasons. This is advantageous with trellised peas that normally require half a year to reach maturity. Leafless garden peas containing the afila gene were of the same height as recurrent parents and, by the third backcross, were equally productive, without the high biomass found in the traditional donor varieties. The value of the afila gene and the direct backcrossing scheme is discussed in terms of garden pea improvement and crop breeding.

Download Full-text

AAC Asher field pea

Canadian Journal of Plant Science ◽

10.1139/cjps-2020-0190 ◽

2020 ◽

Author(s):

Dengjin Bing ◽

Don Beauchesne ◽

Debra L. McLaren ◽

Richard Cuthbert ◽

Hamid Naeem

Keyword(s):

Powdery Mildew ◽

Pisum Sativum ◽

Seed Weight ◽

Field Pea ◽

Mycosphaerella Pinodes ◽

Western Canada ◽

Erysiphe Pisi ◽

Lodging Resistance ◽

Pisum Sativum L ◽

Thousand Seed Weight

AAC Asher is a semi-leafless, medium to large seeded yellow cotyledon field pea (Pisum sativum L.) variety developed at Agriculture and Agri-Food Canada, Lacombe Research and Development Centre, Lacombe, Alberta, Canada. It has high yielding potential, early to medium maturity and good lodging resistance. AAC Asher has maturity of 94 days, and one-thousand-seed weight of 257 g. AAC Asher is resistant to powdery mildew (caused by Erysiphe pisi Syd.), moderately tolerant to mycosphaerella blight (caused by Mycosphaerella pinodes) and fusarium wilt (caused by Fusarium oxysporum). AAC Asher is adapted to all field growing regions in western Canada.

Download Full-text

ENERGY DISPERSIVE X-RAY ANALYSIS OF FIELD PEAS WITH DIFFERENT COOKING QUALITY

Canadian Journal of Plant Science ◽

10.4141/cjps83-137 ◽

1983 ◽

Vol 63 (4) ◽

pp. 1071-1074 ◽

Cited By ~ 1

Author(s):

J. CHONG ◽

S. T. ALI-KHAN ◽

B. B. CHUBEY ◽

G. H. GUBBELS

Keyword(s):

Statistical Analysis ◽

Pisum Sativum ◽

Cooking Quality ◽

Energy Dispersive ◽

Protein Bodies ◽

X Ray ◽

Pisum Sativum L ◽

Field Peas ◽

Freeze Dried ◽

High Concentrations

An energy dispersive X-ray (EDX) analytical method was used to study the freeze-dried powder of seeds of field peas (Pisum sativum L.) with good and poor cooking quality. EDX analysis of the electron-dense particles in the freeze-dried powder revealed the presence of high concentrations of Mg, P, and K, suggesting that the particles were protein bodies. Seeds with different cooking quality were compared with respect to the ratios of these elements in the dense particles. Statistical analysis indicated a significant correlation between these ratios and cooking quality.Key words: Pisum sativum, protein bodies, elemental analysis

Download Full-text

Purification and Characterization of Ornithine Transcarbamylase from Pea (Pisum sativum L.)

PLANT PHYSIOLOGY ◽

10.1104/pp.96.1.262 ◽

1991 ◽

Vol 96 (1) ◽

pp. 262-268 ◽

Cited By ~ 10

Author(s):

Robert D. Slocum ◽

David P. Richardson

Keyword(s):

Pisum Sativum ◽

Ornithine Transcarbamylase ◽

Purification And Characterization ◽

Pisum Sativum L

Download Full-text

Physiological and Biochemical Characterization of the GABA Shunt Pathway in Pea (Pisum sativum L.) Seedlings Under Drought Stress

Horticulturae ◽

10.3390/horticulturae7060125 ◽

2021 ◽

Vol 7 (6) ◽

pp. 125

Author(s):

Nisreen A. AL-Quraan ◽

Zakaria I. Al-Ajlouni ◽

Nima F. Qawasma

Keyword(s):

Drought Stress ◽

Pisum Sativum ◽

Water Availability ◽

Dry Weight ◽

Gaba Shunt ◽

Pisum Sativum L ◽

Pea Seedlings ◽

Mda Content ◽

Carbohydrate Levels

The physiological and biochemical role of the γ-aminobutyric acid (GABA) shunt pathway in green pea seedlings (Pisum sativum L.) was studied in response to soil water holding capacity levels: 80%, 60%, 40%, 20%, and 10% grown under continuous light at 25 °C for 7 days and 14 days, separately. Characterization of seeds germination pattern, seedlings growth (plant height, fresh and dry weight, and chlorophyll contents), GABA shunt metabolite (GABA, glutamate, and alanine) levels, total protein and carbohydrate levels, and oxidative damage (MDA level) were examined. Data showed a significant effect of drought stress on seed germination, plant growth, GABA shunt metabolites level, total protein and carbohydrate contents, and MDA level. A significant decline in seed germination percentage was recorded at a 20% drought level, which indicated that 20% of soil water holding capacity is the threshold value of water availability for normal germination after 14 days. Seedling fresh weight, dry weight, and plant height were significantly reduced with a positive correlation as water availability was decreased. There was a significant decrease with a positive correlation in Chl a and Chl b contents in response to 7 days and 14 days of drought. GABA shunt metabolites were significantly increased with a negative correlation as water availability decreased. Pea seedlings showed a significant increase in protein content as drought stress was increased. Total carbohydrate levels increased significantly when the amount of water availability decreased. MDA content increased slightly but significantly after 7 days and sharply after 14 days under all water stress levels. The maximum increase in MDA content was observed at 20% and 10% water levels. Overall, the significant increases in GABA, protein and carbohydrate contents were to cope with the physiological impact of drought stress on Pisum sativum L. seedlings by maintaining cellular osmotic adjustment, protecting plants from oxidative stress, balancing carbon and nitrogen (C:N) metabolism, and maintaining cell metabolic homeostasis and cell turgor. The results presented in this study indicated that severe (less than 40% water content of the holding capacity) and long-term drought stress should be avoided during the germination stage to ensure proper seedling growth and metabolism in Pisum sativum L.

Download Full-text